• 한국기계가공학회지
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• 제18권2호
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• pp.44-51
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• 2019

With the goal of tissue regeneration for organs damaged through an accident or a disease, research on tissue engineering has been conducted to produce 3-D scaffolds that can support the cells in the attachment and growth for the cell proliferation and differentiation. A scaffold requires a suitable pore size and porosity to increase the nutrient circulation or oxygen supply for the attachment and growth of cells. The existing production methods such as solvent-casting particulate leaching, phase separation, and fiber bonding have certain disadvantages. With these methods, it is difficult to obtain a free desired shape. In addition, certain pore sizes and interconnectivities among the pores may not be guaranteed. To solve these problems, this study has fabricated a scaffold with a 3-D shaped nose using Alginate, which is a natural polymer obtained through Fused Deposition Modeling (FDM), one of the CAD/CAM-based Solid Freeform Fabrication (SFF) methods.

• IMMUNE NETWORK
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• 제22권5호
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• pp.38.1-38.24
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• 2022

Exosomes, which are well-known nanoscale extracellular vesicles, are multifunctional biomaterials derived from endosomes and perform various functions. The exosome is a critical material in cell-cell communication. In addition, it regulates the pathophysiological conditions of the tumor microenvironment in particular. In the tumor microenvironment, exosomes play a controversial role in supporting or killing cancer by conveying biomaterials derived from parent cells. Innate immunity is a crucial component of the host defense mechanism, as it prevents foreign substances, such as viruses and other microbes and tumorigenesis from invading the body. Early in the tumorigenesis process, the innate immunity explicitly recognizes the tumor via Ags and educates the adaptive immunity to eliminate it. Recent studies have revealed that exosomes regulate immunity in the tumor microenvironment. Tumor-derived exosomes regulate immunity against tumor progression and metastasis. Furthermore, tumor-derived exosomes regulate polarization, differentiation, proliferation, and activation of innate immune cells. Exosomes produced from innate immune cells can inhibit or support tumor progression and metastasis via immune cell activation and direct cancer inhibition. In this study, we investigated current knowledge regarding the communication between tumor-derived exosomes and innate immune cell-derived exosomes (from macrophages, dendritic cells, NK cells, and neutrophils) in the tumor microenvironment. In addition, we discussed the potential development of exosomal immunotherapy using native or engineered exosomes against cancer.

• 임상간호연구
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• 제18권1호
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• pp.126-135
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• 2012

Purpose: This study was aimed to develop a simulation training program of an advanced cardiac life support (ACLS) based on the mastery learning model (Simulation-MLM), and evaluate the effects of the program on critical care nurses. Methods: As an experimental pre-post test with a non-equivalent control group, the study employed convenience sampling of 38 critical care nurses. The experimental group received the Simulation-MLM including a theoretical lecture, formative evaluation, and simulation training, whereas only a theoretical lecture for the control group. The knowledge, self-efficacy, and performance degrees of respondents were measured to verify the effects of the Simulation-MLM. The statistical processing of the collected data utilized the SPSS WIN 17.0 program. Results: After receiving Simulation-MLM, the participants in the experimental group reported higher marks in the knowledge, self-efficacy and performance of ACLS compared with those in the control group. However, both experimental and control groups demonstrated no significant differences in knowledge, self-efficacy and performance. Conclusion: Despite of the limitation of a small sample size, this study was considered meaningful in a sense that it showed a venue for improving ACLS training efficiency. Future research with more distinct treatment differentiation and better adequate outcome variables was warranted in order to prove the effects of a theory-based simulation education.

• Biomaterials and Biomechanics in Bioengineering
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• 제2권3호
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• pp.143-157
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• 2015

