• The Journal of Internal Korean Medicine
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• v.36 no.1
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• pp.22-39
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• 2015

Objectives : The aim of this study was to develop a standard tool of pattern identification for chronic cough, which will be applied to clinical research. Methods : The items and structures of the instrument were based on reviews of textbooks and published literature. The advisor committee on this study consisted of 11 Korean respiratory internal medicine professors, one clinical fellow, and five clinicians with 10 years of experience. The questionnaire, which includes the signs and symptoms of chronic cough, was studied by the delphi method. Delphi examination was carried out via email, through evaluating the importance of symptoms included in each pattern. Results : We divided the pattern identification of chronic cough into five patterns: Wind-Cold, Phlegm-Turbidity, Liver-Fire, Lung Deficiency, and Kidney Yang Deficiency. By the Delphi method and a score evaluation, 38 items were chosen for pattern identification of chronic cough. Conclusions : Through this study, we created a Korean instrument for the pattern identification tool for chronic cough. We expect to apply this tool to subsequent research as its validity and reliability are further confirmed.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.52 no.2
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• pp.149-161
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• 2016

Fishing characteristics of Korean tuna purse seine fishery in the Pacific Ocean were investigated using logbook data compiled from captain onboard and the statistical data from 1980 to 2014. Changes in fishing ground and correlation between marine environmental factors and fishing patterns were investigated using Oceanographic index. The proportion of unassociated set was higher than that of associated set. The catch proportion of yellowfin was higher in the unassociated set, while that of skipjack and bigeye was higher in the associated set. Due to vessels, fishing gears and Korean captains' high-level of skills in fishing technology optimized for the unassociated set and preference of large fishes, especially large yellowfin tuna, it showed unique fishing characteristics focusing on the unassociated set. As for fishing distributions of Korean tuna purse seine fishery and impacts of oceanographic conditions on the fishery, the main fishing ground was concentrated on the area of $5^{\circ}N{\sim}10^{\circ}S$, $140^{\circ}E{\sim}180^{\circ}$ through the decades. When stronger El-nino occurred, the range of fishing ground tended to expand and main fishing ground moved to the eastern part of western and central Pacific Ocean. During this season, yellowfin tuna had high CPUE and catch proportion of yellowfin tuna in the eastern part also increased. As for the proportion of fishing effort by set type, proportion of log associated set was high during El-nino season while that of FAD associated set was high during La-nina season.

• Journal of Microbiology and Biotechnology
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• v.12 no.5
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• pp.807-812
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• 2002

In order to analyze the cellular proteins which interact with core protein of hepatitis C virus (HCV), a yeast two-hybrid screening technique was employed. A carboxyl terminus truncated core protein, which contained amino acid residues from the 1st to 120th, was used as a bait to screen cellular proteins. The expression library prepared from HeLa cell was screened and 400 positive clones were selected. The 75 clones from the positive clones were sequenced and analyzed by undergoing the Blast search. Interestingly, 7 out of the 75 clones encoded E7 antigen of human papilloma virus (HPV). We studied in detail the Interaction between the truncated version of HCV core and E7 antigen in vitro. The core$_{120}$ protein expressed in chimeric form with G57 was able to bring down the E7 protein of HPV type 18 expressed in bacteria. It is therefore suggested that the core of HCV might affect the interaction between E7 and a normal cellular tumor suppressor, known as Rb protein.

• Korean Journal of Veterinary Research
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• v.40 no.4
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• pp.691-699
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• 2000

We investigated the effects of nitric oxide (NO) donors, S-nitroso-L-cysteine (Cys-NO) and 3-morpholinosydnonimine hydrochloride (SIN-1), on the contractile and electrical activity of the circular muscle of guinea pig gastric antrum by using intracellular microelectrode technique. The gastric antral circular muscle showed spontaneous phasic contraction and slow wave of membrane potential. Cys-NO ($0.001{\sim}10{\mu}M$) and SIN-1 ($0.001{\sim}100{\mu}M$) reduced not only the tonic and phasic contraction but also the amplitude of slow wave in a concentration dependent manner. NO donors were more potent to inhibit phasic contraction than to do slow wave. These inhibitory effects of NO donors were mimicked by the membrane permeable guanosine-3',5'-cyclic monophosphate (cGMP) analogue, 8-bromo-cyclic GMP (8-br-cGMP, $10{\sim}300{\mu}M$). The inhibitory effects of SIN-1 and Cys-NO were antagonized by the guanylate cyclase inhibitor, 1H[ [1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ, $10{\mu}M$). These results suggest that the inhibitory effects of NO donors on the mechanical and electrical activity is mainly mediated by cGMP pathway.

