• KSBB Journal
• /
• 제30권6호
• /
• pp.307-312
• /
• 2015

Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and $k_La$. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.

• BMB Reports
• /
• 제43권8호
• /
• pp.561-566
• /
• 2010

Though protein transduction domains (PTDs) are well known for the delivery of exogenous therapeutic proteins into living cells, the overall low efficiency of transduction is a serious obstacle. We investigated the effect of bog blueberry anthocyanins (BBA) on protein transduction efficiency and found that BBA enhanced the transduction efficiencies of Tat-SOD fusion protein into HeLa cells and mice skin. The enzymatic activities in the cells and skin tissue in the presence of BBA were markedly increased compared to controls. Further, BBA did not demonstrate any cell toxicity at various concentrations. Although the mechanism is not fully understood, we suggest that BBA might alter the conformation of the membrane, which would indicate that BBA can be used as a protein transduction enhancer for the efficient delivery of therapeutic proteins for a variety of disorders.

• 미생물학회지
• /
• 제32권3호
• /
• pp.186-191
• /
• 1994

Staphylococcus aureus로부터 분리한 R-plasmid pSBK203의 복제개시 단백질인 Rep의 작용부위인 ori 및 dsDNA로의 전환을 위해 요구되는 minus origin부위를 밝히고자 시도하였다. Escherichia coli vecotr를 이용하여 pSBK203의 복제관련 부위를 최소한도로 포함하는 재조합 E.coli-Bacillus subtilis shuttle vector를 구성, 분리하고 여기에 포함된 pSBK203부위의 염기 서열을 분석함으로써 ori를 확인하였다. pSBK203의 복제개시 부위 ori는 rep의 구조 유전자 ORF내에서 약 50bp의 크기로 발견되었으며 지금까지 알려진 staphylococcal plasmid들중에서 pT181족 plasmid들의 ori와 높은 상동성을 갖는 것으로 분석되었다. 복제 과정에서 ssDNA로 먼저 만들어진 (+)쇄가 dsDNA로 전환되기 위해 필요한 신호로 작용하는 것으로 알려저 있는 minus origin (M-O)인 긴 palindrome 구조, 즉 pal 부위가 rep 우전자의 상류에서 2개 연이어 존재하는 것이 발견되었다. 이중에서 pOX6, pC194, 및 pE194 등과 같은 다른 staphylococcal plasmid들의 pal 부위와 비교적 높은 상동성을 갖는 paLA 는 plasmid 유지에 별 영향을 미치지 못하는 반면 다른 plasmid에서 유사 서열이 보고되지 않은 palA는 plasmid 유지에 필수적이라는 사실이 밝혀졌다.

• 지역사회간호학회지
• /
• 제14권2호
• /
• pp.253-262
• /
• 2003

Purpose: This study was carried out to identify factors influencing osteoporosis in women at pre- and post-menopausal state. Methods: The subjects of this study were 52 pre-menopausal and 125 post-menopausal women who were assessed of bone density in one general hospital. The data were collected through review of clinical records and telephone interviews using a questionnaire. Results: In the pre-menopausal women, the factors influencing osteoporosis were regular exercise (protective factor) and age (risk factor). Regression analysis showed that the factors attributable to osteoporosis included educational level, weight, age and number of pregnancy, accounted for 41.89% of the total variance. In the post-menopausal women. the factors influencing osteoporosis were age (risk factor), low educational level (risk factor), low economical state (risk factor), high parity (risk factor), and intake of coffee (protective factor). Regression analysis also showed that factors attributable to their osteoporosis included age, educational level, number of delivery, intake of the coffee, regular exercise, number of pregnancy and duration of oral pill intake, accounted for 37.41 % of the total variance. Conclusion: In pre-menopausal women, regular exercise was one of the most powerful determinant of their bone mass. Therefore, it is necessary to participate in a regular exercise program to maintain peak bone mass density prior to the onset of menopause. In post-menopausal women, increased age was the most influencing factor of their bone mass. Therefore, it is essential to establish early diagnosis and management of osteoporosis after menopause.

