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Negative Role of wblA in Response to Oxidative Stress in Streptomyces coelicolor

  • Kim, Jin-Su;Lee, Han-Na;Kim, Pil;Lee, Heung-Shick;Kim, Eung-Soo
    • Journal of Microbiology and Biotechnology
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    • v.22 no.6
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    • pp.736-741
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    • 2012
  • In this study, we analyzed the oxidative stress response of wblA ($\underline{w}$hi$\underline{B}$-$\underline{l}$ike gene $\underline{A}$, SCO3579), which was previously shown to be a global antibiotic down-regulator in Streptomyces coelicolor. Ever since a WblA ortholog named WhcA in Corynebacterium glutamicum was found to play a negative role in the oxidative stress response, S. coelicolor wblA has been proposed to have a similar effect. A wblA-deletion mutant exhibited a less sensitive response to oxidative stress induced by diamide present in solid plate culture. Using real-time RT-PCR analysis, we also compared the transcription levels of oxidative stress-related genes, including sodF, sodF2, sodN, trxB, and trxB2, between S. coelicolor wild type and a wblA-deletion mutant in the presence or absence of oxidative stress. Target genes were expressed higher in the wblA-deletion mutant compared with wild type, both in the absence and presence of oxidative stress. Moreover, expression of these target genes in S. coelicolor wild type was stimulated only in the presence of oxidative stress, suggesting that WblA plays a negative role in the oxidative stress response of S. coelicolor, similar to that of C. glutamicum WhcA, through the transcriptional regulation of oxidative stress-related genes.

A Case Study on the High-quality DCM applied to the Foundation of Breakwater (방파제 기초에 적용된 고품질 DCM공법의 설계 및 시공 사례)

  • Kang, Yeoun-Ike;Shim, Min-Bo;Shim, Sung-Hyun;Kim, Ha-Young;Shim, Jae-Bum;Chun, Youn-Chul;Yoon, Jung-Ik
    • Proceedings of the Korean Geotechical Society Conference
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    • 2009.09a
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    • pp.815-826
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    • 2009
  • The paper presents a case study addressing the design and construction aspects for DCM(Deep Cement Mixing) method employed as the foundation of a caisson type breakwater with heavy weight(10,700 ton/EA) and a high design wave height($H_{1/3}$=8.7m). The DCM was designed for the project(Ulsan New Port North Breakwater Phase 1) by optimizing the pattern of DCM columns with a combination of short and long columns (i.e., block type(upper 3m)+wall type(lower)) and considering overlapped section between columns as a critical section against shear force where the coefficient of effective width of treated column($\alpha$) was estimated with caution. It was shown that the value can be 0.9 under the condition with the overlapped width of 30cm. In addition to that, a field trial test was performed after improving conventional DCM equipment (e.g., mixing blades, cement paste supplying pipes, multi auger motor, etc.) to establish a standardized DCM construction cycle (withdrawal rate of mixing blades) which can provide the prescribed strength. The result of the field strength test for cored DCM specimens shows that the averaged strength is larger than the target strength and the distribution of the strength(with a defect rate of 7%) also satisfies with the quality control normal distribution curve which allows defect rate of 15.9%.

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Study on primary officer military counseling model (초급간부 군 상담모델 개발 연구)

  • Seo, Seon Woo
    • Convergence Security Journal
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    • v.16 no.6_1
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    • pp.75-83
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    • 2016
  • Soldiers management is the core of military combat power to preserve and accident prevention. But in unforeseen times and places did the unthinkable soldier causes an accident, the inexperienced primary officer were upset, and can often be quite nervous. And various accidents prevention is also win the war, the reason for existence in the military for combat readiness as a precondition for perfection. There are primary officers at the forefront of this critical mission. However, in spite of the lack of time to work for combat readiness, orders from higher units of troops management and that's level giving a lot of pressure to primary officer. So I made the primary officer military counseling model ike this. Using Primary officer counseling model is possible to rapid and efficient counseling advice against the target client soldier. The efficient counseling is must take precedence on understanding on client soldier deeply. Depth understanding someone needs a lot of effort and time. However, to Primary officer, it is true that they have a lack of condition that are enough to give the time and effort. Therefore, effective counseling and accident prevention is possible to use counseling model through choice and concentration activities.

Secure Bootstrapping Methods of a Mobile Node on the Mobile IPv6 Network (IPv6기반 이동인터넷 환경에서 이동노드의 안전한 시동에 관한 방법)

  • Nah Jaehoon;Chung Kyoil;Han Chi-Moon
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.42 no.3 s.303
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    • pp.1-8
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    • 2005
  • At IETF (Internet Engineering Task Force), recently RFC3775, RFC3776 documents about the mobile IPv6 were standardized by IETF (Internet Engineering Task Force). Those specifications propose that during the roaming, the mobile node sends securely the binding update to the home agent and the correspondent node after setting the security association between Mobile Node and Home Agent. But there is no secure bootstrapping method between a mobile node and a home agent at the two RFC documents. This paper proposed a method for the secure bootstrapping between a mobile node and a home agent. This makes the authentication, binding update, home agent assignment, security association distribution through the AAA-based secure channel between mobile node and home agent. And the proposed method was analyzed in the view of the procedure, round trip and security strength.

