• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2022.06a
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• pp.36-38
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• 2022

차세대 VTS 통합 플랫폼 개발 연구사업은 클라우드 기반의 플랫폼 구축을 통해 해상교통관제센터에서 수집한 관제정보를 중앙에서 통합하여 관제 및 유관 기관에 선박교통 안전과 관련된 정보를 제공하는 시스템 개발을 목적으로 하고 있다. 이에 따라 본연구에 구축될 IaaS 클라우드 플랫폼을 쉽고 효율적이며 안정적으로 운영할 수 있는 통합운영 관리 시스템이 필요하며 연구과제 특성 및 요구사항 분석을 통해 최적화된 통합운영관리 시스템을 개발하였다.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.189-195
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• 2007

Helicteres isora is medicinally important plant effective against asthma, diabetes, hypolipidemia, HIV, besides a good source of diosgenin. Seed dormancy and low rate of natural fruit production make this plant a perfect candidate for developing an in vitro method useful for its clonal propagation and further biotechnological developments. This is the first report on in vitro production of this plant. Nodal explants obtained from aseptically germinated seedlings were cultured on MS medium (Murashige and Skoog 1962) fortified with indole-3-acetic acid (IAA) ($0.57-22.83\;{\mu}M$), indole-3-butyric acid (IBA) ($0.41-16.58\;{\mu}M$), 6-benzylaminopurine (BA) ($0.44-17.75\;{\mu}M$) and kinetin (Kin) ($0.46-13.94\;{\mu}M$) either singly or in combinations of IAA + BA, IAA + Kin and BA + Kin. Combinations of cytokinins (BA and Kin) were most suitable for multiple shoot induction and $13.94\;{\mu}M\;Kin\;+\;13.31\;{\mu}M\;BA$ was optimum (79% frequency) associated with high number of microshoots (7.1 shoots per explant) after 20 days of culture. Maximum shoot elongation and proliferation (10 shoots per explant with 4.8 cm average height) was achieved on MS media containing $2.32\;{\mu}M\;Kin\;+\;2.22\;{\mu}M\;BA\;+\;2.85\;{\mu}M\;IAA$. High rooting frequency (70%) was achieved on MS medium (1/2 basal strength) fortified with $4.14\;{\mu}M$ IBA, while activated charcoal showed inhibitory effects on rooting. Hardening was done with 76% survival rate and these plants were growing without any visual defects and morphologically mimicking the naturally growing plants.

• Korean Journal of Medicinal Crop Science
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• v.20 no.3
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• pp.190-194
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• 2012

Shoot tips of chinese yam (Dioscorea opposita Thunb.) were cultured on MS medium containing 0.5 mg/L BA to produce micro-tubers in vitro. To stimulate the formation of shoots and micro-tubers, and produce large micro-tubers, the sections of micro-tubers were cultured on MS media with BA and IAA. The shoot multiplication, and the micro-tuber formation and growth were very effective on the media containing 2.0 mg/L BA and 0.5~1.0 mg/L IAA. Sucrose added to MS medium with 2.0 mg/L BA and 0.5 mg/L IAA to stimulate more micro-tuber growth. The medium added 50 g/L sucrose was very effective in the increase of plant fresh weight and micro-tuber growth. After 4 weeks' culture of micro-tuber sections on the medium with 2.0 mg/L BA, 0.5 mg/L IAA and 50 g/L sucrose, the liquid media were added into the same vessels. The micro-tuber growth was stimulated remarkably by the addition of liquid medium. The addition of 25 $m{\ell}$ liquid medium containing 10 g/L activated charcoal, 3x MS salts and 250 g/L sucrose was the most effective in micro-tuber growth.

