• 제목/요약/키워드: IHA

검색결과 40건 처리시간 0.047초

Diagnostic value of a dot immunobinding assay for human pulmonary hydatidosis

  • OLUT Ali I.;ERGUVEN Sibel;EMRI Salih;OZUNLU Haluk;AKAY Hadi
    • Parasites, Hosts and Diseases
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    • 제43권1호
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    • pp.15-18
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    • 2005
  • The diagnosis of human hydatidosis is primarily made using radiological and serological methods. Radiological methods are generally of low specificity and serological methods lack sensitivity, especially for pulmonary disease. In this study the capabilities of a new rapid test, the hydatid antigen dot immunobinding assay (HA-DIA), which was developed for the diagnosis of pulmonary hydatidosis, were studied and compared with another immunodiagnostic method, indirect hemagglutination (IHA). The study subjects included 18 patients, 9 women, 9 men; range 7 to 63 years; mean 30 years, with surgically proven pulmonary hydatidosis, a control group comprised of 14 patients; viral respiratory infections (1), cirrhosis (2), connective tissue disease (2), taeniasis (3), and 6 healthy donors. We found that the HA-DIA test had a sensitivity of $67\%$ and specificity of $100\%$, and that the IHA test had a sensitivity of $50\%$ and specificity of $100\%$. We conclude that HA-DIA is a simple, rapid, low cost assay that does not require instrumentation and has a higher sensitivity than IHA for the diagnosis of pulmonary hydatidosis.

돼지의 유구낭충(有鉤囊蟲)에 관(關)한 혈청학적시험(血淸學的試驗) (Serological Diagnosis for Swine Cysticercus Cellulosae Infection in Korea)

  • 전영;이택주
    • 대한수의학회지
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    • 제25권1호
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    • pp.77-89
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    • 1985
  • The agar gel precipitation(AGP), indirect hemagglutination(IHA) and indirect enzyme immunoassay(IEIA) tests were used to detect antibodies in pigs naturally infected with Cysticercus cellulosae in Jeju. The results obtained were summarized as follows: 1. Sera collected from pigs naturally infected with Cysticcrcus cellulosae did not react in AGP test. 2. In the IHA test for swine cysticercosis, the peak titers observed were between 1:20 and 1:160 and non-specific reaction was recognized with a few samples among control sera. 3. In the IEIA test, optical density(OD) values were obtained the best results under the condition of OPD-substrats are reacted at room temperature for 5minutes. The OD values of greater than 0.2 were determined as positive and the high titers in positive sera ranged from 1:40 to 1:1,080. 4. Antibodies to swine cysticercosis were detected by IHA and IEIA tests but the latter was more sensitive and specific than the former. 5. In the preparation of Cystisercus antigens, saline extract which was prepared the precipitate of internal membrane treated ultrasonicator were better results than other antigens for serological tests. 6. Amounts of protein in antigens was not related in direct proportion to results of serological reaction.

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기니픽과 마우스에서 천연형 재조합 사람 Erythropoietin (EPO), DA-3285의 항원성 (Antigenicity of DA-3285, Recombinant Human Erythropoietin, in Guinea Pigs and Mice)

  • 김범준;남석우;박종선;강경구;김원배;한정환;이병무;이향우;홍성렬
    • Biomolecules & Therapeutics
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    • 제4권4호
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    • pp.334-338
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    • 1996
  • Antigenicity of DA-3285, human recombinant erythropoietin which was produced from mammalian cells, was examined in guinea pigs and mice. In active systemic anaphylactic test, mild anaphylactic signs were observed in guinea pigs sensitized subcutaneously with DA-3285 or DA-3285 incorporated with complete Freund's adjuvant(CFA) when challenged with high dose(1000 IU/Kg) of DA-3285. Other groups showed negative responses. In mouse-rat passive cutaneous anaphylaxis test, 20% sera of mice immunized with DA-3285 or DA-3285 mixed with aluminum hydroxide(alum) showed mild positive responses. In the case of indirect haemagglutination reaction(IHA) test, when sheep red blood cells coated with DA-3285 was incubated with mouse serum, all the serum samples were showed negative responses. These results suggest that DA-3285 has a very weak antigenic potential and probably would not induce systemic allergic reactions in clinical uses.

