• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1155-1164
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• 2019

Lichens contain diverse bioactive secondary metabolites with various chemical and biological properties, which have been widely studied. However, details of the inhibitory mechanisms of their secondary metabolites against influenza A virus (IAV) have not been documented. Here, we investigated the antiviral effect of lichen extracts, obtained from South Korea, against IAV in MDCK cells. Of the lichens tested, Nipponoparmelia laevior (LC24) exhibited the most potent inhibitory effect against IAV infection. LC24 extract significantly increased cell viability, and reduced apoptosis in IAV-infected cells. The LC24 extract also markedly reduced (~ 3.2 log-fold) IAV mRNA expression after 48 h of infection. To understand the antiviral mechanism of LC24 against IAV, proteomic (UPLC-$HDMS^E$) analysis was performed to compare proteome modulation in IAV-infected (V) vs. mock (M) and LC24+IAV (LCV) vs. V cells. Based on Ingenuity Pathway Analysis (IPA), LC24 inhibited IAV infection by modulating several antiviral-related genes and proteins (HSPA4, HSPA5, HSPA8, ANXA1, ANXA2, $HIF-1{\alpha}$, AKT1, MX1, HNRNPH1, HNRNPDL, PDIA3, and VCP) via different signaling pathways, including $HIF-1{\alpha}$ signaling, unfolded protein response, and interferon signaling. These molecules were identified as the specific biomarkers for controlling IAV in vitro and further confirmation of their potential against IAV in vivo is required. Our findings provide a platform for further studies on the application of lichen extracts against IAV.

• Genomics & Informatics
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• v.20 no.4
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• pp.46.1-46.7
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• 2022

Influenza A virus (IAV) is the most widespread pathogen causing human respiratory infections. Although polymerase chain reaction (PCR)-based methods are currently the most commonly used tools for IAV detection, PCR is not ideal for point-of-care testing. In this study, we aimed to develop a more rapid and sensitive method than PCR-based tools to detect IAV using loop-mediated isothermal amplification (LAMP) technology. We designed reverse-transcriptional (RT)-LAMP primers targeting the hemagglutinin gene. RNAs from reference H1N1 and H3N2 showed specific RT-LAMP signals with the designed primers. We optimized the reaction conditions and developed universal reaction conditions for both LAMP assays. Under these conditions, the detection limit was 50 copies for both RT-LAMP assays. There was no non-specific signal to 19 non-IAV respiratory viruses, such as influenza B virus, coronaviruses, and respiratory syncytial viruses. Regarding the reaction time, a positive signal was detected within 25 min after starting the reaction. In conclusion, our RT-LAMP assay has high sensitivity and specificity for the detection of the H1 and H3 subtypes, making it suitable for point-of-care IAV testing.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.15 no.2
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• pp.116-125
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• 2003

The new HVAC system to lower the conveyance energy and building height using IAV (Increasing Air Volume) technique is developed. IAV units which are equipped in each zone carry out air-conditioning and supply fresh air by induction of outdoor air in main duct. The design program which decides size of OAHU and IAV unit according to air conditioning load and fresh air demand of each zone is presented. The control system is developed to operate efficiently HVAC system and IAV unit, so that individual zone operation and well-deal with partial load and IAQ problem are possible. The new system is investigated in model building and makes more profit in conveyance energy, size of air conditioning facilities room and building height than VAV system. But in construction cost it is worse by about 15 per-centage.

• International Journal of Air-Conditioning and Refrigeration
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• v.13 no.1
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• pp.31-43
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• 2005

The new HVAC system is developed to lower the conveyance energy and building height using IAV(Increasing Air Volume) technique. IAV units which are equipped in each zone carry out air-conditioning and supply fresh air by induction of outdoor air in main duct. The design program which decides size of OAHU and IAV unit according to air conditioning load and fresh air demand of each zone is presented. The control system is developed to operate efficiently HVAC system and IAV unit, so that individual zone operation and well-deal with partial load and IAQ problem are possible. The new system is investigated in model building and makes more profit in conveyance energy. size of air conditioning facilities room and building height than VAV system. But in construction cost it is worse by about 15 percentage.

• Journal of the Korea Institute of Military Science and Technology
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• v.20 no.1
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• pp.119-128
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• 2017

G-induced Loss of Consciousness(G-LOC) can be predicted by measuring Electromyogram(EMG) signals. Existing studies have mainly focused on specific body parts and lacked of consideration with quantitative EMG indices. The purpose of this study is to analyze the indices of EMG signals by human body parts for monitoring G-LOC condition. The data of seven EMG features such as Root Mean Square(RMS), Integrated Absolute Value(IAV), and Mean Absolute Value(MAV) for reflecting muscle contraction and Slope Sign Changes(SSC), Waveform Length (WL), Zero Crossing(ZC), and Median Frequency(MF) for representing muscle contraction and fatigue was retrieved from high G-training on a human centrifuge simulator. A total of 19 trainees out of 47 trainees of the Korean Air Force fell into G-LOC condition during the training in attaching EMG sensor to three body parts(neck, abdomen, calf). IAV, MAV, WL, and ZC under condition after G-LOC were decreased by 17 %, 17 %, 18 %, and 4 % comparing to those under condition before G-LOC respectively. Also, RMS, IAV, MAV, and WL in neck part under condition after G-LOC were higher than those under condition before G-LOC; while, those in abdomen and calf part lower. This study suggest that measurement of IAV and WL by attaching EMG sensor to calf part may be optimal for predicting G-LOC.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.63 no.5
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• pp.696-702
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• 2014

