• Title/Summary/Keyword: ITC

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Changes in Isothiocyanate Levels in Korean Chinese Cabbage Leaves during Kimchi Storage

  • Hong, Eun-Young;Kim, Gun-Hee
    • Food Science and Biotechnology
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    • v.15 no.5
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    • pp.688-693
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    • 2006
  • Glucosinolates are hydrolyzed by the enzyme myrosinase and are mainly found in cruciferous vegetables such as Chinese cabbage (Brassica campestris L. ssp. pekinensis). lsothiocyanates (ITCs) are glucosinolate degradation products with reported anticarcinogenic properties. Korean Chinese cabbage in the form of 'kimchi' is a staple part of the Korean diet. In this study, we examined the effects of storage temperature and duration on glucosinolate, ITC, soluble sugar, and organic acid levels in kimchi. Changes in pH and the impact of various parts of the Korean Chinese cabbage being used during the preparation of the dish were also assessed. Extracted ITC levels, analyzed via gas chromatography (GC) and GC/mass spectrometry (GC/MS), were higher in the midrib parts than in the cabbage leaves after storage at both 4 and $20^{\circ}C$. During storage, organic acid levels increased while soluble sugars were depleted. The pH initially increased (after 1 day at $20^{\circ}C$, and 1 week at $4^{\circ}C$), but subsequently decreased over time at both temperatures. Glucosinolate and ITC levels increased in the beginning of storage but then generally fell during further storage. Our data suggest that acidity-related reduction in myrosinase activity during storage may decrease glucosinolate and ITC levels. The changes in these levels depended on the storage conditions and the Korean Chinese cabbage parts used for the kimchi preparation.

Glucosinolates (3-Butenyl Isothiocyanate, Total Glucosinolates) Change in Seeds and Young Seedlings on Chinese Cabbages

  • Kim, Youn-Kyung;Hong, Eun-Young;Kim, Gun-Hee
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.04a
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    • pp.97.2-98
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    • 2003
  • The main objective of this study was to investigate the patterns of glucosinolates (3-butenyl isothiocyanate, total glucosinolates) in different parts of young seedling and seeds on Korean Chinese cabbages 55 days and Winter pride cultivars. For determination of glucosinolates, two cultivars of Chinese cabbages seeds and different parts of 1-day-old, 3-day-old, 5-day-old, and 7-day-old seedlings were used for analytical sample preparation provided with an anion exchanges column, and measured by GC and UV-Visible Spectrophotometer. 3-Butenyl ITC concentration was the highest in the cotyledon and the hypocotyl parts of 55 days cultivar seedling. In the cotyledon part of Winter pride cultivar seedling, 3-butenyl ITC amount was increase to 3-day-old seedling and then reduced. The cotyledon of 55 days cultivar contained the highest concentration of total glucosinolates while those were increased in the hypocotyl and decreased by degrees in the root. Total glucosinolates amounts were increased to 5-day-old seedling and then decreased in the cotyledon and the hypocotyl parts of seedling on Winter pride cultivar. There was no significant difference in the root part. In the seeds, both of 3-butenyl ITC and total glucosinolates, 55 days cultivar concentration was higher than Winter pride cultivar. The study has shown the variability in 3-butenyl ITC and total glucosinolates concentrations among cultivars, growth stages, and parts.

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Dopamine-dependent synaptic plasticity in an amygdala inhibitory circuit controls fear memory expression

  • Lee, Joo Han;Kim, Joung-Hun
    • BMB Reports
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    • v.49 no.1
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    • pp.1-2
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    • 2016
  • Of the numerous events that occur in daily life, we readily remember salient information, but do not retain most less-salient events for a prolonged period. Although some of the episodes contain putatively emotional aspects, the information with lower saliency is rarely stored in neural circuits via an unknown mechanism. We provided substantial evidence indicating that synaptic plasticity in the dorsal ITC of amygdala allows for selective storage of salient emotional experiences, while it deters less-salient experience from entering long-term memory. After activation of D4R or weak fear conditioning, STDP stimulation induces LTD in the LA-ITC synapses. This form of LTD is dependent upon presynaptic D4R, and is likely to result from enhancement of GABA release. Both optogenetic abrogation of LTD and ablation of D4R at the dorsal ITC in vivo lead to heightened and over-generalized fear responses. Finally, we demonstrated that LTD was impaired at the dorsal ITC of PTSD model mice, which suggests that maladaptation of GABAergic signaling and the resultant LTD impairment contribute to the endophenotypes of PTSD. [BMB Reports 2016; 49(1): 1-2]

Relationship between Chemical Structure and Antimicrobial Activities of Isothiocyanates from Cruciferous Vegetables against Oral Pathogens

  • Ko, Mi-Ok;Kim, Mi-Bo;Lim, Sang-Bin
    • Journal of Microbiology and Biotechnology
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    • v.26 no.12
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    • pp.2036-2042
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    • 2016
  • We evaluated the potentials of 10 isothiocyanates (ITCs) from cruciferous vegetables and radish root hydrolysate for inhibiting the growth of oral pathogens, with an emphasis on assessing any structure-function relationship. Structural differences in ITCs impacted their antimicrobial activities against oral pathogens differently. The indolyl ITC (indol-3-carbinol) was the most potent inhibitor of the growth of oral pathogens, followed by aromatic ITCs (benzyl ITC (BITC) and phenylethyl ITC (PEITC)) and aliphatic ITCs (erucin, iberin, and sulforaphene). Sulforaphene, which is similar in structure, but has one double bond, showed higher antimicrobial activity than sulforaphane. Erucin, which has a thiol group, showed higher antimicrobial activity than sulforaphane, which has a sulfinyl group. BITC and iberin with a short chain exhibited higher antimicrobial potential than PEITC and sulforaphane with a longer chain, respectively. ITCs have strong antimicrobial activities and may be useful in the prevention and management of dental caries.