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탄력밴드를 이용한 등척성 엉덩관절 벌림 교각운동이 전방머리자세 환자의 족저압에 미치는 영향 (The Effect Of Isometric Hip Abduction Bridge Exercise Using Elastic Band on Foot Pressure in Patients with Forward Head Posture)

  • 유재호;이상빈
    • 대한정형도수물리치료학회지
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    • 제28권3호
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    • pp.41-49
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    • 2022
  • Background: In patients with forward head posture (FHP), the head is positioned forward, causing increased tension in the muscles and structures of the head, neck, and shoulders. This can result in joint dysfunction that may lead to abnormal afferent information. The purpose of this study was to investigate the effect on foot pressure through the isometric hip abduction (IHA) bridge exercise using elastic bands in patients with FHP. Methods: Twenty patients with FHP were randomly assigned to a study group that applied joint mobilization, soft tissue mobilization, a deep neck flexor strengthening exercise, and the IHA bridge exercise using an elastic band. A control group was also constituted, and members were given joint mobilization, soft tissue mobilization therapies, and the deep neck flexor strengthening exercise. Ten patients were assigned to each group. The static foot pressure and dynamic foot pressure of each patient were measured before and after the intervention, and the interventions for each group were applied twice a week for 4 weeks. Results: Both the study group and the control group showed significant differences in static and dynamic foot pressure before and after the interventions (p<.05). There were no significant differences in foot pressure between the study and control groups. Conclusion: : The results of this study revealed that there were no significant differences between the group doing the IHA bridge exercise using the elastic band and the control group. However, the intervention methods applied to both the groups were effective in improving the body center control of FHP patients.

영년채초지에 있어서 혼파조합에 관한 연구 I. 예취빈도와 질소시비수준이 식생구성에 미치는 영향 (Studies on the Mixture combination in rermanent Pasture I. Effects of nitrogen fertilization and cutting management on the botanical composotion)

  • 육완방
    • 한국초지조사료학회지
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    • 제9권2호
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    • pp.68-76
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    • 1989
  • The effect of characteristic of mixture sowing combination on the development of botanical composition was conducted for 12 years on the meadow experiment. The results of this study were summarized as follows. 1. Festuca pratensis lost the expected position of a dominant species. Its vegetation ratio decreased gradually until the last survey year. 2. Arrhenatherum elatius, known as a typical 2-cutting grasss, developed to a dominant species only in the 3- cutting plot without limitation. 3. Alopecurus pratensis, whose feeding value diminishes rapidly, developed to a dominant species regardless of N-fertilization under the condition of 3-cutting, even though the same small amount of seeding was introduced as usual. 4. Trisetum flavescens which causes calcinose exceeded the 40% of vegetation ratio which was the approved rate for feeding at the high N-level (2OOKgIha). 5. Among the introduced, the species showed the significance in quantity were Lotus corniculafus solely in the 2-cutting plot, and Trifolium repens solely in the 3-cutting plot. In the 3-cutting plot legume yet remained under N-fertilization. 6. The appropriate ratio of vegetation for the subordinate species in the mixture sowing combination was easily attained from the least amount of seeding (Gramineae with max. 3Kg/ha, Leguminosae with max. 2kgIha). 7. Into the mixture sowing combination, infiltrated unintroduced species marked more noticeably in the 2- cutting plot than in the 3-cutting plot.

