• Parasites, Hosts and Diseases
• /
• v.45 no.3
• /
• pp.165-174
• /
• 2007

Toxoplasma gondii GRA10 expressed as a GFP-GRA10 fusion protein in HeLa cells moved to the nucleoli within the nucleus rapidly and entirely. GRA10 was concentrated specifically in the dense fibrillar component of the nucleolus morphologically by the overlap of GFP-GRA10 transfection image with IFA images by monoclonal antibodies against GRA10 (Tg378), B23 (nucleophosmin) and C23 (nucleolin). The nucleolar translocalization of GRA10 was caused by a putative nucleolar localizing sequence (NoLS) of GRA10. Interaction of GRA10 with TATA-binding protein associated factor 1B (TAF1B) in the yeast two-hybrid technique was confirmed by GST pull-down assay and immunoprecipitation assay. GRA10 and TAF1B were also co-localized in the nucleolus after co-transfection. The nucleolar condensation of GRA10 was affected by actinomycin D. Expressed GFP-GRA10 was evenly distributed over the nucleoplasm and the nucleolar locations remained as hollows in the nucleoplasm under a low dose of actinomycin D. Nucleolar localizing and interacting of GRA10 with TAF1B suggested the participation of GRA10 in rRNA synthesis of host cells to favor the parasitism of T. gondii.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.11 no.12
• /
• pp.5745-5758
• /
• 2017

In this paper, we develop a simple but very effective 4 by 4 Multiple-Input Multiple-Output (MIMO) antenna system for mobile phones consisting of different types of antennas to achieve low correlation property at the frequency ranges of 1710 to 2170 MHz, which covers wide LTE service bands, from band 1 to band 4. The proposed antenna system consists of two pair of antennas. Each pair consists of a planar inverted-F antenna (PIFA) and a coupling antenna which has the property of the loop. The use of two different antenna types of IFA and a coupling achieves high isolation. Proposed antenna system occupies relatively small area and positions at the four corners of a printed circuit board. The gap between the two antennas is 4 mm, in order to realize the good isolation performance. To evaluate the performance of our proposed antenna system, we perform various experiments. The proposed antenna shows a wide operating bandwidth greater than 460 MHz with isolation between the feeding ports higher than 17.5-dB. It also shows that the proposed antenna has low Envelop Correlation Coefficient (ECC) values smaller than 0.08 over the all desired frequency tuning ranges.

• Journal of Institute of Control, Robotics and Systems
• /
• v.20 no.4
• /
• pp.468-473
• /
• 2014

This paper describes the in-flight alignment of SDINS (Strapdown Inertial Navigation Systems) using an EKF (Extended Kalman Filter) and a UKF (Unscented Kalam Filter), which allow large initial attitude error uncertainty. Regardless of the inertial sensors, there are nonlinear error dynamics of SDINS in cases of large initial attitude errors. A UKF that is one of the nonlinear filtering approaches for IFA (In-Flight Alignment) are used to estimate the attitude errors. Even though the EKF linearized model makes velocity errors when predicting incorrectly in case of large attitude errors, a UKF can represent correctly the velocity errors variations of attitude errors with nonlinear attitude error components. Simulation results and analyses show that a UKF works well to handle large initial attitude errors of SDINS and the alignment error attitude estimation performance are quite improved.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.65 no.6
• /
• pp.1032-1037
• /
• 2016

In this paper, the method of isolation enhancement for the mobile phone MIMO antenna of LTE class 40(2300~2400MHz) was studied. Design of MIMO antenna was based on the hybrid antenna that operates both a monopole and an IFA(Inverted F Antenna). A structure for the isolation enhancement which controls induced electric field on the ground plane is located between MIMO antenna, and was not connected with the ground but apart 0.3mm. A MIMO antenna that operates on class 3~40(1710~2400MHz) of LTE service bands and a structure for the isolation enhancement at the class 10 band were designed. VSWR measurement of implemented antenna on the FR4 board showed within 3:1 at entire design bands, and isolation between antennas at the class 40 band was less than -30dB. Isolation was enhanced more than 20dB by the studied structure. ECC(Envelope Correlation Coefficient) for MIMO performance was under 0.1, and antenna average gain and efficiency measured in the anechoic chamber were -4.28~-1.40dBi and 37.32~72.36% respectively.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.2
• /
• pp.401-410
• /
• 2004

