• 한국식물병리학회지
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• 제11권4호
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• pp.374-379
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• 1995

The cDNA of tobacco mosaic virus-pepper strain (TMV-P) coat protein (CP) genes were introduced into tobacco plants (Nicotiana tabacum cv. Samsun nn) using a binary Ti plasmid vector of Agrobacterium tumefaciens. these cDNAs introduced into tobacco plants were detected by polymerase chain reaction. Symptom development was distinctly suppressed in the transgenic plant introduced buy sense CP cDNA when the plant was inoculated with TMV-P, while in transgenic tobacco plants of antisense CP gene, symptom development was not suppressed as in non-transgenic plants. TMV-P concentration in the sense CP transgenic tobacco plant was decreased to 1/14 of the concentration in non-transgenic plants. Expression of the kanamycin resistance gene of these transgenic plants could be detected in the progeny.

• 한국식물병리학회지
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• 제11권1호
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• pp.66-72
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• 1995

The coat protein (CP) gene of cucumber mosaic virus-As (CMV-As) strain was engineered for expression in the plant by using the cauliflower mosaic virus 35S transcript regulatory sequences. The CP gene was cloned into an Agrobacterium-derived binary vector. A chimeric gene was constructed by the cDNA of CMV-As CP and plant expression vector pBI121. The clone, pCMAS66, was first introduced into the phagemid vector pSPORT1 for situating sense orientation for translation and making restriction sites in order to re-introduce plant expression vector, pHI121. The resulting subclone pCASCP02 and plant expression vector pBI121 were treated with BamHI-SacI for excising the target gene and removing GUS gene, respectively. After Agrobacterium transformation by freeze-thaw technique, the clone, pCMASCP121-123 which contains sense orientation of the target gene, was selected and confirmed by restriction endonuclease analysis. The CMV-As CP gene was introduced into A. tumefaciens. The results on tobacco plant transformation with the vector system revealed that the system could be successfully introduced and showed high frequency of selection to putative transformations.

• The Korean Journal of Ecology
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• 제21권5_1호
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• pp.457.2-486
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• 1998

Ecological success of introduced species is frequently attributed to the reproductive characters of those species. We examined the relationship between both flowering season and plant size and reproductive characters in two introduced species of Taraxacum, e.g., T. officinale and T.laevigatum. Seventy six plants of T. officinale and 23 plants of T. laevigatum were randomly tagged from a population at the sungshin Women's University in April of 1997. The size and number of vegetative characters such as rosette diameter, leaf number, and the largest leaf length and width were measured for each plant at the onset of flowering. The infructescence was collected continually from each plant before seed dispersal from April to August of 1997. The number of infructescence per plant, and seed number and total seed weight per infructescence were measured. Mean individual seed weight per infructescence was also obtained. T. officinale possessed larger sized, but smaller number of, leaves than T. laevigatum, thereby resulting in no significant difference in total leaf area. There was a trend that vegetatively larger plants in both of these species produced reproductive characters larger in size or greater in number, except for seed number and total seed weight per infructescence in T. laevigatum. All reproductive characters examined in T. officinale decreased toward the end of flowering season. In T. laevigatum, infructescence number per plant also declined during a flowering season: however, mean seed number per infructescence increaed toward the end of flowering season with no significant seasonal change in the total seed weight per infructescence. T. officinale produced on average about twice as many seeds per plant as T. laevigatum during a growing season. These two Taraxacum species produced quite small seeds in size compared to the endemic species, exhibiting a far better dispersal ability of introduced species. These data demonstrate that most reproductive characters decline in size and number in both species during a long flowering period, but the two species appear to employ different strategies to achieve reproductive success in disturbed habitats.

• 식물분류학회지
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• 제51권4호
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• pp.386-390
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• 2021

Petrorhagia nanteuilii (Burnat) P. W. Ball & Heywood (Caryophyllaceae), native to western Europe and western North Africa and an introduced or alien plant in South America, Australia, and Japan, was newly found in Gyeongsangnam-do, Korea. Petrorhagia is clearly distinguished from other genera by a bract at the base of calyx, which differs from Gypsophila L., and commissures at the sepals, which is different from Dianthus L. Petrorhagia nanteuilii grows on slopes and roadsides, suggesting that it is likely to have been introduced through the installation of green sites and road construction. A precise description, illustration, photographs, and a key to related genera and species is provided.

