• Journal of Plant Biotechnology
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• 제3권2호
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• pp.67-81
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• 2001

Traditionally, genetic variability is generated by an extensive crossing program, which is complemented by strict selection to identify useful new recombinants. Plant biotechnology offers many opportunities for breeders to solve certain breeding problems at the molecular level. The tissue culture methodology and the genetic modification of economically important monocotyledons have undergone a revolution in the last decade. As the production of transgenic plants is a complex procedure, including the uptake of DNA molecules into the cells, the integration of foreign nucleotide sequences into the host genomic DNA and the expression of new genes in a controlled way, and as there are still many unsolved questions, further development is necessary. The methodology opens up the possibility of introducing novel genes that may induce resistance to diseases and abiotic stresses, allow the modification of dough quality and the dietetic quality of proteins, and increase the levels of micronutrients such as iron, zinc, and vitamins. In the present review, the authors would like to summarise the most important advances in wheat transformation.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.366-372
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• 2009

This study was conducted to develop new lines expressing the characteristic of early flowering by introducing MdMADS2 gene in chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) ‘Zinba'. Transformation of chrysanthemum was conducted by Agrobacterium tumefaciens LBA4404 harboring the binary vector containing MdMADS2 controlled by double CaMV 35S promoters. Ninety three shoots were regenerated from 1,463 leaf segment explants cultured on the first selection medium (MS basal salts + 1.0 mg/L BA + 0.5 mg/L IAA + 10 mg/L kanamycin + 400 mg/L cefotaxime, pH 5.8) after co-cultivation, and 20 out of the 93 shoots rooted on the second selection medium containing 20 mg/L kanamycin and 400 mg/L cefotaxime. Many escapes (98.6%) were removed on the selection stage for rooting. Nineteen lines were confirmed as transgenic plant with transgene by PCR analysis. Six transgenic plants flowered 2-11 days earlier than non-transgenic plant without big change of phenotype, and especially, 3 (Mo-7, Mo-11, Mo-17) out of 6 transgenic lines showed a significant reduction in days to flowering compared to non-transgenic plant. Introduction and expression of MdMADS2 gene in them were confirmed by Southern and real-time PCR analyses, respectively.

• Journal of Applied Biological Chemistry
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• 제49권3호
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• pp.75-81
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• 2006

A full-length cDNA encoding dihydroflavonol 4-reductase (st-dfr) of potato was isolated by rapid amplification of cDNA ends, and their expression was investigated from purple-fleshed potato (Solanum tuberosum L. cv. Jashim). The st-dfr exists as a member of a small gene family and its transcripts was abundant in the order of tuber flesh, stem, leaf, and root. The expressions of st-dfr gene were light inducible and cultivar dependant. Transgenic potato plants harboring antisense st-dfr (AS-DFR) sequences were analyzed. The accumulation of mRNA was nearly completely inhibited as a result of introducing an AS-DFR gene under the control of the 35S CaMV promoter into the red tuber skin Solanum tuberosum L. cv. Desiree. The anthocyanin content of the tuber peels of the transgenic lines was dramatically decreased by up to 70%. The possible production of flavonols in the peels of AS-DFR transgenic potatoes was discussed.

• 한국연초학회지
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• 제11권1호
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• pp.41-48
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• 1989

The significance of soil and/or rhisosphere populations of Erwinia carotovora sobsp. carotovora (Ecc) as a source of primary inoculum for tobacco hollow stalk disease has been demonstrated conclusively. The survival of Ecc in field soils fter overwintering was estimated by using the enrichment technique. The population number of pectolytic erwinia (PE) in field soils relatively decreased at the rate of 102-104 colony forming unit(CFU) per g of soil after overwintering. Higher level of PE population overwintered in the rhlzosphere foils of tobacco stubbles and detected more frequently in rhizosphere soils of weed plants than in those of bare fields. All of the tobacco stubbles collected from fields where tobacco had been grown the previous year contained Ecc. The more survived population number of PE at the 30cm depth of artifitiany infested soils than at the upper of those by introducing with diseased tobacco plant tissue after overwintering. Ecc overwintered effectively in rhizosphere soils of tobacco stubbles, overwintered weeds and tobacco debris in field soils.

• Nuclear Engineering and Technology
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• 제37권2호
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• pp.201-206
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• 2005

Fretting, which is a special type of wear, is defined as small amplitude relative motion along the contacting interface between two materials. The structural integrity of steam generators in nuclear power plants is very much dependent upon the fretting wear characteristics of Inconel 690 U-tubes. In this study, a finite element model that can simulate fretting wear on the secondary side of the steam generator was developed and used for a quantitative investigation of the fretting wear phenomenon. Finite element modeling of elastic contact wear problems was performed to demonstrate the feasibility of applying the finite element method to fretting wear problems. The elastic beam problem, with existing solutions, is treated as a numerical example. By introducing a control parameter s, which scaled up the wear constant and scaled down the cycle numbers, the algorithm was shown to greatly reduce the time required for the analysis. The work rate model was adopted in the wear model. In the three-dimensional finite element analysis, a quarterly symmetric model was used to simulate cross tubes contacting at right angles. The wear constant of Inconel 690 in the work rate model was taken as $K=26.7{\times}10^{-15}\;Pa^{-1}$ from experimental data obtained using a fretting wear test rig with a piezoelectric actuator. The analyses revealed donut-shaped wear along the contacting boundary, which is a typical feature of fretting wear.

