• Molecules and Cells
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• v.43 no.11
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• pp.899-908
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• 2020

Intrinsically disordered proteins or regions (IDPs or IDRs) are widespread in the eukaryotic proteome. Although lacking stable three-dimensional structures in the free forms, IDRs perform critical functions in various cellular processes. Accordingly, mutations and altered expression of IDRs are associated with many pathological conditions. Hence, it is of great importance to understand at the molecular level how IDRs interact with their binding partners. In particular, discovering the unique interaction features of IDRs originating from their dynamic nature may reveal uncharted regulatory mechanisms of specific biological processes. Here we discuss the mechanisms of the macromolecular interactions mediated by IDRs and present the relevant cellular processes including transcription, cell cycle progression, signaling, and nucleocytoplasmic transport. Of special interest is the multivalent binding nature of IDRs driving assembly of multicomponent macromolecular complexes. Integrating the previous theoretical and experimental investigations, we suggest that such IDR-driven multiprotein complexes can function as versatile allosteric switches to process diverse cellular signals. Finally, we discuss the future challenges and potential medical applications of the IDR research.

• Earthquakes and Structures
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• v.5 no.1
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• pp.83-109
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• 2013

A simple damper optimization method is proposed to find optimal damper allocation for shear buildings under both target added damping ratio and interstorey drift ratio (IDR). The damping coefficients of added dampers are considered as design variables. The cost, which is defined as the sum of damping coefficient of added dampers, is minimized under a target added damping ratio and the upper and the lower constraint of the design variables. In the first stage of proposed algorithm, Simulated Annealing, Nelder Mead and Differential Evolution numerical algorithms are used to solve the proposed optimization problem. The candidate optimal design obtained in the first stage is tested in terms of the IDRs using linear time history analyses for a design earthquake in the second stage. If all IDRs are below the allowable level, iteration of the algorithm is stopped; otherwise, the iteration continues increasing the target damping ratio. By this way, a structural response IDR is also taken into consideration using a snap-back test. In this study, the effects of the selection of upper limit for added dampers, the storey mass distribution and the storey stiffness distribution are all investigated in terms of damper distributions, cost function, added damping ratio and IDRs for 6-storey shear building models. The results of the proposed method are compared with two existing methods in the literature. Optimal designs are also compared with uniform designs according to both IDRs and added damping ratios. The numerical results show that the proposed damper optimization method is easy to apply and is efficient to find optimal damper distribution for a target damping ratio and allowable IDR value.

• Journal of the Korea Institute of Military Science and Technology
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• v.14 no.4
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• pp.590-597
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• 2011

The IDRS provides detection, classification and bearing/range estimation by performing wavefront curvature analysis on an intercepted active transmission from target. Especially, a estimate of the target bearing/range that significantly affects the optimal operation of own submarine is required. Target bearing/range can be estimated by wavefront curvature ranging which use the difference of time arrival at sensors. But estimation ambiguity occur in bearing/range estimation due to a number of peaks caused by high center frequency and limited bandwidth of the intercepted active transmission and distortion caused by noise. As a result the bearing/range estimation performance is degraded. To estimate target bearing/range correctly, bearing/range estimation method that eliminate estimation ambiguity is required. In this paper, therefore, for wavefront curvature ranging, NLS cost function with curve fitting method is proposed, which provide robust bearing/range estimation performance by eliminating estimation ambiguity. Through simulation the performance of the proposed bearing/range estimation methods are verified.

• Structural Engineering and Mechanics
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• v.10 no.4
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• pp.405-426
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• 2000

Two approximate methods based on mechanism analysis suitable for seismic assessment/design of structural concrete are reviewed. The methods involve use of equal energy concept or equal displacement concept along with appropriate patterns of inelastic deformations to relate structure's maximum lateral displacement to member and plastic deformations. One of these methods (Clough's method), defined here as a ductility-based approach, is examined in detail and a modification for its improvement is suggested. The modification is based on estimation of maximum inelastic displacement using inelastic design response spectra (IDRS) as an alternative to using equal energy concept. The IDRS for demand displacement ductilities are developed for a single degree of freedom model subjected to several accelerograms as functions of response modification factor (R), damping ratios, and strain hardening. The suggested revised methodology involves estimation of R as the ratio of elastic strength demand to code level demand, and determination of design base shear using $R_{design}{\leq}R$ and maximum displacement, determination of plastic displacement using IDRS and subsequent local plastic deformations. The methodology is demonstrated for the case of a 10-story precast wall panel building.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1999.10a
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• pp.214-224
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• 1999

The design response spectrum has been widely used in seismic design to estimate force and deformation demands of structures imposed by Earthquake Ground Motion (EQGM). Inelastic Design Response Spectra (IDRS) to specify design yielding strength in seismic codes are obtained by reducing the ordinates of Linear Elastic Design Response Spectrum (LEDRS) by strength reduction factor (R). Since a building is designed using reduced design spectrum (IDRS) rather than LEDRS in current seismic design procedures it allows structures behave inelastically during design level EQGM. Inelastic Response Spectra (IRS) depend not only on the characteristics of the expected ground motion at a given site, but also on the dynamic properties and nonlinear characteristics of a structure. However, it has not been explicitly investigated the effect of different hysteretic models on IRS. In this study, the effect of hysteretic models on IRS is investigated.