• The Plant Pathology Journal
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• 제30권4호
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• pp.331-342
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• 2014

Given the lack of a resistant genetic pool in host plants, the introduction of exotic invasive pathogens can result in epidemics that affect a specific ecosystem and economy. Plant quarantine, which is designed to protect endemic plant resources, is a highly invaluable safeguard that should keep biosecurity with increasing international trade and global transportation. A total of 34 species of plant pathogens including Phytophthora infestans were documented as introduced from other countries into Korea from 1900 to 2010. The genus Phytophthora, classified in oomycetes, includes more than 120 species that are mostly recognized worldwide as highly invasive plant pathogens. After 2000, over 50 new species of Phytophthora were identified internationally as plant pathogens occurring in crops and forest trees. In Korea, Phytophthora is also one of the most serious plant pathogens. To date, 22 species (about one-fifth of known species) of the genus have been identified and reported as plant pathogens in the country. The likelihood of new exotic Phytophthora species being introduced into Korea continues to increase, thus necessitating intensive plant quarantine inspections. As new potential threats to plant health in Korea, six Phytophthora species, namely, P. alni, P. inundata, P. kernoviae, P. pinifolia, P. quercina, and P. ramorum, are discussed in this review with focus on history, disease, biology, management, and plant quarantine issues.

• Journal of Veterinary Science
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• 제24권1호
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• pp.5.1-5.16
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• 2023

The H9N2 avian influenza (AI) has become endemic in poultry in many countries since the 1990s, which has caused considerable economic losses in the poultry industry. Considering the long history of the low pathogenicity H9N2 AI in many countries, once H9N2 AI is introduced, it is more difficult to eradicate than high pathogenicity AI. Various preventive measures and strategies, including vaccination and active national surveillance, have been used to control the Y439 lineage of H9N2 AI in South Korea, but it took a long time for the H9N2 virus to disappear from the fields. By contrast, the novel Y280 lineage of H9N2 AI was introduced in June 2020 and has spread nationwide. This study reviews the history, genetic and pathogenic characteristics, and control strategies for Korean H9N2 AI. This review may provide some clues for establishing control strategies for endemic AIV and a newly introduced Y280 lineage of H9N2 AI in South Korea.

• 농업과학연구
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• 제3권1호
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• pp.121-134
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• 1976

1) 우리나라가 외국(外國)과 식물(植物), 농산물무역(農産物貿易)을 시작한 20세기초이후(世紀初以後) 현재(現在)까지 여러가지 식물병원균(植物病原菌)이 수입식물(輸入植物), 농산물(農産物)과 함께 국내(國內)에 침입정착(侵入定着)하여 피해(被害)를 준 증거(證據)가 있다. 2) 외국(外國)의 식물병원균(植物病原菌)이 국내(國內)에 침입(侵入)하는 경로(經路)는 여러가지 있으나 주(主)로 수입(輸入)하는 식물(植物), 농산물(農産物)에 잠복(潛伏), 부착(附着)하여 침입(侵入)한다. 3) 식물병원균(植物病原菌)의 국내침입(國內侵入)을 효과적(效果的)으로 방지(防止)할수 있는 식물검역제도(植物檢疫制度)가 우리나라에서 처음으로 시행(施行)된 것은 1912년(年)이며 세계식물검역사(世界植物檢疫史)에서 볼때 그 시행(施行)이 비교적(比較的) 일은 국가(國家) 크릅에 속(屬)한다. 4) 식물검역제도(植物檢疫制度)가 시행(施行)된 다음에도 여러가지 식물병원균(植物病原菌)이 침입(侵入)한 증거(證據)가 있다. 특히 2차세계대전(次世界大戰) 이후(以後) 1961년(年) 식물검역법(植物檢疫法)이 제정(制定)되어 식물검역(植物檢疫)이 재개(再開)될때까지의 약(約)15년간(年問)은 식물검역(植物檢疫)의 공백기(空白期)로서 여러가지 식물병원균(植物病原菌)이 국내(國內)에 침입(侵入)할수 있는 좋은 기회(機會)가 되었다. 5) 국내(國內)에 침입(侵入)한 식물병원균(植物病原菌)의 대다수(大多數)가 일본에서 유래(由來)한 것이며 이는 우리나라의 식물(植物), 농산물(農産物)의 무역(貿易)이 일본에 편중(偏重)되어 있었다는데 기인(起因)하는 것으로 추정(推定)된다. 6) 오늘날의 식물검역(植物檢疫)에는 더욱 정확성(正確性)과 지속성(遲速性)이 요구(要求)되므로 훈련(訓練)된 전문직검역관(專問職檢疫官)의 증원(增員) 대량(大量)의 식물(植物) 농산물(農産物)은 수용검역(收容檢疫)할수 있는 시설(施設)의 확보(確保)등 검역체제(檢疫體制)의 정비(整備)와 새로운 검역기술(檢疫技術)의 도입(導入), 개발(開發)등이 필요(必要)하다. 7) 식물검역(植物檢疫)의 체제정비(體制整備)와 기술향상(技術向上)은 국내(國內)에 침입(侵入)하는 식물병원균(植物病原菌)을 효과적(效果的)으로 방지(防止)하여 국내농업(國內農業)을 보호(保護)하는데 중요(重要)한 것 일뿐만 아니라 우리나라 식물농산물수출(植物農産物輸出)의 증대(增大)와 안정화(安定化)를 위하여도 중요(重要)한 일이다.

