• Journal of Korean Society for Quality Management
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• v.35 no.1
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• pp.97-112
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• 2007

Six Sigma methodology has been evolved since it was first introduced in late 1980's and the number of company which adopts Six Sigma is getting increased. Therefore, it is timely that ire review research papers on Six Sigma extensively. We review 85 papers published in Korea by April 2006 and classify them based on research contents and methodology. We suggest future research issues and fields which can adopt Six Sigma methodology.

• Fire Science and Engineering
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• v.8 no.1
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• pp.26-33
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• 1994

The review of field model for fire simulation is presented. The approach to predict ire growth by solving the conservation equations over time and 2- or 3-dimentional space with appropriate boundary conditions is called field model. The finite difference method is adopted to solve the governing equations. The results is give qualititively and quantitatively good results but complicate and difficult to handle. But it is useful tool to understand detail phenomenons and to make decision for fire safety.

• Journal of Life Science
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• v.22 no.2
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• pp.281-284
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• 2012

This study demonstrated that upregulation of gene expression of endoplasmic reticulum (ER) stress chaperones (Bip, ERp29, calnexin, and PDI), ER stress sensors (PERK, ATF6, and Ire1), and cAMP phosphodiesterase 7A1 (cAMP PDE7A1) was induced by ER stresses in FRTL5 cells. While removing A23187 from the culture medium restored upregulation of cAMP PDE7A1 gene expression, removal of thapsigargin did not recover its expression. In addition, cAMP PDE7A1 gene expression was strongly inhibited by treatment with A23187 combined with thyroid stimulating hormone (TSH). The results are the first to show that ER stress induces cAMP PDE7A1 gene expression.

• Journal of Information Management
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• v.26 no.1
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• pp.52-79
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• 1995

The objective of this study is to apply Information Resource Management(IRM) methodology to corporate library and information units. The focus of the study fixes how to start, develop and establish IRM in corporations. The methodology of IRM is based on the way of integrating information contents and systems by information technology. The body of this study consists of three parts. Part 1 surveys the review of IRM progress, its definitions, its components, IRM policy, and IRM human resource. Part 2 suggests a modeling for IRM methodology, which includes the inventory for pinpointing each information resource entity(IRE), the analysis of information needs, the measuring cost of IRE, and the evaluation of information value. Part 3 investigates internal and external information resources of certain corporations and analyses the users' information needs.

• Journal of Broadcast Engineering
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• v.15 no.1
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• pp.76-88
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• 2010

Recently, LED background image devices are used for the production of broadcasting programs. But the effective advantages of LED background image devices are not realized for good HD video quality because the correlations between lighting sources and LED background image devices are not analyzed yet. In this paper, we analyze the color change and light intensity that lighting sources and the light intensity ratio of LED background images influence on a subject, making use of equipments used in real broadcasting, and examine the correlations between HD video quality and the light intensity ratio of LED background images. As a result in this experiment, the video is most expressed having an 20[IRE] effect on a subject when the light intensity of LED background image is 6%. When the LED background image is light, the light and darkness of video is distorted and when the LED background image is dark, the colors of image are distorted.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 1997.11a
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• pp.392-403
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• 1997

There is a strong international move towards performance based fire regulations for buildings with New Zealand and Australia at the forefront of research in this fold. The reform of regulations is thought to offer more innovation and flexibility in building design and greater cost effectiveness in construction. An important part of the research in this area is related to the development of agreed approaches to fire safety design, such as the Fire Code Reform Centre's "Fire Engineering Guidelines" or New Zealand's "Fire Engineering Design Guide". Such design process documents have incorporated or referenced much of the latest research in areas such as: tenability criteria fire compartment models egress models risk assessment. Use of such design guidelines or equivalents in major projects in countries such as Hong Kong and Australia have highlighted where fro engineering can offer real benefits to building designers and ultimately building owners and operators. However, there is still much research to be done and use of a systematic, logical design approach clearly identifies where design data or modelling techniques are still urgently required. Such areas are: fire growth rates and peak heat release rates for non-residential occupancies pre-movement times related to egress experimental validation and limits of applicability of CFD and other compartment Ire models probability/reliability data on fire protection systems for risk based analysis. Examples from case studies will be shown where lack of such research and poor judgement can lead to inferior design solutions or where unnecessarily conservative designs can lead to cost excesses. In summary, the link between Ire engineering designers and the research community is very important to highlight areas of fire research that will have the most benefit to the building and construction industry.nstruction industry.