• Journal of Plant Biology
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• v.35 no.4
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• pp.371-383
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• 1992

The effects of IAA $(10^{-3}M)\;an;GA_3\;(2{\times}10^{-8}\;M)$ on the biosynthesis of phospholipids and their fatty acid compositions in chloroplast isolated from Chlorella ellipsidea were analyzed. Growth ratio and the levels of total lipids and phospholipids in whole cell system and chloroplast treated with phytohormones were higher than those of control, and when treated with GAl. phosphatidylcholine content was predominantly higher than that of control. It was showed that in whole cell system the fatty acid utilized for biosynthesis of phopholipids was 27.43% for palmitic acid in control, while that was 20.25% for linolenic acid in treatment with 1M. In treatment with $GA_3$, 23.17% for linolenic acid was used in phospholipid formation. The major fatty acid in the chloroplst system was analyzed to be 35.67% for palmitic acid in control and in treatment with 1M, 24.91% for linolenic acid was used in phospholipid formation, while in treatment with $GA_3$, major fatty acid of phospholipids was 22.80% for linoleic acid.c acid.

• KSBB Journal
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• v.31 no.3
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• pp.158-164
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• 2016

Plant growth promoting (PGP) hormones, which are produced in a small quantity by bacteria, affect in plant growth and development. PGPs play an important role on the crop productivity in agricultural field. In this study, a photosynthetic bacterial strain producing the PGP was isolated from paddy soil. Bacterial isolate was gram negative, rod-shaped and motility positive. From the 16s rRNA gene sequence analysis, the isolate was identified as Rhodobacter capsulatus PS-2. The mass cultivation of R. capsulatus PS-2 was optimized by considering of the carbon, nitrogen and inorganic salt sources. Optimal medium composition was determined as Na-succinate 4.5 g, yeast extract 5 g, $K_2HPO_4$ 1 g, $MgSO_4$ 5 g, per liter. From the result of 500 L fermentation for 2 days using the optimal medium, the viable cells were $8.7{\times}10^9cfu/mL$. R. capsulatus PS-2 strain produced the carotenoid and indole-3-acetic acid (IAA). The carotenoid extraction and quantitative analysis were performed by HCl-assisting method. Total carotenoid contents from R. capsulatus PS-2 culture broth were measured as $7.02{\pm}0.04$ and $6.93{\pm}0.05mg/L$ under photoheterotrophic and chemoheterotrophic conditions, respectively. To measure the productivity of IAA, colorimetric method was employed using Salkowski reagent at optical density 535 nm. The results showed that the highest content of IAA was $197.44{\pm}5.92mg/L$ in the optimal medium supplemented with 0.3% tryptophan.

• Korean Journal of Food Science and Technology
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• v.16 no.4
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• pp.392-397
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• 1984

These experiments were conducted to investigate general enzymatic characteristics of two forms(glucoamylase I and glucoamylase II) of the purified glucoamylase produced by Rhizopus oryzae. Molecular weights of glucoamylase I and glucoamylase II estimated by Sephadex G-100gel filtration, were approximately 101,000 and 115,000, respectively, and those estimated by SDS-polyacrylamide gel electrophoresis being 120,000 and 127,000, respectively. Isoelectric points of the above enzyme were pH 7.25 and pH 7.75. The optimum temperature was $50^{\circ}C$ and the enzyme was stable below $45^{\circ}C$. Optimum pH of both glucoamylase I and glucoamylase II was about pH 5.0. The stable pH range of them were pH 3.5-8.0 and 4.5-8.0, respectively. Michaelis constants of glucoamylase I and glucoamylase II toward souluble starch were 4.545 mg/ml and 5.560 mg/ml, respectively. $Hg^{++}$, $Pb^{++}$, p-CMB and IAA were inhibitors of glucoamylase I and $Hg^{++}$, $Mn^{++}$, p-CMB and IAA were inhibitors of glucoamylase II.

