• 동의생리병리학회지
• /
• 제16권2호
• /
• pp.279-288
• /
• 2002

This research investigates the effect of the Crataegus pinnatifida BGE. var. major N.E. BR(CPVM) on Alzheimer's disease. The CPVM extract suppressed the expression of IL-1 β, IL-6, APP, AChE mRNA in PC-12 cells treated with CT105. The CPVM extract suppressed the AChE activity, and the production of APP significantly in PC-12 cells treated with CT105. The CPVM extract group showed a significant inhibitory effect on the memory deficit for the mice with Alzheimer's disease induced by CT105 in the Morris water maze experiment. The CPVM extract suppressed the over-expression of IL-1 β, TNF- α and ROS in the mice with Alzheimer's disease induced by CT105. This study suggests that CPVM may be effective for the prevention and treatment of Alzheimer's disease.

• 동의신경정신과학회지
• /
• 제17권2호
• /
• pp.91-122
• /
• 2006

Objective : This research investigates the effect of the Jangwon-Dan,(JWD) on Alzheimer's disease. Method : The effects of the JWN extract on (1) $IL-1{\beta}$, IL-6, and $TNF-{\alpha}$ mRNA of PC-12 cells treated with LPS; (2) amyloid precursor proteins(APP), acetylcholinesterase(AChE), and glial fibrillary acidic protein(GFAP) mRNA, the AChE activity and the APP production of PC-12 cell treated with CT-105; (3) the behavior; (4) expression of $IL-1{\beta}$, $TNF-{\alpha}$, MDA, $IL-1{\beta}$ mRNA, and $TNF-{\alpha}$ mRNA, (5) the infarction area of the hippocampus, and brain tissue injury in Alzheimer's diseased mice induced with ${\beta}A$ were investigated. Result : 1. The JWN extract suppressed the expression of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ mRNA in THP-1 cells treated with LPS. 2. The JWN extract suppressed the expression of APP, AChE, and GFAP mRNA in PC-12 cells treated with CT-105. 3. The JWN extract suppressed the AChE activity, and the production of APP significantly in PC-12 cells treated with CT-105. 4. For the JWN extract group a significant inhibitory effect on the memory deficit was shown for the mice with Alzheimer's disease induced by ${\beta}A$ in the Morris water maze experiment, which measured stop-through latency, and distance movement-through latency. 5. The JWN extract suppressed the over-expression of $IL-1{\beta}$ protein, $TNF-{\alpha}$ protein, MDA, $IL-1{\beta}$ mRNA, $TNF-{\alpha}$ mRNA, and CD68/GFAP, in the mice with Alzheimer's disease induced by ${\beta}A$. 6. The JWN extract reduced the infarction area of hippocampus, and controlled the injury of brain tissue in the mice with Alzheimer's disease induced by ${\beta}A$. Conclusion : These results suggest that the JWN extract may be effective for the prevention and treatment of Alzheimer's disease. Investigation into the clinical use of the JWN extract for Alzheimer's disease is suggested for future research.