• Atmosphere
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• v.22 no.4
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• pp.465-472
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• 2012

Using various observed data, we examined the evolution of tropical Pacific sea surface temperature (SST) during 2011-2012, with focusing on the development of 2012 El Ni$\tilde{n}$o. It is observed that a La Ni$\tilde{n}$a event during 2011 was followed by a moderate El Ni$\tilde{n}$o during 2012 summer. The 2012 summer El Ni$\tilde{n}$o initiated near the west coast of South America on February 2012, and continued to expand westward till August. Given this evolutionary pattern, the 2012 summer El Ni$\tilde{n}$o can be categorized as 'Eastern Pacific (EP) El Ni$\tilde{n}$o' because Ni$\tilde{n}$o-3 index is greater than Ni$\tilde{n}$o-4 index, and it may be the first well-defined EP El Ni$\tilde{n}$o since 2001. On February 2012, this event was initiated mainly by the local air-sea interaction, and at the same time the ocean heat content was accumulated over the tropical western Pacific due to the easterly wind anomaly over the tropical western Pacific. Then, the accumulated heat content slowly propagates to the tropical eastern Pacific, which attributes to maintain El Ni$\tilde{n}$o state during 2012 summer. After August, the positive SST anomaly over the equatorial eastern Pacific decays possibly due to the exhausted heat content and the weakening of air-sea interaction, but the weak positive SST anomaly over the central Pacific remains till now (2012 November).

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2011.06a
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• pp.232-233
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• 2011

이 연구에서는 최근 25년간(1986-2010년)의 우리나라 기상청 및 일본 기상청 자료를 사용하여 엘니뇨 라니냐 현상과 태풍과의 관계를 분석하였다. 여기서는 엘니뇨감시해역의 해면수온의 기준치와의 차의 5개월 이동평균치가 6개월 이상 계속하여 $+0.5^{\circ}C$ 이상 이 된 경우를 엘니뇨현상, $-0.5^{\circ}C$ 이하가 된 경우를 라니냐현상이라고 정의한다. 그리고 엘니뇨 발생년은 엘니뇨현상이 시작된 해부터 종료된 해까지를, 라니냐 발생년은 라니냐현상이 시작된 해부터 종료된 해까지로 정의한다. 주요 분석 결과는 다음과 같다. 전 기간에 대한 태풍의 연평균 발생 수는 25.4개이다. 이는 60년간(1951-2010년)의 연평균 태풍 발생 수 26.3개보다 약 1개 적은 결과로 최근 지구온난화와 관련하여 태풍의 발생 수가 감소 추세를 보이고 있다는 연구 결과를 뒷받침한다. 엘니뇨 발생년의 연평균 태풍 발생 수는 23.9개이고, 라니냐 발생년의 그것은 24.9개이다. 적도 부근 서부 태평양의 따뜻한 물이 동쪽으로 이동하여 동부 태평양의 해면수온이 평년 이상으로 높아지는 엘니뇨 발생년에 태풍의 발생 수가 감소한다는 사실을 알 수 있다. 태풍의 세기를 나타내는 평균 중심최저기압과 평균 최대풍속은 엘니뇨 발생년에 959.3hPa과 35.8m/s, 라니냐 발생년에 965.5hPa과 33.7m/s 그리고 25년 전 기간에 대하여는 962.3hPa과 35.0m/s이었다. 엘니뇨 발생년의 태풍의 세기가 라니냐 발생년의 태풍의 세기보다 강함을 알 수 있다.

• Proceedings of the Korea Water Resources Association Conference
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• 2010.05a
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• pp.938-942
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• 2010