• Applied Biological Chemistry
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• v.34 no.2
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• pp.142-148
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• 1991

When cultured soybean immature seed on 15th days after flowering, suitable temperature in formation of callus were $24{\sim}27^{\circ}C$, and embryogenic callus(EC) were generated at medium containing NAA with growth regulators, and then, results were subcultured the EC, a plenty of shoots and roots were formed at medium supplemented BA 2mg/1 and IAA 2mg/1, respectively, however when used at medium the same time supplemented BA 2mg/1 and IAA 2mg/1, formation of cullus was energetic, and a symptom of organization was not showed , Total lipid contents include in each cultures were increased at low temperature of cultural conditions as much as possible, but glycolipid, phospholipid, free sterol contents were a little increased at $24{\sim}27^{\circ}C$, and free sterol content was increased at a case of embryogenic structure were generated. In fatty acid compositions in each cultures, the contents of unsaturated fatty acid were plenty in EC, and unsaturation rate was 0.837. Besides, in sterol compositions, cholesterol content was remarkably high in EC than that of other cultures.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.81-84
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• 2004

Branched broomrape (Orobanche ramosa) is a holo-parasitic flowering plant that attaches to the root of its host, green plant, by means of a specialized structure known as haustorium. Following successful contact and penetration on susceptible plant root, complex tissue of Orobanche cells is formed which is known as the tubercle. Newly formed tubercles contain high activity ofindole-3-acetic acid (IAA). Triiodobenzoic acid (TIBA), as an inhibitor of IAA polar transport, was utilized to investigate the supply and requirement of auxin to the developing O. ramosa on tomato plant. There was no significant reduction in the incidence of O. ramosa per pot of different TIBA treatments. However, infection severity in terms of the number of O. ramosa shoots that emerged per plant and number of attachments per plant root system were significantly reduced by 60 % and 45 % on TIBA treated plants, respectively. Histo-logical studies revealed conspicuous delay in the initiation of xylem vessel differentiation inside tubercles of the TIBA treated tomato plants. Also, differentiated vessels showed thinner secondary wall deposition, and improper alignment within bundles inside those tubercles. They were wider and shorter in diameter in comparison to those of untreated plants. These findings were attri-buted to the short supply of IAA required for normal development, and to the xylem vessel differentiation of O. ramosa tubercles on infected tomato. Hence, this parasitic flowering plant seems to depend upon its host in its requirements for IAA, in a source to sink relation-ship.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.455-461
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• 1987

The ovaries or the ovules of grasses were pollinated and cultured in vitro to raise the interspecific or the intergeneric hybrids between tall fescue, meadow fescue, and Italian ryegrass. The isolated and suface-sterili-zed pistils were dusted with compatible pollens on stigma, on stump after removing stigma, or on excised ovule. Furthermore, the fertilized ovaries and ovules were cultured on MS, M6, or White's media and treated with plant growth regulators: IAA, kinetin, BA to promote embryo development and seed maturity. The in vitro fertilization in grass species ranged from 44 to 92% depending on ovary and pollen parents. The stigmatic pollination was resulted in 67.8% fertilization, the stump pollination 89.0%, and the excised ovule pollination 61.0%, repectively. White's medium was the most effective to provide embryo development and seed maturity in grass species. And the combined treatment of IAA 10mg/$\ell$, kinetin 0.2mg/$\ell$, was better than the non-treatment. Only two seedlings, one complete and one abnormal with root formation were obtained from 127 ovaryies cultured. The anatomy of ovules in vitro cultured was revealed the differentiation of vascular system and meristematic tissue, and the formation of sclerenchyma cells inside ovule.

• Journal of Life Science
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• v.21 no.7
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• pp.969-975
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• 2011

We focused on relationship between phytohormones and AtEXPA3 gene in gravitropic response of A. thaliana. RT-PCR analysis shows that AtEXPA3 was highly expressed in actively developing tissues such as leaf, rosette, root and flower tissues. AtEXPA3 gene expression was enhanced by gravistimulation, BR and IAA. Furthermore, decreased gravitropism was observed when treatment of AVG, an ethylene biosynthetic inhibitor, suggesting that ethylene has a gravistimulating effect itself as well as BRs and IAA. Inhibition of gravitropism in AtEXPA3 RNAi mutant suggests that BR, auxin and ethylene are playing roles as regulators of AtEXPA3. In addition, altered gravitropism in BRs signaling mutant (decreased in bri1-301, bak1, and increased BRI-GFP) indicated that BRs signaling mediated the gravitropism. In conclusion, gravitropic responses of Arabidopsis root resulting from root growth were mediated by increased expression of AtEXPA3 gene, which is stimulated by phytohormones.