• Biomolecules & Therapeutics
• /
• 제10권1호
• /
• pp.37-42
• /
• 2002

Brain drug targeting through the blood-brain barrier (BBB) in vivo is possible with peptidornirnetic monoclonal antibodies that undergo receptor-mediated transcytosis through the BBB. Monoclonal antibody to the rat transferrin receptor, such as the OX26 was studied in rats as a transport vector through BBB on the transferrin receptor. But, OX26 is not an effective brain delivery vector in mouse. In the present studies, rat monoclonal antibody, 8D3 to the mouse transferrin receptor were evaluated for brain drug targeting vector intransgenic mouse model. Pharrnacokinetic parameters in plasma and organ uptakes were determined at varioustimes after i.v. bolus injection of [$^{}125}I$] 8D3 in Balb/c mice. Brain uptake of [$^{}125}I$] 8D3 was also studied with an internal carotid artery perfusioncapillary depletion method. After i.v. injection of [$^{}125}I$] 8D3, plasma concentrations declined biexponentially with elimination half lift of approximately 2.2 hours. Brain uptake of [$^{}125}I$] 8D3 was $0.50{\pm}0.09$ persent of injected dose per g brain after 2 hours i.v. injection. After perfusion 5 min the apparent volume of distibution of [$^{}125}I$] 8D3 in brain was $22.3 {\mu}l/g,$ which was 4.8 fold higher than the intravascular volume. These studies indicate rat monoclonal antibody to the mouse transferrin receptor, 8D3 may be used for brain drug targeting vector in mice.

• 한국수자원학회논문집
• /
• 제34권6호
• /
• pp.577-586
• /
• 2001

본 연구에서는 모형의 구조가 상대적으로 간단한 구형펄스모형을 이용하여 I-D-F 곡선을 유도할 수 있는 이론적 방법론을 제시하였다. 강우모형의 구조를 고려하여 유도되는 I-D-F 곡선은 관측 강우의 1차원 및 2타원 통계 특성을 이용하여 추정된 매개변수에 의해 그 형태가 결정되므로 년최대치계열을 이용하여 추정하는 1-D-F 곡선에 비해 비정상적인 강우사상에 상대적으로 덜 민감하게 된다. 본 연구는 서울 및 인천지점에 적용되었으며 이때. 유도 된 I-D-F 곡선은 년최대치계열을 이용하여 유도된 I-D-F 곡선과 비교함으로서 그 적용성을 판단해 보았다. 본 연구의 결과를 요약하면 다음과 같다: (1) 지속기간이 길어짐에 따라 중첩확률은 아주 크게 증가한다. 그러나, 중첩에 따른 강우강도의 증가정도는 지속기간이 증가함에 따라 완만하게 감소하는 추세를 나타내고 있다. (2) 중첩을 고려하는 경우, 특히 지속기간 및 재현기간이 긴 경우에, 강우강도의 증가가 두드러짐을 확인할 수 있었다. 이는 깅 우강도의 계산 시 추정된 중첩확률과 재현기간을 함께 고려함으로 생기는 당연한 결과이다. 아울러, 서울과 인천지점의 비교에서는 서울지점의 경우가 중첩의 효과가 더욱 크게 나타나고 있음을 확인할 수 있었으며, 이는 추정된 중첩확률의 차이, 보다 궁극적으로는 구형펄스모형의 매개변수의 차이로 설명될 수 있다. (3) 본 연구에서 사용한 구형펄스 모형은 강우의 군집특성을 고려하지 못함으로 유도된 I-D-F 곡선도 전체적으로 년최대치를 이용한 I-D-F 곡선에 비해 작은 강우강도를 나타내었었다. 그러나 각 곡선의 전체적인 형태는 유사함을 확인할 수 있었으며 강우의 군집특성을 고려하는 강우모형을 사용할 경우 보다 나은 결과를 유도할 수 있을 것으로 판단된다.

• Biomolecules & Therapeutics
• /
• 제28권3호
• /
• pp.272-281
• /
• 2020

Environmental agents, including viral and bacterial infectious agents, are involved in the alteration of physicochemical and biological parameters in the nasal epithelium. Hyaluronan (HA) has an important role in the regulation of tissue healing properties. High molecular weight HA (HMW-HA) shows greater anti-inflammatory responses than medium molecular weight HA (MMW-HA) and low molecular weight HA (LMW-HA). We investigated the effect of HMW-HA, MMW-HA and LMW-HA on the regulation of physicochemical and biological parameters in an "in vitro" model that might mimic viral infections of the nasal epithelium. Human nasal epithelial cell line RPMI2650 was stimulated with double-stranded RNA (dsRNA) Poly(I:C) for 5 days in air-liquid-interface (ALI) culture (3D model of airway tissue). dsRNA Poly(I:C) treatment significantly decreased transepithelial electrical resistance (TEER) in the stratified nasal epithelium of RPMI2650 and increased pH values, rheological parameters (elastic G' and viscous G''), and Muc5AC and Muc5B production in the apical wash of ALI culture of RPMI2650 in comparison to untreated cells. RPMI2650 treated with dsRNA Poly(I:C) in the presence of HMW-HA showed lower pH values, Muc5AC and Muc5B production, and rheological parameters, as well as increased TEER values in ALI culture, compared to cells treated with Poly(I:C) alone or pretreated with LMW-HA and MMW-HA. Our 3D "in vitro" model of epithelium suggests that HMW-HA might be a coadjuvant in the pharmacological treatment of viral infections, allowing for the control of some physicochemical and biological properties affecting the epithelial barrier of the nose during infection.

