• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1612-1616
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• 2009

Isoflavonoids are a class of phytoestrogens. Isoflavonone synthase (IFS) is responsible for the conversion of naringenin to genistein. IFS is a cytochrome P450 (CYP), and requires cytochrome P450 reductase (CPR) for its activity. Additionally, the majority of cytochrome P450s harbor a membrane binding domain, making them difficult to express in Escherichia coli. In order to resolve these issues, we constructed an inframe fusion of the IFS from red clover (RCIFS) and CPR from rice (RCPR) after removing the membrane binding domain from RCIFS and RCPR. The resultant fusion gene, RCIFS-RCPR, was expressed in E. coli. The conversion of naringenin into genistein was confirmed using this E. coli transformant. Following the optimization of the medium and cell density for biotransformation, $60\;{\mu}M$ of genistein could be generated from $80\;{\mu}M$ of naringenin. This fusion protein approach may be applicable to the expression of other P450s in E. coli.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.78.1-78.1
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• 2014

An ionization front (IF) surrounding an H II region is a sharp interface through which a cold neutral gas makes transition to a warm ionized phase by absorbing UV photons from central massive stars. We investigate the structure and instability of a plane-parallel D-type IF threaded by magnetic fields parallel to the front. We find that magnetic fields increase the maximum propagation speed of the IFs, while reducing the expansion factor, defined as the density ratio of neutral to ionized phases. IFs become unstable to distortional perturbations due to gas expansion across the fronts, exactly analogous to the Darrieus-Landau instability of ablation fronts in terrestrial flames. The growth rate of the IF instability is proportional linearly to the perturbation wavenumber as well as the upstream flow speed. The IF instability is stabilized by gas compressibility and becomes completely quenched when the front is D-critical. The instability is also stabilized by magnetic pressure when the perturbations propagate in the direction perpendicular to the fields. When the perturbations propagate in the direction parallel to the fields, on the other hand, it is magnetic tension that reduces the growth rate, completely suppressing the instability when ${\beta}$ < 1.5, with ${\beta}$ denoting the square of the ratio of the sound speed to the Alfven speed in the pre-IF region. When the front experiences an acceleration, the IF instability cooperates with the Rayleigh-Taylor instability to make the front more unstable. We discuss potential effects of IF instability on the evolution and dynamics of IFs in the interstellar medium.

• Journal of Welding and Joining
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• v.32 no.5
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• pp.50-57
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• 2014

Friction Stir Welding (FSW) is a complex process with several mutually interdependent parameters. A slight difference from known settings may lead to imperfections in the stirred zone. These inhomogeneities affect on the mechanical properties of the FSWed joints. In order to prevent the failure of the welded joint it is necessary to detect the most critical defects non-destructive. Especially critical defects are wormhole and lack of penetration (LOP), because of the difficulty of detection. Online thermography is used process-accompanying for defect detecting. A thermographic camera with a fixed position relating to the welding tool measures the heating-up and the cool down of the welding process. Lap joints with sound weld seam surfaces are manufactured and monitored. Different methods of evaluation of heat distribution and intensity profiles are introduced. It can be demonstrated, that it is possible to detect wormhole and lack of penetration as well as surface defects by analyzing the welding and the cooling process of friction stir welding by passive online thermography measurement. Effects of these defects on mechanical properties are shown by tensile testing.

• The Journal of the Korea Contents Association
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• v.7 no.12
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• pp.322-332
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• 2007

We consider a deformation method suitable for fractal. In IFS fractal, the position of a point is characterized by its code as well as by its coordinates. Code has a meaning of address for fractal. If we move a point by changing its code, the resulting movement shows fractal behavior. We propose three deformation methods based on code information. For the deformation vector of a point in fractal, 1) we use the vector of a given vector field at the point obtained by code transformation, 2) we use the vector constructed by adding predefined displacement vectors according to the code information of the point. Both methods show a fractal-like character as well as an ordinary continuous deformation character. Also, 3) we can deform fern-fractal more naturally by restricting its deforming region using code form.

• Journal of Korea Game Society
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• v.16 no.1
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• pp.119-128
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• 2016

In this paper, we consider an improved deformation method of IFS(iterated function system) fractal using codes of fractal points. In the existing deformation methods, the intermediate results of position dependent partial deformation propagate randomly due to the randomly selected maps of iteration. Therefore, in many cases, the obtained results become somewhat monotonous feeling shapes. To improve these limitations, we propose a method in which the selection of maps are controlled by codes of fractal points. Applying this method, we can obtain interesting fractal deformation conforming with its fractal features. Also, we propose a simple method, incorporating state variables, that can be applied to deformation of some fractal features other than position coordinates.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.3
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• pp.91-98
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• 2021

One of the concerns in the economic policy circle is the fiscal sustainability. This current research revisit the notion of fiscal sustainability for Malaysia using the Indicator of Fiscal Sustainability (IFS) developed by Croce and Juan-Ramón (2003) where we employ samples of time-series data from 1970 to 2017. The findings reveal that 40 out of 48 years, during which the calculated IFS algorithm is above the threshold of 1, imply Malaysia was fiscally unsustainable. Despite having been fiscally unsustainable, Malaysia's fiscal stance shows improvement as a result of fiscal consolidation and fiscal reforms during the sample period. This is shown by the improved calculated IFS algorithm on average, which the value improved from 1.465 in 1970-1993 to 1.377 in 1998-2004 and to 1.146 in the 2006-2013. From the policy front, this indicator can serve as a precautionary early warning measure in formulating future fiscal path for Malaysia. This can be executed by targeting debt ratio and shifting the allocation of expenditures away from less efficient toward more growth-enhancing ones, which eventually would regain fiscal space to counter any incoming economic shocks in the future. This can enhance the fiscal transparency and assist in formulating a fiscal policy strategy in Malaysia.