Information for Urban Risk Management: the Role of Remote and Close Sensing

  • Hofstee, Paul;Genderen, John van
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.162-164
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    • 2003
  • The multi-disciplinary research project Strengthening Local Authorities in Risk Management (SLARIM), initiated by ITC, includes three case study cities in Asia. An important question is: what are the essential data for risk management and how to access such data. The role of common sources (e.g. census data), data derived from remote sensing (high-resolution satellite imagery, aerial photos), and data from close sensing (field observation, including mobile GIS) to acquire essential risk management data will be discussed. Special attention is given to the question of the minimum area and to disaggregating population data. A few examples are given of Kathmandu / Lalitpur, Nepal.

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Evaluation of Quality Characteristics of Broth Packets with Different Treatment of Dolsan Mustard Seeds (돌산갓 종자를 첨가한 국물용 육수팩의 품질특성)

  • Oh, SunKyung;Choi, MyeongRak
    • Journal of Life Science
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    • v.32 no.9
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    • pp.667-677
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    • 2022
  • Dolsan mustard seeds (DMS) were added in whole, crushed, and roasted form at 0.5 g (S-1), 1.0 g (S-2), and 1.5 g (S-3), respectively to broth and heated for 10 or 15 min. After cooling, the quality characteristics were measured. Salinity and pH decreased with boiling time. The antioxidant activities of the experimental broth were measured in terms of total polyphenol content, total flavonoid content, electron donating ability (EDA), 2,2-azino-bis (3-ethyl-benzothizoline-6-sulfonic acid) (ABTS) radical scavenging activity, and ferric reducing antioxidant power (FRAP). The overall, antioxidant activity was higher in broths containing 1.0 g and 1.5 g DMS than in those containing 0.5 g DMS and the activity increased with increasing boiling time. Sinigrin was not detected in the control group, and no significant difference in sinigrin content was noted among broths containing different concentrations of DMS. A high glutamic acid content was detected in the control broth, whereas glutamic acid, aspartic acid, glycine, proline, alanine, and arginine were detected in the broths containing DMS. The free amino acid contents, particularly aspartic acid and glutamic acid contents, were high in umami. Volatile components, such as 2-propenyl-isothiocyanate (ITC), allylthiocyanate, n-butyl ITC, and 3-butenyl ITC, were detected in the DMS-containing broths. Sensory evaluation revealed that a higher amount of DMS added and a longer heating time increased the overall taste preference, and the difference was statistically significant. The purpose of this study was to present basic data on the quality characteristics of DMS-added broths to aid in the development of new products using DMS.

Comparison of Common Enrichment Methods for Recovery of Yersinia Enterocolitica from Artificially Inoculated Swine Feed Samples

  • Kim, Joo-Sung;Draughon, F.A.
    • Journal of Food Hygiene and Safety
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    • v.25 no.4
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    • pp.320-324
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    • 2010
  • Five different enrichment methods were studied to find an optimal method to recover Yersinia enterocolitica from swine feed samples. When the recovery of Y. enterocolitica GER-C (serotype O:3) strain was studied at 1000 CFU/g feed, phosphate-buffered saline (PBS) enrichment at $4^{\circ}C$ and PBS plus sorbitol and bile salts (PSB) enrichment at $4^{\circ}C$ and $21^{\circ}C$ were not effective (< 22%). In contrast, both irgasan-ticarcillin-potassium chlorate (ITC) and tryptic soy broth plus polymyxin B sulfate and novobiocin (TSBPN) enrichment methods showed a full recovery (100%) at 100-1000 CFU/g feed. At 10 CFU/g feed, both ITC and TSBPN methods still recovered the strain (> 50%). In recovery of ATCC 9610 (serotype O:8) strain, TSBPN method was more sensitive than any other methods (P < 0.05) at 1000 CFU/g feed. Using TSBPN method, the strain was still recovered at 100 CFU/g feed, but not at 10 CFU/g feed. With its sensitivity and relatively simple recipe, TSBPN was most desirable method to recover Y. enterocolitica from swine feed samples.

Improvement of Hardwood Pulp Yield in Continuous Kraft Cooking and Estimation of Pulp Yields Pulp yields of isothermal cooking with polysulfide and anthraquinone

  • Ohi, Hiroshi;Yokoyama, Tomoya
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2006.06b
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    • pp.295-303
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    • 2006
  • The pulp yield was improved by about 4.5-5% when polysulfide (PS) and anthraquinone (AQ) were added to the kraft cooking liquor (white liquor). The exchange of the black liquor with fresh white liquor further increased the yield. The highest pulp yield was obtained when the PS cooking liquor containing 70% of total active alkali (AA) and 100% of AQ was used from the beginning of the reaction and the black liquor was exchanged with fresh white liquor containing the residual 30% of AA just after temperature reached $135^{\circ}C$. There was a good correlation between kraft pulp yields of a hardwood species and the ratios of the amount of xylose to glucose (X/G ratio), liberated by an acid hydrolysis of the pulps. However, the correlation was dependent on raw material wood species. Therefore, it is required in advance to establish a correlation between the yields and X/G ratios for raw material wood species of a target pulp in order to estimate pulp yield using X/G ratio. The X/G ratios of relatively high yield pulps showed higher values than those expected from the correlation. In a mill trial, the superiority of the PS-AQ isothermal cooking (ITC) process over the kraft ITC process was confirmed by examining X/G ratio of pulps obtained. The pulp yield in the PS-AQ ITC process was estimated at about 57.0%. This yield is very high, which indicates that reaction conditions of the PS-AQ ITC process are optimal.

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