• Journal of Plant Biology
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• v.31 no.4
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• pp.289-298
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• 1988

The pattern of elongation in the developing hypocotyl of Glycine max shows that the elongation generally proceeds from base to the cotyledonary node in acropetal diredtion, although earlier elongation takes place through the entire hypocotyl. Because the differentiation of the vascular cambium in the hypocotyl advances also acropetally, it can be seen that the acropetal wave of hypocotyl elongation is associated with the acropetal differentiation of the cambium in the hypocotyl elongation is associated with the acropetal differentiation of the cambium in the hypocotyl elongation is associated with the acropetal differentiation of the cambium in the hypocotyl. The elongation of procambial cells occurs not only during active elongation but also after cessation of elongation of the hypocotyl. In tangential view, the procambium of the hypocotyl in early stage has homogeneous structure composed of short cells. Subsequently, these procambial cells elongate actively and then become elongated long cells. These long cells eventually become fusiform initials, while some of elongated long cells are transversely divided and then converted into ray initials. The characteristics of the vascular cambium are entirely acquired some time after hypocotyl elongation is completed, and the transitin from procambium to vascular cambium in the hypocotyl is a rather gradual process.

• Journal of Plant Biology
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• v.34 no.4
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• pp.274-281
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• 1991

The relationship between elongation of hypocotyl and its vascular transition was studied with seedling of Glycine max. the hypocotyl elongation proceeded acropetally from the base of hypocotyl toward the cotyledonary node. The vascular transition did not occur in the basal region of the hypocotyl, which did not nearly elongate, with exarch radial vascular bundles. However, the vascular transition was almost completed at the middle part of the hypocoty, more or less elongated, with endarch collateral vascular bundles. Such bundles also appeared in the uppermost region of the hypocotyl, in which the elongation was the most striking. These results suggested that the vascular transition was related to the hypocotyl elongation and that the transition of primary vascular system in Glycine max seedling was established by rather rapid process. Our observations of the serial sections from root to hypocotyl revealed that the vascular system through the root-hypocotyl-cotyledon was a unit, to which one of the epicotyl that did not participate in transition was superimposed.

• Molecules and Cells
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• v.45 no.4
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• pp.243-256
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• 2022

Transcriptional regulation, a core component of gene regulatory networks, plays a key role in controlling individual organism's growth and development. To understand how plants modulate cellular processes for growth and development, the identification and characterization of gene regulatory networks are of importance. The SHORT-ROOT (SHR) transcription factor is known for its role in cell divisions in Arabidopsis (Arabidopsis thaliana). However, whether SHR is involved in hypocotyl cell elongation remains unknown. Here, we reveal that SHR controls hypocotyl cell elongation via the transcriptional regulation of XTH18, XTH22, and XTH24, which encode cell wall remodeling enzymes called xyloglucan endotransglucosylase/hydrolases (XTHs). Interestingly, SHR activates transcription of the XTH genes, independently of its partner SCARECROW (SCR), which is different from the known mode of action. In addition, overexpression of the XTH genes can promote cell elongation in the etiolated hypocotyl. Moreover, confinement of SHR protein in the stele still induces cell elongation, despite the aberrant organization in the hypocotyl ground tissue. Therefore, it is likely that SHR-mediated growth is uncoupled from SHR-mediated radial patterning in the etiolated hypocotyl. Our findings also suggest that intertissue communication between stele and endodermis plays a role in coordinating hypocotyl cell elongation of the Arabidopsis seedling. Taken together, our study identifies SHR as a new crucial regulator that is necessary for cell elongation in the etiolated hypocotyl.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.634-639
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• 1996

This experiment was conducted to evaluate the variability of hypocotyl elongation of mungbean varieties. With four mungbean cultivars, which were classified as 4234-697 and Keumsungnogdu(long), Nampyungnogdu(Medium), and Seonhwanogdu(short), hypocotyl elong-ation was measured 4 to 6 days after seeding in paper towel at different temperatures (15, 20, 25, 30, and 35$^{\circ}C$). Hypocotyl elongation of mungbean seed stored at 5$\pm$1$^{\circ}C$ for 6 months was compared with of seed stored at room temperature. As the temperature rises, the hypocotyl is longer. The hypocotyl elongation started immediately at high temperature, and longest at the range of 30 to 35$^{\circ}C$. The hypocotyl elongation became longer at the 5$^{\circ}C$ storage plot than at the room temperature plot. Correlation coefficient (r) between 100 seed weight and hypocotyllength are not significant.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.1
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• pp.68-77
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• 1992

The present experiments were conducted to investigate the variability of hypocotyl elongation among the major soybean varieties by checking several conditions. The results obtained are summarized as follows. The rate of hypocotyl elongation is the highest during the day from 3.0 to 3.5 after seeding. It follows that it may be reasonable to evaluate the hypocotyl elongation of soybean seeds by comparison of hypocotyl length. And the tested 15 major cultivars could be classified as follow ; long ; Eunhakong, Janggyungkong and Bokwangkong, medium ; Namhekong, Dangyung-kong, Danyubkong, Milyangkong, Dugyukong, Paldalkong, Mangunjoseng, Namchunkong and Seal kong, short ; Gwanggyo, Begunkong and Jangbegkong. The hypocotyl elongation in small seed is longer than large seed. Correlation coefficients(r) for the relationships between 100 seed weight and hypocotyl elongation is -0.2506$^{**}$. As the rising temperature, the hypocotyl length is elongated, and longest at the range of 30 to 35$^{\circ}C$. The effects G $A_3$ hastened the hypocotyl elongation of soybean seed, and ABA, Kinetin and BA inhibit it, and that of those in short hypocotyl cultivars are higher than long hypocotyl cultivars. Hypocotyl length of long hypocotyl cultivars are longer than that of short hypocotyl cultivars under high temperature pre -treatment.

