• KOREAN JOURNAL OF CROP SCIENCE
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• v.47
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• pp.200-215
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• 2002

There are some international standards and organization controling the quality characteristics of varieties and seeds such as UPOV, OECD, ISTA, AOSA. The National List system to check variety performance such as quality characteristics of plant varieties and seeds was established by each countries. The UPOV (International Union for the Protection of New varieties of Plant) regulates the requirement of plant variety protection that is distinctness (D), uniformity (U), stability (S) (here in after called DUS), Novelty and Denomination, and some quality characteristics are used to examine the requirement of plant variety protection by each crops. OECD (Organization for Economic Cooperation Development) seed scheme regulates the seed certification for seed trade between countries. ISTA (International Seed Testing Association) and AOSA(Association for Official Seed Analyst) regulates the seed analysis standard and methods. Most of the countries in Europe has the National List system, which check the value for cultivation and use(VCU), that is to say, variety performance such as yield, quality and stress to environment. All the seeds should be enlisted in the National List before sell the seed in their country. All the quality characteristics checked variety performance are for instance, amylose and amylopectin content in rice, molting quality, $\beta$-glucan, protein in barley, protein and sugar content in soybean, sugar and amylopectin content in corn etc. Conditions for the protection of new variety of plant are DUS. Quality characteristics may be the important characteristics and used to check DUS in crop by crop. It is very important to develop a new characteristics and establish standard method fur examine the VCU and DUS test for each crop.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.3
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• pp.23-38
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• 2015

The study was initiated with Kentucky bluegrass (KB, Poa pratensis L.) to investigate germination and early establishment characteristics of new cultivars for a practical application to turfgrass establishment such as parks, athletic field and golf course etc. Fifteen cultivars were evaluated in different experiments. An alternative condition for a KB germination test required by International Seed Testing Association (ISTA) was applied in Experiment I, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Experiment II was conducted under a room temperature condition of 5 to $25^{\circ}C$ (natural conditions). Seed germinating vigor, germination speed and germination peak time were measured in both experiments. Significant differences were observed in seed germinating vigor, germination speed and germination peak time. Seed germinating vigor was variable with different environments and cultivars. It was 75.25 to 89.50% under ISTA conditions and 75.75 to 90.25% under natural conditions. There were considerable variations in early germination characteristics among KB cultivars according to different environments. Early germination characteristics showed that all cultivars were 3 to 5 days faster in germination under ISTA conditions, when compared with natural conditions. The germination speed, measured as days to seed germination of 50% to 80%, was much faster with 'Midnight II', 'Excursion' and 'Midnight' under ISTA conditions. But it was faster with 'Midnight II', 'Excursion' and 'Odyssey' under natural conditions. Differences was also observed in germination peak time with cultivars and growing conditions. It ranged 5.94 to 14.88 days under ISTA conditions and 4.71 to 13.06 days under natural conditions. Regardless of the environment conditions, the shortest cultivars were 'Odyssey' and 'Midnight II'. The longest ones were 'Nuglade' under ISTA condition and 'Moonlight' under natural conditions. Considering germinating vigor, germination speed and germination peak time, 'Midnight II', 'Excursion', 'Midnight', and 'Odyssey' were regarded as excellent cultivars under ISTA conditions in terms of early establishment characteristics, while 'Midnight II', 'Excursion', 'Odyssey', and 'Courtyard' under natural conditions. These results suggest that an intensive germination test be needed prior to planting, for investigating the early germinating vigor, germination speed and germination peak time. Also, a proper cultivar selection and comprehensive site analysis for the growing environmental conditions should be done before turfgrass establishment such as golf course construction.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.5
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• pp.79-91
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• 2008

Research was initiated to investigate germination characteristics of creeping bentgrass (CB, Agrostis palustris Huds.). Seven varieties of CB were evaluated with different experiments. An alternative environment condition requiring for a CB germination test by International Seed Testing Association (ISTA) was applied in the Experiment I, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$(ISTA conditions). Experiment II was conducted under a room temperature condition of 5 to $25^{\circ}C$(natural conditions). In each experiment, data such as seed germinating vigor, early germination characteristics, germination speed and germination peak time were measured. Significant differences were observed in seed germinating vigor, early germination characteristics, germination speed and germination peak time. Seed germinating vigor was variable with different environments and varieties. It was 61.50 to 98.25% under ISTA conditions and 55.00 to 98.50% under natural conditions. There were considerable variations in early germination characteristics among CB varieties according to different conditions. Early germination characteristics indicated that all varieties were 1 to 4 days faster in germination under ISTA conditions, when compared with natural conditions. The germination speed, measured as days to seed germination of 70% and 90%, was much faster with Penn A-1, Penn A-4 and Penncross under ISTA conditions. But it was even faster with L-93 and Penncross when grown under natural conditions. Differences were also observed in germination peak time with varieties and growing conditions. It was 0.57 to 2.86 days under ISTA conditions and 0.74 to 1.74 days under natural conditions. Regardless of the environment conditions, the shortest variety was L-93 and the longest one T-1. Considering germinating vigor, early germination characteristics, germination speed and germination peak time, Penn A-1, Penn A-4 and Penncross were regarded as excellent varieties under ISTA conditions in terms of early establishment characteristics, while L-93, Penn A-1 and Penncross under natural conditions. These results suggest that an intensive germination test be needed prior to planting, for the early germinating vigor, germination speed and germination peak time. Also, a proper variety selection and comprehensive site analysis for the growing environmental conditions should be done before golf course construction.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.3
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• pp.72-82
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• 2009

