• 제목/요약/키워드: IS6110

검색결과 531건 처리시간 0.023초

PCR 기법을 이용한 도축 소의 결핵병 신속진단 (Rapid diagnosis of bovine tuberculosis in slaughter cattle using PCR)

  • 고바라다;김현중;박덕웅;박성도;김재익;박종태;김용환
    • 한국동물위생학회지
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    • 제30권3호
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    • pp.393-406
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    • 2007
  • Bovine tuberculosis is an important zoonosis worldwide. Mycobacterium bovis, the causative agent of this disease in cattle, is also a pathogen for humans and several economically important animals. The cases of tuberculosis are reported in two cow found at slaughter house located in Gwangju city. Histopathologically, in the lymph nodes, granulomas consisted of large areas of necrosis surrounded by variable thick bands of cellular infiltrate containing macrophages, Langhans-type multinucleated giant cells and lymphocytes. Lesions in the lung followed the same developmental pattern as did lesions in the lymph nodes with some exceptions. With the acid-fast staining, numerous mycobacteria were revealed in the lung and lymph nodes. M bovis was confirmed as a causative agent in these cattle using bacterial isolation and PCR and restriction fragment length polymorphism method based on a unique 12.7 kb fragment insertion sequence from the Mycobacterium tuberculosis genome and the pncA polymorphism, The insertion element IS6110 and IS1081 were present M bovis isolated from lungs and lymph nodes of cattle using PCR assay. These cases are interesting and important in public health aspect that M bovis-infected cattle were found during a routine post-mortem inspection at slaughter house.

PCR이란 무엇인가

  • 박영길
    • 보건세계
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    • 제40권12호통권448호
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    • pp.26-28
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    • 1993
  • 세포나 미생물의 특정 DNA 부위를 증폭하고자 할때 이용되는 PCR(polymerase chain reaction, 핵산중합효소 연쇄반응)은 1983년 미국 Cetus사의 Mullos에 의해 고안되었고, 그후 PCR에 의한 마이코 박테리아의 동정 및 검출이 특정 염기서열의 연구가 이루어지면서 활발히 시도되었으며, 아이젠하크 등은 IS6110을 이용하여 본격적으로 PCR이 결핵균 검출에 유용한 도구로 사용될 수 있다는 가능성을 제시하였다. PCR에 의한 DNA증폭은 단시간 내에 이루어진다는 장점 때문에 유전병 및 미생물의 진단에 이용될 뿐 아니라 법의학 분야 및 장기이식에 따른 조직접합 시험에도 이용될 것으로 예상된다. 이에 따라 국내에서도 결핵연구원에서 최초로 일반 임상검사에 적용함으로써 현재 많은 호평을 받고 있는 PCR에 대하여 알아본다

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열회수 냉각 제습기의 이론적 해석 (Theoretical Analysis of a Recuperative Refrigeration Dehumidifier)

  • 김동선
    • 설비공학논문집
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    • 제26권1호
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    • pp.48-54
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    • 2014
  • A refrigeration dehumidifier with a recuperative heat exchanger is theoretically analyzed. The recuperative heat exchanger is located between the two air streams from and to the dehumidifying coil, and reduces the sensible heat load in dehumidification process. A simple model is developed to predict performance of the dehumidifier. The model predicts that the recuperative heat exchanger is effective especially in the low humidity condition, where the sensible heat load is relatively large. It is predicted that, by adopting a recuperative heat exchanger, a maximum 30~110% increase in COP is possible for indoor air at $27^{\circ}C$, and 40~60% relative humidity.

사이클론 방식 유분리기의 출구 튜브 설계 (Design of the Outlet-Port Tube of a Cyclone-Type Oil Separator for a Compressor)

  • 장성일;안준
    • 설비공학논문집
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    • 제27권8호
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    • pp.402-408
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    • 2015
  • A series of numerical simulations have been carried out to study the performances of cyclone-type oil separators, which are designed for refrigeration-system compressors. The corresponding working fluid is R22, which is a typical refrigerant, whereby a mineral-oil droplet is supplied (Ed-highlight-My interpretation). The outlet-tube length in relation to the total chamber volume is considered a design parameter. Depending on the tube length, the separation efficiency varies from 98.7% to 99.3%, while the predicted pressure drop is between 5.1 kPa and 6.4 kPa. Considering both the pressure drop and separation efficiency, the length of the outlet-port tube of the separator is 152 mm.

태양열 콤비시스템의 축열조에 적용되는 분배기의 효과 (The Effect of a Manifold in a Storage Tank Applied to a Solar Combisystem)

  • 손효석;홍희기
    • 설비공학논문집
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    • 제26권7호
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    • pp.322-328
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    • 2014
  • Return piping is used in a solar combi-system for heating and hot water supply. When the temperature of the lower side of a storage tank is low due to hot water usage, the returned hot water after heating is mixed with the lower side cold water of the tank, and the useful energy is reduced. We studied the degree of thermal stratification in the tank, using either a diffuser or a manifold to prevent mixing. Using the diffuser, mixing starts from the bottom of the storage tank. On the other hand, the manifold has the marked effect of preventing mixing. As a result of experiments with changing the diameter and number of holes in the manifold, the optimum condition is 8.5 mm diameter and 96 holes, under the condition of 0.3 lpm.