• 한국강구조학회 논문집
• /
• 제18권3호
• /
• pp.385-393
• /
• 2006

목구조의 효율적인 이용은 건설산업에 있어 자재생산에 따른 에너지의 대량소비 및 폐기물유발을 감소할 수 있어 친환경적인대응방안으로 주목받고 있다. 이러한 친환경적인 목재의 수요를 증대시키기 위해서는 중 대형 목구조를 필요로 하는데 기존의 단일부재 및 접합부로써 중 대형 목구조를 실현하는 데는 한계가 있다. 그러므로 중 대형 목구조의 가능성을 높이기 위해서는 구조용집성재의 사용 및 다른 재료를 병합한 효율적인 접합방법이 필요로 하게 된다. 본 연구는 중 대형 목구조용 2방향 라멘접합부 개발을 목표로 하여 H형강과 구조용집성재를 사용한 플랜지의 두께(None, 6mm, 9mm, 12mm)이며, 접합부의 휨 실험을 통해 강성, 강도, 구조용집성재의 응력분포, H형강 플랜지의 변형도 및 파괴형상을 파악하였다. 실험결과, H 형강의 플랜지 두께가 9mm, 12mmm인 실험체는 높은 강도와 우수한 변형능력을 발휘하였다. 또한 H 형강의 플랜지 두께가 9mm, 12mm인 실험체의 이력거동은 매우 크고 매우 높은 에너지 흡수능력을 가지고 있었다.

• 한국환경복원기술학회지
• /
• 제9권6호
• /
• pp.1-12
• /
• 2006

Remote sensing cannot provide a direct measurement of vegetation index (VI) but it can provide a reasonably good estimate of vegetation index, defined as the ratio of satellite bands. The monitoring of vegetation in nearby urban regions is made difficult by the low spatial resolution and temporal resolution image captures. In this study, enhancing spatial resolution method is adapted as to improve a low spatial resolution. Recent studies have successfully estimated normalized difference vegetation index (NDVI) using improved resolution method such as from the Moderate Resolution Imaging Spectroradiometer (MODIS) onboard EOS Terra satellite. Image enhancing spatial resolution is an important tool in remote sensing, as many Earth observation satellites provide both high-resolution and low-resolution multi-spectral images. Examples of enhancement of a MODIS multi-spectral image and a MODIS NDVI image of Cheongju using a Landsat TM high-resolution multi-spectral image are presented. The results are compared with that of the IHS technique is presented for enhancing spatial resolution of multi-spectral bands using a higher resolution data set. To provide a continuous monitoring capability for NDVI, in situ measurements of NDVI from paddy field was carried out in 2004 for comparison with remotely sensed MODIS data. We compare and discuss NDVI estimates from MODIS sensors and in-situ spectroradiometer data over Ochang plain region. These results indicate that the MODIS NDVI is underestimated by approximately 50%.

• Tuberculosis and Respiratory Diseases
• /
• 제69권5호
• /
• pp.375-380
• /
• 2010

Lymphoid interstitial pneumonia (LIP) is a rare benign lymphoproliferative interstitial lung disease. LIP has been associated with autoimmune disorders, HIV, viral infections, and so on. Once underlying systemic diseases have been excluded, a diagnosis of idiopathic LIP can be made. Although 6 cases of pathologically confirmed LIP have occurred in Korea, thus far none has been associated with primary Sjogren's syndrome. A 44-year-old man was admitted to hospital due to a dry cough and dypsnea on exertion that had been ongoing for 2 months. A chest radiography showed multiple and variable-sized cystic lesions, on both lungs and both interstitial infiltration and consolidation in both lower lung fields. Tests for autoantibody showed positive results of anti-nuclear antibody and anti-Ro/La antibody. The patient underwent a video assisted thoracoscopic surgery biopsy and pathologically confirmed LIP. We report the first known case of LIP-associated with primary Sjogren's syndrome in Korea.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 2010년도 하계학술대회 논문집
• /
• pp.330-330
• /
• 2010

