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Comparison of Common Enrichment Methods for Recovery of Yersinia Enterocolitica from Artificially Inoculated Swine Feed Samples

Kim, Joo-Sung (The University of Tennessee, Food Safety Center of Excellence, Department of Food Science and Technology)
Draughon, F.A. (The University of Tennessee, Food Safety Center of Excellence, Department of Food Science and Technology)

Publication Information

Journal of Food Hygiene and Safety / v.25, no.4, 2010 , pp. 320-324 More about this Journal

Abstract

Five different enrichment methods were studied to find an optimal method to recover Yersinia enterocolitica from swine feed samples. When the recovery of Y. enterocolitica GER-C (serotype O:3) strain was studied at 1000 CFU/g feed, phosphate-buffered saline (PBS) enrichment at $4^{\circ}C$ and PBS plus sorbitol and bile salts (PSB) enrichment at $4^{\circ}C$ and $21^{\circ}C$ were not effective (< 22%). In contrast, both irgasan-ticarcillin-potassium chlorate (ITC) and tryptic soy broth plus polymyxin B sulfate and novobiocin (TSBPN) enrichment methods showed a full recovery (100%) at 100-1000 CFU/g feed. At 10 CFU/g feed, both ITC and TSBPN methods still recovered the strain (> 50%). In recovery of ATCC 9610 (serotype O:8) strain, TSBPN method was more sensitive than any other methods (P < 0.05) at 1000 CFU/g feed. Using TSBPN method, the strain was still recovered at 100 CFU/g feed, but not at 10 CFU/g feed. With its sensitivity and relatively simple recipe, TSBPN was most desirable method to recover Y. enterocolitica from swine feed samples.

Keywords

Yersinia enterocolitica; enrichment; swine; feed;

