• 물과 미래
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• 제32권2호
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• pp.60-61
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• 1999

• 물과 미래
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• 제23권2호
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• pp.140-147
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• 1990

• 물과 미래
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• 제29권2호
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• pp.109-122
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• 1996

• 원예과학기술지
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• 제34권2호
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• pp.314-323
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• 2016

A transient ihpRNA-induced gene silencing system based on Agrobacterium-mediated injection infiltration has been established to evaluate candidate genes involved in proanthocyanidin (PAs) biosynthesis in persimmon (Diospyros kaki Thunb.). We chose DkPDS (phytoene desaturase) as a gene-silencing target to evaluate the newly developed transient gene silencing system. Our qRT-PCR analysis indicated that two ihpRNA constructs (pHG-PDS5' and pHG-PDS3') targeted DkPDS, which also led to significantly reduce expression of DkPDS in 'Mopanshi' persimmon leaves. To further confirm the reliability of the system, we successfully utilized it for DkLAR (leucoanthocyanidin reductase) gene silencing. The expression levels of DkLAR in 'Mopanshi' and 'Eshi 1' leaves were ca. 6-fold and ca. 5-fold lower than those in leaves harboring empty vector (pHG-GFP), respectively. DMACA (4-dimethylaminocinnamaldehyde) staining and the Folin-Ciocalteau assay showed that the accumulation of PAs was markedly inhibited in 'Mopanshi', 'Eshi 1' and 'Youhou' leaves. These results indicate that DkLAR plays an important role in the accumulation of PAs in persimmon. The transient ihpRNA-induced gene silencing method developed in this study is a highly efficient and useful tool for functional analysis of persimmon genes involved in PA biosynthesis.

• 한국통신학회논문지
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• 제30권1C호
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• pp.82-91
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• 2005

본 논문은 iterative hyperplane projection 을 이용한 효율적인 APA(affine projection algorithm)을 소개한다. 다양한 적응 알고리즘들 중 APA는 랭크 부족 문제를 해결하며 고속 수렴의 특성 때문에 다양한 응용분야에 적용되고 있다. SIMO(Single-Input-Multiple-Output) 시스템을 위한 STDE(Space-Time Decision- directed Equalizer) 적용 시 흔히 단일 채널 환경에서 발생하는 "shifting invariance property"를 이용할 수 없으므로 인해 FTF(Fast Transversal Filter)와 같이 저 복잡도를 갖는 고속 적응 알고리즘을 사용할 수 없다. 따라서 APA 기반의 STDE 기능을 수행하는 과정에서 SMI(Sample Matrix Inversion) 처리가 불가피하며, 계산상의 복잡도가 증가하게 된다. 이러한 문제점을 해결하고자 본 논문에서는 APA 기법 고유의 우수한 추적 특성 및 고속 수렴 성질을 유지하면서, 낮은 복잡도를 갖는 IHP(Iterative Hyperplane Projection) 알고리즘 기반의 효율적인 수정 APA 기법을 소개한다. 제안된 IHP 기반 APA 기법의 성능을 확인하기 위하여, 무선 SIMO 채널 환경 하에서 제안된 IHP-APA 알고리즘을 적용한 STED에 대한 비트 에러 오률 (BER) 특성과 계산량 분석을 통해서 우수성을 입증하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 추계학술대회 논문집
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• pp.1215-1220
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• 2004

Cell adhesion to any material surface is considered to be fundamental and important phenomenon in the fields of tissue engineering. Cell adhesion molecules, mechanism, and attachment force have been studied and described a lot. However, the effects of mechanical stimuli on the adhesive forces still have been left much to be investigated. In this study, to investigate the changes in cell adhesive force due to resting time period during the intermittent hydrostatic pressurizing (IHP), cells were cultured under the IHP with various resting times. Then the cell adhesive forces were measured quantitatively utilizing a cell detachment test system and immunofluorescent staining was performed using fluorescent microscopy. In the results, immediately after mechanical stimuli (150 minutes after seeding) and one hour later (210 minutes after seeding), the average adhesive force of experimental group 5 (resting time: 15min) compared with that of control group at same culture time was increased significantly (p<0.05). The results indicated that IHP can contribute in improving cell adhesive force and some of time intervals were required for the expression of cell response.

• The Journal of Korean Physical Therapy
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• 제25권4호
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• pp.217-223
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• 2013

Purpose: This study was to investigate whether the two different head postures, natural and ideal head posture, affect head/shoulder posture and muscle activity. Methods: Thirty healthy subjects with the forward head and round shoulder posture were participated in this study. This study utilized a within-subjects design with subjects being positioned into two sitting positions: natural head posture (NHP) and ideal head posture (IHP). Forward head angle (FHA) and forward shoulder angle (FSA) of each subject were measured for assessing the head/shoulder posture and muscle activities of upper trapezius (UT), lower trapezius (LT), and serratus anterior (SA) during the forward overhead reaching. Results: There were significant increases in both FHA and FSA after taking IHP, which showed greater angles than in taking NHP. In change of muscle activities, there were significant decreases in both LT and SA after taking IHP, which showed lower activities than in taking a NHP, whereas there was no significant change in UT. Conclusion: These findings demonstrate that postural alterations associated with forward head and rounded shoulder postures could alter scapular kinetics and muscle activity during the forward overhead reaching.

• 물과 미래
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• 제40권6호
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• pp.24-34
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• 2007

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 1994년도 수공학연구발표회논문집
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• pp.73-78
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• 1994

• 물과 미래
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• 제49권4호
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• pp.8-13
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• 2016