• Title/Summary/Keyword: ISU

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Development of an Enzyme-Linked Immunosorbent Assay Method for Residual Host Cell derived Proteins in Recombinant Antibody Drug Production (재조합 항체의약품의 생산시 생산세포주 유래 단백질 검출을 위한 ELISA 방법 개발)

  • Joung, Chan-Hi;Lim, Sang-Min;Koo, Yoon-Mo;Lee, Yong-Yoon;Son, Young-Su;Kim, Hyun-Il;Park, Heung-Rok
    • KSBB Journal
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    • v.21 no.3
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    • pp.212-219
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    • 2006
  • The purpose of this study was to develop a assay system of host cell-derived residual proteins in final pharmaceutical products. Accurate and simple assay system for host cell-derived proteins(HCPs) is very important test item in pharmaceutical qualification control. In this study, methods for quantification of residual HCPs in recombinant anti-GPIIbIIIa antibody were developed using a process-specific immunoligand assay which was based on the Enzyme linked Immunosorbent assay(ELISA) system. The assay had a detection limit of 10.8 ng/ml of HCPs with a product concentration of 1 mg/ml. The practical implication of these results is that the developed ELISA system can be used for HCPs qualification control and this system will be applicable to develop another ELISA system of different antibody drug.

Laboratory investigation of the effects of translation on the near-ground tornado flow field

  • Razavi, Alireza;Sarkar, Partha P.
    • Wind and Structures
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    • v.26 no.3
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    • pp.179-190
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    • 2018
  • Translation of tornadoes is an important feature in replicating the near-ground tornado flow field which has been simulated in previous studies based on Ward-type tornado simulators using relative motion of the ground plane. In this laboratory investigation, effects of translation on the near-ground tornado flow field were studied using the ISU Tornado Simulator that can physically translate over a ground plane. Two translation speeds, 0.15 m/s and 0.50 m/s, that scale up to those corresponding to slowly-moving tornadoes in the field were selected for this study. Compared with the flow field of a stationary tornado, the simulated tornado with translation had an influence on the spatial distribution and magnitude of the horizontal velocities, early reversal of the radial inflow, and expansion of the core radius. Maximum horizontal velocities were observed to occur behind the center of the translating tornado and on the right side of its mean path. An increase in translation speed, resulted in reduction of maximum horizontal velocities at all heights. Comparison of the results with previous studies that used relative motion of the ground plane for simulating translating tornadoes, showed that translation has similar effects on the flow field at smaller radial distances (~2 core radius), but different effects at larger radial distances (~4 core radius). Further, it showed that the effect of translation on velocity profiles is noticeable at and above an elevation of ~0.6 core radius, unlike those in studies based on the relative motion of the ground plane.

The Activity Information Strategies of Elementary students groups (초등학교 청소년 단체의 활동 정보화 방안)

  • Seo, Hye-Joung;Hong, Myung-Hui
    • 한국정보교육학회:학술대회논문집
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    • 2004.08a
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    • pp.428-437
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    • 2004
  • 정보화 사회로의 변화는 교육부문에 있어서도 새로운 패러다임를 요구하며, 학교 학생 학부모의 교육정보 공유와 시공간을 초월한 학교교육은 더욱 강조되고 있다. 전인적 교육을 이루어주는 청소년단체는 그 중요성과 규모는 날로 커지고 있으나 교육목표 달성에의 어려움은 더욱 늘어가고, 정보화는 거의 이루어지지 못한 영역이다. 이에 여러 학교의 청소년단체의 실태를 살펴 관련 정보를 분석하고 정보화 방안을 연구하여 청소년단체의 교육목표 달성을 돕는 지원시스템을 설계해보고자 하였다.

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The theory of educational usage with wordprocessor Based Mindtool (워드프로세서 기반 마인드툴의 교육적 활용방안)

  • Seo, Hye-Joung;Hong, Myung-Hui
    • 한국정보교육학회:학술대회논문집
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    • 2004.01a
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    • pp.393-402
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    • 2004
  • 마인드툴은 의미 있는 학습을 이루고 학습자의 사고과정을 도와주어 비판적 사고력, 창의적 사고력, 종합적 사고력을 가지도록 해준다. 워드프로세서의 다이어그램, 조직도, 그리기도구모음, 표, 워크시트, 차트, 하이퍼미디어 기능을 분석하여 마인드툴의 유의미조직도구, 동적모델링 도구, 학습내용 구성 도구로 활용하는 방법을 모색해 보았다.

