• Title/Summary/Keyword: IRS1

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Comparative Chloroplast Analysis and Phylogenetic Relationships Among Corylopsis Siebold & Zucc. (Hamamelidaceae)

  • Tae-Hee Kim;Sang-Chul Kim;Young-Ho Ha;Hiroaki Setoguchi;Hyuk-Jin Kim
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.55-55
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    • 2022
  • Corylopsis Siebold & Zucc. (Hamamelidaceae) is widely used for horticultural plant and comprise ca. 25 species in East Asia (1 species in Korea; 4 species in Japan; 20 species in China). Previous revisions have gone from 7 to more than 30 species, causing confusion in the nursery industry and public gardens. Due to morphological similarity within Corylopsis, molecular research is needed to distinguish it. In this study, the chloroplast genome of C. gotoana and C. pauciflora distributed in Japan was completed by using NGS (Next-Generation Sequencing) technique. The genome size of C. gotoana and C. pauciflora were 159,434 bp (large single-copy (LSC): 88,164 bp; small single-copy (SSC): 18,702 bp; inverted repeat regions (IRs): 26,284 bp) and 159,363 bp (LSC: 88,097 bp; SSC: 18,700 bp; IRs: 26,283 bp), respectively. In addition, we investigated the repeats, SNPs, and indels, and that could be used as DNA markers. Phylogenetic analysis demonstrated that C. pauciflora was sister to C. gotoana and C. spicata. The genus Corylopsis is a monophyletic group and Loropetalum is closely related to Corylopsis. The results of our study will provide the basic data necessary for the analysis of the species identification markers and genetic diversity within the genus Corylopsis in the future.

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A Study on Management Efficiency of Korean Ship Component Part Manufacturers

  • Kim, Myung-Jae
    • Journal of Navigation and Port Research
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    • v.34 no.7
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    • pp.577-585
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    • 2010
  • This paper analyzes the efficiency of Korean Ship Component Part Manufacturer firms using DEA model. We evaluated the CCR, BCC efficiency and RTS of 30 Korea Ship Component Part Manufacturer firms. We also suggested the Korean Ship Component Part Manufacturer firms that can be benchmarked based on analyzed information. The result showed five enterprises whose values of CCR efficiency are 1, and eleven enterprises whose values of BCC efficiency are 1. RTS indicated IRS of 1 firms, DRS of 24 firms and CRS of 5 firms.

Effects of Endurance Exercise and Ginsenoside Rb1 on AMP-Activated Protein Kinase, Phosphatidylinositol 3-Kinase Expression and Glucose Uptake in the Skeletal Muscle of Rats (지구성 운동과 Ginsenoside Rb1가 쥐 골격근의 AMP-Activated Protein Kinase(APMK), Phosphatidylinositol 3-Kinase(PI3K) 발현 및 Glucose Uptake에 미치는 영향)

  • Jung, Hyun-Lyung;Shin, Young Ho;Kang, Ho-Youl
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.8
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    • pp.1197-1203
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    • 2013
  • This study investigated the effects of endurance exercise and ginsenoside $Rb_1$ on AMP-activated protein kinase (AMPK), phosphatidylinositol 3-kinase (PI3K) protein expression and glucose uptake in the skeletal muscle of rats. A total of 32 rats were randomly divided into four groups: CON (Control group, n=8), Ex (Exercise group; 25 m/min for 1 h, 6 days/week, 2 weeks, n=8), $Rb_1$ (Ginsenoside $Rb_1$ group; n=8), and $Rb_1/Ex$ ($Rb_1$+Exercise group, n=8). The $Rb_1$ and $Rb_1/Ex$ groups were incubated in ginsenoside $Rb_1$ (KRBP buffer, $100{\mu}g/mL$) for 60 min after a 2-week experimental treatment. After 2 weeks, the expression of phosphorylated $AMPK{\alpha}$ $Thr^{172}$, total $AMPK{\alpha}$, the p85 subunit of PI3K, pIRS-1 $Tyr^{612}$, and pAkt $Ser^{473}$ were determined in the soleus muscle. Muscle glucose uptake was measured using 2-deoxy-D-[$^3H$] glucose in epitroclearis muscle. Muscle glucose uptake was significantly higher in the three experimental groups (Ex, $Rb_1$, $Rb_1/Ex$) compared to the CON group (P<0.05). The expression of $tAMPK{\alpha}$ and $pAMPK{\alpha}$ $Thr^{172}$ was significantly higher in the Ex, $Rb_1$, and $Rb_1/Ex$ groups compared to the CON group (P<0.05). The expression of pAkt $Ser^{473}$ was significantly higher in the $Rb_1$ group compared to the CON and EX groups. However, the expression of pIRS-1 $Tyr^{612}$ and the p85 subunit of PI3K were not significantly different between the four groups. Overall, these results suggest that ginsenoside $Rb_1$ significantly stimulates glucose uptake in the skeletal muscle of rats through increasing phosphorylation in the AMPK pathway, similar to the effects of exercise.

