• 제목/요약/키워드: IR laser

검색결과 200건 처리시간 0.028초

A Variety of Activation Methods Employed in “Activated-Ion” Electron Capture Dissociation Mass Spectrometry: A Test against Bovine Ubiquitin 7+ Ions

  • Oh, Han-Bin;McLafferty, Fred W.
    • Bulletin of the Korean Chemical Society
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    • 제27권3호
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    • pp.389-394
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    • 2006
  • Fragmentation efficiencies of various ‘activated-ion’ electron capture dissociation (AI-ECD) methods are compared for a model system of bovine ubiquitin 7+ cations. In AI-ECD studies, sufficient internal energy was given to protein cations prior to ECD application using IR laser radiation, collisions, blackbody radiation, or in-beam collisions, in turn. The added energy was utilized in increasing the population of the precursor ions with less intra-molecular noncovalent bonds or enhancing thermal fluctuations of the protein cations. Removal of noncovalent bonds resulted in extended structures, which are ECD friendly. Under their best conditions, a variety of activation methods showed a similar effectiveness in ECD fragmentation. In terms of the number of fragmented inter-residue bonds, IR laser/blackbody infrared radiation and ‘in-beam’ activation were almost equally efficient with ~70% sequence coverage, while collisions were less productive. In particular, ‘in-beam’ activation showed an excellent effectiveness in characterizing a pre-fractionated single kind of protein species. However, its inherent procedure did not allow for isolation of the protein cations of interest.

선택적 레이저 공정을 이용한 구리 나노 입자의 소결 특징 분석 및 플렉서블 전자 소자 제작 기술 개발에 관한 연구 (Study of Thermal Behaviors on sub-50 nm Copper Nanoparticles by Selective Laser Sintering Process for Flexible Applications)

  • 권진형;조현민;이하범;엄현진;고승환
    • 한국표면공학회:학술대회논문집
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    • 한국표면공학회 2016년도 추계학술대회 논문집
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    • pp.134-134
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    • 2016
  • The effect of different thermal treatments on the sub-50 nm copper nanoparticles is examined in the aspects of chemical, electrical and surface morphology. The copper nanoparticles are chemically synthesized and fabricated for paste-type solution. Simple bar coating method is practiced as a deposition process to form copper thin film on a typical slide glass. Deposited copper thin films are annealed by two different routes: general tube furnace with 99.99 % Ar atmosphere and selective laser sintering process. The thermal behavior of the different thermal-treated copper thin films is compared by SEM, XRD, FT-IR and XPS analysis. In this study, the laser sintering process ensures low annealing temperature, fast working speed and ambient-accessible route. Moreover, the laser-sintered copper thin film shows good electrical property and enhanced chemical stability than conventional thermal annealing process. Consequently, the proposed laser sintering process can be compatible with plastic substrate for flexible applications.

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온도센서를 이용한 CO2레이저 빔 조사면의 온도 분포 특성에 관한 연구 (The study on temperature distribution characteristic of irradiated surface by CO2 laser)

  • 민병대;김태균;정현주;김용철;정종한;김희제
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2003년도 하계학술대회 논문집 C
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    • pp.1831-1833
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    • 2003
  • Nowadays, CO2 lasers are used widely in many applications such as materials fabrication, communications, remote sensing and military purpose etc. Especially, CO2 lasers are in the spotlight at surface handling and heat processing. It is important to control the laser output power and beam quality in those fields. To increase beam qualify, We used the feedback system by various sensors. Although, CO2 lasers' output beam became feedback, its beam affected the irradiated material target already. Since, ideal real time control have still the problem to solve. Hence, we need the new proposal for more precise laser processing. So we expect the new effect how to change the irradiated material target as the kind of, processing time and output density caused by the CO2 laser beam. In this study, We have investigated the characteristics of the temperature and HAZ(Heat Affected Zone) by CO2 laser output with IR temperature sensor and RTD.

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Identification of a Marker Protein for Cardiac Ischemia and Reperfusion Injury by Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

