• Journal of Biomedical Engineering Research
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• v.42 no.3
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• pp.116-124
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• 2021

It is important to differentiate between the target tissue (or organ) and the rest of the tissue before incision during surgery. And when it is necessary to preserve the differentiated tissues, the blood vessels connected to the tissue must be preserved together. Various non-invasive medical imaging methods have been developed for this purpose. We aimed to develop a medical imaging system that can simultaneously apply fluorescence imaging using indocyanine green (ICG) and laser speckle contrast imaging (LSCI) using laser speckle patterns. We designed to collect images directed to the two cameras on a co-axial optical path and to compensate equal optical path length for two optical designs. The light source used for fluorescence and LSCI the same 785 nm wavelength. This system outputs real-time images and is designed to intuitively distinguish target tissues or blood vessels. This system outputs LSCI images up to 37 fps through parallel processing. Fluorescence for ICG and blood flow in animal models were observed throughout the experiment.

• Korean Journal of Food Science and Technology
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• v.43 no.5
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• pp.618-623
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• 2011

Escherichia coli O157:H7 has been considered as a significant food-borne pathogen since its role in causing hemorrhagic colitis and hemolytic uremic syndrome in humans was recognized. In this study, we developed an immunochromatography (ICG) assay for the detection of E. coli O157:H7. E. coli O157:H7 monoclonal antibody (EC MAb) and colloidal gold were conjugated and its specificity was determined by the ICG treated with EC MAb and antimouse IgG at test and control lines, respectively. The detection limit of the ICG was $1{\times}10^5$ CFU/mL, and no crossreactivity was observed to other E. coli strains and major food-borne pathogens. To determine the minimum enrichment time for the ICG, meats and sprouts were inoculated with $1{\times}10$ CFU/100 ${\mu}L$ of E. coli O157:H7. After enrichment time of 10 and 2 h for meats and sprouts, respectively, up to $1{\times}10$ CFU/100 ${\mu}L$ of E. coli O157:H7 could be detected by ICG.

• Journal of Gastric Cancer
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• v.18 no.2
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• pp.161-171
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• 2018

Purpose: This study assessed the feasibility of near-infrared (NIR) imaging with indocyanine green (ICG) in investigating the completeness of laparoscopic lymph node (LN) dissection for gastric cancer. Materials and Methods: Patients scheduled for laparoscopic gastrectomy for treating gastric cancer were enrolled in the study. After intraoperative submucosal ICG injection (0.05 mg/mL), LN dissection was performed under conventional laparoscopic light. After dissection, the LN stations of interest were examined under the NIR mode to locate any extra ICG-stained (E) tissues, which were excised and sent for pathologic confirmation. This technique was tested in 2 steps: infra-pyloric LN dissection (step 1) and review of all stations after proper radical node dissection (step 2). Results: In step 1, 15 patients who underwent laparoscopic pylorus-preserving gastrectomy (LPPG) and 15 patients who underwent laparoscopic distal gastrectomy (LDG) were examined. Seven and 2 E-tissues were obtained during LPPG and LDG, respectively. From the retrieved E-tissues, 1 and 0 tissue obtained during LPPG and LDG, respectively, was confirmed as LN. In step 2, 20 patients were enrolled (13 D1+ dissection and 7 D2 dissection). Six E-tissues were retrieved from 5 patients, and 1 tissue was confirmed as LN in the pathologic review. Overall, 15 E-tissues were detected and removed, and 2 tissues were confirmed as LNs in the pathologic review. Both nodes were from LN station #6, with 1 case each in the LDG and LPPG groups. Conclusions: NIR imaging may provide additional node detection during laparoscopic LN dissection for gastric cancer, especially in the infra-pyloric area.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2007.11a
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• pp.199-201
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• 2007

This paper presents the installation of measuring system for the transient behaviors of grounding system impedances using portable impulse current generator. The measuring system is controller, with controller, power supply, impulse current generator and data aquisition system. In the evaluation of the impulse response in a ground electrode, the step response is calculated based on the ratio of the voltage and current observed.

• Journal of Breast Cancer
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• v.21 no.4
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• pp.447-452
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• 2018

Purpose: The recent trend in breast cancer treatment is to minimize axillary dissection. However, no pattern of axillary metastasis has been precisely established. The purpose of this study was to evaluate the metastatic lymphatic pattern using near-infrared fluorescence imaging with indocyanine green (ICG) in breast cancer with cytologically proven axillary metastasis. Methods: This was a prospective single-center study. We evaluated 147 patients with breast cancer involving cytologically proven axillary metastasis, and compared physiological and nonphysiological lymphatic metastasis. Results: We performed lymphatic mapping for 64 patients who exhibited level II lymphatic flow on near-infrared fluorescence imaging with ICG, and found that all had axillary metastasis: 51 patients who did not receive neoadjuvant chemotherapy (NAC) and 13 patients post-NAC. Of patients who did not receive NAC, 32 had physiological lymphatic metastasis and 19 had nonphysiological lymphatic metastasis. The risk factors for nonphysiological lymphatic metastasis were age ${\geq}55$ years, high Ki-67 index (>20%), and perinodal extension in both univariate and multivariate analysis (p<0.05). Conclusion: Patients with identified risk factors in cytologically-proven axillary metastasis who did not receive NAC may have nonphysiological lymphatic metastasis.