판형 흡착열교환기를 사용한 폴리머-물 흡착식 냉동기의 성능예측 (Simulation of a Polymer-Water Adsorption Refrigerator using Plate-Type Adsorption Heat Exchangers)

  • 김동선;이대영
    • 설비공학논문집
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    • 제27권2호
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    • pp.94-102
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    • 2015
  • A hydroscopic polymer is used as the adsorbent in an adsorption refrigeration system. A pair of plate-type heat exchangers, thinly coated with the polymer, is simulated using a two-dimensional transient model to predict performance of the system. It is predicted that the system would yield 0.57 kW SCP and 0.47 COP at $80^{\circ}C$ heating and $30^{\circ}C$ cooling temperatures. In comparison with a conventional silica gel-water system, the COP is comparable but SCP is about three times larger. The slow mass diffusion rate of the polymer should be improved for better performance.

열역학적 사이클의 제2법칙 효율의 정의에 대한 기본 연구 (Basic Study on the Definition of the Second Law Efficiencies of Thermodynamic Cycles)

  • 박경근
    • 설비공학논문집
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    • 제24권11호
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    • pp.792-798
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    • 2012
  • A general concept on the definition of the second law efficiencies of thermodynamic cycles is introduced. The efficiency is defined to be proportional to the entropy generation divided by the maximum possible entropy generation. This way of definition of the cycle efficiency is clear and concise and, moreover, follows faithfully the concept of the second law of thermodynamics. This definition is applied to heat engine, refrigerator and heat pump. The second law efficiencies of heat engine and refrigeration cycles are derived, which are the same as the existing ones, respectively. The second law efficiency of heat pump, however, finds to be different from the existing one. Discussion is given about the difference and its cause.

열전모듈을 이용한 자동차용 1 kW급 보조 냉난방 시스템의 성능에 관한 실험적 연구 (An Experimental Study on the Supplemental Cooling and Heating Performance Using 1 kW Thermoelectric Module for Vehicle)

  • 이대웅
    • 설비공학논문집
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    • 제26권5호
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    • pp.224-230
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    • 2014
  • The purpose of this paper is to investigate the performance of supplemental cooling and heating system equipped with the 1 kW thermoelectric module. The system consist of 96 thermoelectric modules, heat sink with louver fin and water cooling jacket which is attached on the hot side of the thermoelectric module. The cooling and heating performance test of the thermoelectric system is conducted with various conditions, such as intake voltage, air inlet temperature, air flow volume, water inlet temperature and water flow rate at calorimeter chamber in consideration of environmental conditions in realistic vehicle drive. The experimental results of a thermoelectric system shows that the cooling capacity and COP is 1.03 kW, and 1.0, and heating capacity and COP is 1.53 kW, and 1.5 respectively.

대규모 실내경기장의 성능위주 방재계획에 관한 연구 (A Study on Perfomance Based Evacuation Plan for a Large Indoor-Arena)

  • 최용석;김형근;이규일
    • 설비공학논문집
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    • 제23권10호
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    • pp.687-698
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    • 2011
  • In this study, it was analyzed by a numerical analysis that plan/design considerations for ensuring the spectator safety of large arena audiences in a fire emergency evacuation plan. The latest issue, the 'performance-based design', fire and evacuation plan is important. But nowadays 'Specification-based design' is in common. In evacuation simulation, congestion of exit and aisle is ignored because only evacuation time of large-space is mainly analyzed. In smoke flow,'smoke filling effect' tends to be overrated. From now on, when design a field house, it is needed not 'smoke filling effect' and 'large-space evacuation' analysis, but analyzing 'whole building evacuation time' for ensuring fire evacuation safety of spectator.

지열발전용 배관재(SUS316)의 부식특성 평가 (Evaluation of Corrosion Characteristics of Pipeline Material(SUS316) for the Geothermal Power Plant)

  • 한지원;박영수
    • 설비공학논문집
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    • 제24권2호
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    • pp.142-146
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    • 2012
  • In the geothermal power, the geothermal fluid such as water or steam is moved from the ground to turbine a pipeline. Because the geothermal fluid with high temperature contains Cl-and SO4-, It cause to diminish the system lifetime due to the increase in the corrosion of pipeline. In the present work, the characteristics of corrosion and its fatigue of the sus316 which is used in the pipeline are evaluated experimentally. From this study, the following results can be obtained; for the case of the corrosion environment, it is found that the corrosion rate is faster than that of the steam environment by 10 to 30 times, and the corrosion fatigue limit is underestimated compared to that of steam state.