• Molecular & Cellular Toxicology
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• v.1 no.1
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• pp.32-39
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• 2005

Polycyclic aromatic hydrocarbons (PAHs) are an important class of environmentally prevalent xenobiotics that exert complex effects on the biological system and characterized as probably carcinogenic materials. Single cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the T-and B lymphocytes, spleens (T/B-cell), bone marrow, and livers of mouse exposed to mixture of PAHs (Benzo(a)pyrene, Benzo(e)pyrene, Fluoranthene, Pyrene) at dose of 400, 800, or 1600 mg/kg body weight for 2 days. DNA damage of the cells purified from mice was increased in dose dependent manner. In the blood cells and organs, DNA damage was also discovered to vary directly with PAHs. Especially T-cells had been damaged more than B-cell. Plasma proteomes were separated by 2-dimensional electrophoresis with pH 4-7 ranges of IPG Dry strips and many proteins showed significant up-and -down expressions with the dose dependent manner. Of these, significant 4 spots were identified using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. Identified proteins were related to energy metabolism and signal transduction.

• The Optical Journal
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• s.153
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• pp.47-62
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• 2014

${\blacksquare}$ 목적 * 우리나라 주력산업분야(자동차, 조선, 항공, 기계, 로봇, 반도체 등)에 절단, 가공, 용접, 리소그라피, 마킹, 열처리, 합성, 표면처리 등의 방식으로 활용되고, 융 복합산업(의료, 에너지, 영상, 군수, 신소재, 센서)에 광원으로 응용되는 산업용 고출력 레이저의 산업 생태계 분석을 통하여 기존 산업의 고부가화와 신산업의 경쟁력을 견인 ${\blacksquare}$ 주요현황 * (시장현황) 2011년부터 2014년까지 고출력 레이저의 시장규모는 4,452백만불(5조 463억원)에서 5,255백만불(5조 9,565억원)으로 연평균 5.7% 성장 전망 * (제품기술동향) 3세대 레이저인 광섬유 레이저와 고출력 다이오드 레이저, DPSS 레이저 등이 기술혁신을 거듭하면서 1, 2세대 레이저를 대체해 가면서 높은 수준의 성장을 거듭할 것으로 전망. 산업용레이저의 주요선진국 대비 국내 기술수준은 전체적으로 44.5%정도로 취약 * (기업동향) Coherent, Trumpf, Cymer, IPG Photonics, Rofin-Sinar 5개사가 전체시장의 55%이상을 점유하고 있으며 대부분 수직계열화를 통해 경쟁력 확보. 최근 중국의 Han's 사가 자국정부의 지원을 통해 급 부상 ${\blacksquare}$ 시사점 * 산업용 레이저를 이용한 응용기술 부분 투자가 과거에는 단기 경제적 성취는 이로웠으나, 현재에 이르러 레이저 혹은 레이저 시스템이 필요할 때마다 외국에서 관련 핵심부품, 모듈 등을 수입해야하는 실정에 이르렀음. 향후 레이저 국산화를 위한 노력과 함께 후방 소재.부품의 전략적 투자를 통해 산업의 건강한 선순환생태 환경을 조성하여 산업 경쟁력을 갖추어야 함.

• Laser Solutions
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• v.11 no.4
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• pp.21-28
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• 2008

Remote Laser welding technology for manufacturing automotive body is studied. Laser welding and industrial robot systems are used for the robot based laser welding system. The laser system is used 1.6kW Fiber laser(YLR-1600) of IPG. The robot system is used HX130-02 of Hyundai Heavy Industry(payload : 130kg). The robot based laser welding system is equipped with laser scanner system for remote laser welding. The welding joints of steel plate and steel plate coated with zinc are butt and lapped joints. The quality test of the laser welding are through the observation the shape of bead on plate and cross-section of welding part. During past three years the laser system, 4kW Nd:YAG laser (HL4006D) of Trumpf was used and the robot system, IRB6400R of ABB (payload:120kg) was used. The new laser source, robot and laser scanner system are used to increase the processing speed and to improve the process efficiency. This paper introduces the robot based remote laser welding system to resolve the limited welding speed and accuracy of the conventional laser welding system.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09b
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• pp.1276-1277
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• 2006