  • Lee, Young-Suk;Kim, Na-Ri;Kim, Hyun-Ju;Joo, Hyun;Kim, Young-Nam;Jeong, Dae-Hoon;Cuong, Dang Van;Kim, Eui-Yong;Hur, Dae-Young;Park, Young-Shik;Hong, Yong-Geun;Lee, Sang-Kyung;Chung, Joon-Yong;Seog, Dae-Hyun;Han, Jin
    • The Korean Journal of Physiology and Pharmacology
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    • 제8권4호
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    • pp.207-211
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    • 2004
  • The purpose of the present study was to evaluate the expression of cardiac marker protein in rabbit cardiac tissue that was exposed to ischemic preconditioning (IPC), or ischemiareperfusion injury (IR) using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). We compared 2DE gels of control (uninjured) cardiac tissue with those of IPC and IR cardiac tissue. Expression of one protein was detected in IR heart tissue, however the protein was not detected in the samples of control and IPC tissue. To further characterize the detected protein molecule, the protein in the 2D gel was isolated and subjected to trypsin digestion, followed by MALDI-MS. The protein was identified as myoglobin, which was confirmed also by Western blot analysis. These results are consistent with previous studies of cardiac markers in ischemic hearts, indicating myoglobin as a suitable marker of myocardial injury. In addition, the present use of multiple techniques indicates that proteomic analysis is an appropriate means to identify cardiac markers in studies of IPC and IR.

Matrix Infrared Spectra and DFT Computations of 2H-Azirine Produced from Acetonitrile by Laser-Ablation Plume Radiation

  • Cho, Han-Gook
    • Bulletin of the Korean Chemical Society
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    • 제35권7호
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    • pp.2093-2096
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    • 2014
  • 2H-azirine, a less known acetonitrile isomer, is observed in matrix IR spectra from the precursor exposed to radiation from laser ablation of transition-metals. Its vibrational characteristics confirm the previous results, and those for the deuterated and $^{13}C$ substituted isotopomers are also newly reported. The weak absorptions are traced to the low production yield due to its high energy and low extinction constants. IRC computations reveal smooth inter-conversion between 2H-azirine and $CH_2NCH$, providing a rationale for the observed variation of their relative contents during photolysis.

펄스 레이저 애블레이션이 결합된 고전압 방전 플라즈마 장치를 이용한 유전성 질화탄소 박막의 합성 (Formation of dielectric carbon nitride thin films using a pulsed laser ablation combined with high voltage discharge plasma)

  • 김종일
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2003년도 하계학술대회 논문집 Vol.4 No.1
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    • pp.208-211
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    • 2003
  • The dielectric carbon nitride thin films were deposited onto Si(100) using a pulsed laser ablation of pure graphite target combined with a high voltage discharge plasma in nitrogen gas atmosphere. We can be calculated dielectric constant, ${\varepsilon}_s$, with a capacitance Sobering bridge method. We reported to investigate the influence of the laser ablation of graphite target and DC high voltage source for the plasma. The properties of the deposited carbon nitride thin films were influenced by the high voltage source during the film growth. Deposition rate of carbon nitride films were found to increase drastically with the increase of high voltage source. Infrared absorption clearly shows the existence of C=N bonds and $C{\equiv}N$ bonds. The carbon nitride thin films were observed crystalline phase, as confirmed by x-ray diffraction data.

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펄스 레이저 애블레이션이 결합된 고전압 방전 플라즈마 장치를 이용한 유전성 질화탄소 박막의 합성 (Formation of Dielectric Carbon Nitride Thin Films using a Pulsed Laser Ablation Combined with High Voltage Discharge Plasma)

  • 김종일
    • 한국전기전자재료학회논문지
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    • 제16권7호
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    • pp.641-646
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    • 2003
  • The dielectric carbon nitride thin films were deposited onto Si(100) substrate using a pulsed laser ablation of pure graphite target combined with a high voltage discharge plasma in the presence of a N$_2$ reactive gas. We calculated dielectric constant, $\varepsilon$$\_$s/, with a capacitance Schering bridge method. We investigated the influence of the laser ablation of graphite target and DC high voltage source for the plasma. The properties of the deposited carbon nitride thin films were influenced by the high voltage source during the film growth. Deposition rate of carbon nitride films were increased drastically with the increase of high voltage source. Infrared absorption clearly shows the existence of C=N bonds and C=N bonds. The carbon nitride thin films were observed crystalline phase confirmed by x-ray diffraction data.

스캐너와 산업용 로봇을 이용한 고속 레이저 용접에 관한 연구 (A Study on High Speed Laser Welding by using Scanner and Industrial Robot)