• Journal of Applied Biological Chemistry
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• 제42권3호
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• pp.113-118
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• 1999

The transgenic plant of Citrus species (Citrus aurantium L.) was constructed with a fatty acid desaturase gene using microprojectile bombardment transformation system. The DNA of a fatty acid desaturase gene, fad7, constructed in pBI121 was coated onto tungsten particles ($1.1{\mu}m$) and introduced into callus cells by bombarding with 1100 psi of helium pressure, 1/4 in of gap distance, 7.0 cm of target distance and 27 in Hg of chamber vacuum. The bombarded cells were selected on the medium containing kanamycin. The selected cells were successfully regenerated into plantlets via somatic embryogenesis on the media containing plant growth regulators. The results of polymerase chain reaction analysis of genomic DNAs from the putative transformants showed that the introduced DNAs of fad7 were present in both the selected callus cells and the regenerated plantlets.

• 시스템엔지니어링학술지
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• 제12권1호
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• pp.7-23
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• 2016

Plant engineering industry is considered as a key industry which will drive the future of Korea. However, Korean plant engineering companies have recently made huge losses in overseas businesses and the lack of engineering capability is pointed out as a main cause of this situation. Unlike Korean plant engineering companies, world leading engineering companies such as Flour and Bechtel have their own systems Engineering Standards/Guides ensuring successful fulfillments of the concept and basic design processes. An engineering standard for an organization is an essential means to shorten the time for engineering design, to maintain the engineering quality and to secure the engineering efficiency in the development of the complex system. Korean plant engineering companies'lack of engineering capability comes from the absence of the engineering standard. In the paper, we have developed a steel-making plant engineering standard based on a systems engineering standard. We chose both ISO/IEC/IEEE 15288 and NASA SE Handbook as main reference standards. First, we have introduced a life-cycle definition and a physical hierarchy of a general steel-making plant. Then we have introduced detailed engineering processes of each life-cycle stage. The full scope of the study was from the feasibility study to the basic design but in the paper, we have only introduced detailed engineering processes and exit criteria for the feasibility study and the concept design.

• The Plant Pathology Journal
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• 제17권1호
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• pp.1-8
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• 2001

The technique of plant transformation has started to show off its great power in the area of plant breeding by commercially successful introduction of transgenic varieties such as herbicide tolerant soybean and insect resistant corn in USA with an unimaginable speed. However, in contrast with the great success in the commercialization of herbicide tolerance and insect resistance, the transformation works on disease resistance has not yet reached the stage of full commercialization. This review surveys the current status of molecular breeding for plant disease resistance and their limits and problems. Some novel ideas and approaches in molecular breeding for disease resistance are introduced.