• 동의신경정신과학회지
• /
• 제15권2호
• /
• pp.119-139
• /
• 2004

This research investigates the effect of the Hibiscus syriacus(HSS) on Alzheimer's disease. Specifically, the effects of the HSS extract on (1) $IL-1{\beta}$, IL-6, and $TNF-{\alpha}$ mRNA of PC-12 cells treated with LPS; (2) amyloid precursor proteins(APP), acetylcholinesterase(AChE), and glial fibrillary acidic protein(GFAP) mRNA of PC-12 cells treated with CT-105; (3) the AChE activity and the APP production of PC-12 cell treated with CT-105; (4) the behavior; (4) expression of $IL-1{\beta}$, $TNF-{\alpha}$, $IL-1{\beta}$ mRNA, $TNF-{\alpha}$ mRNA, and reactive oxygen species(ROS); (5) the infarction area of the hippocampus, and brain tissue injury in Alzheimer's diseased mice induced with ${\beta}A$ were investigated. The results were summarized below ; 1. The HSS extract suppressed the expression of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ mRNA in THP-l cells treated with LPS. 2. The HSS extract suppressed the expression of APP, AChE, and GFAP mRNA in PC-12 cells treated with CT-105. 3. The HSS extract suppressed the AChE activity, and the production of APP significantly in PC-12 cells treated with CT-105. 4. For the HSS extract group a significant inhibitory effect on the memory deficit was shown for the mice with Alzheimer's disease induced by ${\beta}A$ in the Morris water maze experiment, which measured stop-through latency, and distance movement-through latency. 5. The HSS extract suppressed the over-expression of $IL-1{\beta}$, $TNF-{\alpha}$, $IL-1{\beta}$ and $TNF-{\alpha}$ mRNA, CD68/GFAP, ROS in the mice with Alzheimer's disease induced by ${\beta}A$. 6. The HSS extract reduced the infarction area of hippocampus, and controlled the injury of brain tissue in the mice with Alzheimer's disease induced by ${\beta}A$. These results suggest that the HSS extract may be effective for the prevention and treatment of Alzheimer's disease. Investigation into the clinical use of the HSS extract for Alzheimer's disease is suggested for future research.

• 한방안이비인후피부과학회지
• /
• 제19권3호통권31호
• /
• pp.55-59
• /
• 2006

항암, 항콜레스테롤, 항산화 및 면역증가 효과와 피부재생 효과 등 여러 가지 생리활성이 밝혀지고 있는 ${\beta}-1,3/1,6-glucan$은 크게 식물성 유래, 효모 및 곰팡이, 버섯유래의 것으로 등으로 분류할 수 있다. 본 연구에서는 이처럼 다양한 효능을 나타내는 버섯균사체로부터 ${\beta}-glucan$를 다량함유하고 보다 쉽게, 대량으로 생산하기 위한 일환으로 연구를 하였다. 이때 고분자 중합체인 ${\beta}-glucan$을 분리하여 핵자기 공명분석기로 분석한 결과 ${\beta}-1,3-$ 및 ${\beta}-1,6-$결합이 혼재되어 있는 ${\beta}-1,3/{\beta}1,6-glucan$의 전형적인 구조임을 확인 할 수 있었다. 또한 ${\beta}-1,3/{\beta}1,6-glucan$의 함량을 gel permeation chromatography으로 분석한 결과 $766{\pm}0.19$ mg/g 함유되었음을 확인 할 수 있었다. 또한 항생재 내성균주인 MRSA의 항균효과를 측정한 결과 MIC가 18mg/mL임을 확인 할 수 있었다. 이 연구결과는 통하여 효과적인 생산방법과 효능을 접목시켜 보다 저렴한 생산비로 대량 생산할 수 있는 방법을 개발할 수 있을 것으로 사료된다.

• BMB Reports
• /
• 제47권6호
• /
• pp.336-341
• /
• 2014

Endothelial cell protein C receptor (EPCR) plays important roles in blood coagulation and inflammation. EPCR activity is markedly changed by ectodomain cleavage and release as the soluble EPCR. EPCR can be shed from the cell surface, which is mediated by tumor necrosis factor-${\alpha}$ converting enzyme (TACE). Oroxylin A (OroA), a major component of Scutellaria baicalensis Georgi, is known to exhibit anti-angiogenic, antiinflammation, and anti-invasive activities. However, little is known about the effects of OroA on EPCR shedding. Data showed that OroA induced potent inhibition of phorbol-12-myristate 13-acetate (PMA), tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and on cecal ligation and puncture (CLP)-induced EPCR shedding through suppression of TACE expression and activity. In addition, treatment with OroA resulted in reduced PMA-stimulated phosphorylation of p38, extracellular regulated kinases (ERK) 1/2, and c-Jun N-terminal kinase (JNK). These results demonstrate the potential of OroA as an anti-sEPCR shedding reagent against PMA and CLP-mediated EPCR shedding.