• Journal of Plant Biology
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• v.11 no.1
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• pp.22-26
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• 1968

Hypocotyl segments of shade-grown soybean sprouts(var. Kumdo) were floated in solutions of various substances at physiological levels and grown for either 24 hours at 28$^{\circ}C$ or 72 hours at $25^{\circ}C$. Increased length of the segments beyond 20mm (the original length) was obtained as a measure of the present studies. At the room temperature, Ca and DNP strongly inhibited elongation of the hypocotyl segments, whereas K, IAA, GA and EDTA in general promoted it. There were, however, no such differences in the effects at relatiely low temperature. This indicated that the elongation process not only involves stretching of wall materials, but also does synthesis of the materials. Ca was found to be antagonistic to the promotive action of GA in the elongation, and the IAA action involves metabolic energy. EDTA seemed to act as a widely known chelator removing Ca already existed in the hypocotyl tissue, thus shwoed a promotion in the elongation.

• Journal of Integrative Natural Science
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• v.1 no.1
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• pp.24-31
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• 2008

Brassinosteroids (BRs) are a special class of plant steroid hormones that are essential for normal growth and development. Part of confusion is whether BRs are unique to plants, because they have overlapping physiological roles with other better-studied hormones and with physiological responses caused by light. In systems designed to assay for cytokinins, the effects of BRs vary. We measured hypocotyl length for testing the ability of brassinolide (BL) to rescue double mutant between det2 and the photoreceptor null mutant phytochrome B (phyB). PHYB involved in controlling hypocotyl elongation in increased concentration of BL whereas phyBdet2 double mutant just partially rescue to phyB in white and red light indicated the involvement of BRs in PHYB regulated cell elongation. BRs regulated hypocotyl growth was delayed by BAP, a cytokinin treatment but inhibitory effects of BAPs on hypocotyl growth was slightly recovered by BL. The result indicated that the mode of action of BR and cytokinin is independent or sequential in the downstream light-regulated response control on hypocotyl elongation and also light modulated the action of BR and cytokinin in some extent.

• Journal of Plant Biology
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• v.27 no.2
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• pp.43-50
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• 1984

Effects of IAA on the elongation and cell wall hlysocidase activities were investigated in excised rape (Brassica napus L. cv. Yongdang) hypocotyl segments. IAA promoted the elongation of rape hypocotyl segments. In rape hypocotyls, the first 10-mm segments from the hook exhibited maximal elongation and the capacity of elongation was gradually decreased with increasing distance of each 10-mm from the hook. A good correlation has been obtained between the magnitude of endogenous growth and the activities of $\alpha$, $\beta$-glucosidase and $\alpha$, $\beta$-galactosidase. However, exogenous application of IAA did not seem to enhance the tissue with IAA resulted in acidification of the incubation medium. From these data, we can conclude that IAA seems to enhance elongation of the tissue segments, at least in part, by releasing hydrogen ion into cell wall, some of which may participate in the cell wall extension process, but does not seem to trigger the activation of $\alpha$, $\beta$-glucosidase and $\alpha$, $\beta$-galactosidase.

• Journal of Plant Biology
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• v.32 no.4
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• pp.305-312
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• 1989

Developmental anatomy was conducted in order to elucidate the differentiating pattern of fascicular cambial initials in the hypocotyl of Ricinus communis. The homogeneous procambium with relatively short cells in early stage is transformed into a heterogeneous structure with long and short cells in late stage in tangential view. Fusiform and ray initials are gradually originated from the long and short cells of the procambium in hypocotyl in later stage respectively. Fusiform initials are not shorter than procambial cells because of the successive elongation of vascular meristematic cells. Therefore, the distinction between procambium and fascicular cambium is not made from comparison with their cell length. The characteristics of the fascicular cambium are gradully acquired at or just after completion of hypocotyl elongation.

• Journal of Plant Biology
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• v.32 no.4
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• pp.323-330
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• 1989

This work was carried out to elucidate effects of endogenous and exogenous IAA transport on adventitious root formation in Phaseolus vulgaris hypocotyl cuttings. For inverted or normal incubation in distilled water, the adventitious root is always formed at the morphological base but not at the morphological apex. For inverted incubation, in both distilled water and certain chemical solution, the root formation is retarded more at the first stage (0-24 hr) than at the second stage (24-48 hr). When p-chlorophenoxyisobutyric acid (PCIB) was applied to the cuttings at the first stage, theroot formation was inhibited more than at the second stage. Treatment of 2,3,5-triiodobenzoic aicd (TIBA)markedly inhibited the adventitious root formation in Phaseolus vulgaris hypocotyl cuttings. This inhibition influenced the root according to the applied stage and period. Therefore, the root formation is more related to the stage of root primordium formation than to the stage of root elongation from the primordium. Inhibition of auxin transport oraction by TIBA or PCIB could also be reversed when hypocotyl cuttings are incubated in exogenously applied IAA solution.