• Allergy, Asthma & Immunology Research
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• 제10권6호
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• pp.662-674
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• 2018

Purpose: Group 2 innate lymphoid cells (ILC2s) have been implicated in the pathogenesis of allergic disease. However, the effect of allergen-specific immunotherapy (AIT) on ILCs remains to be clarified. The aim of this study was to evaluate the levels of ILC subsets in allergic rhinitis (AR) patients in response to house dust mite (HDM)-specific immunotherapy. Methods: We enrolled 37 AR patients undergoing AIT (16 responders and 11 non-responders) for 2 years, 35 HDM AR patients and 28 healthy subjects. Peripheral blood mononuclear cells (PBMCs) were analyzed by flow cytometry to identify ILC subsets. Stimulation of ILC2s with recombinant allergen-specific protein was used to determine ILC2's activation (CD69 expression). Results: Responder AIT patients and healthy subjects had a decreased frequency of circulating ILC2s compared to non-responder AIT and AR patients. Conversely, ILC1s from responder AIT patients and healthy subjects showed increased frequency compared to non-responder AIT and AR patients. The frequency of ILC3s natural cytotoxicity receptor $(NCR)^+$ and $NCR^-$ in responder AIT patients was significantly lower compared to AR patients and healthy subjects. The ILC1: ILC2 proportion in responder AIT patients was similar to that of healthy subjects. PBMCs from patients who were responders to AIT had a significantly lower expression of the activation marker CD69 on ILC2s in response to allergen re-stimulation compared to AR patients, but no difference compared to non-responder AIT patients and healthy subjects. Conclusions: We propose that AIT might affect ILC responses. The activation of ILC2s was reduced in AR patients treated with AIT. Our results indicate that a relative ILC1/ILC2 skewed response is a possible key to successful AIT.

• 대한의생명과학회지
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• 제23권4호
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• pp.339-347
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• 2017

This study was performed to evaluate the cytotoxicity induced by ischemia-like condition (ILC) in cultured neuroglial cells (C6 glioma cells). The protective effect of kaempferol (KAE), flavonoid against the cytotoxicity induced by ILC induction was assessed. In addition, antioxidative effects of KAE were done by colorimetric assays. Cell viability and the antioxidative effects such as DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and inhibitory activity of lipid peroxidation (LP) were analyzed. ILC induction decreased cell viability in a dose-dependent manner, and the $XTT_{90}$ value (low cytotoxicity value) and $XTT_{50}$ value (high cytotoxicity value) were determined during ILC induction for 15 and 40 minutes, respectively. The butylated hydroxytoluene (BHT) antioxidant significantly increased cell viability damaged by the ILC-induced cytotoxicity. In the protective effect of KAE on ILC-induced cytotoxicity, KAE protected the ILC-induced cytotoxicity by the significant increase of cell viability, and also it showed DPPH-radical scavenging ability, SOD-like ability and inhibitory ability of LP. From these results, it is suggested that ILC induction showed cytotoxicity in these cultures and the oxidative stress is involved in the ILC-induced cytotoxicity. While, KAE prevented ILC-induced cytotoxicity by antioxidative effects. In conclusion, natural products like KAE may be a putative therapeutic agent for the treatment of disease associated with oxidative stress such as ischemia.

• IMMUNE NETWORK
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• 제23권2호
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• pp.15.1-15.17
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• 2023

Innate lymphoid cells (ILCs) are critical immune-response mediators. Although they largely reside in mucosal tissues, the kidney also bears substantial numbers. Nevertheless, kidney ILC biology is poorly understood. BALB/c and C57BL/6 mice are known to display type-2 and type-1 skewed immune responses, respectively, but it is unclear whether this extends to ILCs. We show here that indeed, BALB/c mice have higher total ILCs in the kidney than C57BL/6 mice. This difference was particularly pronounced for ILC2s. We then showed that three factors contributed to the higher ILC2s in the BALB/c kidney. First, BALB/c mice demonstrated higher numbers of ILC precursors in the bone marrow. Second, transcriptome analysis showed that compared to C57BL/6 kidneys, the BALB/c kidneys associated with significantly higher IL-2 responses. Quantitative RT-PCR also showed that compared to C57BL/6 kidneys, the BALB/c kidneys expressed higher levels of IL-2 and other cytokines known to promote ILC2 proliferation and/or survival (IL-7, IL-33, and thymic stromal lymphopoietin). Third, the BALB/c kidney ILC2s may be more sensitive to the environmental signals than C57BL/6 kidney ILC2s since they expressed their transcription factor GATA3 and the IL-2, IL-7, and IL-25 receptors at higher levels. Indeed, they also demonstrated greater responsiveness to IL-2 than C57BL/6 kidney ILC2s, as shown by their greater STAT5 phosphorylation levels after culture with IL-2. Thus, this study demonstrates previously unknown properties of kidney ILC2s. It also shows the impact of mouse strain background on ILC2 behavior, which should be considered when conducting research on immune diseases with experimental mouse models.