현재 기후변화가 심화되면서 기상변동성이 커지고 이에 따라 사막화 현상의 심화, 엘니뇨(El Nino), 라니냐(La Nina), 태풍, 집중호우 등의 이상기후 현상이 전 지구상에 걸쳐 광역적으로 나타나고 있는 실정이다. 이러한 기후변화는 앞서 말한 것과 같이 여러 기후인자들을 변화시켜 수자원의 양적변화 등 지속가능한 수자원 개발 관리에 큰 영향을 미치므로 이에 대한 연구가 국내외에서 활발히 진행되고 있다. 대표적으로 여러 가지 2CO2 시나리오에 대한 대기 순환 모형의 적용 결과를 이용하여, 이러한 기후변화가 수문순환에 영향을 미치는 기후인자인 기온, 강수량, 습도 및 풍속, 그리고 물의 수량 및 수질 등에 미치는 영향을 분석하고, 이를 기반으로 기후변화와 관련된 환경 및 수자원의 정책 개발에 대한 연구들이 주로 수행되고 있다. 국내 역시 기후 변화와 관련된 연구들이 수행되고는 있으나, 기후변화와 연계된 유량과 수질 예측에 대한 연구가 절실히 요구되고 있는 실정이다. 따라서 본 연구에서는 IPCC의 배출 시나리오(Special Report on Emissions Scenarios, 이하 SRES) 중 인구증가율이 높고 경제발달과 기술변화가 느리고 환경에 무관심한 극한현상을 나타내는 A2 시나리오와 청정 및 자원 효율적인 기술 등 급격히 발전하고 조사대상 유역특성과 유사한 B1 시나리오를 선정하고, 이에 대한 유역의 기온과 강우량을 GCM을 적용하여 모의하였다. 또한 향후의 기후변화가 유출 수질(BOD, TN, TP)에 미치는 영향을 2020년, 2050년, 2080년에 대하여 평가하기 위하여 GIS 기반의 유역 모형인 SWAT을 대상모형으로 선정하였다. 신뢰성 평가를 위해 현재 상태에서의 모의를 검 보정 하여 실제 A2, B1 기후변화 시나리오에 따른 기온 및 강우량 변화 등에 대한 영향을 평가하여 보았다.

• Preventive Nutrition and Food Science
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• v.3 no.1
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• pp.85-91
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• 1998

To investigate the effects of antioxidative vitamins in combination with various fatty acids on breast cancer cell proliferation, MDA-MB231 human breast cancer cells were cultured for 3 days in the serum-free Iscove's modified Dulbecco's medium (IMDM) supplemented with 1.25mg/ml delipidized bovine serum albumin and 10㎍/ml insulin. Alpha-tocopherol, ascorbic acid or both vitamins were added to the medium at the concentrations of 10 and 50μM in the presence of 3μg/ml of oletic(Oa), linoleic(LA) α-linoleinic(LNA) and docosahexaenoic acid(DHA). Cell growth was reduced significantly by α-tocopherol in a dose-dependent manner, but not affected by ascorbic aicd. The four different fatty acids did not have significant effects on cell growth, although DHA exerted inhibitory effect on the growth after 1 day. However, the each fatty acid was well incorporated into celluar lipid as such or elongated forms. Addition of α-tocopherol remarkably increased its celluar contents and reduced cellular levels of thiobarbituric acid substances (TBARS) that were elevated notably in the presence of DHA in the culture media. But ascorbic acid addition did not change much of either cellular α-tocopherol or TBARS contents. northern blot hybridization showed that tumor supressor gene ρ53 was most highly expressed by the combination of ρ-tocopherol and DHA in 8 hours of cell culture. In conclusion , the growth inhibitory effect of vitamin E suggests that breast cancer cell proliferation is reduced by the mechanism other than cytotoxicity of lipid peroxide and it is related to expressionof tumor supprosser gene p53, that can be increased by both vitamin E and n-3 fatty acid, DHA.

• Analytical Science and Technology
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• v.16 no.3
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• pp.206-211
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• 2003

The DC polarograms of some rare earth elements have been investigated in various pH solution and electrolytes. Samarium ion has two well-defined reduction waves at -1.75 V and -1.95 V in 0.1 M NaCl solution and those are a diffusion controlled in nature. Europium and ytterbium ions give also two step reduction waves at -0.75 V and -1.95 V for europium and -1.45 V and -2.00 V for ytterium in $(C_2H_5)_4NCl$ solution. On the other hand, lantanium and neodium ions show a single reduction wave at -1.75 V. The differences of half wave potentials between europium, ytterium and samarium ions make it possible to determine each ions in the mixed solution sponteniously. In case of europium ion, the stability constants for the complexs of $Eu^{3+}$-EDTA and $Eu^{3+}$-DTPA are evaluated.

• International Journal of Highway Engineering
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• v.11 no.1
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• pp.127-134
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• 2009

This study was performed to establish the process mean which is statistically satisfying the critical limit of deformation strength $(S_D)$, which is found to reflect rutting characteristics of asphalt mixture relatively well. The process means were determined using the critical $S_D$ values (3MPa for local highway and 4MPa for arterial highways), which were obtained from correlation analysis of APA and $S_D$ test data of actual highway projects, and coefficient of variation of 32 combinations of domestic mixtures. The process means of 3.2 MPa for local highway and 4.25 MPa for arterial highways were suggested when using 3 specimens (diameter of 100mm). However, since these values are based on the loading speed of 30mm/min, the higher values, 3.5 MPa for local highway and 4.5 MPa for arterial highways, were suggested, respectively, if the loading speed of 50mm/min is applied using the old Marshall machine, which should give higher test values.