• Advances in Traditional Medicine
• /
• 제1권2호
• /
• pp.36-44
• /
• 2000

The polysaccharide fractions were isolated and purified from Phellodendron chinese SCHNEID, and antitumor activities using the melanoma B-16-derived metastatic tumors were examined at dosages of 2, 5 and 10 mg/100 g. F-7 and F-8 showed the highest tumor metastatic inhibitory activities (inhibition ratio 60 and 80% in 2 mg/100 g), and in dose of 5 mg/100 g, the inhibitory ratios were 85 and 70%, respectively. Furthermore, 10 mg/100 g of intraperitoneal (i.p.) injection gave 90 and 95% of inhibition. When the effects of polysaccharides on hypersensitivity were examined, the inhibitory activities were not markedly observed in oral administration, indicating that the polysaccharides are directly acting to immune system. Also, the polysaccharides increased the number of circulating blood leukocytes and total peritoneal exudate cells. Although implantation of tumor cells greatly decreased the productivity of antibody (antibody-mediated) and T lymphocyte reactivity (delayed-type) as 6.3 from 9.3 and 5.9 from 7.7, represented by the increase of footpad thickness, respectively. the polysaccharides elevated the reactivity of T lymphocyte in tumor-bearing mice, which were rapidly recovered by discontinuance of sample treatments. Especially, F-2, F-5, F-7 and F-8 remarkably recovered the decreased sensitivity. These results clearly indicated that the i.p. injection is much effective to suppress tumor growth than oral administration.

• 한국식품영양학회지
• /
• 제7권3호
• /
• pp.169-176
• /
• 1994

Screening was performed on edible natural sources to examine superoxide radical scavenging activities by using the method of superoxide dismutase assay. Among 47 kinds of samples, the extract of Capsella bursa-pastoris showed a potent superoxide radical scavenging activity of 11.6 unit / mg solid and was selected for further studies. In order to select optimal extraction solvent system, Cappella bursa-pastoris was extracted with various solvents and the electron donating abilities and inhibitory effects on lipid peroxidation of linoleic acid were measured. Among them, the ethanol extract of Capsella bursa-pastoris possessed the highest level of activities. The ethanol extract of Cappella bursa-pastoris was found to have an inhibitory effect on autoxidation of soybean oil at 5$0^{\circ}C$ from peroxide value, conjugated diene value and refractive index. In the soybean oil containing 0.2% of ethanol extract, induction period was increased 2 times in comparison with control.

• 한국식품과학회지
• /
• 제41권6호
• /
• pp.694-699
• /
• 2009

프로폴리스 에탄올 추출물의 항돌연변이 및 항균효과를 시험하여 국내산과 미국산 프로폴리스의 생리활성을 평가하였다. 항돌연변이 활성은 Ames test로, 항균활성은 여드름 형성에 관여하는 미생물인 P. acnes, S. Epidermidis, S. aureus, P. aeruginosa에 대한 생육 억제효과를 paper disk 법과 agar dilution 법으로 평가하였다. S. Typhimurium TA98 균주에 있어서 $1-200\;{\mu}g$/plate 농도로 70% 에탄올 추출물을 첨가했을 때 Trp-p-1에 의해 유도된 돌연변이에 대해 국내산 0.9-91.3%, 미국산 0.5-104.2%, 2-AA에 의해 유도된 돌연변이에 대해서는 국내산 37.6-94.4%, 미국산 21.0-97.7%의 돌연변이 억제효과를 용량 의존적으로 각각 나타내었다. 항균활성을 paper disk 법으로 평가했을 때 P. acnes, S. Epidermidis, S. aureus, P. aeruginosa에 대해 70% 에탄올 추출물의 $5,000\;{\mu}g$/paper disk 농도에서의 생육 저지환의 크기는 국내산이 각각 5.7, 5.9, 6.8, 5.2 mm, 미국산이 각각 5.7, 6.2, 6.7, 5.9 mm이었다. Agar dilution 법으로 평가한 MIC는 P. acnes, P. aeruginosa S. Epidermidis, S. aureus, P. aeruginosa에 대해 국내산, 미국산 모두 5,000, 1,500, 1,500, $1,500\;{\mu}g/mL$로 나타났다.