• Journal of Advanced Marine Engineering and Technology
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• 제37권7호
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• pp.743-751
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• 2013

In this paper, an approach to deal with model uncertainty using norm-optimal iterative learning control (ILC) is mentioned. Model uncertainty generally degrades the convergence and performance of conventional learning algorithms. To deal with model uncertainty, a worst-case norm-optimal ILC is introduced. The problem is then reformulated as a convex minimization problem, which can be solved efficiently to generate the control signal. The paper also investigates the relationship between the proposed approach and conventional norm-optimal ILC; where it is found that the suggested design method is equivalent to conventional norm-optimal ILC with trial-varying parameters. Finally, simulation results of the presented technique are given.

• Journal of Advanced Marine Engineering and Technology
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• 제38권10호
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• pp.1281-1286
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• 2014

This paper presents an iterative learning control (ILC) approach for tracking problems with specified data points that are desired points at certain time instants. To design ILC systems for such problems, unlike traditional ILC approaches, an algorithm which updates not only the control signal but also the reference trajectory at each trial will be developed. The relationship between the reference trajectory and ILC control in tracking problems where there are specified data points through which the system should pass is investigated as the rate of convergence. In traditional ILC, the desired data is stored in a tracking profile file. Due to the huge size of the data file containing the target points, it is important to reduce the computational cost. Finally, simulation results of the presented technique are mentioned and compared to other related works to confirm the effectiveness of proposed scheme.

• 한국어병학회지
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• 제17권2호
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• pp.75-82
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• 2004

인터페론은 바이러스에 감염된 세포에서 생성되어 다른 세포로 하여금 바이러스의 증식을 억제하는 단백질을 합성하게 하는 사이토카인의 일종으로서 바이러스에 대한 방어작용에서 중요한 역할을 담당한다. 본 연구 결과 강력한 인터페론 유도물질로 알려져 있는 poly inosinic : cytidylic acid (poly I:C)를 잉어에 주사한 결과 SVCV에 대한 항바이러스 활성을 확인할 수 있었다. 즉, Poly I:C 주사구에서 대조구에 비해 누적폐사율이 감소하였으며, 또한 두신백혈구를 분리하여, poly I:C를 처리한 결과 interferon-like cytokine (ILC)이 생성되었다. Crude ILC의 항바이러스 활성을 cytopathic effect reduction (CPER) assay로 조사한 결과, 적정 HKLs 농도는 1×$10^6$cells/ml으로 나타났으며, 20~50$\mu{g}$/ml 농도의 poly I:C를 처리했을 때 유의적으로 증가하였다. ILC 생성을 위한 적정온도 및 FBS의 농도는 각각 20$20^\circ{C}$와 5%로 나타났다.

• Journal of Advanced Marine Engineering and Technology
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• 제38권10호
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• pp.1303-1309
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• 2014

In this paper, we present an iterative learning control (ILC) framework for tracking problems with predefined data points that are desired points at certain time instants. To design ILC systems for such problems, a new ILC scheme is proposed to produce output curves that pass close to the desired points. Unlike traditional ILC approaches, an algorithm will be developed in which the control signals are generated by solving an optimal ILC problem with respect to the desired sampling points. In another word, it is a direct approach for the multiple points tracking ILC control problem where we do not need to divide the tracking problem into two steps separately as trajectory planning and ILC controller.The strength of the proposed formulation is the methodology to obtain a control signal through learning law only considering the given data points and dynamic system, instead of following the direction of tracking a prior identified trajectory. The key advantage of the proposed approach is to significantly reduce the computational cost. Finally, simulation results will be introduced to confirm the effectiveness of proposed scheme.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 하계학술대회 논문집 D
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• pp.2390-2392
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• 2003