• Journal of Gastric Cancer
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• v.19 no.2
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• pp.157-164
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• 2019

Purpose: Although standard radical gastrectomy is recommended after noncurative resection of endoscopic submucosal dissection (ESD) for early gastric cancer in most cases, residual tumor and lymph node metastasis have not been identified after surgery. The aim of this study is to evaluate the feasibility of sentinel node navigation surgery after noncurative ESD. Materials and Methods: This trial is an investigator-initiated, multicenter prospective phase II trial. Patients who underwent ESD for clinical stage T1N0M0 gastric cancer with noncurative resections were eligible. Qualified investigators who completed the prior phase III trial (SENORITA 1) are exclusively allowed to participate. In this study, 2 detection methods will be used: 1) intraoperative endoscopic submucosal injection of dual tracer, including radioisotope and indocyanine green (ICG) with sentinel basins detected using gamma-probe; 2) endoscopic injection of ICG, with sentinel basins detected using a fluorescence imaging system. Standard laparoscopic gastrectomy with lymphadenectomy will be performed. Sample size is calculated based on the inferior confidence interval of the detection rate of 95%, and the calculated accrual is 237 patients. The primary endpoint is detection rate, and the secondary endpoints are sensitivity and postoperative complications. Conclusions: This study is expected to clarify the feasibility of laparoscopic sentinel basin dissection after noncurative ESD. If the feasibility is demonstrated, a multicenter phase III trial will be initiated to compare laparoscopic sentinel node navigation surgery versus laparoscopic standard gastrectomy in early gastric cancer after endoscopic resection.

• Archives of Plastic Surgery
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• v.49 no.2
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• pp.258-265
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• 2022

Background Chitosan (CS) is a well-known antimicrobial dressing material. Moreover, widely used amniotic membranes contain growth factors beneficial for wound healing. Herein, we created a novel amnion-conjugated CS-alginate membrane dressing and tested its wound healing potency in a diabetic swine model. Methods The bovine amniotic powder growth factor contents were evaluated by protein assay, and the powder's wound healing effects were assessed in vitro by HaCaT cell scratch closure. In vivo, two minipigs developed streptozotocin-induced diabetes. Serial serum glucose measurements and intravenous glucose tolerance tests were performed to confirm their diabetic status. Twelve square-shaped wounds created on each pig's back were randomly divided into control (n = 4), CS (n = 4), and amnion-CS (AC; n = 4) groups and treated accordingly with different dressings. Wound healing in each group was assessed by measuring wound contraction over time, capturing wound perfusion with indocyanine green (ICG) angiography, and histologically analyzing inflammatory markers. Results Amniotic powder elution promoted HaCaT cell migration in the scratch wound model, suggesting its beneficial in vitro wound healing effects. In vivo, the CS and AC groups showed earlier wound contraction initiation and reepithelialization and earlier wound perfusion improvement by ICG angiography than the control group. Additionally, the wound size of the AC group at week 3 was significantly smaller than those in the control group. There was no significant difference in the numbers of acute and chronic inflammatory cells between the groups. Conclusion The amnion-conjugated CS-alginate membrane, as well as CS dressing alone, could be a favorable dressing option for diabetic wounds.

• Journal of KIISE:Software and Applications
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• v.27 no.10
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• pp.1054-1061
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• 2000

영상 정합은 물리적으로 유사한 영상 내의 영역들을 기하학적으로 일치시키는 처리이며 지형 정보, 영상검색, 원격탐사, 의료영상 등의 많은 영상처리 응용에서 사용된다. 영상 정합에 관한 연구는 주로 회전, 크기, 위치 등의 인자 추출에 소요되는 시간과 정확성에 중점을 두어 왔다. 본 연구에서는 영상의 특징 점들에 대한 일차 고유벡터의 방향 분포를 히스토그램으로 표현하고 이를 비교 분석함으로써 정합하는 방법을 제안한다. 일차 고유벡터를 이용함으로써 특징 묘사의 단순성을 제공하고. 히스토그램을 이용하여 정합 인자를 미리 추정함으로써 정합 인자 추출 시 목적함수의 연산에 소요되는 비용을 현저하게 줄였다. 본 연구의 결과를 평가하기 위해 제안한 방식을 일반 영상과 ICG(IndoCyanine Green)망막 영상에 적용한 결과를 보여주고 목적함수의 연산횟수와 시간 복잡도를 기존의 방법들과 비교하였다.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1466-1467
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• 2007

A new parameter W/R for the class I test have been introduced in the IEC 61643-1 published in 2005. One of the possible test impulses which meets the parameters for class I test is the 10/350 [${\mu}s$] waveshape proposed in IEC61312-1. 10/350 [${\mu}s$] waveshape meets parameters for class I test in the IEC 61643-1. The impulse generator for making the 10/350 [${\mu}s$] waveshape is consist of IVG, ICG, crowbar coil and crowbar switch. The electric change Q and the specific energy W/R were measured and calculated using the exclusive use measuring software according to the formula of IEC 611643-1. We verified the correspondence between measured results using impulse generator and recommended parameters by IEC 61643-1.

• Proceedings of the KIEE Conference
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• summer
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• pp.372-374
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• 2004

22.9kV 가공배전선은 여러 요소들로 구성되기 때문에 뇌격에 대한 반응도 단순하지 않다. 현재의 절연협조는 대표적인 선로모델에 패한 선로상수, 접지저항 등의 대표 값을 사용하여 EMTP로 계산한 뇌충격과전압 값을 근거로 하고 있다. 절연협조 수준에 따라 경제성이 결정되고 계산에 의한 모델해석결과는 실제의 현상과 많은 차이를 나타낼 수 있음에도 불구하고 현재의 기준은 계산과정에서 사용되는 모델과 특성 값의 유효성을 확인하는 수준에 머물고 있다. 이 연구에서는 ICG로 충격전류를 인가하여 여러 가지 경우에 애자 양단에 인가되는 과전압을 실측하였다. 실측 결과 접지특성은 애자에 인가되는 과전압에 많은 영향을 미치지 않는다는 것을 알 수 있었다.