The ever increasing requirements on today's compacts with regard to their geometry and precision call for flexible high-precision and most capable production systems. DORST Technologies has coped with these requirements by developing the new HP series for pressing forces between 1600 kN and 16000 kN and the new HS series for pressing forces between 150 kN and 1200 kN. These fully hydraulic presses featuring upper ram, lower ram, core rod, filler, up to 4 lower tool levels and up to 4 upper tool levels with closed-loop controlled movements. Thanks to latest servo technology and an electronic bus system it is possible to have all movements closed-loop controlled in the desired relation to each other. Thus, today's hydraulic presses provide high stroke rates, low energy consumption and a user-friendly interface. The input of data is carried out via clearly arranged screen masks on a touch-screen. The innovative DORST $IPG^{(R)}$ (Intelligent Program Generator) has been designed to support the set-up staff in preparing and optimizing the toolprogram. The combination of the machine type with the hydraulic unit determines the productivity in consideration of the specific application and the part to be pressed. Thanks to the closed-loop control circuits, DORST hydraulic automatic presses of the latest generation ensure unmatched precision and repeatability - and consequently process reliability - often without necessitating subsequent machining steps.

• Journal of Distribution Science
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• v.19 no.8
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• pp.101-112
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• 2021

Purpose: This study aims to explain the effect distribution strategy and competitiveness of entrepreneurial personality on marketing capabilities and performance, the effect of marketing capabilities on marketing performance, the role of marketing capabilities as a mediating variable on the effect of entrepreneurial personality on marketing performance, and the role of credit access moderating effect of entrepreneurial personality on marketing capabilities and performance. Research design, data, and methodology: This research was conducted in Bali, on a small clothing industry. The sample size was set at 150 respondents, using a cluster random sampling approach. Data were analyzed using descriptive analysis techniques and partial least square. Result: The findings show that; entrepreneurial personality and marketing capabilities have a significant effect on marketing performance, entrepreneurial personality has a significant effect on marketing capabilities. Another result shows that marketing capability mediates the effect of entrepreneurial personality on marketing performance, access to credit acts as a moderating predictor, and as quasi moderating the effect of entrepreneurial personality on marketing performance. Conclusions: Entrepreneurs should try to increase their knowledge and skills in marketing formally or informally. Competence in terms of individual knowledge and skills can indicate social capital investment, which indirectly contributes to one's mindset and insight.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.9
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• pp.1296-1302
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• 2004

This study was conducted to evaluate the possible application of 2 D-SDS-PAGE (2 DE)-based proteome analysis techniques to the assessment of extreme proteolysis in postmortem skeletal muscle. Eight Hanwoo longissimus muscles were incubated immediately after slaughter for 24 h at 5$^{\circ}C$, 15$^{\circ}C$ or 36$^{\circ}C$. Warner Bratzler (WB)-shear force and ultrastructural configuration were determined at 24 h, and rate of proteolysis to 24 h was determined by 1 D-SDS-PAGE (1 DE) and 2 DE. In addition, tentative protein identification was performed from peptide mass fingerprints of MALDI-ToF analysis of major protein groups on 2 DE profiles. The result showed that although ultrastructural configuration was similar between the 5$^{\circ}C$ and 36$^{\circ}C$ treatments, meat at 5$^{\circ}C$ had higher WBshear force (approximately 5 kg greater). A higher rate of protein degradation at 36$^{\circ}C$ was observed based on Troponin-T degradation, 1 DE, and 2 DE analysis. This indicates that proteolysis during the early postmortem period was a significant determinant of shear force at 24 h. Little difference in proteolysis between 5$^{\circ}C$ and 15$^{\circ}C$ treatments was found based on classic 1 DE profile assessment. Meanwhile, considerable differences in the 2 DE profiles between the two treatments were revealed, with substantially higher rate of proteolysis at 15$^{\circ}C$ compared to 5$^{\circ}C$. Nuclease treatment improved 2 DE profile resolution. 400 ${\mu}$g and 600 ${\mu}$g of sample loading appeared to be appropriate for 24 cm pH 3-10 and pH 5-7 IPG strips, respectively. Protein detection and quantification of the 5$^{\circ}C$, 15$^{\circ}C$ and 36$^{\circ}C$ 2 DE profiles revealed 78, 163 and 232 protein spots respectively that were differentially modified in terms of their electrophoretic properties between approximately pI 5.3-7.7 with the molecular weight range of approximately 71-12 kDa. The current results demonstrated that 2 DE was a superior tool to 1 DE for characterising proteolysis in postmortem skeletal muscle.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.90-90
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• 2003