이동통신 시스템의 소형화, 경량화, 다기능화 추세에 따라 이동통신 부품도 단위 부피당 소자의 집적도를 증가시키기 위하여 고집적화 추세로 급진전되고 있다. 이에 따라 세라믹 공정 기술도 고집적 추세로 다층화 되고 있어 수동소자의 내장화에 대한 필요성이 증대되고 있다. 이를 위하여 다양한 유전율 대역의 이종소재간 접합을 시도하지만 de-lamination, 내부 crack 등의 결함들이 발생하므로 열팽창계수 조절이 용이한 동종의 글라스를 사용하는 것이 유용하다. 본 연구에서는 공통의 글라스를 개발한 후 다양한 필러들을 혼합하여 mm파 대역에서 다양한 유전율을 갖는 LTCC 소재를 개발함으로써, 수동소자의 내장화에 따른 이종접합시의 매칭성을 극대화하고자 하였다. 이를 위하여 CaO-$Al_2O_3-SiO_2-B_2O_3$계 공통글라스에 $CaZrO_3$, $1.3MgTiO_3$, $2La_2O_3TiO_2$ 필러를 혼합하여 소결체의 미세구조, 유전특성 및 열기계적 특성을 고찰하였다. 이때 유전율 6에서 20에 이르는 저손실 소재를 개발할 수 있었다.

• Journal of Periodontal and Implant Science
• /
• 제24권3호
• /
• pp.541-560
• /
• 1994

The present study was performed to evaluate the relationship between the serotype or the genotype of Actnobacillus actinomycetemcomitans (A. a.) and the severity of periodontal disease. Total 64 A. a. clinical isolates were sampled from 46 sites of 20 subjects classified into the group I (1 periodontally healthy subject, 2 gingivitis patients, 5 ealry adult periodontitis patients), group II (3 moderatelly adult periodontitis patients) and group III (1 advanced adult periodontitis patient, 8 RPP patients). Southern bolt hybridization (fingerprinting) patterns of the five reference strains, A. a. strain ATCC 29523 (serotype a), ATCC 29522 (Serotype b), ATCC 43719 (serotype c), IDH 781 (serotype d) and IDH 1705 (serotype e), were used as the five basic genotypic patterns (A, B, C, D, E). NT type was designated as one which did dnot represent any of those five basic types. The serotypes were determined by ELISA technique with the serum samples from pre-immunized rabbit. Based on subject-based analysis, it was noted that genotypes A and C, NT, and B, D, E were significantly related to the disease groups I, II, and III, respectively. It was also noted that both the serotypes a and c were significantly related to the disease group I and II, while serotypes were significantly related bm), and serotypes b and nd were frequently found in sites with severe attachment loss (LA>6mm). The results indicated that the significant relationship can be delineated beteen the genotypes and the serotypes of Acinobacillus actinomycetemcomitans and the periodontal disease severity. The results also indicated that genotyping can provide more detailed information on its relationship with the disease severity based on both the patient-based and the site-based analyses.

• 대한기계학회논문집A
• /
• 제24권3호
• /
• pp.743-751
• /
• 2000

Because welding residual stress is formidable result in electric resistance spot welding process, and it detrimentally affect to fatigue crack initiation and growth at nugget edge of spot welded la p joints, it should be considered in fatigue analysis. Thus, accurate prediction of residual stress is very important. In this study, nonlinear finite element analysis on welding residual stress generated in process of the spot welding was conducted, and their results were compared with experimental data measured by X-ray diffraction method. By using their results, the maximum principal stress considered welding residual stress at nugget edge of the spot welded lap joint subjected to tension-shear load was calculated by superposition method. And, the $\Delta$P- $N_f$ relations obtained through fatigue, tests on the IB-type spot welded lap joints was systematically rearranged with the maximum principal stress considered welding residual stress. From the results, it was found th2at fatigue strength of the IB-type spot welded lap joints could be systematically and more reasonably rearranged by the maximum principal stress($\sigma$1max-res considered welding residual stress at nugget edge of the spot welding point.