Infrared and Radio observations of a small group of protostellar objects in the molecular core, L1251-C

  • Kim, Jungha;Lee, Jeong-Eun;Choi, Minho;Bourke, Tyler L.;Evans II, Neal J.;Di Francesco, James;Cieza, Lucas A.;Dunham, Michael M.
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.1
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    • pp.67.4-68
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    • 2015
  • We present a multi-wavelength observational study of a low-mass star-forming region, L1251-C, with observational results at wavelengths from the near-infrared to the millimeter. Spitzer Space Telescope observations confirmed that IRAS 22343+7501 is a small group of protostellar objects. The extended emission to east-west direction with its intensity peak at the center of L1251A has been detected at 350 and 850 mm with the CSO and JCMT telescopes, tracing dense envelope materials around L1251A. The single-dish data from the KVN and TRAO telescopes show inconsistencies between the intensity peaks of several molecular line emission and that of the continuum emission, suggesting complex distributions of molecular abundances around L1251A. The SMA interferometer data, however, show intensity peaks of CO 2-1 and $^{13}CO$ 2-1 located at the position of IRS 1, which is both the brightest source in IRAC image and the weakest source in the 1.3 mm dust continuum map. IRS 1 is the strongest candidate for being the driving source of a newly detected the compact CO 2-1 outflow. Over the whole region ($14^{\prime}{\times}14^{\prime}$) of L125l-C, 3 Class I and 16 Class II sources have been detected, including three YSOs in L1251A. A comparison with the average projected distance among 19 YSOs in L1251-C and that among 3 YSOs in L1251A suggests L1251-C is an example of low-mass cluster formation, where protostellar objects are forming in a small group.

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Changes in expression of insulin signaling pathway genes by dietary fat source in growing-finishing pigs

  • Kim, Seung-Chang;Jang, Hong-Chul;Lee, Sung-Dae;Jung, Hyun-Jung;Park, Jun-Cheol;Lee, Seung-Hwan;Kim, Tae-Hun;Choi, Bong-Hwan
    • Journal of Animal Science and Technology
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    • v.56 no.4
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    • pp.12.1-12.7
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    • 2014
  • This study investigated changes in gene expression by dietary fat source, i.e., beef tallow, soybean oil, olive oil, and coconut oil (each 3% in feed), in both male and female growing-finishing pigs. Real-time PCR was conducted on seven genes (insulin receptor; INSR, insulin receptor substrate; IRS, phosphatidylinositol (3,4,5)-triphosphate; PIP3, 3-phosphoinositide-dependent protein kinase-1; PDK1, protein kinase B; Akt, forkhead box protein O1; FOXO1 and cGMP-inhibited 3', 5'-cyclic phosphodiesterase; PDE3) located upstream of the insulin signaling pathway in the longissimus dorsi muscle (LM) of pigs. The INSR, IRS, PIP3, and PDE3 genes showed significantly differential expression in barrow pigs. Expression of the PIP3 and FOXO1 genes was significantly different among the four dietary groups in gilt pigs. In particular, the PIP3 gene showed the opposite expression pattern between barrow and gilt pigs. These results show that dietary fat source affected patterns of gene expression according to animal gender. Further, the results indicate that the type of dietary fat affects insulin signaling-related gene expression in the LM of pigs. These results can be applied to livestock production by promoting the use of discriminatory feed supplies.