체세포 핵이식에 의한 복제기술은 매우 낮은 성공률 나타내고 있어 실용화에 지장을 초래하고 있다. 이것은 후생적인 유전현상인 reprogramming이 불완전하게 이루어지기 때문인 것으로 추측되어지고 있다(Reik et al., Theriogenology 2003, 59: 21-32; Han et al, Theriogenology 2003, 59: 33-44). 체세포 핵이식 후에 태아사망의 원인이 태반의 비정상적인 기능과 관계가 있는 것으로 추정되는데 복제시 태아사망의 원인을 찾기 위해 본 연구를 시행하였다. 한우에서 체세포 복제 후 임신 말기에 태아가 사망한 태반조직 3개와 IVF 수정란 이식 후 동일한 시기에 제왕절개술을 실시한 태반조직 2개를 실험에 이용하였다. 태반 protein을 Two-Dimensional electrophoresis와 Mass spectrometer를 이용하여 분석 비교하였다. IPG-system을 이용하여 pH 4~7, pH 6~9에서 1차 전기영동을 한 후, 8~l6%의 SDS-PAGE gel에 2차 전기영동을 실시하였고 G-250 Coomassie로 염색하였다. gel 이미지는 Malanie III program을 이용하여 분석하였다. 전체 gel에서 약 1800개의 구분 가능한 protein spot이 나타났다. pH 4~7 범위에서 양적으로 차이나는 것 15개 중 복제한우 태반에서 증가되는 protein spot 5개와 감소하는 protein spot 10개를 골라 protein identification을 실시하였다. MALDI-TOF-MS를 이용하여 동정한 결과 phosphatidylinositol transfer protein-$\alpha$와 interleukin-18 등의 protein이 복제태반에서 발현이 증가되었고, 복제한우에서 발현이 감소되는 것으로는 vimentin, Rho-GDI-$\beta$, TRAST $\beta$-chain, ovarian sterol carrier protein 2, triosephosphate isomerase, tropemyosin beta chain, Aldose reductase 등으로 나타났다. 이러한 protein들은 inositol 지질 신호전달과 면역시스템, 세포분열, 산소 운반, steroidogenic 세포에서의 콜레스테롤 이동, 촉매 작용, 대사 작용 등에 중요한 역할을 하는 것으로 알려져 체세포 복제에 의한 태아사망 원인은 태반에서 이러한 protein들의 비정상적인 발현에 기인된 것으로 추정된다.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.171-178
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• 2017

A series of novel effector molecules secreted by the type three secretion system (T3SS) of Shigella spp. have been reported in recent years. In this study, a proteomic approach was applied to study T3SS effectors systematically. First, proteins secreted by the S. flexneri wild-type strain after Congo Red induction were separated and identified using two-dimensional electrophoresis to display the relative abundance of all kinds of early effectors for the first time. Then, a gene deletion mutant of known virulence repressor (OspD1) and a gene overexpressed mutant of two known virulence activators (MxiE and IpgC) were constructed and analyzed to discover potential late effectors. Furthermore, the supernatant proteins of gene deletion mutants of two known translocators (IpaB and IpaD), which would constantly secrete effectors, were also analyzed. Among all of the secreted proteins identified in our study, IpaH1.4, IpaH_5, and IpaH_7 have not been reported before. These proteomics data of the secreted effectors will be valuable to understand the pathogenesis of S. flexneri.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.85-85
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• 2003