• The Korean Journal of Physiology
• /
• 제8권2호
• /
• pp.59-66
• /
• 1974

In recent years much interesting information about the mechanism of the $Na^+-K^+$ activated ATPase has been obtained from investigation of the $K^+-activated$ phosphatase activity which appears to be catalysed by the same enzyme. Also several studies have indicated that a $K^+-activated p-nitrophenylphosphatase activity is intimately related to the ATPase activity. And then the exact relation of p-nitrophenylphosphatase activity to $Na^+-K^+$ ATPase activity is not known. The effects of some ions and drugs on the p-nitrophenylphosphatase activity of the rat brain were investigated and the results were summarized as follows. 1. The p-nitrophenylphosphatase was stimulated markedly by low concentrations of $K^+$, while the activity was activated slightly in the presence of $Na^+$ and oligomycin. 2. Addition of both ATP and $Na^+$ caused a remarkable increase in the activity of the $K^+-dependent$ phosphatase at low concentrations of $K^+$. 3. In the presence of $Na^+$ and low concentrations of $K^+$, oligomycin activated the p-nitrophenylphosphatase. 4. O1igomycin inhibited the stimulation of the enzyme activity caused by $Na^{+}+ATP$. 5. Ouabain inhibited the $K^+-dependent$ p-nitrophenylphosphatase activity more in the presence of ATP and $Na^+$ than in their absence. 6. Quinidine inhibited both $Na^+-K^+$ ATPase and p-nitrophenylphosphatase. These inhibitory effects of the drug were partially antagonized by increasing $K^+$ concentrations. The sensitivity of the $K^+-dependent$ p-nitrophenylphosphatase to quinidine was greater than the that of $Na^+-K^+$ ATPase.

• Natural Product Sciences
• /
• 제20권4호
• /
• pp.274-280
• /
• 2014

Six compounds were isolated from the n-BuOH fraction of the aerial parts of Aruncus dioicus var. kamtschaticus including: sambunigrin (1), prunasin (2), aruncide A (3), aruncide C (4), 1-O-caffeoyl-${\beta}$-D-glucopyranose (5), and caffeic acid (6). Their structures were confirmed by comparing the spectral data with those reported in the literature. The isolated compounds (1 - 6) were then examined for their cytotoxic effects towards MCF-7, HL-60, and HeLa cancer cell lines, as well as their DPPH radical scavenging activity. The results indicated that compound 4 possessed the strongest inhibitory effect toward HeLa cell line with $IC_{50}$ value of $5.38{\pm}0.92{\mu}M$. Compound 3 possessed selective cytotoxic activity on HL-60 cells with $IC_{50}$ value of $6.27{\pm}0.17{\mu}M$, compound 5 was found as the best in inhibiting proliferation with $IC_{50}$ value of $2.25{\pm}0.09{\mu}M$, and the other compounds showed significant inhibition with $IC_{50}$ values ranging from 6.10 to $11.27{\mu}M$. Compound 5 also displayed the strongest cytotoxic effect toward MCF-7 cell line ($IC_{50}$ $4.32{\pm}0.15{\mu}M$). Both 5 and 6 demonstrated strong radical scavenging activity ($IC_{50}$ $6.87{\pm}0.03$ and $4.33{\pm}0.22{\mu}M$, respectively). Compounds 1 and 5 were isolated for the first time from this plant.