• Journal of Nutrition and Health
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• v.52 no.2
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• pp.176-184
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• 2019

Purpose: Protein overloading in the endoplasmic reticulum (ER) leads to endoplasmic reticulum stress, which exacerbates various disease conditions. Emodin, an anthraquinone compound, is known to have several health benefits. The effect of emodin against palmitic acid (PA) - induced ER stress in HepG2 cells was investigated. Methods: HepG2 cells were treated with varying concentrations of palmitic acid to determine the working concentration that induced ER stress. ER stress associated genes such as ATF4, XBP1s, CHOP and GRP78 were checked using RT- PCR. In addition, the expression levels of unfolded protein response (UPR) associated proteins such as $IRE1{\alpha}$, $eIF2{\alpha}$ and CHOP were checked using immunoblotting to confirm the induction of ER stress. The effect of emodin on ER stress was analyzed by treating HepG2 cells with $750{\mu}M$ palmitic acid and varying concentrations of emodin, then analyzing the expression of UPR associated genes. Results: It was evident from the mRNA and protein expression results that palmitic acid significantly increased the expression of UPR associated genes and thereby induced ER stress. Subsequent treatment with emodin reduced the mRNA expression of ATF4, GRP78, and XBP1s. Furthermore, the protein levels of $p-IRE1{\alpha}$, $p-eIF2{\alpha}$ and CHOP were also reduced by the treatment of emodin. Analysis of sirtuin mRNA expression showed that emodin increased the levels of SIRT4 and SIRT7, indicating a possible role in decreasing the expression of UPR-related genes. Conclusion: Altogether, the results suggest that emodin could exert a protective effect against fatty acid-induced ER stress and could be an agent for the management of various ER stress related diseases.

• Nutrition Research and Practice
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• v.13 no.2
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• pp.95-104
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• 2019

BACKGROUND/OBJECTIVES: Inflammatory Bowel Disease (IBD) has rapidly escalated in Asia (including Korea) due to increasing westernized diet patterns subsequent to industrialization. Factors associated with endoplasmic reticulum (ER) stress are demonstrated to be one of the major causes of IBD. This study was conducted to investigate the effect of Lycium barbarum (L. barbarum) on ER stress. MATERIALS/METHODS: Mouse embryonic fibroblast (MEF) cell line and polarized Caco-2 human intestinal epithelial cells were treated with crude extract of the L. chinense fruit (LF). Paracellular permeability was measured to examine the effect of tight junction (TJ) integrity. The regulatory pathways of ER stress were evaluated in MEF knockout (KO) cell lines by qPCR for interleukin (IL) 6, IL8 and XBP1 spliced form (XBP1s). Immunoglobulin binding protein (BiP), XBP1s and CCAAT/enhancer-binding homologous protein (CHOP) expressions were measured by RT-PCR. Scanning Ion Conductance Microscopy (SICM) at high resolution was applied to observe morphological changes after treatments. RESULTS: Exposure to LF extract strengthened the TJ, both in the presence and absence of inflammation. In polarized Caco-2 pretreated with LF, induction in the expression of proinflammatory marker IL8 was not significant, whereas ER stress marker XBP1s expression was significantly increased. In wild type (wt) MEF cells, IL6, CHOP and XBP1 spliced form were dose-dependently induced when exposed to $12.5-50{\mu}g/mL$ extract. However, absence of XBP1 or $IRE1{\alpha}$ in MEF cells abolished this effect. CONCLUSION: Results of this study show that LF treatment enhances the barrier function and reduces inflammation and ER stress in an $IRE1{\alpha}$-XBP1-dependent manner. These results suggest the preventive effect of LF on healthy intestine, and the possibility of reducing the degree of inflammatory symptoms in IBD patients.