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Development of an active gust generation mechanism on a wind tunnel for wind engineering and industrial aerodynamics applications

  • Haan, Fred L. Jr.;Sarkar, Partha P.;Spencer-Berger, Nicholas J.
    • Wind and Structures
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    • v.9 no.5
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    • pp.369-386
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    • 2006
  • A combination Aerodynamic/Atmospheric Boundary Layer (AABL) Wind and Gust Tunnel with a unique active gust generation capability has been developed for wind engineering and industrial aerodynamics applications. This facility is a cornerstone component of the Wind Simulation and Testing (WiST) Laboratory of the Department of Aerospace Engineering at Iowa State University (ISU). The AABL Wind and Gust tunnel is primarily a closed-circuit tunnel that can be also operated in open-return mode. It is designed to accommodate two test sections ($2.44m{\times}1.83m$ and $2.44m{\times}2.21m$) with a maximum wind speed capability of 53 m/s. The gust generator is capable of producing non-stationary gust magnitudes around 27% of the mean flow speed. This paper describes the motivation for developing this gust generator and the work related to its design and testing.

Analysis of Success Factors for Mobile Commerce using Text Mining and PLS Regression

  • Kim, Yong-Hwan;Kim, Ja-Hee;Park, Ji hoon;Lee, Seung-Jun
    • Journal of the Korea Society of Computer and Information
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    • v.21 no.11
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    • pp.127-134
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    • 2016
  • In this paper, we propose factors that influence on the mobile commerce satisfaction conducted by data mining and a PLS regression analysis. We extracted the most frequent words from mobile application reviews in which there are a large number of user's requests. We employed the content analysis to condense the large number of texts. We took a survey with the categories by which data are condensed and specified as factors that influence on the mobile commerce satisfaction. To avoid multicollinearity, we employed a PLS regression analysis instead of using a multiple regression analysis. Discovered factors that are potential consequences of customer satisfaction from direct requests by customers, the result may be an appropriate indicator for the mobile commerce market to improve its services.

Conducting Polypyrrole Doped with Hexacyanoferrate Anions: an Electrochemical and Spectroscopic Study

  • Han Junghee;Lee Seungjun;Paik Woon-kie
    • Bulletin of the Korean Chemical Society
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    • v.13 no.4
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    • pp.419-425
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    • 1992
  • Conducting polypyrrole doped with iron (Ⅱ,Ⅲ) hexacyanate Fe$(CN)_6^{z-}$ ions was studied for its physical and electrochemical properties. The polymer exhibited two pairs of waves in the cyclic voltammogram, one for the reversible oxidation/reduction of the incorporated iron hexacyanate ions and the other for the near-reversible oxidation/reduction of the polypyrrole moiety. The exchange of ions incorporated in the polymer and other ions present in solutions were examined by following the decrease of the reversible redox peaks of Fe$(CN)_6^{z-}$, and by EDX analysis. The spin density of this highly conducting polymer as probed by ESR spectroscopy was extremely low compared to polypyrrole doped with common anions.

Voice-based Control System Using Standard-based IoT Platforms (표준 사물인터넷 플랫폼을 활용한 음성 제어 시스템)

  • Jeong, Isu;Baek, Seungwoo;Lee, Sungchan;Yun, Jaeseok
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2019.01a
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    • pp.454-455
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    • 2019
  • 본 논문에서는 표준 기반 사물인터넷 (IoT: Internet of Things) 플랫폼을 활용한 음성 제어 시스템을 구현하고 그 성능을 검증한다. 사물인터넷 산업 표준인 원엠투엠 (oneM2M) 오픈 소스 플랫폼을 활용하여 음성으로 댁내 기기를 제어할 수 있는 프로토타입 시스템을 구현하였다. 음성 기반 제어를 위해 구글의 Speech-to-Text API를 활용하고 오픈 소스 하드웨어에 원엠투엠 플랫폼을 탑재하여 어디서든지 서버 플랫폼에 연결된 댁내 가전기기들을 제어할 수 있음을 보였다. 본 논문에서 구현한 시스템을 통해 표준화된 오픈 소스 플랫폼과 클라우드 음성 인식 API를 활용하여 확장성과 연결성을 갖춘 커넥티드 홈을 구현할 수 있음을 알 수 있다.

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