A Study of Market Segmentation for Menu Evaluation of Chinese Tourist (중국관광객의 메뉴개발을 위한 시장세분화에 관한 연구)

  • 강진희;정용주;최안효
    • Culinary science and hospitality research
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    • v.7 no.2
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    • pp.157-179
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    • 2001
  • Today the number or Chinese tourist visiting Korea is expected by 60% more than the number of them in the last year trunks to Chinese government's new policy on opening international trip. Overriding the striking perception of the new Chinese tourism market, the pre-research on this matter is not remarkable so far. It is expected that the theoretical base on the subject should be concrete. the purpose of the article is to provide the out-and-out information including the chinese market, the change of circumstance in China. Futhermore the study is proposed to offer valuable factor evaluating Chinese tourist to choose Korean restaurant and the relational consideration of the menu between estimation and satisfaction at the restaurant during their stay in Korea. In order to achieve the purpose of this paper, it is gore ahead of the literature review including the previous research on the menu of Hotel & Restaurant The major field research is to build a frame of the paper and present the model to release my humble theory. The 1imitation of the study was to make much of the literature basis due to insufficient previous research on the issue, The study would be framed into 6parts 1ike introduction, literature review, analysis of present coalition in China, major study including exploitative research, suggestion and dissuasions & conclusion. After the exploitative research, the study was approached to make findings through the questionnaires designed to find the relationship factor between chinese tourist's evaluation and satisfaction in the Hotel and Restaurant. According to the finding, chinese tourist tend to prefer the group ranging resonable-price and Japanese dishes to other group. They also interested the certain recipe including fish and vegetable. The evaluation menu is directly related to the satisfaction of Chinese tourist and we need a substantial managerial insight on it when the menu is already designed.

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Management Skills for Academic Libraries in the Digital Age (디지털시대 대학도서관의 경영기법에 관한 연구)

  • Lee, Yong-Jae
    • Journal of the Korean Society for Library and Information Science
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    • v.36 no.4
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    • pp.293-309
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    • 2002
  • This study explores what kinds of strategies are appropriate or needed for the academic librarians to upgrade their skills in the digital age. Printed and electronic sources related to this topic are gathered and examined, and communication with some academic librarians in USA is used for this study. The skills suggested in this paper mostly come from business-oriented concepts ike Learning Organization, Knowledge Management, Team Management, Customer-Oriented Services, and Change Management. As a result of this study, it is found that these skills can be used without much difficulties in academic libraries to improve their services. However academic librarians need to keep in mind the core values of academic libraries as an academic forum and non-profit organization when they endeavor to provide better services for customers in the digital age.

A study on the color trend of recent korean passenger car (최근 한국승용차의 색채경향에 관한 연구)

  • 박택용
    • Archives of design research
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    • no.16
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    • pp.53-63
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    • 1996
  • With the high level economic growth and plentiful stabl izing our I iving, our car has become indispensable necessi ties of life which was the symbol of our riches. And yet, on this changes of the times, we welcomed MY CAR epoch with an explosive increase of its demand. However, compared wi th this trend, we feel actually that our level of car development technique is beyond our capaci ty to satisfying our personal fancy I ike advanced nations for buying car in quantity and quality side. On this study, I made car body color as my studing topic which was few concerning to research. I analyzed and investigated[surveyed] the state of envi ronmental facts and recent Korean passenger- car body color trend. After this work, I found out enough to solve the problem on developing car body color. And I suggest the problem solving materials as a reference to meet with the modern's fancy in the development processing of passenger car with systematicaly and effectively.

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Estimation of SOA Weighting Factors for Radar Rainfall Adjustment and Evaluation of Hydrologic Accuracy (레이더강우 보정을 위한 SOA방법의 가중치 산정 및 수문학적 정확성 평가)