• Journal of applied mathematics & informatics
• /
• 제34권5_6호
• /
• pp.369-382
• /
• 2016

In this paper, we investigate the global behavior of the solutions of the difference equation $x_{n+1}=\frac{A+Bx_{n-2k-1}}{C+D\prod_{i=l}^{k}x_{n-2i}^{m_i}}$, n=0, 1, ..., with non-negative initial conditions, the parameters A, B are non-negative real numbers, C, D are positive real numbers, k, l are fixed non-negative integers such that l ≤ k, and m_i, i=l, k are positive integers.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2005년도 추계 학술대회논문집(수송기계편)
• /
• pp.105-108
• /
• 2005

The radiated noise from the automotive intake system should be predicted at the design stage. To this end, the precise measurement of in-duct acoustic source parameters of the intake system, i.e., the source strength and source impedance, is essential. Most of previous works on the measurement of acoustic source parameters were performed under a fixed engine speed condition. However, the requirement of vehicle manufacturer is the noise radiation pattern as a function of engine speed. In this study, the direct method was employed to measure the source parameters of engine intake system under a fixed engine speed and engine run-up condition. It was noted that the frequency spectra of source impedance hardly changes with varying the engine speed. Thus, it is reasonable to calculate the source strength under the engine run-up condition by assuming that source impedance is invariant with engine speed. Measured and conventional source models, i.e., constant pressure source, constant velocity source, and non-reflective source, were utilized to predict insertion loss and radiated sound pressure level. A reasonable prediction accuracy of radiated sound pressure level spectra from the intake system was given in the test vehicle when using the measured source characteristics which were acquired under the operating condition.

• 대한화학회지
• /
• 제30권3호
• /
• pp.273-281
• /
• 1986

본 연구는 임피던스 파라미터(전기이중층 내의 미분용량 $C_d$, 용핵저향 $R_Q$, 전하이동저항 $R_t$,및 흡착유사용량 $C{\phi}$)를 결정하기 위해 정확한 측정식을 얻는데 주력하낟. 정현파가 정상상태에 있을 때 복소수함수 ${\omega}$중의 허수부분만을 으용하여 구한 전체임피던스식은 낮은 주파수에서 $|Z_{LF}|=1/{\omega}_{1C\phi}{\sqrt{1+{{\omega}_1}^2/{\omega}^2}$에, 높은 주파수에서는 $|Z_{HF}|={\omega}_2/({\omega}_1{\omega}_3C{\phi})({\omega}^2+{{\omega}_2}^2){\sqrt{{({\omega}^2+{\omega}_2{\omega}_3)}^2+{({\omega}_2{\omega}-{\omega_3{\omega})^2}}$에 따름을 나타낸다. 이들 임피던스 파라미터의 계산에 필요한 전체임피던수, 위상각 및 쎌전류값을 .5M $NA_2SO_4$ 또는 1M NaCl, 19.373% 해수, 1M HCl, 1M $KNO_3$ 지지전해질과 이들의 지지전해질내에 각각 $10^{-2}M Kl$ 또는 6mM DBNA를 첨가하여 20~60Hz 범위에서 실험적으로 측정하였다. 본 연구에서 유도한 식은 임피던스 파라미터 $C_d,\;C_{\phi},\;R_t$ 및 $R_Q$의 값들이 각각 $15{\sim}4{\mu}F/cm^2,\;162{\sim}758{\mu}F/cm^2,\;11.5{\sim}57.6ohm{\cdot}cm^2$ 및 $0.5{\times}10^{-2}{\sim}4.1{\sim}10^{-2}\;ohm{\cdot}cm^2$임을 보여준다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권4호
• /
• pp.568-578
• /
• 2020

Objective: The objective of this study was to investigate effects of supplementation of culture media from solid-state fermented Isaria cicadae (I. cicadae) on performance, serum biochemical parameters, serum immune indexes, antioxidant capacity and meat quality of broiler chickens. Methods: A total of 648 Arbor Acres male broiler chickens(1 d; average body weight, 42.93±0.47 g) were randomly assigned to 6 treatments, each with six replicates and 18 broiler chickens per replicate. Broiler chickens were fed phase I (d 1 to 21) and phase II (d 22 to 42) diets. The phase I diets were corn and soybean-meal based diets supplemented with 0%, 2%, 4%, 6%, 8%, or 10% culture media from solid-state fermented I. cicadae respectively. The phase II diets were corn and soybean-meal based diets supplemented with 0%, 1.33%, 2.67%, 4.00%, 5.32%, or 6.67% culture media from solid-state fermented I. cicadae respectively. Results: In phase I, the broiler chickens with the supplementation of culture media had increased body weight gain and feed intake (linear and quadratic, p<0.05) with increasing inclusion of culture media. The levels of serum total antioxidant capacity (T-AOC), glutathione (GSH) and superoxide dismutase (SOD) increased linearly (p<0.05). In phase II, levels of serum T-AOC and interleukin-1β increased linearly (p<0.05), and GSH increased (p<0.05). In the kidney, GSH and glutathione peroxidase (GSH-Px) concentrations increased (linear and quadratic, p<0.05) and SOD concentration increased linearly (p<0.05). Compared to the control, shear force and drip loss of breast muscle decreased (linear and quadratic, p<0.05). Drip loss of leg muscle decreased linearly and quadratically (p<0.05). Conclusion: Dietary supplementation of culture media from solid-state fermented I.cicadae which was enriched in both wheat and residual bioactive components of I. cicadae enhanced the growth performance of broiler chickens. It also improved body anti-oxidative status and contributed to improve broiler meat quality.