The Effect of Integration Between Respiratory Muscle Training and Abdominal Drawing-in Maneuver on Decreased Pulmonary Function in Young Subjects

  • Kim, Chang-Yong;Choi, Jong-Duk;Byun, Dong-Wook;Kim, Jin-Seok;Lee, Ji-Yeol
    • Physical Therapy Korea
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    • v.18 no.4
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    • pp.26-33
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    • 2011
  • The aim of this study was to investigate the effects of respiratory muscle training (RMT) with abdominal drawing-in maneuver (ADIM) on pulmonary function. Twenty-two subjects with restrictive breathing participated in this study. All the subjects were randomly assigned to three groups (7 subjects in RMT group, 7 subjects in RMT with ADIM group, 8 subjects in control group). The first group performed the RMT by using incentive respiratory spirometer (IRS). The second group performed the RMT by using IRS and the ADIM by using a Stabilizer. The exercises were conducted over four days. The pulmonary function was evaluated using the spirometer to measure the force exploratory volume in 1 second ($FEV_1$) and forced vital capacity (FVC). Measurements were conducted on the first day and the last day. A paired-t test was used for pre-post changes and the change rates in FVC and $FEV_1$ among each group were investigated by a one-way ANOVA. The findings of the the study were as follows: 1) There were significant differences of FVC and $FEV_1$ between pre and post in the two training groups (p<.05) 2) There was no significant difference of the change ratio the FVC and $FEV_1$ between the RMT group and RMT with ADIM group. Therefore, it is concluded that respiratory muscle and ADIM training, combined with two methods of treatment would suggest positive evidence for improving pulmonary function.

Differentially Expressed Genes in Metastatic Advanced Egyptian Bladder Cancer

  • Zekri, Abdel-Rahman N;Hassan, Zeinab Korany;Bahnassy, Abeer A;Khaled, Hussein M;El-Rouby, Mahmoud N;Haggag, Rasha M;Abu-Taleb, Fouad M
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.8
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    • pp.3543-3549
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    • 2015
  • Background: Bladder cancer is one of the most common cancers worldwide. Gene expression profiling using microarray technologies improves the understanding of cancer biology. The aim of this study was to determine the gene expression profile in Egyptian bladder cancer patients. Materials and Methods: Samples from 29 human bladder cancers and adjacent non-neoplastic tissues were analyzed by cDNA microarray, with hierarchical clustering and multidimensional analysis. Results: Five hundred and sixteen genes were differentially expressed of which SOS1, HDAC2, PLXNC1, GTSE1, ULK2, IRS2, ABCA12, TOP3A, HES1, and SRP68 genes were involved in 33 different pathways. The most frequently detected genes were: SOS1 in 20 different pathways; HDAC2 in 5 different pathways; IRS2 in 3 different pathways. There were 388 down-regulated genes. PLCB2 was involved in 11 different pathways, MDM2 in 9 pathways, FZD4 in 5 pathways, p15 and FGF12 in 4 pathways, POLE2 in 3 pathways, and MCM4 and POLR2E in 2 pathways. Thirty genes showed significant differences between transitional cell cancer (TCC) and squamous cell cancer (SCC) samples. Unsupervised cluster analysis of DNA microarray data revealed a clear distinction between low and high grade tumors. In addition 26 genes showed significant differences between low and high tumor stages, including fragile histidine triad, Ras and sialyltransferase 8 (alpha) and 16 showed significant differences between low and high tumor grades, like methionine adenosyl transferase II, beta. Conclusions: The present study identified some genes, that can be used as molecular biomarkers or target genes in Egyptian bladder cancer patients.