• Journal of Internet Computing and Services
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• v.8 no.2
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• pp.55-65
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• 2007

BcN is a high-quality broadband network for multimedia services integrating telecommunication, broadcasting, and Internet seamlessly at anywhere, anytime, and using any device. BcN is Particularly vulnerable to intrusion because it merges various traditional networks, wired, wireless and data networks. Because of this, one of the most important aspects in BcN is security in terms of reliability. So, in this paper, we suggest the sharing mechanism of security data among various service networks on the BcN. This distributed, hierarchical architecture enables BcN to be robust of attacks and failures, controls data traffic going in and out the backbone core through IP edge routers integrated with IDRS. Our proposed anomaly detection scheme on IDRS for BcN service also improves detection rate compared to the previous conventional approaches.

• Proceedings of the Korean Information Science Society Conference
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• 2008.06a
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• pp.134-137
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• 2008

A novel Intrusion Detection and Response System (IDRS) for IP based Ubiquitous Sensor Networks (IP-USN) is proposed. According to the best of our knowledge this is the first security framework for any kind of IP based sensor devices. The proposed scheme is fast, lightweight in terms of computation and memory, which make it appropriate for resource constrained sensor devices.

• Journal of Radiation Protection and Research
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• v.48 no.2
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• pp.100-106
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• 2023

Background: As breast tissue expanders consist of metallic materials in the needle guard and ferromagnetic injection port, irradiation can produce radioactivation. Materials and Methods: A CPX4 (Mentor Worldwide LLD) breast tissue expander was exposed using the Versa HD (Elekta) linear accelerator. Two photon energies of 6 and 10 MV-flattening filter free (FFF) beams with 5,000 monitor units (MU) were irradiated to identify the types of radiation. Furthermore, 300 MU with 10 MV-FFF beam was exposed to the CPX4 breast tissue expander by varying the machine dose rates (MDRs) 600, 1,200, and 2,200 MU/min. To assess the instantaneous dose rates (IDRs) solely from the CPX4, a tissue expander was placed outside the treatment room after beam irradiation, and a portable radioisotope identification device was used to identify the types of radiation and measure IDR. Results and Discussion: After 5,000 MU delivery to the CPX4 breast tissue expander, the energy spectrum whose peak energy of 511 keV was found with 10 MV-FFF, while there was no resultant one with 6 MV-FFF. The time of each measurement was 1 minute, and the mean IDRs from the 10 MV-FFF were 0.407, 0.231, and 0.180 μSv/hr for the three successive measurements. Following 10 MV-FFF beam irradiation with 300 MU indicated around the background level from the first measurement regardless of MDRs. Conclusion: As each institute room entry time protocol varies according to the working hours and occupational doses, we suggest an addition of 1 minute from the institutes' own room entry time protocol in patients with CPX4 tissue expander and the case of radiotherapy vaults equipped with a maximum energy of 10 MV photon beams.

• Molecules and Cells
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• v.45 no.7
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• pp.444-453
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• 2022

Multivalent macromolecular interactions underlie dynamic regulation of diverse biological processes in ever-changing cellular states. These interactions often involve binding of multiple proteins to a linear lattice including intrinsically disordered proteins and the chromosomal DNA with many repeating recognition motifs. Quantitative understanding of such multivalent interactions on a linear lattice is crucial for exploring their unique regulatory potentials in the cellular processes. In this review, the distinctive molecular features of the linear lattice system are first discussed with a particular focus on the overlapping nature of potential protein binding sites within a lattice. Then, we introduce two general quantitative frameworks, combinatorial and conditional probability models, dealing with the overlap problem and relating the binding parameters to the experimentally measurable properties of the linear lattice-protein interactions. To this end, we present two specific examples where the quantitative models have been applied and further extended to provide biological insights into specific cellular processes. In the first case, the conditional probability model was extended to highlight the significant impact of nonspecific binding of transcription factors to the chromosomal DNA on gene-specific transcriptional activities. The second case presents the recently developed combinatorial models to unravel the complex organization of target protein binding sites within an intrinsically disordered region (IDR) of a nucleoporin. In particular, these models have suggested a unique function of IDRs as a molecular switch coupling distinct cellular processes. The quantitative models reviewed here are envisioned to further advance for dissection and functional studies of more complex systems including phase-separated biomolecular condensates.

• Journal of the Korean Magnetic Resonance Society
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• v.18 no.1
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• pp.30-35
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• 2014

The transcription factor CP2 regulates various biological systems at diverse tissues and cells. However, none of the four CP2 isoforms has been solved in structure yet. In particular, two different regions of the CP2b isoform have been characterized to interact with the PIAS1 in nucleus to regulate the ${\alpha}$-globin gene expression. Among them, in this study, the region encompassing residues 251-309 of CP2b was prepared as a recombinant protein and its solution structure was characterized by NMR spectroscopy. The results indicated that the CP2b(251-309) fold belongs to typical IDRs (intrinsically disordered regions), likely to facilitate promiscuous interactions with various target proteins. Unfortunately, however, its interaction with the N-terminal domain of PIAS1 (residues 1-70), which has been identified as one of the CP2b-binding sites, was not observed in the NMR-based titration experiments. Therefore, it could be postulated that the 251-309 region of CP2b would not contact with the PIAS1(1-70), but alternatively interact with another CP2b-binding region that encompasses residues 400-651 of PIAS1.