Successful integration of cementless femoral stems using porous surfaces relies on effective periimplant bone healing to secure the bone-implant interface. The initial stages of the healing process involve protein adsorption, fibrin clot formation and cell osteoconduction onto the implant surface. Modelling this process in vitro, the current work considered the effect of fibrin deposition on the responses of human mesenchymal stromal cells cultured on ferritic fibre networks intended for magneto-mechanical actuation of in-growing bone tissue. The underlying hypothesis for the study was that fibrin deposition would support early stromal cell attachment and physiological functions within the optimal regions for strain transmission to the cells in the fibre networks. Highly porous fibre networks composed of 444 ferritic stainless steel were selected due to their ability to support human osteoblasts and mesenchymal stromal cells without inducing untoward inflammatory responses in vitro. Cell attachment, proliferation, metabolic activity, differentiation and penetration into the ferritic fibre networks were examined for one week. For all fibrin-containing samples, cells were observed on and between the metal fibres, supported by the deposited fibrin, while cells on fibrin-free fibre networks (control surface) attached only onto fibre surfaces and junctions. Initial cell attachment, measured by analysis of deoxyribonucleic acid, increased significantly with increasing fibrinogen concentration within the physiological range. Despite higher cell numbers on fibrin-containing samples, similar metabolic activities to control surfaces were observed, which significantly increased for all samples over the duration of the study. It is concluded that fibrin deposition can support the early attachment of viable mesenchymal stromal cells within the inter-fibre spaces of fibre networks intended for magneto-mechanical strain transduction to in-growing cells.

• 대한지리학회지
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• 제43권3호
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• pp.337-357
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• 2008

본 논문은 서울의 인구고령화 현상을 살펴보고 이들 고령자 취업의 지역적 특성을 고찰하는 연구이다. 서울시 구별 자료를 토대로 노령화지수와 장수도를 산출하여 시 공간적 변화와 차이를 확인하였다. 또한 고령자취업알선센터의 자료를 통해 구인처와 구직자, 취업자에 대한 분석을 시도하였다 그 결과, 서울의 인구고령화는 다른 지역에 비해 최근 들어서 빠른 속도로 진행되었고, 서울 내에서 각 구별로 지역적 차별화를 보였다. 고령자의 취업현황을 살펴보면 단순노무직 비율이 전국 평균에 비해 높게 나타나고, 고용의 지역적 차별화로 인해, 몇몇 지역을 제외하고는 고령자를 위한 고용기회가 충분하게 주어지지 않았다. 결과적으로, 구인처와 구직자, 취업자간에는 지리적 부조응(geographical mismatch)이 나타났는데, 이는 고령구직자가 자신의 주거지 주위에서 적절한 일자리를 얻기 힘들다는 것을 의미하며, 고령자의 낮은 이동성을 고려할 때 정책적 제도적 지원 및 개선이 필요하다고 생각된다.

• 한국사회정책
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• 제25권2호
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• pp.367-402
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• 2018

본 연구는 발달장애인 권리보장 및 지원에 관한 법률과 이를 모법으로 하는 지방자치단체의 유사 조례간 연계성(일치성) 및 자치성(차별성)을 분석하였다. 2017년 10월 기준으로 조회된 전국 63개의 지자체 조례와 발달장애인법을 분석 대상으로 하여 모법의 조항별 내용을 기준으로 조례와의 일치성을 분석하였고 자치성은 조례의 내용적 측면에서 모법과 다른 사례가 있는지 행정적 측면에서 이행 의지를 보다 강조한 사례가 있는지 내용분석으로 시행하였다. 분석 결과 첫째, 모법의 조례 반영률은 조항별로 다르게 나타났는데, 복지지원을 강조하는 측면에서 일치율이 높았고 발달장애인의 권리 보장은 조례상 반영률이 낮았다. 이는 발달장애인을 보호적 관점에서 보는 법적 제도적 특징을 보이는 증거로 볼 수 있다. 향후에는 발달장애인 당사자의 의사결정에 대한 존중이나 권리보장에 관한 내용이 조례에 적극적으로 포함될 수 있도록 하는 정책방안이 필요하다. 둘째, 조례 반영률이 0%인 조항도 있었는데, 해당 지역에서 타법에 의해 보장될 수도 있기에 지역 내 관련 제도의 부재를 의미하지는 않으나 제도적 사각지대의 가능성이 있다. 향후에는 발달장애인 관련 지역 내 여타 법제도와의 상보성에 관한 검토가 이루어져 제도적 사각지대에 발달장애인이 놓이지 않도록 해야 할 것이다. 셋째, 지역의 자치성이 내용적 측면 및 실질적 이행을 돕는 행정적 측면에서 검토되었다. 단체장의 책무를 강조하거나 실태조사를 강조하거나 복지위원회 운영을 명시하거나 모법상에 없는 내용을 추가적으로 조례에서 담아내고 있기도 했다. 향후 조례제정을 고려하는 지자체는 이와 같은 사례를 참고하여 지방자치의 특성을 살린 지역 밀착적인 실현가능한 조례를 제정할 필요가 있다.

