• 제목/요약/키워드: IIS

검색결과 94건 처리시간 0.027초

The New LM-PCR/Shifter Method for the Genotyping of Microorganisms Based on the Use of a Class IIS Restriction Enzyme and Ligation-Mediated PCR

  • Krawczyk, Beata;Leibner-Ciszak, Justyna;Stojowska, Karolina;Kur, Jozef
    • Journal of Microbiology and Biotechnology
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    • 제21권12호
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    • pp.1336-1344
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    • 2011
  • This study details and examines a novel ligation-mediated polymerase chain reaction (LM-PCR) method. Named the LM-PCR/Shifter, it relies on the use of a Class IIS restriction enzyme giving restriction fragments with different 4-base, 5' overhangs, this being the Shifter, and the ligation of appropriate oligonucleotide adapters. A sequence of 4-base, 5' overhangs of the adapter and a 4-base sequence of the 3' end of the primer(s) determine a subset of the genomic restriction fragments, which are amplified by PCR. The method permits the differentiation of bacterial species strains on the basis of the different DNA band patterns obtained after electrophoresis in polyacrylamide gels stained with ethidium bromide and visualized in UV light. The usefulness of the LM-PCR/Shifter method for genotyping is analyzed by a comparison with the restriction endonuclease analysis of chromosomal DNA by the pulsed-field gel electrophoresis (REA-PFGE) and PCR melting profile (PCR MP) methods for isolates of clinical origin. The clustering of the LM-PCR/Shifter fingerprinting data matched those of the REA-PFGE and PCR MP methods. We found that the LM-PCR/Shifter is rapid, and offers good discriminatory power and excellent reproducibility, making it a method that may be effectively applied in epidemiological studies.

Development of Theme Park Management Automation System Using RFID (RFID를 이용한 공원관리 자동화 시스템 개발 연구)

  • Lee, Yang-Weon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • 제9권4호
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    • pp.871-876
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    • 2005
  • Theme park automation system is developed by using the RFID sensor reader, IIS web server, ASP.NET and MSSQL database in this paper. Its operation is that park entrance people checked automatically when he who possessed the ta8 passed the reader acquisition bound and its tag information is sent to the host computer through the RS-232 data acquisition system. Tag information and time data will be stored in the RFID database tables and reused to extract and distribute the entrance people for the purpose of each arrangement of each entertainment devices. First of all, we developed interface program between host and reader and then, programmed the related web site and database connection function using ASP.NET with $C\#$ and MSSQ. We also design and make an production of external Ant. to extend the acquisition range up to $30\~40$ cm using copper PCB plate. We confirmed the performance through the RFID test bed site.

Human Robot Interaction via Intelligent Space

  • Hideki Hashimoto;Lee, Joo-Ho;Kazuyuki Morioka
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 2002년도 ICCAS
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    • pp.49.1-49
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    • 2002
  • $\textbullet$ Intelligent Space 1. Optimal Camera Arrangement 2. People Tracking 3. Physical Robot 4. Robot Control 5. People Following Robot $\textbullet$ Initial stage for making high-level human robot interaction. http://dfs.iis.u-tokyo.ac.jp/∼leejooho/ispace/.

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Conjunctive Query Rewriting in the Context of Data Integration

  • Moon, Kang-Sik;Lee, Jeon-Young
    • Proceedings of the Korea Inteligent Information System Society Conference
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    • 한국지능정보시스템학회 2001년도 The Pacific Aisan Confrence On Intelligent Systems 2001
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    • pp.441-447
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    • 2001
  • The problem of query rewriting using views has interested in the context of data integration where source data is described by the views on global relations. When the query and views are of the form of conjunctive queries, the rewriting is a union of conjunctive queries each of which is contained in the original query and consists of only views. Most previous methods for query rewriting using views are 2-step algorithms. In the first step, they identify the views that are useful in rewriting and in the second step they construct all correct rewritings by combining the views that gained in the first step. The larger the number of selected views in the first step, the larger the number of candidate rewritings in the second step. We want to minimize the number of selected views in the first step by defining stringent conditions for a view to be participated in rewritings. In this paper, first we offer a necessary condition for the existence of a rewriting that includes a view. For the common case that predicate repetitions are not allowed in the bodies of views, we show that our algorithm for testing the condition is done in a polynomial-time. Second, we offer an algorithm to construct contained rewritings using the view instances that are computed in the first step. The exponential containment-mapping test in the second step is not needed in our algorithm.

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Design and Implementation of Web-based Virtual Makeup Simulation System (웹 상에서의 가상 메이크업 시뮬레이션 시스템 설계 및 구현)

  • Shon, Hyung-Do;Lee, Jae-Hyub
    • Journal of KIISE:Computing Practices and Letters
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    • 제8권2호
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    • pp.167-173
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    • 2002
  • The VMSS(Virtual Makeup Simulation System) is a virtual simulation system which can apply makeup, hair style, accessory to user's own face image through internee connection. The simulation result can be stored to server side DB and also can be printed out on client side printer. It is implemented using Java so, clients don't need any other client program to run the VMSS except Web browser and Java Plug-in installed on browser. IIS (Internet Information Server) Web server of Window 2000 and MS-SQL 7 are used to develop the overall system. Commercial JDBC Driver Type 4 is used to connect the applet and DB. It suggests a new direction of Web-based contents service development and can be applied to many other areas.

