Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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A Study on Antigenicity of Recombinant Human Interferon $\beta$ (LB00013) in Mice and Guinea Pigs

  • Park, Jong-Il (Toxicology Research Center, Korea Research Institute of Chemical Technology) ;
  • Jeong, Tae-Cheon (Toxicology Research Center, Korea Research Institute of Chemical Technology) ;
  • Cha, Shin-Woo (Toxicology Research Center, Korea Research Institute of Chemical Technology) ;
  • Shin, Ho-Chul (Toxicology Research Center, Korea Research Institute of Chemical Technology) ;
  • Han, Sang-Seop (Toxicology Research Center, Korea Research Institute of Chemical Technology)
  • Published : 1996.06.01
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Abstract

Antigenicity of recombinant human interferon $\beta$(LB00013), a newly developed drug for anti-cancer and anti-viral therapeutic use, was investigated in mice and guinea pigs. The following results were obtained: 1. Mice showed no production of antibodies against LB00013 sensitized with aluminum hydroxide gel (Alum) as an adjuvant, when judged by the heterologous passive cutaneous anaphylaxis (PCA) test in rats. Meanwhile, antibodies against ovalbumin (OVA) sensitized with Alum were clearly detected. 2. In guinea pigs, the sensitization of neither LB00013 only nor LB00013 with complete Freund's adjuvant (CFA) produced positive reactions in the homologous active systemic anaphylaxis (ASA) and the PCA tests. Meanwhile, the sensitization of OVA with CFA produced positive reactions in both PCA and ASA. 3. A LB00013-specific reaction was not observed in an indirect hemagglutination(IHA) assay using sera isolated from LB00013 sensitized mice. The present results suggested that LB00013 may have no antigenic potential in mice and guinea pigs.

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