Research was initiated to investigate early establislment characteristics and germination pattern of perennial ryegrass (PR, Lolium perenne L.). Eight varieties of PR were evaluated in the study. An alternative environmental condition for a PR germination test required by International Seed Testing Association (ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Daily and cumulative germination pattern were measured and analyzed daily for 30 days. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 75% germination, and final germination rate. Germination rates were variable among the varieties, being 66.00 to 93.75% at the end of study. There were considerable variations in early germination characteristics and germination pattern among PR varieties. The first germination in all entries tested was initiated from 3 to 5 DAS (days after seeding) under ISTA conditions, being 2 days in differences among the varieties. It was 4 to 7 DAS in days to the 50% germination, being 1 to 3 days later from the first germination and three days were variable among them. Days to the 75% germination were 5.93 to 10.50 DAS under ISTA conditions, being 4.57 days in variety differences. Pennant II and Brightstar II were the fastest varieties. The slowest ones were Revenge GLX and Brightstar SLT. Considering days to the first germination, days to 75% germination, and germination pattern etc, Pennant II and Brightstar II were regarded as excellent varieties under ISTA conditions. From tills study, information on differences in germination characteristics and pattern would be usefully applied for golf course design and construction, when established with PR.

• Weed & Turfgrass Science
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• v.2 no.3
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• pp.274-282
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• 2013

Research was initiated to investigate early germination and establishment characteristics of perennial ryegrass (PR, Lolium perenne L.). An alternative condition was applied in Experiment I with 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA condition). Experiment II was conducted under $5^{\circ}C$ to $25^{\circ}C$ (natural condition). Significant differences were observed in germination percentage, germination speed and germination peak time. Seed germinating vigor was 66.00 to 93.75% under ISTA and 66.25 to 93.50% under natural conditions. Germination speed, measured as days to germination of 50% through 90%, was much faster with 'Pennant II', 'Brightstar II', 'Sonata' and 'Accent II' under ISTA conditions. But it was faster only with 'Pennant II' and 'Brightstar II' under natural conditions. Germination peak time ranged 1.48 to 5.65 days under ISTA, while 2.17 to 10.63 days under natural conditions. Regardless of growing conditions, the shortest cultivars in peak time were 'Sonata', 'Pennant II', and 'Brightstar II' and the longest one 'Revenge GLX'. Considering germinating vigor, early germination speed and germination peak time, 'Pennant II', 'Brightstar II', 'Sonata' and 'Accent II' were regarded as excellent cultivars under ISTA conditions, while 'Pennant II' and 'Brightstar II' under natural conditions.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.5
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• pp.1-17
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• 2014

Research was initiated to investigate early establishment characteristics and germination pattern of Kentucky bluegrass (KB, Poa pratensis L.) under natural room temperature and to analyze differences between alternative and natural conditions for a practical application. Fifteen cultivars of KB were evaluated in the study. Daily and cumulative germination pattern were measured and analyzed on a daily basis. Differences were observed in germination percentage, germination pattern, days to the first germination and days to 75% germination. Germination percentage was variable with cultivars at the end of study. It was 75.75 to 90.25% under natural condition. There were considerable variations in early germination characteristics and germination pattern among KB cultivars according to germination conditions. The first germination in all entries tested was initiated between 6 and 9 DAS (days after seeding) under ISTA (International Seed Testing Association) condition. But it was observed between 9 and 12 DAS under natural condition, being 3 to 5 days later as compared with ISTA condition. On the first date of a germinating process, the germination rate was 0.25% to 25.00% under ISTA condition, while 0.50% to 13.25% under natural condition. Days to the 75% germination were 15.08 to 28.80 DAS under ISTA condition and 17.78 to 28.75 DAS under natural condition. Midnight II, Excursion and Midnight were the fastest cultivars under ISTA condition, while Odyssey the fastest one under natural condition. Regardless of germination condition, the slowest cultivar was Voyager II, being over 4 weeks to 75% germination percentage. For the most cultivars, days to 75% germination under natural room temperature condition were 1 to 8 days slower than under ISTA condition. From this study information on differences in germination characteristics and pattern among cultivars would be usefully applied for KB establishment such as golf course construction. Prior to on-site seeding, a comprehensive germination test is practically recommended due to a possible decline in germination capability during domestic distribution and storage after import.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.3
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• pp.47-56
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• 2018