ILC(Iterative Learning Control: 이하 ILC)는 현재 기계, 전기, 화학 등 많은 분야에 널리 적용되고 있다. ILC는 특히 반복적인 trajectory tracking Control 문제에 아주 효과적인 방법 중의 하나이다. 하지만 ILC는 메모리 기반의 scheme로서 trajectory tracking을 위해서는 많은 메모리를 요구하게 된다. 한편, 자세한 관찰에 의하면 인간의 팔, 다리 등의 관절의 움직임은 아주 정확하지가 않다. 이러한 사실로 미루어 인간이 정화한 모션을 취하는데 드는 비용을 줄이고자 모션 명령을 간단히 한다는 가정을 추론 해 낼 수 있다. 이러한 가정에 기초하여 우리는 ILC 명령을 간단히 하기 위해서 약간의 trajectory tracking의 정확성을 회생하는 메커니즘을 제안한다. 간단해진 ILC 명령은 적은 메모리 공간에 저장될 것이다. 또한, 로봇의 trajectory tracking을 위한 기존의 방법들은 아주 복잡할 뿐만 아니라 하나의 task의 수행만이 가능할 뿐 어떤 일반화의 방법도 제시하지 못하고 있다. 그래서 본 논문에서는 ILC 명령의 scaling에 대한 메커니즘을 제공하여 하나의 trajectory에 대해서 비슷한 모양이지만 다른 크기와 속도를 가지는 trajectory를 구현 할 수 있도록 하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2385-2390
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• 2015

Background: Invasive ductal (IDC) and lobular (ILC) carcinomas are the common histological types of breast carcinoma which are difficult to distinguish when poorly differentiated. Discoidin domain receptor (DDR1) and Drosophila dishevelled protein (DVL1) were recently suggested to differentiate IDC from ILC. Objectives: To assess the expression of DDR1 and DVL1 and their association with histological type, grading and hormonal status of IDC and ILC. Materials and Methods: This cross sectional study was conducted on IDC and ILC breast tumours. Tumours were immunohistochemically stained for (DDR1) and (DVL1) as well as estrogen receptor (ER), progesterone receptor (PR) and C-erbB2 receptor. Demographic data including age and ethnicity were obtained from patient records. Results: A total of 51 cases (30 IDCs and 21 ILCs) were assessed. DDR1 and DVL1 expression was not significantly associated with histological type (p=0.57 and p=0.66 respectively). There was no association between DDR1 and DVL1 expression and tumour grade (p=0.32 and p=1.00 respectively), ER (p=0.62 and 0.50 respectively), PR (p=0.38 and p=0.63 respectively) and C-erbB2 expression (p=0.19 and p=0.33 respectively) in IDC. There was no association between DDR1 and DVL1 expression and tumour grade (p=0.52 and p=0.33 respectively), ER (p=0.06 and p=0.76 respectively), PR (p=0.61 and p=0.43 respectively) and C-erbB2 expression (p=0.58 and p=0.76 respectively) in ILC. Conclusions: This study revealed that DDR1 and DVL1 are present in both IDC and ILC regardless of the tumour differentiation. More studies are needed to assess the potential of these two proteins in distinguishing IDC from ILC in breast tumours.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2006년도 제27회 추계학술대회논문집
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• pp.139-145
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• 2006

액체추진제로켓엔진(LRE)은 로켓의 궤도 및 동특성 제어에 있어서 가장 중용한 부분 중 하나이다. LRE 제어 목적은 주어진 추력 궤도에 맞추어 추력을 조절하는 것과 주연소실과 가스발생기 내의 연소가스의 온도가 일정 범위를 넘어가지 않도록 추진제의 혼합비를 일정하게 유지시키는 것이다. 이런 제어 목적을 가진 LRE는 LRE를 구성하고 있는 구성품 간의 상호간섭에 의하여 다중제어가 쉽게 이루어지지 않는다. 본 연구에서는 LRE에 대한 동특성 모델을 구성하였으며 PID 제어와 PID+Q-ILC 제어로직을 적용한 결과에 대해 해석하였다. 전산모사 결과, PID 제어 보다 PID+Q-ILC 제어 방식을 적용할 경우 오차를 더욱 더 줄일 수 있는 것을 확인하였다.