한우 난소의 황체는 다양한 세포들로 구성되어 있으며 난소의 생식기능유지와 임신유지에 중요한 인자가 복잡하게 관련되어 있으며 이들 황체에서 분비하는 단백질은 황체기능에 필수적으로 중요한 작용을 한다. 본 연구는 난소의 황체일령에 따른 단백질 분비 패턴을 조사함으로써 황체세포의 기능과 임신 유지에 관련되는 인자들을 조사하기 위하여 수행하였다. 한우에서 채취한 난소에서 황체를 분리, 황체시기별(전기, 중기, 말기)로 구분하여 cytosol을 분리 정제하였다. 황체시기별로 분리된 황체 단백질의 성분을 분석하기 위해 ion-exchange chromatography를 이용하여 단백질 패턴을 조사, 추출된 fraction을 단백질 정량후 SDS-PAGE를 실시하였다. 그 결과 중기에서의 단백질의 농도가 가장 높았으며, 특히 단백질 패턴 또한 다른 양상을 보였다. 시기별로 구분한 각각의 fraction을 SDS-PAGE로 조사했을 때 중기와 말기황체 cytosol의 fraction 3번과 4번에서 다른 양상을 보였으며 SDS-PAGE에서 120kb, 95kb, 34kb, 25kb 등의 단백질 밴드를 확인할 수 있었다. 초, 중기황체에서만 특이하게 검출되는 단백질 band를 확인할 수 있었으며 이 들 단백질을 구체적으로 확인하기 위하여 Two-Dimensional electrophoresis를 이용하여 실험을 수행하였다. 시기별로 분리된 황체를 일반적인 IEF단백질 분리법으로 cytosol을 회수한 후 IPG-system을 이용하여 1차원 전기영동을 한 후, SDS-PAGE로 이차원 전기영동을 실시하였다. 이차원 전기영동 결과, SDS-PAGE의 결과와 비슷한 위치에 부분적으로 다른 spot의 양상을 보였다. 특히 기능황체에서의 특이적 발현 spot을 확인할 수 있었다. 이러한 결과들로부터 황체의 progesterone분비기능의 역할을 수행하기 위한 단백질들이 전, 중기에 발현된다는 것을 알 수 있고 퇴행황체에서는 발현이 안되고 있는 것을 알 수 있다. 이러한 결과를 토대로 다른 양상을 띤 spot을 분리하여 어떤 단백질인지를 분석하여 각각의 황체단백질의 특성을 규명할 수 있을 것으로 사료된다.

• Transactions of the Korean Society of Automotive Engineers
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• v.22 no.3
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• pp.250-256
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• 2014

EV(Electric Vehicle) has many benefits such as prevention of global warming and so on. But due to driving source changing from combustion engine to battery and e-motor, new R&D difficulties have arisen which changing of desired vehicle performance and multidisciplinary design constraints by means of strong coupled multi-physics domain problems. Additionally, dynamics performances of EV becomes more important due to increasing customer's demands and expectations for EV in compare with internal combustion engine vehicle. In this paper suggests model based development platform of EV through integrated simulation environment for improving analyse & design accuracy in order to solve multi-physics problem. This simulation environment is integrated by three following specialized simulation tools IPG CarMaker, AVL Cruise, DYMOLA that adapted to each purpose. Furthermore, control algorithm of TV(Torque Vectoring) system is developed using independent driven e-motor at rear wheels for improving handling performance of EV. TV control algorithm and its improved vehicle performances are evaluated by numerical simulation from standard test methods.