• Journal of the Korea Society for Simulation
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• v.28 no.3
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• pp.41-48
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• 2019

In modern-war campaign, precision-guided missiles are dominantly used to minimize the collateral damage. Imaging infrared seekers are widely applied for the precise guidance. Due to the high cost of the infrared detector, the cost for the one-shot weapon's test is a burden for the development. To reduce the test cost, a simulation method including imagery tracking is required, which is so-called integrated-flight simulation(IFS). The synthetic image generation(SIG)-based simulation method is typically used, which however cannot represent various environmental and target conditions. In this paper, a new IFS method is proposed using on-sight measured image to overcome the limitations of the SIG-based IFS(SIIFS). The target image acquired at the launching sight has been used only for checking the performance criteria of the image tracker and has not been tried for IFS since it has low resolution and little information. The study described in this paper, however, shows that the on-sight image-based IFS can predict the pre- and mid-course flight performance quite similarly and is very useful for the flight test analysis.

• The Korean Journal of Zoology
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• v.39 no.3
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• pp.273-281
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• 1996

Previously, we have proposed a two-cell type model for follicular steroidogenesis inamphibians with Rana nigromacu lota. Present experiments were carried out to ascertain whether the model Is applicable to R. dybowskii. The role of theca layer were also reassessed by using granulosa cell-free pure theca layer (P-THEP). Theca/epithelium (THEP) layers, P-THEP layers, and granulosa cell enclosed-oocytes () were obtained from ovarian follicles of R. dybowskii by microdissection. Intact follicles (IFs) and different types of tissues were cultured for 6 hour in amphibian Ringer's m the presence or absence of FPII (0.05 gland/mi) or various steroid precursor (100 ng/ml). The amounts of product steroids converted by the components were measured by RIA. Exogenously added pregnenolone (P5) resulted in a marked increase in progesterone (P$_4$) by GCEOs (2143 pg/follicle) and IFs (2346 pg/follicle) but a smaller increase in P4 by THEP layer (495 pg/follicle). Addition of P$_4$ increased 17 a-hydroxyprogesterone (17 $\alpha$-OHP$_4$) levels by GCEOs (1118 pg/follicle) and IFs (1333 pg/follicle) but less by THEP layer (290 pg/follicle). However, much less amounts of P$_4$ or 17 $\alpha$-OHP$_4$ were producad by P-THEP layers than THEP in the presence of P5. Exogenous 1 7$\alpha$-OIIP$_4$ increased androstenedione (AD) levels by GCEOs (1415 pg/follicle) and IFs (561 pg/follicle) but not by THEP layers. In contrast, addition of AD resulted m a marked increase in testosterone (T) levels by TIIEP (2594 pg/follicle) and IFs (2223 pg/follide) but much less by GCEOs (339 pg/follicle). Exogenous T increased estradiol (E$_2$) levels by GCEOs (551pg/follicle) and IFs (887 pg/follicle), but not by THEP layer (<10 pg/follicle). Without addition of FPH or steroid precursors, very low or nondetectable levels of steroids were produced (< 20 pg/follicle) by all the types of follicular components examined. The data presented here indicate that the two-cell type model based on the study with R. nigromacu Iota is applicable to R. dybowskii and also suggest that the minor pathway, which convert P5 to 17$\alpha$-OHP$_4$, is not present in theca layer.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2007.04a
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• pp.25-28
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• 2007

Electroencephalograhic(EEG)는 심리학의 영역에서 인간 두뇌의 활동을 측정 기록하는데 오래 전부터 사용하였다. 과학의 발달함에 따라 점차적으로 인간의 두뇌에서 감정을 조절하는 기본적인 영역들이 밝혀지고 있다. 그래서 인간의 감정을 조절하는 인간의 두뇌 활동 영역들을 EEG를 이용하여 측정하였다. 본 논문에서는 EEG의 신호들과 몸짓을 이용해서 감정을 인식하였다. 특히, 기존에 생체신호나 몸짓 중 한 가지만을 이용하여 각각 실험해서 감성을 인식하였지만, 본 논문에서는 EEG 신호와 몸짓을 동시에 이용해서 피 실험자의 감성을 인식하는 실험을 하였다. 실험결과 기존의 생체신호나 몸짓 한 가지만을 가지고 실험했을 때의 인식률 보다 더 높은 인식률을 보임을 알 수 있었다. 그리고 생체신호와 몸짓들의 특징 신호들은 강화학습의 개념을 이용한 IFS(Interactive Feature Selection)를 이용하여 특징 선택을 하였다.

• Journal of Electrical Engineering and Technology
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• v.9 no.6
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• pp.2058-2064
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• 2014

In transformer fault diagnosis, dissolved gas analysis (DGA) is been widely employed for a long period and numerous methods have been innovated to interpret its results. Still in some cases it fails to identify the corresponding faults. Due to the limitation of training data and non-linearity, the estimation of key-gas ratio in the transformer oil becomes more complicated. This paper presents Intuitionistic Fuzzy expert System (IFS) to diagnose several faults in a transformer. This revised approach is well suitable to diagnosis the transformer faults and the corresponding action to be taken. The proposed method is applied to an independent data of different power transformers and various case studies of historic trends of transformer units. It has been proved to be a very advantageous tool for transformer diagnosis and upkeep planning. This method has been successfully used to identify the type of fault developing within a transformer even if there is conflict in the results of AI technique applied to DGA data.