Soil samples from five different areas in Korea were collected during 2001/02 and examined for presence of the genus Clostridium. Direct immuno-fluorescent assay (IFA) examination showed that Clostridium septicum, Cl. novyi and Cl. chauvoei were detected in the soil of specific areas in Korea. Sixteen species of Clostridium were isolated and cultivated from the soil samples. Cl. peifringens was detected in all sampling locations, while the other species were not. The in vitro activity of 14 antibiotic agents was determined against 421 clostridia isolated from the soil contaminated with animal feces in Korea. Trovafloxacin was effective against all isolates of the genus Clostridium except one isolate of Cl. subterminale, two of Cl. tetani, and three of Cl novyi with $MIC_{50}$ $8- 16\mu$g $ml^{-1}$. Thirteen species of Clostridium were resistant to vancomycin except for Cl. perfringens, Cl. sporogenes, and Cl. subterminale. Imipenem and trovafloxacin showed high antimicrobial activities (>95%) against all strains in the clostridia investigated. Therefore, antibiotic agents such as imipenem and trovafloxacin are the most suitable agents for polymicrobial infection as broad-spectrum monotherapy.

• Korean Journal of Veterinary Research
• /
• v.39 no.4
• /
• pp.760-764
• /
• 1999

A totoal of 219 pigs, 109 necropsy-pigs at the diagnostic laboratory of Cheju National University and 110 slaughter-pigs in Cheju, were evaluated for the prevalence of tissue antigen and serum antibody for spontaneus porcine reproductive and respiratory syndrome(PRRS). Tissues from 219 pigs examined for PRRS viral antigen by immmunohistochemistry included lung(cranio-ventral lobes and dorso-caudal lobes), tonsil, tracheobronchial lymph node, mesenteric lymph node, heart, kidney, liver, spleen, testis, ovary, brain, and spinal cord. Sera from 180 pigs were tested for the presence of antibody to PRRS virus by the indirect fluorescent antibody assay (IFA). In the examination of serum antibody and tissue antigen for PRRS virus, serum antibody titers were considered as positive in 10%(18/180) of animals tested and PRRS viral antigen was detected in tissues of 4%(9/219) of the pigs. PRRS virus tissue antigen was most commonly detected by immunohistochemistry in the cranio-ventral lobe and tonsil. We also confirmed the distribution of tissue antigen and prevalence of serum antibody to PRRS virus in Cheju. The detection of viral antigen by immunohistochemistry in tonsils and cranio-ventral lobes proved to be a very useful method for PRRS diagnosis.

• Pediatric Infection and Vaccine
• /
• v.3 no.2
• /
• pp.139-144
• /
• 1996

Purpose : Coxiella burnetii is a well-known causative agent of granuolmatous inflammation and an inducer of morphological change and transformation of human B lymphocyte in vitro. Coxiella burnetii manifests with several clinical symptoms depending upon the organs that are involved. We therefore undertook to clarify the association of Kawasaki disease and Coxiella burnetii. Subjects and Methods : The patient's sera were tested for antibodies specific for Coxiella burnetii, using indirect fluorescent antibody technique(IFA). We compared Coxiella burnetii infection with 3 groups of patients, group 1 (Kawasaki disease), group 2 (other febrile disease) and group 3 (control group). Results : 1) In children with Kawasaki disease (group 1), 93% of the patient tested positive for Coxiella burnetii. 2) In group 2 children, 20% of the patient tested positive for Coxiella burnetii. 3) In group 3 children, 10% of the patient tested positive for Coxiella burnetii 4) There were significant higher positive rate for Coxiella burnetii in Kawasaki disease than group 2 and group 3 (p<0.05). Conclusions : We concluded that our cases of Kawasaki disease were associated with Coxiella burnetii infection. Further studies will be needed to understand the precise role of Coxiella infection in Kawasaki disease.