Recently, researches on analyzing relationship between the state of emotion and musical stimuli using EEG are increasing. A selection of feature vectors is very important for the performance of EEG pattern classifiers. This paper proposes a comparison of EEG feature vectors for emotion classification according to music listening. For this, we extract some feature vectors like DAMV, IAV, LPC, LPCC from EEG signals in each class related to music listening and compare a separability of the extracted feature vectors using Bhattacharyya distance. So more effective feature vectors are recommended for emotion classification according to music listening.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.33 no.10
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• pp.381-386
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• 1984

This paper deals with the microcomputer realization of EMG pattern recognition system which provides identification of motion commands from the EMG signals for the on-line control of a prosthetic arm. A probabilistic model of pattern is formulated in the feature space of integral absolute value(IAV) to describe the relation between a motion command and the location of corresponding pattern. This model enables the derivation of sample density function of a command in the feature space of IAV. Classification is caried out through the multiclass sequential decision process, where the decision rule and the stopping rule of the process are designed by using the simple mathematical formulas defined as the likelihood probability and the decision measure, respectively. Some floating point algorithms such as addition, multiplication, division, square root and exponential function are developed for calculating the probability density functions and the decision measure. Only six primitive motions and one no motion are incorporated in this paper.

• Korean Journal of Veterinary Service
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• v.45 no.1
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• pp.31-38
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• 2022

Swine influenza (SI) is an important respiratory disease in pigs and epidemic worldwide, which is caused by influenza A virus (IAV) belonging to the family of Orthomyxoviridae. As seen again in the 2009 swine-origin influenza A H1N1 pandemic, pigs are known to be susceptible to swine, avian, and human IAVs, and can serve as a 'mixing vessel' for the generation of novel IAV variants. To this end, the emergence of swine influenza viruses must be kept under close surveillance. Herein, we report the isolation and phylogenetic study of a swine IAV, A/swine/Korea/21810/2021 (sw21810, H3N2 subtype). BLASTN sequence analysis of 8 gene segments of the isolated virus revealed a high degree of nucleotide similarity (94.76 to 100%) to porcine strains circulating in Korea and the United States. Out of 8 genome segments, the HA gene was closely related to that of isolates from cluster I. Additionally, the NA gene of the isolate belonged to a Korean Swine H1N1 origin, and the PB2, PB1, NP and NS genes of the isolate were grouped into that of the Triple reassortant swine H3N2 origin virus. The PA and M genes of the isolate belonged to 2009 Pandemic H1N1 lineage. Human infection with mutants was most common through contact with infected pigs. Our results suggest the need for periodic close monitoring of this novel swine H3N2 influenza virus from a public health perspective.

• Atmosphere
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• v.29 no.2
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• pp.165-181
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• 2019

In this study, the regional climate model, RegCM4.0 (25 km), with the HadGEM2-AO data as boundary conditions, was used to simulate the mean climate changes in the mid and late 21st century for CORDEX Phase 2 East Asian region. 122 years (1979~2100) of simulation were performed, and RCP 4.5 and RCP 8.5 were used for the simulation of future climate. In the mid-21st century, the temperature is expected to increase by about 0.5 to $3.0^{\circ}C$ in all regions of East Asia, regardless of season and scenario. The increase in temperature is greater in summer and winter, especially in the northern part of simulation domain. Interannual variability (IAV) is expected to decrease by 25% in summer for RCP 8.5, while it is expected to increase by more than 30% in autumn for both scenarios. Regardless of the scenario, the precipitation in South Korea is expected to increase in late June but decrease in mid-July, with an increase in precipitation greater than $100mm\;day^{-1}$. In RCP 4.5 of the late 21st century, relatively uniform temperature increase ($1.0{\sim}2.5^{\circ}C$) is expected throughout the continent, while RCP 8.5 shows a very diverse increase ($3.0{\sim}6.0^{\circ}C$) depending on season and geographical location. In addition, the IAV of temperature is expected to decrease by more than 35% in both scenarios in the summer. In most of the Northwest Pacific region, precipitation is expected to decrease in all seasons except for the summer, but in South Korea, it is projected to increase by about 10% in all seasons except autumn.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1998.06a
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• pp.143-147
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• 1998

It sis wisely stated that the most valuable knowledge that a person can acquire is the knowledge of how to learn. The human's learning is characterized by the ability to extract relationships between the different characters of a given situation . The ellipse is a first approach of comparison. We assimilate each character to a half axis of the ellipse and the result is a geometrical figure that varies according to values of the two characters. Thus, we take into account the two characters as an alone entity.