Outflow properties of 24 DIGITembedded soruces

  • Kang, Seonmi;Lee, Jeong-Eun;Choi, Minho;Evans, Neal J.;Dunham, Michael M.
    • The Bulletin of The Korean Astronomical Society
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    • v.38 no.2
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    • pp.65.2-65.2
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    • 2013
  • We present a study of outflows on 24 embedded young stellar objects (YSOs), which are selected from the sources of the Dust, Ice, and Gas in Time (DIGIT) Herschel key program. Molecular outflow activity, which is believed to have strong dependence on accretion process, is the most powerful in the early embedded phase of star formation and declines as the central protostars evolve to the main sequence stage. In order to study the relation between the CO outflow observed in low J transitions and the properties of protostars, we mapped the CO outows of the selected targets in J = 1-0 and J = 2-1 lines with the 14-m TRAO telescope and the 6-m SRAO telescope, respectively. We estimate CO outflow momentum fluxes (Fco) and compare with bolometric luminosity, Lbol, bolometric temperature, Tbol, and the FIR molecular line luminosities of CO, $H_2O$, OH and [O I], which were detected by the Herschel-PACS observations. We found that $Fco_{1-0}$ is greater than $Fco_{2-1}$, and the mean ratio is about 2. L1455-IRS3 and IRAM04191 have high Fco in spite of low $L_{bol}$. The well known correlation between Fco and $L_{bol}$. is not very evident from all our samples. However, Fco and $L_{bol}$. show a rather strong correlation if L1455-IRS3 and IRAM04191 are excluded. Fco shows little correlation with FIR line luminosities of individual species, while the total FIR line luminosity summed over CO, $H_2O$, OH, and [OI] lines seems to have some correlation. In addition, we report 22 GHz $H_2O$, and 44 GHz CH3OH maser line detections in four sources out of the 24 YSOs.

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Radio Observation of L1521F using HCN (J=1-0) Line (L1521F의 HCN(J=1-0) 분자선 전파 관측)

  • Sohn, Jung-Joo;Lee, Chang-Won
    • Journal of the Korean earth science society
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    • v.31 no.4
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    • pp.370-377
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    • 2010
  • In this study, we investigated the kinematical properties of the L1521F-IRS in Taurus region using HCN (J=1-0) molecular line. The high resolution mapping has carried out by $5{\times}5$ point observations covering $3.7'{\times}3.7'$ area using a 12-m telescope of Arizona Radio Observatory in Tucsan, USA. L1521F which harbors the faint infrared L1521F-IRS, displayed a strong central concentration of integrated intensity in HCN without serious molecular depletion. It showed a symmetric kinematical structure with the opposite infall motion in either side of the central cores. It is a direct evidence of bipolar outflows in the core of L1521F.

암흑성운 Baenars 5의 H$_2$CO 2mm 와 6cm의 관측

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    • Bulletin of the Korean Space Science Society
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    • 1993.04a
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    • pp.12-12
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    • 1993
  • 암혹성운 B5 영역의 중심 부분을 분자흡수선 BSCO의 6cm와 방출선인 2mmE 관측하였다. 관측은 독일의 Bonn에 있는 전파망원경과 미국의 Arizona주의 Kit Peak에 있는 전파망원정을 사용하였다. 본 관측 결과에서 얻어진 antenna temperature (Ta)의 분포를 mappin 하였다. 이 두 종류의 판측을 상호 비교한 결과 최대 황도의 위치가 서로일치하지 않음을 확인하였다. 6cm의 관측결과는 star count 방법에 의하여 결정된 extionction map에 나타나는 중심지역과 거의 일치하나, 2mm 관측은 B5 내에 있는 적외선광인 IRS 1의 위치와 일치하였다. 본 연구에서는 다른 분자선 관측 결과와 비교하므로써 H2CO의 관측 결과를 분석하였다.

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