  • Noh, Hui-Seong;Kang, Na-Rae;Shin, Hyun-Seok;Kim, Byung-Sik;Kim, Hung-Soo
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.155-155
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    • 2012
  • 최근 집중호우에 따른 자연재해가 크게 증가하여 수문 및 기상분야에서 강우를 관측 및 예측하기 위한 레이더 활용성이 증대되고 있으며, 이에 따라 정부 각 관련부처에서는 레이더의 도입 및 확충을 위한 방안을 계획 제시하고 있다. 특히, 레이더강우 자료는 수문해석분야에서의 GIS 등 디지털정보를 이용하여 유역매개변수를 추정함에 따라 더 정확하고 상세한 수문자료 확보가 가능하게 되어, 격자기반의 분포형 강우-유출모형 등을 이용하여 수문해석을 하는데 있어 그 활용성이 크게 증가하고 있다. 그러나 레이더강우 자료의 정확성은 아직까지 만족할 만한 수준에 이르지 못하고 있기 때문에, 그 불확실성으로 인하여 레이더강우 자료의 활용 및 적용에 한계를 가지고 있는 실정이다. 본 연구에서는 지상강우를 이용한 레이더강우 보정을 위하여 SOA(Statistical Objective Analysis)보정방법을 이용하였으며, 기존 SOA방법에서의 거리에 따른 가중치($_{kd}$)와 함께 지형(고도)에 따른 가중치($W_{ike}$), 바람의 영향에 따른 가중치($W_{ikw}$)를 추가로 산정하여 적용하였으며, 이를 통하여 보정된 강우장을 생성하였다. SOA방법을 통해 생성된 강우장이 어느 정도의 강우정확도를 가지고 강우분포를 재현하는지 검증하기 위하여 2011년 7~9월의 수문학적 분석에 활용 가능한 강우사상과 낙동강유역을 대상으로, 분포형모형인 $Vflo^{TM}$ 모형을 이용하여 산정된 유출량과 관측 유출량과의 비교를 실시하였다. 또한, 유출량에 대한 오차 및 ME(Model Efficiency)를 통해 레이더강우 자료의 유출모형에서의 효율성을 검토하고자 하였다. 본 연구에서 수행된 보정 강우장에 따른 유출량 비교를 통해 레이더 강우의 정확도에 대한 정량적 평가 방법 도출이 가능할 것으로 판단된다.

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Design of MBB System for provide Mobility continuity in Environment IPSec (IPSec 환경에서 연속적인 이동성 제공을 위한 MBB 시스템 설계)

  • Kim, Seon-Young;Jo, In-June
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.12 no.3
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    • pp.478-484
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    • 2008
  • When a mobile node moves, MIPv6 operates an authentication process for the new connection. These kinds of frequent binding update and authentication processes cause much traffic and delay the service. To solve this problem, PMIPv6 provides a network-based mobility protocol in order to lessen the load on a mobile node. However, when it is moved from a domain to a domain or in a domain, there still lies a need fDr a new address, so MIPv6's demerit still exists. In IPsec, too, a new negotiation should be made when it is moved to WAN(Wide Area Network). This causes load to the mobile node. In this paper suggests MBB(Make Before Break) system to eliminate disconnections or delays resulted from the address change or renegotiation for security. When the mobile node receives a CoA address, IPsec negotiation gets operated. Its identity is authenticated by sending the identifier used for the prior negotiation to CN(Correspondent Node) through the BID message suggested. After that, negotiation Bets simplified that disconnections can be eliminated, and in the IPsec negotiation, the load on the mobile node can be lessened as well; moreover, two addresses are used for the communication simultaneously, so the probability of packet loss can be reduced.

Effect of SPZZC, a Composition of Herb Extracts, on Atopic Dermatitis in BALB/c and NC/Nga Mouse (BALB/c 및 NC/Nga 마우스의 아토피성 피부질환모델에서 생약조성물 SPZZC의 치료효능)

  • Lee, Geum-Seon;Pena, Ike Dela;Choi, Ji-Young;Yoon, Seo-Young;Choi, Jong-Hyun;Kang, Tae-Jin;Oh, Se-Koon;Cheong, Jae-Hoon
    • YAKHAK HOEJI
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    • v.52 no.3
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    • pp.232-239
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    • 2008
  • The aim of this study was to evaluate if a composition of herb extracts, PLX-PLS was effective to treat atopic dermatitis (AD) in mice. SPZZC is a composition of herb extracts containing the roots of Scopolia parviflora and Paeonia lactiflora, the herb of Zizania caudiflora, the fruit of Ziziphus jujuba and the leaf of Chinese arborvitae. AD in BALB/c mouse was induced by patching ovoalbumin on the backside, while it in NC/Nga mouse was induced by repeated application of 1-chloro 2,4-dinitrobenzene (DNCB). Mice were topically treated with SPZZC or Domohorn ointment on the backside for 2 weeks (BALB/c) or 1 week (NC/Nga). Scratching behavior, clinical skin severity and the levels of WBC, neutrophil, eosinophil and total serum IgE were measured. After AD induction, scores of scratching behavior and clinical skin severity and the levels of WBC, neutrophil, eosinophil and total serum IgE were increased. Treatment with SPZZC significantly decreased scores of scratching behavior and clinical skin severity in a dose dependent manner in NC/Nga and BALB/c mice. Treatment with SPZZC 2% significantly decreased also the levels of WBC, neutrophil, eosinophil and total serum IgE. Especially, treatment of SPZZC 2% reduced more rapidly score of clinical skin severity than clobetasol cream. These results suggest that the SPZZC may be an alternative substance for the management of AD.