• Toxicological Research
• /
• 제32권4호
• /
• pp.317-325
• /
• 2016

Increased access to highly active antiretroviral therapy (HAART) has made the management of drug toxicities an increasingly crucial component of HIV. This study investigated the effects of adjuvant use of coconut oil and HAART on testicular morphology and seminal parameters in Sprague-Dawley rats. Twelve adult male Sprague-Dawley rats, weighing 153~169 g were distributed into four groups (A-D) and treated as follows: A served as control (distilled water); B (HAART cocktail-Zidovudine, Lamivudine and Nevirapine); C (HAART + Virgin coconut oil 10 mL/kg) and D (Virgin coconut oil 10 mL/kg). After 56 days of treatment, animals were killed and laparotomy to exercise the epididymis for seminal fluid analyses done whilst testicular tissues were processed for histo-morphometric studies. Result showed a significant decline in sperm motility (P < 0.05) and count (P < 0.0001) in HAART-treated animals while there was insignificant changes in other parameters in groups C and D except count that was reduced (P < 0.0001) when compared with controls. Histomorphological studies showed HAART caused disorders in seminiferous tubular architecture with significant (P < 0.01) decline in epithelial height closely mirrored by extensive reticulin framework and positive PAS cells. Adjuvant Virgin coconut oil + HAART resulted in significant decrease in seminiferous tubular diameter (P < 0.05), but other morphometric and histological parameters were similar to control or Virgin coconut oil alone (which showed normal histoarchitecture levels). While derangements in testicular and seminal fluid parameters occurred following HAART, adjuvant treatment with Virgin coconut oil restored the distortions emanating thereof.

• 대한의용생체공학회:의공학회지
• /
• 제29권3호
• /
• pp.212-221
• /
• 2008

The total hip replacement (THR) has been used as the most effective way to restore the function of damaged hip joint. However, various factors have caused some side effects after the THR. Unfortunately, the success of the THR have been decided only by the proficiency of surgeons so far. Hence, It is necessary to find the way to minimize the side effect caused by those factors. The purpose of this study was to suggest the definite data, which can be used to design and choose the optimal hip implant. Using finite element analysis (FEA), the biomechanical condition of bone cement was evaluated. Stress patterns were analyzed in three conditions: cement mantle, procimal femur and stem-cement contact surface. Additionally, micro-motion was analyzed in the stem-cement contact surface. The 3-D femur model was reconstructed from 2-D computerized tomography (CT) images. Raw CT images were preprocessed by image processing technique (i.e. edge detection). In this study, automated edge detection system was created by MATLAB coding for effective and rapid image processing. The 3-D femur model was reconstructed based on anatomical parameters. The stem shape was designed using that parameters. The analysis of the finite element models was performed with the variation of parameters. The biomechanical influence of each parameter was analyzed and derived optimal parameters. Moreover, the results of FE A using commercial stem model (Zimmer's V erSys) were similar to the results of stem model that was used in this study. Through the study, the improved designs and optimal factors for clinical application were suggested. We expect that the results can suggest solutions to minimize various side effects.

• 천문학회지
• /
• 제39권3호
• /
• pp.57-71
• /
• 2006

We investigated the structural parameters of a sample of 30 dwarf galaxies(15 dEs and 15 dS0s) in the Virgo Cluster using i-band images from the Sloan Digital Sky Survey Data Release 4. Among 28 galaxies for which surface brightness profiles were derived from ellipse fittings, 23 galaxies had a single component that was adequately described by a generalized $S\acute{e}rsic$ function with a shape parameter ranging from n=0.5 to 2, while 5 galaxies(2 dEs and 3 dS0s) had bulge and disk components that were fitted by a generalized $S\acute{e}rsic$ function and an exponential function, respectively. Since the majority of dwarf galaxies in the present sample had a single component, it seems likely that genuine dS0 galaxies that have disk and bulge components are quite rare in the Virgo Cluster. The similarity in structural parameters of genuine dS0 galaxies in the Virgo Cluster with those of Magellanic-type galaxies implies that the progenitors of dwarf lenticular galaxies in the Virgo Cluster were most likely Magellanic-type galaxies if dS0s are harassed late-type spirals.