• Animal Bioscience
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• v.35 no.1
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• pp.22-28
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• 2022

Objective: Endoplasmic reticulum (ER) stress engages the unfolded protein response (UPR) that serves as an important mechanism for modulating hepatic fatty acid oxidation and lipogenesis. Chronic fasting in mice induced the UPR activation to regulate lipid metabolism. However, there is no direct evidence of whether negative energy balance (NEB) induces ER stress in the liver of cows. This study aimed to elucidate the relationship between the NEB attributed to feed deprivation and ER stress in bovine hepatocytes. Methods: Blood samples and liver biopsy tissues were collected from 6 non-lactating cows before and after their starvation for 48 h. The blood non-esterified fatty acids (NEFA), β-hydroxybutyric acid (BHBA) and glucose level were analyzed. Real-time quantitative polymerase chain reaction and Western blotting were used to explore the regulation of genes associated with UPR and lipid metabolism. Results: The starvation increased the plasma BHBA and NEFA levels and decreased the glucose level. Additionally, the starvation caused significant increases in the mRNA expression level of spliced X-box binding protein 1 (XBP1s) and the protein level of phosphorylated inositol-requiring kinase 1 alpha (p-IRE1α; an upstream protein of XBP1) in the liver. The mRNA expression levels of peroxisome proliferator-activated receptor alpha and its target fatty acid oxidation- and ketogenesis-related genes were significantly upregulated by the starvation-mediated NEB. Furthermore, we found that the mRNA expression levels of lipogenic genes were not significantly changed after starvation. Conclusion: These findings suggest that in the initial stage of NEB in dairy cows, the liver coordinates an adaptive response by activating the IRE1 arm of the UPR to enhance ketogenesis, thereby avoiding a fatty liver status.

• Journal of Life Science
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• v.28 no.10
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• pp.1186-1192
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• 2018

The purpose of this study was to compare the effects of either aerobic exercise or polyphenols supplementation on mRNA expression of endoplasmic reticulum stress in skeletal muscle of high fat diet-induced obese mice. In the study, mice were divided into five groups: (1) NC (normal diet for 16 weeks as a control, n=10), (2) HC (high fat diet for 16 weeks as a control, n=10), (3) H-Re (high fat diet with resveratrol 25 mg/kg supplementation for 16 weeks, n=10), (4) H-Ch (high fat diet with chrysin 50 mg/kg supplementation for 16 weeks, n=10), and (5) HE (high fat diet with aerobic exercise for 16 weeks, n=10). Aerobic exercise was performed on a treadmill for 40~60 min/day at 10~14 m/min, 0% grade, four days/week for 16 weeks. Endoplasmic reticulum stress related genes were measured by real-time polymerase chain reaction. ATF6, PERK, $IRE1{\alpha}$, and BIP/GRP78 mRNA were significantly decreased in HE compared with those in HC (p<0.05). Also, ATF6, $IRE1{\alpha}$, and BIP/GRP78 mRNA were significantly decreased in H-Re compared with those in HC (p<0.05). ATF6 mRNA was significantly decreased in H-Ch compared with that in HC (p<0.05). These findings suggest that aerobic exercise, resveratrol, and chrysin supplementation changed ER stress markers. However, aerobic exercise was most effective on ameliorating the high fat diet induced ER stress markers. Thus, it seems that aerobic exercise might have a more positive effect on skeletal muscle endoplasmic reticulum stress compared with polyphenol supplementation in high fat diet-induced obese mice.