• Molecules and Cells
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• 제27권2호
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• pp.205-209
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• 2009

The loci of the 5S and 45S rRNA genes were localized on chromosomes in five species of Capsicum, namely, annuum, chacoense, frutescens, baccatum, and chinense by FISH. The 5S rDNA was localized to the distal region of one chromosome in all species observed. The number of 45S rDNA loci varied among species; one in annuum, two in chacoense and frutescens, and chinense, and four in baccatum, with the exceptions that 'CM334' of annuum had three loci and 'tabasco' of frutescens gad one locus. 'CM334'-derived BAC clones, 384B09 and 365P05, were screened with 5S rDNA as a probe, and BACs 278M03 and 262A23 were screened with 25S rDNA as a probe. Both ends of these BAC clones were sequenced. FISH with these BAC probes on pachytenes from 'CM334' plant showed one 5S rDNA locus and three 45S rDNA loci, consistent with the patterns on the somatic chromosomes. The 5S rDNA probe was also applied on extended DNA fibers to reveal that its coverage measured as long as 0.439 Mb in the pepper genome. FISH techniques applied on somatic and meiotic chromosomes and fibers have been established for chili to provide valuable information about the copy number variation of 45S rDNA and the actual physical size of the 5S rDNA in chili.

• 한국약용작물학회지
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• 제12권1호
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• pp.60-66
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• 2004

한국산 옻나무속 6종에 대하여 분자식물학적 방법으로 계통유연관계를 확인하기 위하여, nrDNA의 ITS 구간과 cpDNA rbcL 염기서열을 사용하여 계통분석한 결과 ITS 1의 길이는 $246{\sim}253\;bp$이었고, ITS 2는 $234{\sim}244\;bp$이었다. ITS 1의 길이는 Rhus sylvestris와 R. succedanea에서 246 bp로 가장 작았으며, R. verniciflua에서 253 bp로 가장 긴 것으로 나타났다. ITS 2의 길이는 R. verniciflua가 234 bp로 가장 짧았으며, R. trichocarpr가 244 bp로 가장 길게 나타났다. 이들 분류군의 G+C Content는 ITS 1에서는 $58.0{\sim}68.13%$의 범위를 나타냈고, ITS 2에서는 $59.75{\sim}68.46%$로 나타나 두 구간이 비슷한 비율을 보이고 있었다. ITS 1에서의 G+C content는 R. sylvestris가 58.0%로 가장 낮았으며, 가장 높은 값은 R. ambigua가 68.13%로 확인되었다. ITS 2에서는 외군인 Cotinus coggygria가 59.75%로 가장 낮았으며, R. ambigua가 68.46%로 가장 높게 나타났다. 한국산 옻나무 속에서 ITS 염기서열은 일반적으로 피자식물이 갖는 G+C content 범위 안에 포함되는 것으로 확인되었다. 한편, rbcL의 길이는 1,428 bp로 모든 종에서 동일하였다. 또한 rbcL의 G+C content는 $43.56%{\sim}43.77%$로 나타나 종간에 거의 차이가 없음을 확인하였다. 연구결과 rbcL gene은 옻나무속의 종간 계통유연관계를 해석하는데 유용하지 않았으며, ITS 1 구간의 염기서열 변이는 향후 옻나무속을 분류할 때 신속하게 분류할 수 있는 분류 marker로 이용할 수 있다고 판단되었다.

• 한국버섯학회지
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• 제12권4호
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• pp.251-257
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• 2014

수집한 46개의 균주를 배양하여 rDNA의 ITS 염기서열 분석으로 유전적 계통분류 한 결과, Heiricum속이 아닌 균주가 2균주가 있었음을 확인하였다. 본 결과를 바탕으로 효능이 있는 것으로 밝혀진 H.erinaceus와 가까운 유연관계를 지니는 종들을 위주로 계통별로 균사 생장실험, 자실체 발생조사 등을 연구할 예정이다. 또한 H.erinaceus은 아니지만, 생리활성효능이 뛰어난 종의 유무를 테스트하여 기존의 품종보다 효능 면에서 뛰어난 균주를 선발 교잡할 예정이다. 이와 같은 연구를 통해 우수품종을 선발하여 최적 환경조건 테스트까지 거친다면 노루궁뎅이 버섯 소비시장 구축에 도움이 되리라 생각된다.

• Mycobiology
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• 제29권3호
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• pp.121-131
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• 2001

This study was carried out to identify the phylogenetic relationships among several caterpillar fungi by comparing the sequences of internal transcribed spacer regions(ITS1 and ITS2) and 5.8S ribosomal DNA(rDNA) repeat unit. The sequences of ITS1, ITS2, and the 5.8S rDNA from 10 strains of Cordyceps species, 12 strains of Paecilomyces, 3 strains of Beauveria, 2 strains of Metarhizium and 1 strains of Hirsutella were amplified, determined and compared with the previously known Cordyceps species. The sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites could be found. In the phylogenetic tree, the species generally divided into three clusters, supported by their morphology and/or host ranges. The 5.8S rDNA and TTS1 sequences among 10 species of Cordyceps militaris were identical and only one base pair in ITS2 sequence was different. Cordyceps sinensis and Cordyceps ophioglossoides were also clearly different, although they belonged to the same cluster. The Geniank database search of species revealed sister taxa of an entomogenous fungus. Metarhizium was used as an putgroup in all taxa.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.145.2-145
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• 1995

• Journal of Species Research
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• 제12권4호
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• pp.307-312
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• 2023

Oxytricha multilineata, Mixophrya pantanalensis pantanalensis, and Caudiurostyla sinensis were isolated from soil samples collected from Cheongju-si and Yeoju-si, confirmed as new to South Korea. Oxytricha multilineata was distinguished from other congeners by seven dorsal kineties and dorsal bristles about 15 ㎛ long. Mixophrya pantanalensis pantanalensis was characterized by five to seven lithosomes and six dorsal kineties. Caudiurostyla sinensis was characterized by colorless cortical granules present, 10-14 midventral pairs, 7-9 left and 6-9 right marginal rows and four or five dorsal kineties. We determined the ribosomal DNA sequences (including 18S rDNA, ITS1, 5.8S rDNA, ITS2, and partial 28S rDNA) from above three species. And the genetic distances were compared with their congeners.