• 한국식품영양학회지
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• 제28권2호
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• pp.202-218
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• 2015

Many countries have introduced new imported food safety measures, following the accident at Fukushima Daiichi Nuclear Power Station. This study was conducted to evaluate the measures contents and effects on food trades values. Eight percent of members were notified the introduced measures to the World Trade Organization. The measures' contents were banning imports, enhancing inspection and adding certification requirement. The covered regions were some prefectures, entire Japan or all affected countries. European Union introduced a measure that subjecting foods originating from 12 prefectures to import at designated ports with required certification. The measures were amended 8 times until March 2014 to apply listed foods from 15 prefectures. The trade value of fishery products and miscellaneous foods were affected. Australia introduced a measure that required additional inspection of dairy, fishery and plants products from 13 prefectures with subsequent amendments. The trade value had no effect in tested foods. Chinese Taipei introduced a temporary import ban for all foods from 6 prefectures. Trade values for fruits were affected. The United States issued an import alert for detention without examination for listed prefectures and goods without introducing new measures. Although no specific products were affected, trade values for all foods were affected.

• Nuclear Engineering and Technology
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• 제47권7호
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• pp.884-894
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• 2015

Background: Fibers in concrete resist the growth of cracks and enhance the postcracking behavior of structures. The addition of fibers into a conventional reinforced concrete can improve the structural and functional performance of safety-related concrete structures in nuclear power plants. Methods: The influence of fibers on the ultimate internal pressure capacity of a prestressed concrete containment vessel (PCCV) was investigated through a comparison of the ultimate pressure capacities between conventional and fiber-reinforced PCCVs. Steel and polyamide fibers were used. The tension behaviors of conventional concrete and fiber-reinforced concrete specimens were investigated through uniaxial tension tests and their tension-stiffening models were obtained. Results: For a PCCV reinforced with 1% volume hooked-end steel fiber, the ultimate pressure capacity increased by approximately 12% in comparison with that for a conventional PCCV. For a PCCV reinforced with 1.5% volume polyamide fiber, an increase of approximately 3% was estimated for the ultimate pressure capacity. Conclusion: The ultimate pressure capacity can be greatly improved by introducing steel and polyamide fibers in a conventional reinforced concrete. Steel fibers are more effective at enhancing the containment performance of a PCCV than polyamide fibers. The fiber reinforcementwas shown to bemore effective at a high pressure loading and a lowprestress level.

• 상하수도학회지
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• 제32권6호
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• pp.517-526
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• 2018

Climate adaptation strategies for water utilities including 16 water treatment plants(WTPs) in Jeju were investigated. Drought, heat wave, and heavy rain were among the most significant climate factors affecting water utilities in Jeju. Heat wave increases water temperature, which in turn increases the concentration of algae, color, and odor materials. Some adaption strategies for the heat wave can be strengthening water monitoring and introducing advanced water treatments. Heavy rain increases raw water turbidity in surface water. The 7 WTPs that take raw water from streams or springs had a maximum turbidity of less than 50 NTU under heavy rain. However, due to concerns of turbidity spike in treated water, some WTPs discontinued intaking raw water when raw water turbidity increased more than 2 NTU. They instead received treated water from other WTPs which took groundwater for water supply. This happens because of the low skills of employees. Thus, there needs to be an increase in operator competency and upgrade of water facilities for the adaption of heavy rain. To improve adaption for the drought, there should be an increase in the capacity of intake facilities of surface water as well as a decrease in water loss. In addition, water consumption per person should be decreased.

• 신재생에너지
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• 제17권4호
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• pp.9-27
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• 2021

Floating solar power markets are rapidly growing worldwide. The main policy instrument utilized to expand renewable energy use in foreign countries with many floating solar power installations is Feed-in-Tariffs (FITs). Foreign countries apply FIT to projects that have a secured grid connection, and lately, there has been a change in the direction of introducing or expanding auction systems. Vietnam and Taiwan give higher FIT to floating solar installations than land solar ones, and China, Vietnam, and Taiwan have higher FITs for certain regions. Compared to foreign countries where large-scale floating solar power installations have been installed, Korea has utilized Renewable Energy Certificate (REC) weights for residents' participation are provided additionally under the Renewable Portfolio Standard (RPS). In contrast to Korea, where residents' participation and benefit profit sharing are emphasized, the Netherlands provides opportunities for local residents to participate in floating solar power projects through cooperatives to improve the residents' acceptance.

• The Plant Pathology Journal
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• 제39권3호
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• pp.303-308
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• 2023

The global climate change and international trade have facilitated the movement of plants across borders, increasing the risk of introducing novel plant viruses in new territories. Ixora coccinea exhibited virus-like foliar symptoms, including mosaic and mild mottle. An Oxford Nanopore Technologies-based compact and portable MinION platform was used to identify the causal viral pathogen. The complete genome sequence of jasmine virus H (JaVH; 3867 nt, JaVH-CNU) was determined and found to share 88.4-90.3% nucleotide identity with that of Jasminum sambac JaVH isolate in China. Phylogenetic analysis based on the complete amino acid sequences of RNA-dependent RNA polymerase and coat protein revealed that JaVH-CNU was grouped separately with other JaVH isolates. This is the first report of a natural JaVH infection of I. coccinea. The application of rapid nanopore sequencing for plant virus identification was demonstrated and is expected to provide accurate and rapid diagnosis for virus surveillance.