QoS-Oriented Solutions for Satellite Broadcasting Systems

  • Vargas, Aharon;Gerstacker, Wolfgang H.;Breiling, Marco
    • Journal of Communications and Networks
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    • 제12권6호
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    • pp.558-567
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    • 2010
  • In this paper, we analyze the capability of satellite broadcasting systems to offer different levels of quality of service (QoS). We focus on the European telecommunications standards institute satellite digital radio and digital video broadcasting satellite handheld (DVB-SH) standards, which have recently been proposed for satellite broadcasting communications. We propose a strategy to provide different levels of QoS for the DVB-SH standard on the basis of an extension of the interleaving scheme, referred to as molded interleaver, which supports low latency service requirements for interactive services. An extensive analysis based on laboratory measurements shows the benefits of this solution. We also present a multilevel coding (MLC) scheme with multistage decoding designed for broadcasting communications as an alternative to the existing standards, where services with different levels of QoS are provided. We present a graphical method based on mutual information for the design and evaluation of MLC systems used for broadcasting communications. Extensive simulations for a typical satellite channel show the viability of the proposed MLC scheme. Finally, we introduce multidimensional constellations in the proposed MLC scheme in order to increase the number of different protection levels.

Comparison of Annual Heating and Cooling Loads of Internally and Externally Insulated Apartment Buildings According to the Location of Thermal Mass (내, 외단열 공동주택의 축열체 위치 차이에 따른 동단위 연간 냉난방부하 비교평가)

  • Koo, Bo-Kyoung;Lee, Beung-In;Choi, Doo-Sung;Song, Seung-Yeong
    • Journal of the Korean Solar Energy Society
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    • 제30권1호
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    • pp.42-49
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    • 2010
  • The IIS(Internal Insulation System) is applied in most Korean apartment buildings which are the most common type of residential buildings. Consequently, there are many cases in which the layer of insulation is disconnected by the structural components at the wall-slab and wall-wall joints in the envelope. These joints become thermal bridges where the risk of heat loss increases. It is expected that the EIFS(External Insulation and Finish System) is the solution to this problem. In this study, annual heating and cooling loads of apartment buildings with IIS and EIFS were compared using Design Builder program in order to evaluate the thermal storage effect of EIFS where the concrete thermal mass is located inside of the insulation material. As results, the apartment building with EIFS could reduce annual heating and cooling loads by 2.4% and 4.1%, respectively.

An Implementation of VoiceXML Test Environment Using IIS (IIS를 이용한 VoiceXML 실험 환경 구현)

  • Kwon, Hyung-Joon;Kim, Jung-Hyun;Hong, Kwang-Seok
    • Proceedings of the Korea Institute of Convergence Signal Processing
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    • 한국신호처리시스템학회 2006년도 하계 학술대회 논문집
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    • pp.73-76
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    • 2006
  • 유비쿼터스 컴퓨팅에서 중요한 기술 중 하나로 평가되는 음성인식 및 합성기술은 인간과 컴퓨터의 상호 작용에 있어 가장 편리하고 보편적인 방법이다. 음성인식 및 합성기술을 이용한 인간과 컴퓨터 상호작용 기반의 애플리케이션의 개발을 위해 음성 확장성 생성 언어(VoiceXML)을 이용하면 음성 인식 및 합성에 관한 전문 지식이 없어도 애플리케이션 제작을 쉽게 할 수 있다는 장점이 있어서 음성인식 및 합성기술의 인프라 구축과 저변 확대를 목적으로 일부 국내 업체들은 VoiceXML을 이용한 음성 애플리케이션을 제작하고 실험할 수 있도록 VoiceXML 실험 환경을 제공한다. 본 논문에서는 기존에 공개된 실험 환경을 소개하고, 다양한 실험 환경 제공을 위해 기존에 있던 Linux기반의 실험 환경과는 다른 Windows NT기반의 IIS(Internet Information Service)를 이용한 VoiceXML실험 환경을 제안하고 구현하였다. 그 결과 ASP(Active Server Page)와 ADO(ActiveX Data Object)를 이용한 VoiceXML음성 애플리케이션 실험이 가능한 환경을 구축하였고, 사용자 평가 결과 제안한 방법이 유효하다는 것을 확인하였다.

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Remote Toy Control Utilizing Linux Based ]Embedded System (Linux 기반의 Embedded System을 이용한 모형 자동차 원격제어 구현)

  • 최성백;서정렬;황일훈;정진영;강경하;노근정;최대우
    • Proceedings of the Korea Multimedia Society Conference
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    • 한국멀티미디어학회 2003년도 추계학술발표대회(상)
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    • pp.482-486
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    • 2003
  • 본 논문에서는 PC 또는 mobile phone에서 web server로 접속하여 모형 자동차를 무선 환경에서 원격으로 제어하는 기능을 Linux 기반의 embedded system을 이용하여 구현하였다. 본 system에는 IIS(Internet Information Service) 와 LODEK embedded system 및 ASP(Active Server Pages) 가 사용 되었으며 motor 제어 회로가 구현되었다.

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ASP 웹 디비의 운영과 실행에 관한 사례연구

  • U, Won-Taek
    • Proceedings of the Korea Association of Information Systems Conference
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    • 한국정보시스템학회 2005년도 추계학술대회 발표 논문집
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    • pp.443-454
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    • 2005
  • The purpose of this study is to show the operations and implementations of ASP web database which uses open-source web site packages called bighitonline video shop. Some literature reviews have been done to reveal robust theoretical background for this research And also, some basic experiments as Preparatory Processes have also been made by using some softwares such as Windows XP professional, IIS, MS SQL Server and others. The main purpose of these experiments is to show strong theoretical and practical backgrounds. These experimental results in the micro-level showed to be successful to understand the Interrelations of web components and web-site architectures.

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