Citations & Related Records

Reference

1	Tajkarimi, M., Riemann, H.P., Hajmeer, M.N., Gomez, E.L., Razavilar, V., Cliver, D.O.: Ammonia disinfection of animal feeds-laboratory study. Int. J. Food Microbiol., 122, 23-28 (2008). DOI ScienceOn
2	Gurtler, M., Alter, T., Kasimir, S., Linnebur, M., Fehlhaber, K.: Prevalence of Yersinia enterocolitica in fattening pigs. J. Food Prot., 68, 850-854 (2005).
3	Kwaga, J., Iversen, J.O., Saunders, J.R.: Comparison of two enrichment protocols for the detection of Yersinia in slaughtered pigs and pork products. J. Food Prot., 53, 1047-1049 (1990).
4	Crump, J.A., Griffin, P.M., Angulo, F.J.: Bacterial contamination of animal feed and its relationship to human foodborne illness. Clin. Infect. Dis., 35, 859-865 (2002). DOI ScienceOn
5	Rho, M.J., Chung, M.S., Lee, J.H., Park, J.: Monitoring of microbial hazards at farms, slaughterhouses, and processing lines of swine in Korea. J. Food Prot., 64, 1388-1391 (2001).
6	Laukkanen, R., MartÌnez, P.O., Siekkinen, K.M., Ranta, J., Maijala, R., Korkeala, H.: Contamination of carcasses with human pathogenic Yersinia enterocolitica 4/OT : 3 originates from pigs infected on farms. Foodborne Pathog. Dis., 6, 681- 688 (2009). DOI ScienceOn
7	Wauters, G., Goossens, V., Janssens, M., Vandepitte, J.: New enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup O:3 from pork. Appl. Environ. Microbiol., 54, 851-854 (1988).
8	Bowman, A.S., Glendening, C., Wittum, T.E., LeJeune, J.T., Stich, R.W., Funk, J.A.: Prevalence of Yersinia enterocolitica in different phases of production on swine farms. J. Food Prot., 70, 11-16 (2007).
9	Funk, J.A., Troutt, H.F., Isaacson, R.E., Fossler, C.P.: Prevalence of pathogenic Yersinia enterocolitica in groups of swine at slaughter. J. Food Prot., 61, 677-682 (1998).
10	Fredriksson-Ahomaa, M., Stolle, A., Korkeala, H.: Molecular epidemiology of Yersinia enterocolitica infections. FEMS Immunol. Med. Microbiol., 47, 315-329 (2006). DOI ScienceOn
11	Sandery, M., Stinear, T., Kaucner, C.: Detection of pathogenic Yersinia enterocolitica in environmental waters by PCR. J. Appl. Bacteriol., 80, 327-332 (1996). DOI
12	Boyapalle, S., Wesley, I.V., Hurd, H.S., Reddy, P.G.: Comparison of culture, multiplex, and 5' nuclease polymerase chain reaction assays for the rapid detection of Yersinia enterocolitica in swine and pork products. J. Food Prot., 64, 1352-1361 (2001).
13	Fredriksson-Ahomaa, M., Hielm, S., Korkeala, H.: High prevalence of yadA-positive Yersinia enterocolitica in pig tongues and minced meat at the retail level in Finland. J. Food Prot., 62, 123-127 (1999).
14	Johannessen, G.S., Kapperud, G., Kruse, H.: Occurrence of pathogenic Yersinia enterocolitica in Norwegian pork products determined by a PCR method and a traditional culturing method. Int. J. Food Microbiol., 54, 75-80 (2000). DOI
15	Mehlman, I.J., Aulisio, C.C.G., Sanders, A.C.: Problems in the recovery and identification of Yersinia from food. J. Assoc. Off. Anal. Chem., 61, 761-771 (1978).
16	Wesley, I.V., Bhaduri, S., Bush, E.: Prevalence of Yersinia enterocolitica in market weight hogs in the United States. J. Food Prot., 71, 1162-1168 (2008).
17	de Boer, E., Nouws, J.F.: Slaughter pigs and pork as a source of human pathogenic Yersinia enterocolitica. Int. J. Food Microbiol., 12, 375-378 (1991). DOI ScienceOn
18	Fredriksson-Ahomaa, M., Stolle, A., Stephan, R.: Prevalence of pathogenic Yersinia enterocolitica in pigs slaughtered at a Swiss abattoir. Int. J. Food Microbiol., 119, 207-212 (2007). DOI ScienceOn
19	Landgraf, M., Iaria, S.T., Falcão, D.P.: An improved enrichment procedure for the isolation of Yersinia enterocolitica and related species from milk. J. Food Prot., 56, 447-450 (1993).
20	Bonardi, S., Brindani, F., Pizzin, G., Lucidi, L., D'Incau, M., Liebana, E., Morabito, S.: Detection of Salmonella spp., Yersinia enterocolitica and verocytotoxin-producing Escherichia coli O157 in pigs at slaughter in Italy. Int. J. Food Microbiol., 85, 101-110 (2003). DOI
21	Pilon, J., Higgins, R., Quessy, S.: Epidemiological study of Yersinia enterocolitica in swine herds in Quebec. Can. Vet. J., 41, 383-387 (2000).
22	Doane, C.A., Pangloli, P., Richards, H.A., Mount, J.R., Golden, D.A., Draughon, F.A.: Occurrence of Escherichia coli O157: H7 in diverse farm environments. J. Food Prot., 70, 6-10 (2007).
23	Pangloli, P., Dje, Y., Ahmed, O., Doane, C.A., Oliver, S.P., Draughon, F.A.: Seasonal incidence and molecular characterization of Salmonella from dairy cows, calves, and farm environment. Foodborne. Pathog. Dis., 5, 87-96 (2008). DOI ScienceOn
24	Letellier, A., Messier, S., Quessy, S.: Prevalence of Salmonella spp. and Yersinia enterocolitica in finishing swine at Canadian abattoirs. J. Food Prot., 62, 22-25 (1999).
25	Nesbakken, T., Gondrosen, B., Kapperud, G.: Investigation of Yersinia enterocolitica, Yersinia enterocolitica-like bacteria, and thermotolerant campylobacters in Norwegian pork products. Int. J. Food Microbiol., 1, 311-320 (1985). DOI ScienceOn
26	Bhaduri, S., Wesley, I.V., Bush, E.J.: Prevalence of pathogenic Yersinia enterocolitica strains in pigs in the United States. Appl. Environ. Microbiol., 71, 7117-7121 (2005). DOI ScienceOn
27	Fredriksson-Ahomaa, M., Korkeala, H.: Low occurrence of pathogenic Yersinia enterocolitica in clinical, food, and environmental samples: a methodological problem. Clin. Microbiol. Rev., 16, 220-229 (2003). DOI ScienceOn
28	Schiemann, D.A.: Yersinia enterocolitica and Yersinia pseudotuberculosis. In Foodborne bacterial pathogens, (Doyle, M.P. ed.) M. Dekker, New York, pp. 602-640 (1989).