• Tissue Engineering and Regenerative Medicine
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• 제15권6호
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• pp.673-697
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• 2018

BACKGROUND: Cartilage tissue engineering (CTE) aims to obtain a structure mimicking native cartilage tissue through the combination of relevant cells, three-dimensional scaffolds, and extraneous signals. Implantation of 'matured' constructs is thus expected to provide solution for treating large injury of articular cartilage. Type I collagen is widely used as scaffolds for CTE products undergoing clinical trial, owing to its ubiquitous biocompatibility and vast clinical approval. However, the long-term performance of pure type I collagen scaffolds would suffer from its limited chondrogenic capacity and inferior mechanical properties. This paper aims to provide insights necessary for advancing type I collagen scaffolds in the CTE applications. METHODS: Initially, the interactions of type I/II collagen with CTE-relevant cells [i.e., articular chondrocytes (ACs) and mesenchymal stem cells (MSCs)] are discussed. Next, the physical features and chemical composition of the scaffolds crucial to support chondrogenic activities of AC and MSC are highlighted. Attempts to optimize the collagen scaffolds by blending with natural/synthetic polymers are described. Hybrid strategy in which collagen and structural polymers are combined in non-blending manner is detailed. RESULTS: Type I collagen is sufficient to support cellular activities of ACs and MSCs; however it shows limited chondrogenic performance than type II collagen. Nonetheless, type I collagen is the clinically feasible option since type II collagen shows arthritogenic potency. Physical features of scaffolds such as internal structure, pore size, stiffness, etc. are shown to be crucial in influencing the differentiation fate and secreting extracellular matrixes from ACs and MSCs. Collagen can be blended with native or synthetic polymer to improve the mechanical and bioactivities of final composites. However, the versatility of blending strategy is limited due to denaturation of type I collagen at harsh processing condition. Hybrid strategy is successful in maximizing bioactivity of collagen scaffolds and mechanical robustness of structural polymer. CONCLUSION: Considering the previous improvements of physical and compositional properties of collagen scaffolds and recent manufacturing developments of structural polymer, it is concluded that hybrid strategy is a promising approach to advance further collagen-based scaffolds in CTE.

• 한국컴퓨터산업학회논문지
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• 제2권12호
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• pp.1505-1516
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• 2001

The goal of Medical multimedia server is to develop computer hardware and software which would enable electronic access, storage, transmission, and display of patient data and images. Since the current network only provides so called "best-effort" services, it is impossible to satisfy QoS guarantee that is required for real time application services for emergency room, operating room etc. Accordingly, world-wide research is being made for a variety of services to provide QoS. he goal of DiffServ is to offer scalable differentiated service in Internet which are made possible by traffic classification and conditioning only performed at an edge(or a boundary) node. In case DiffServ was deployed in the Medical multimedia network, it is difficult to estimate how the QoS mechanism would affect totally the network performance. Therefore, we need to verify by simulation the design of algorithm which provide a variety of differentiated services. In QoS for Medical multimedia network, a simulator is designed and implemented using OPNET to investigate the performance of DiffServ QoS support mechanism. The developed DiffServ simulator may generate packets according to random, and bursty traffic models in order to incorporate diverse traffic conditions in the Medical multimedia network environment. Based on our simulation results, we confirmed that service differentiation is possible by using the EF(Expedited Forwarding) class in DiffServ networks.

• Journal of Periodontal and Implant Science
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• 제32권4호
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• pp.733-744
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• 2002