• Journal of Veterinary Clinics
• /
• v.37 no.2
• /
• pp.67-74
• /
• 2020

The purpose of this study is to identify clinical features of sound (non-lame) dogs with medial patellar luxation (MPL). Medical records of 72 dogs diagnosed with MPL were retrospectively reviewed. There were no significances in breed, sex, body weight, body condition score (BCS), osteoarthritis (OA) score, inclination of the femoral head angle (IFA), and mechanical medial proximal tibial angle (mMPTA) between sound and lame dogs, respectively. The mean age of sound dogs was significantly higher than that of lame dogs (P < 0.05), especially in MPL grade 3. The frequency of sound dogs with MPL grade 1 and 2 was 2.3 times higher than that of sound dogs with MPL grade 3 and 4 (P < 0.05). The anatomical lateral distal femoral angle (aLDFA) of sound dogs was significantly lower than that of lame dogs (P < 0.05). However, there were no statistical differences in aLDFA between sound and lame dogs in MPL grade 1, 2, and 4, except for MPL grade 3. This study suggests that in case of MPL grade 1 and 2, frequency of sound dogs is significantly high, and also that in MPL grade 3, if mean ± (standard deviation) age of the dogs is 7.4 ± 3.6 years old and the aLDFA is 105.6 ± 4.1 degrees, they are very highly possible to be sound dogs.

• Korean Journal of Veterinary Service
• /
• v.27 no.4
• /
• pp.335-343
• /
• 2004

Although human rabies deaths are rare, the disease remains a public health problem in Korea. Here we report the outbreaks and surveillance of animal rabies in Gangwon-do. Animal rabies infections were identified in 119 animals from 1993 to 2003. The $78\%$ of all rabid animals were domestic species in Gangwon-do. Wild Korean raccoon dog (N. p. koreensis) continued to be the only reported rabid wildlife species. Outbreaks of rabies infections in Korean raccoon dogs are found in broad geographic regions across the northern Gangwon-do. The principal rabies hosts today are probably wild animals in Gangwon-do. The malaise, cerebral dysfunction, anxiety, confusion, agitation and abnormal behavior of the animals were the important symptoms of the disease. The Encephalitis, infiltration with lymphocytes and polymorphonuclear leukocytes and the inclusion bodies (Negri bodies) in neuronal cells were the specific histopathological signs. The results of indirect fluorescent antibody test (IFA) for animal rabies diagnosis were identical and the technique was useful to diagnose the disease. Preexposure vaccination is recommended for persons in high-risk groups, such as laboratory workers, veterinarians and certain animal handlers.

• Nuclear Engineering and Technology
• /
• v.55 no.6
• /
• pp.2088-2095
• /
• 2023

The safety of nuclear installations is largely determined by the tightness of fuel elements cladding. As the Fukushima nuclear accident showed, the main task in case of loss of power supply is to ensure reliable removal of residual heat release from spent fuel pool (SFP) with irradiated fuel assemblies (IFAs). The paper presents the results of calculated-experimental studies and thermal-hydraulic modeling of temperature storage modes of IFAs in SFP. Experimental studies of SFP's temperature regime and calculated evaluation of residual heat removal due to the thermal conductivity of building structures surrounding the SFP were performed. To ensure the safe operation of research reactors, it's necessary to know the IFA's residual heat power (RHP) in the reactor and SFP, which is determined depending on the operating time of fuel assemblies (FAs) and the IFAs calculated holding time. The FAs operating time depends on the reactor energy output. The IFAs calculated holding time is determined by the fuel burnup, U-235 mass in the fuel, and reactor utilization factor. The IFAs fuel burnup was calculated using the MCU-PTR program. Also presented are the RHP's calculation results using some of the empirical dependencies. The concept of a passive heat removal system (PHRS) based on thermosyphon's operating principle was proposed.