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IHA를 이용한 댐직하류 하천의 수문변화 특성 분석

  • 장창래;김준태
    • 한국수자원학회:학술대회논문집
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    • 한국수자원학회 2008년도 학술발표회 논문집
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    • pp.1116-1120
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    • 2008
  • 댐은 사회경제적으로 다양한 편익을 제공하고 있지만, 댐을 철거하자는 많은 NGO와 다른 환경단체들이 주장하는 생태비용의 이유로 최근에 재평가를 시행하고 있다. 댐 건설로 인한 유사의 차단과 첨두 홍수량의 감소는 하천의 장갑화와 유사의 하구이송을 감소시켜 댐 직하류 하천에 영향을 미치고 있다. 이러한 직 간접적인 영향들은 하도 내에 물리적 서식처 형상, 생태학적 연관성, 이로 인해 하천지형학에서의 변화를 초래하게 된다. 댐 운영이 하류의 하도 및 생태계에 미치는 영향 정도를 정량화하는데 있어 기존의 단순한 유황 분석을 통한 갈수량, 저수량, 평수량, 풍수량은 댐으로 인한 하류 하천의 영향을 정량적으로 분석하는 데는 한계가 있다. 따라서 본 연구에서는 다양한 수문변화를 정량적으로 계산할 수 있는 IHA(Indicators of Hydrologic Alteration) 모형을 이용하여 합천댐 직하류 하천의 수문변화 특성을 분석하였다. 댐의 유입량과 방류량을 비교한 결과, 대체적으로 홍수기(6월$\sim$9월)에는 댐에 의한 유량 조절효과로 첨두량이 감소하였으며, 그 이외의 월 평균유량은 증가하여 유황이 개선되는 경향을 나타났으며, 수문변화율은 댐의 방류량이 유입량보다 작게 나타났다. 본 연구에서 산정된 수문특성 변화 지표는 댐 유역의 치수, 이수, 환경생태적인 변화를 종합적으로 이해하고 적응하는데 있어, 댐 운영을 최적화하거난 효과적으로 이용될 수 있을 것으로 판단된다.

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KSNP+ 원자로덮개 5.5m 수직 낙하 시 원자로내부구조물 건전성 평가 (Evaluation of Reactor Internals Integrity due to 5.5m Concentric Free Fall of KSNP+ Reactor Vessel Closure Head)

  • 남궁인;정승하;이대희;최택상
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2003년도 추계학술대회
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    • pp.1358-1363
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    • 2003
  • Due to the application of Integrated Head Assembly (IHA) in KSNP+ reactor design, an investigation of reactor internals integrity is carried out to assure that the adoption of IHA does not affect the safety of reactor operation. One of the postulated accident events is the R.V. closure head fall from 5.5m high directly above the reactor vessel that may occur during the refueling operation. The analysis model consists of lumped mass elements of the entire reactor vessel and internals. Because of extreme load, separate elastic-plastic analyses are done for the members that undergo plastic deformation. The analysis verified that the stresses of the reactor internals and the fuel assemblies are within the bound of allowable stress limits and the integrity of the fuel assemblies is maintained.

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A Study on Antigenicity of Recombinant Human Interferon $\beta$ (LB00013) in Mice and Guinea Pigs

  • Park, Jong-Il;Jeong, Tae-Cheon;Cha, Shin-Woo;Shin, Ho-Chul;Han, Sang-Seop
    • Toxicological Research
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    • 제12권1호
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    • pp.81-86
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    • 1996
  • Antigenicity of recombinant human interferon $\beta$(LB00013), a newly developed drug for anti-cancer and anti-viral therapeutic use, was investigated in mice and guinea pigs. The following results were obtained: 1. Mice showed no production of antibodies against LB00013 sensitized with aluminum hydroxide gel (Alum) as an adjuvant, when judged by the heterologous passive cutaneous anaphylaxis (PCA) test in rats. Meanwhile, antibodies against ovalbumin (OVA) sensitized with Alum were clearly detected. 2. In guinea pigs, the sensitization of neither LB00013 only nor LB00013 with complete Freund's adjuvant (CFA) produced positive reactions in the homologous active systemic anaphylaxis (ASA) and the PCA tests. Meanwhile, the sensitization of OVA with CFA produced positive reactions in both PCA and ASA. 3. A LB00013-specific reaction was not observed in an indirect hemagglutination(IHA) assay using sera isolated from LB00013 sensitized mice. The present results suggested that LB00013 may have no antigenic potential in mice and guinea pigs.

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