The fibroblasts are the principal cells in the periodontal ligament of peridontium. As the periodontal ligament fibroblasts (PDLF) show similar phenotype with osteoblasts, the PDLF are thought to play an important role in alveolar bone remodeling. Cell-to-cell contacted signaling is crucial for osteoclast formation. Recently it has been reported that PDLJ enhance the bone resorbing activity of osteoclasts differentiated from hematopoietic preosteoclasts. The aims of this study were to $clarify\;^{1)}$ the mechanism of PDLF-induced osteoclastogenesis $and\;^{2)}$ whether we can use preosteoclast cell line instead of primary hematopoietic preosteoclast cells for studying the mechanism of PDLF-induced osteoclastogenesis. Osteoclastic differentiation of mouse macrophage cell line RAW264.7 was compared with that of mouse bone marrow-derived M-CSF dependent cell (MDBM), a well-known hematopoietic preosteoclast model, by examining, 1) osteoclast-specific gene expression such as calcitonin receptor, M-CSF receptor (c-fms), cathepsin K, receptoractivator nuclear factor kappa B (RANK) ,2) generation of TRAP(+) multinucleated cells (MNCs), and 3) generation of resorption pit on the $OAAS^{TM}$ plate. RAW264.7 cultured in the medium containing of soluble osteoclast differentiation Factor (sODF) showed similar phenotype with MDBM-derived osteoclasts, those are mRNA expression pattern of osteoclast-specific genes, TRAP(+) MNCs generation, and bone resorbing abivity. Formation of resorption pits by osteoclastic MNCs differentiated from sODF-treated RAW264.7, was completely blocked by the addition of osteoprotegerin (OPG), a soluble decoy receptor for ODF, to the sODF-containing culture me야um. The effects of PDLF on differentiation of RAW264.7 into　the TRAP(+) multinucleated osteoclast-like cells were examined using coculture system. PDLF were fxed with paraformaldehyde, followed by coculture with RAW264.7, which induced formation of TRAP(+) MNCs in the absence of additional treatment of sODF. When compared with untreated and fixed PDLF (fPDLF), IL-1 ${\beta}$-treated, or lipopolysaccha-ride-treated and then fixed PDLF showed two-folld increase in the supporting activity of osteoclastogenesis from RAW264.7 coculture system. There were no TRAP(+) MNCs formation in coculture system of RAW264.7 with PDLF of no fixation. These findigs suggested that we can replace the primary hematopoietic preosteoclasts for RAW264. 7 cell line for studying the mechanism of PDLF-induced osteoclastogenesis, and we hypothesize that PDLF control osteoclastogenesis through ODF expression which might be enhanced by inflammatory signals.

• 지능정보연구
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• 제20권3호
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• pp.151-171
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• 2014

SaaS는 사용자가 필요한 소프트웨어를 인터넷을 통해 원격으로 서비스 받을 수 있도록 하는 모델로 소프트웨어 시장에서 차지하는 비중이 커짐과 동시에 관련 분야의 비즈니스 요구사항의 증가에 따라 지속적인 성장이 기대되는 분야이다. 이에 본 연구는 SaaS 공급업체들을 대상으로 기업에서 추구하는 차별화 전략 및 낮은 가격전략과 고객획득성과와의 관계를 살펴보고 더 나아가 이들 간의 관계에서 SaaS 기술성숙도 수준의 매개효과와 조절효과를 알아보고자 하였다. 이를 위해 SaaS 제공업체 및 국내 CNK(commerce net Korea) 데이터베이스에 등록된 업체의 어플리케이션을 대상으로, 175개 기업 총 199개 SaaS 전략사업단위의 설문결과를 분석에 활용하였다. SaaS 기술성숙도가 차별화전략 및 낮은가격전략과 고객획득성과와의 관계를 매개하는지 검증하기 위해 Baron and Kenny (1986)가 제안한 절차에 따라 회귀분석을 실시하였고, SaaS 기술성숙도의 조절효과를 살펴보기 위해 위계적 회귀분석(hierarchical regression analysis) 방법을 적용한 상호작용효과를 검증하였다. 분석결과, 첫째, SaaS 제공업체가 추구하는 차별화 전략(업종특화, 파트너활용, 전담인력수) 및 낮은 가격전략(월이용료, 초기설치비)과 같은 기업전략은 고객획득에 긍정적인 영향을 미치는 것으로 나타났다. 또한, SaaS 공급업체의 기술성숙도 수준(어플리케이션 서비스 제공, 웹 기본 어플리케이션, 웹 서비스 어플리케이션)과 고객 획득성과 간에 유의미한 긍정적인 관계가 있는 것으로 확인되었다. 마지막으로, SaaS 기술성숙도 수준의 기업전략과 고객획득성과와의 관계에 대한 조절효과는 주로 차별화 전략에 대해 나타난 반면, 매개효과는 주로 낮은 가격전략에 대해 나타남을 확인하였다.