• 생명과학회지
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• 제25권2호
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• pp.243-248
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• 2015

국내에 자생 중인 도깨비엉겅퀴(Cirsium shantarense), 물엉겅퀴(Cirsium nipponicum), 정영엉겅퀴(Cirsium chanroenicum) 각 1개체 및 엉겅퀴(Cirsium japonicum) 8개체를 전국의 여러 지점에서 채집하였다. 채집된 식물들의 genomic DNA를 분리하여서 18S rDNA, ITS1, 5.8S rDNA, ITS2 및 28S rDNA의 일부를 증폭하고, 그 염기서열 및 Genbank에 등록되어 있는 다른 Cirsium 속 식물들의 염기서열을 분자유전학적으로 비교하여 유연관계를 분석하였다. 그 결과 고려엉겅퀴, 정영엉겅퀴, 물엉겅퀴, 큰엉겅퀴 및 엉겅퀴 종의 식물들은 모두 뚜렷이 독립된 그룹을 형성하고 있는 것을 알 수 있었다. 그러나 도깨비엉겅퀴는 비록 두화가 아래를 향하고 있지만 두화가 위를 향하는 엉겅퀴들과 ITS 염기서열은 유사하였다. 또한 정영엉겅퀴와 고려엉겅퀴는 형태학적으로는 구분이 거의 불가능하지만 ITS 염기서열에 기초한 분자유전학적 분석으로는 뚜렷이 다른 그룹임을 확인하였다. 채취된 엉겅퀴 및 도깨비엉겅퀴의 silymarin 생산 여부를 분석해 본 결과, silymarin이 공통적으로 존재하는 것으로 나타났다. 따라서 silymarin 생합성 능력은 Cirsium 속 식물들에서 공통적인 특징임을 알 수 있었다.

• Molecules and Cells
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• 제23권2호
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• pp.220-227
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• 2007

The development of suitable genetic markers would be useful for defining species and delineating the species boundaries of morphologically indistinguishable sponges. In this study, genetic variation in the sequences of nuclear rDNA and the mitochondrial cytochrome c oxidase subunit 1 and 3 (CO1 and CO3) regions were compared in morphologically indistinguishable Korean Halichondriidae sponges in order to determine the most suitable species-specific molecular marker region. The maximal congeneric nucleotide divergences of Halichondriidae sponges in CO1 and CO3 are similar to those found among anthozoan cnidarians, but they are 2- to 8-fold lower than those found among genera of other triploblastic metazoans. Ribosomal internal transcribed spacer regions (ITS: ITS1 + ITS2) showed higher congeneric variation (17.28% in ITS1 and 10.29% in ITS2) than those of CO1 and CO3. Use of the guidelines for species thresholds suggested in the recent literature indicates that the mtDNA regions are not appropriate for use as species-specific DNA markers for the Halichondriidae sponges, whereas the rDNA ITS regions are suitable because ITS exhibits a low level of intraspecific variation and a relatively high level of interspecific variation. In addition, to test the reliability of the ITS regions for identifying Halichondriidae sponges by PCR, a species-specific multiplex PCR primer set was developed.

• Animal Systematics, Evolution and Diversity
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• 제37권3호
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• pp.229-234
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• 2021

Scolelepis (Scolelepis) daphoinos is newly reported in Korean fauna. This species can be distinguished from its congeners by the following characteristics: the presence of reddish pigment patches on the posterior part of the prostomium, notopodial postchaetal lamellae that are partially fused to the branchiae, and the presence of only the bidentate hooded hooks. The morphological diagnosis and photographs of S. (S.) daphoinos are provided. The partial mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA(16S rDNA), and the nuclear 18S ribosomal DNA (18S rDNA) sequences from Korean specimens of S. (S.) daphoinos were determined. Species identification was supported by a comparison of DNA barcode sequences of COI and 16S rDNA with morphological examination from the specimens of type locality, China.

• 식물분류학회지
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• 제41권3호
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• pp.209-214
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• 2011

The taxonomy of the umbelliferous species Angelica amurensis and its allies was reviewed on the basis of molecular phylogenies derived from sequences of nuclear ribosomal DNA internal transcribed spacer (ITS) regions. Strict consensus of six minimal length 119-step trees derived from equally weighted maximum parsimony analysis of combined nuclear rDNA ITS1 and ITS2 sequences from 29 accessions of Angelica and outgroups indicated that Angelica purpuraefolia, known to be endemic to Korea, is the same species as A. amurensis. Comparisons of sequence pairs across both spacer regions revealed identity or 1-2 bp differences between A. purpuraefolia and A. amurensis. These results indicated that the two taxa are not distinguished taxonomically. Also, nuclear rDNA ITS regions are discussed as potential barcoding loci for identifying Korean Angelica.