  • 강희신;서정;김종수;김정오;조택동
    • 대한용접접합학회:학술대회논문집
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    • 대한용접접합학회 2009년 추계학술발표대회
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    • pp.29-29
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    • 2009
  • On this research, laser welding technology for manufacturing automobile body is studied. Laser welding technology is one of the important technologies used in the manufacturing of lighter, safer automotive bodies at a high level of productivity; the leading automotive manufacturers have replaced spot welding with laser welding in the process of car body assembly. Korean auto manufacturers are developing and applying the laser welding technology using a high output power Nd:YAG laser and a 6-axes industrial robot. On the other hand, the robot-based remote laser welding system was equipped with a long focal laser scanner system in robotic end effect. Laser system, robot system, and scanner system are used for realizing the high speed laser welding system. The remote laser welding system and industrial robotic system are used to consist of robot-based remote laser welding system. The robot-based remote laser welding system is flexible and able to improve laser welding speed compared with traditional welding as spot welding and laser welding. The robot-based remote laser systems used in this study were Trumpf's 4kW Nd:YAG laser (HL4006D) and IPG's 1.6kW Fiber laser (YLR-1600), while the robot systems were of ABB's IRB6400R (payload:120kg) and Hyundai Heavy Industry's HX130-02 (payload:130kg). In addition, a study of quality evaluation and monitoring technology for the remote laser welding was conducted. The welding joints of steel plate and steel plate coated with zinc were butt and lapped joints. The quality testing of the laser welding was conducted by observing the shape of the beads on the plate and the cross-section of the welded parts, analyzing the results of mechanical tension test, and monitoring the plasma intensity and temperature by using UV and IR detectors. Over the past years, Trumf's 4kW Nd:YAG laser and ABB's IRB6400R robot system was used. Nowadays, the new laser source, robot and laser scanner system are used to increase the processing speed and to improve the efficiency of processes. This paper proposes the robot-based remote laser welding system as a means of resolving the limited welding speed and accuracy of conventional laser welding systems.

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광역 화재감지를 위한 적외선 레이저 연기 검출 시스템의 설계 및 구현 (A Design and Development of the Smoke Detection System Using Infra-red Laser for Fire Detection in the Wide Space)

  • 박장식;송종관;윤병우
    • 한국전자통신학회논문지
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    • 제8권6호
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    • pp.917-922
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    • 2013
  • 본 논문에서는 적외선과 가시광선 레이저를 이용하여 터널, 공항 등의 넓은 공간에서의 화재를 감지하기 위하여 연기검출 시스템을 제안한다. 제안하는 연기검출 시스템은 적외선 레이저 송수신기와 원거리에서 적외선 레이저 송수신 정합을 편리하게 하기 위한 가시광선 레이저 그리고 화재 경보를 전파하기 위한 Zigbee 무선통신 네트워크로 구성된다. 적외선 레이저 송신기와 수신기 사이에 연기가 발생하면 수신 신호의 크기가 기준값보다 작아지기는 것을 확인하고 연기를 검출한다. 적외선 레이저 송신기와 수신기 사이의 거리가 먼 경우에는 송신 측의 작은 변화에도 수신측에서는 상당히 큰 변이가 생겨 신호를 정합하는데 어려움이 있다. 본 논문에서 광역 연기검출을 위하여 적외선 레이저를 이용하고 효율적인 정합을 위하여 가시광선을 이용하는 방법을 제안한다. 그리고 연기가 감지되면 Zigbee 무선네트워크를 통하여 경보를 전달하는 연기검출 시스템을 개발하였다.

소아 암 환자에서 항암제 치료 후 발생한 구내염에 대한 저출력 레이저의 효과 (Effects of Low Level Laser Therapy on Oral Mucositis Caused by Anticancer Chemotherapy in Pediatric Patients)

  • 김혜자;노시연;신용섭
    • The Korean Journal of Pain
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    • 제14권1호
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    • pp.51-55
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    • 2001
  • Background: Oral mucositis is a common complication of anticancer chemotherapy. The sequelae of this consist of an increased risk of infection, moderate to severe pain, compromised oral function, and bleeding. This study was performed to evaluated the effects of the He-Ne laser and the Ga-Al-As laser on oral mucositis caused by anticancer chemotherapy in pediatric patients. Methods: There were 3 cases of osteosarcoma and 6 cases of leukemia. All patients received He-Ne laser (632.8 nm wavelength, power 60 mW) application on 400-600 Hz scanning for 5-20 minutes and Ga-Al-As laser (904 nm wavelength, power 40 mW) application by fiberoptic hand piece placed in immediate proximity to the tissue without direct contact with it for 30 seconds per point for 5 days per week. During the application patients wore wavelength-specific dark glasses and were instructed to keep their eyes closed. Results: The mean number of treatments with oral intake was $4.89{\pm}0.64$. The mean number of total treatments was $9.44{\pm}2.59$. There were no significant side effects during and after the laser treatments. Conclusions: He-Ne laser and Ga-Al-As (IR) laser treatment were well tolerated and reduced the severity and duration of chemotherapy-induced oral mucositis in pediatric oncologic patients.

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