Research was initiated to investigate germination characteristics and germination pattern of 14 herbaceous plant entries used on the road slope during 30 days. An alternative germination condition for 14 herbaceous plant entries required by International Seed Testing Association(ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$. Significant differences were observed in the first germination rate(0.3%~40.7%)and in the final germination rate(7.7%~93.3%). Days to the first germination(2~8days), days to the 30% germination(2~6days) and days to the peak germination(6~18days) were different among 14 herbaceous plant entries in the study. From this result, we could find out higher final germination rate of 14 herbaceous plant entries in the following order; First, forage crops and cool-season turfgrasses; Second, herbeceous flowers; Third, wild plants. We could also divide germination rate among 14 herbaceous plant entries as 6 groups(I;very high, II;high, III;medium high, IV;medium low, V;low, VI;very low) based on the final germination rate and divide germination speed as 5 groups(A;very fast, B;fast, C;normal, D;slow, E;very slow)based on days to the peak germination. Considering germination characteristics and pattern of 14 herbaceous plant entries Medicago sativa, Lolium perenne, Festuca arundinacea and Cosmos sulphureus were regard as dominating species while Lespedeza cuneata, Silene armeria, Lotus corniculatus var.japonicus, Coreopsis tinctoria and Centaurea cyanus as competitive species following dominating species. However, Chrysanthemum burbankii, Pennisetum alopecuroides, Chrysanthemum boreale., Artemisia princeps var. orientalis and Arundinella hirta were not almost expected to emerge.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.23-34
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• 2009

Research was initiated to investigate early establishment characteristics and germination pattern of tall fescue(TF, Festuca arundinacea Shreb.). Four varieties of TF were evaluated in the study. Experiment was conducted under a room temperature condition of $5^{\circ}C$ to $25^{\circ}C$(natural conditions). Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination patterns, days to the first germination, days to 50% germination, days to 75% germination, and germination percentage with different environments and varieties. Germination percentage was variable with varieties at the end of study. It was 85.25 to 97.00% under natural conditions. There were considerable variations in early germination characteristics and patterns among the entries according to different conditions. The first germination was initiated between 7 and 9 DAS(days after seeding) under natural conditions, being 2 to 3 days later as compared with ISTA conditions. It was 11 to 12 DAS in days to the 50% germination, which was 3 to 4 days after the first germination. Days to the 75% germination were 12.38 to 14.29 DAS, being 1.55 to 4.77 days slower as compared with ISTA conditions. Olympic Gold was regarded as the fastest variety and Tar Heel II was slowest. Data from this study demonstrated that information on differences in germination characteristics and patterns among varieties would be usefully applied for TF establishment such as golf course construction.

• Asian Journal of Turfgrass Science
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• v.24 no.2
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• pp.79-87
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• 2010

Research was initiated to investigate the early establishment characteristics and germination patterns of perennial ryegrass (PR, Lolium perenne L.). Eight cultivars of PR were evaluated in the study. Experiment was conducted under a room temperature condition of $6^{\circ}C$ to $23^{\circ}C$ (natural conditions). Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 75% germination, and germination percentage with different environments and cultivars. Germination percentage was variable with cultivars at the end of study. It was 66.25 to 93.50% under natural conditions. There were considerable variations in the early germination characteristics and germination pattern among the entries according to different conditions. The first germination was initiated between 3 and 5 DAS (days after seeding) under ISTA (International Seed Testing Association) conditions. But it started between 6 and 8 DAS under natural conditions, being 2 to 4 days later as compared with that under ISTA conditions. It was 8 to 12 DAS that reached to days to the 50% germination, which was 2 to 5 days after the first germination. Days to the 75% germination were 10.08 to 13.70 DAS under natural conditions, being 2.07 to 5.70 days slower as compared with ISTA conditions. 'Pennant II' was the fastest cultivar. But the slowest was 'Catalina II' that was 3.62 days later than 'Pennant II' under natural conditions. Considering days to the first germination, days to 75% germination, and germination pattern etc, 'Pennant II' and 'Brightstar II' were regarded as excellent cultivars under both conditions. From this study, information on differences in germination characteristics and patterns would be usefully applied for golf course design and construction, when established with PR.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.4
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• pp.30-41
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• 2010

Research was initiated to investigate germination characteristics and germination pattern of creeping bentgrass (CB, Agrostis palustris Huds.). Seven varieties of CB were evaluated in the study. An alternative condition for a CB germination test required by International Seed Testing Association (ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 85% germination, and germination percentage. Germination rates were different among the treatments at the end of study. There were significantly considerable variations in early germination characteristics and germination pattern among CB varieties. The first germination in all entries was initiated between 4 and 5 DAS (days after seeding) under ISTA conditions. Generally, the first germination percentage was 5 to 25%. However, such Penn-series varieties as Penn A-1, Penn A-4 and Penncross were much more vigorous over others, resulting in 50 to 75% in the first germination percentage. It took 4 to 10 DAS in reaching to days to the 50% germination, being 5 days in difference among the varieties. Days to the 85% germination were 5.90 to 11.75 DAS under ISTA conditions, being 5.85 days in variety difference. It means 1 to 2 days after days to 50% germination. Penn A-1 was the fastest variety, while T-1 the slowest one. Considering days to the first germination, days to 85% germination, and germination pattern etc, Penn A-1, Penn A-4, Penncross and L-93 were regarded as excellent varieties under ISTA conditions. From this study, in-depth information on germination characteristics and pattern would be usefully applied for golf course design and construction, when established with CB.