• Title/Summary/Keyword: Hyperthermophilic Archaeon

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Ammonia Production from Yeast Extract and Its Effect on Growth of the Hyperthermophilic Archaeon Sulfolobus solfataricus

  • Park, Chan-Beum;Lee, Sun-Bok
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.3 no.2
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    • pp.115-118
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    • 1998
  • Utilization of yeast extract and formation of byproduct metabolite were investigated for hyperthermophilic archaeon Sulfolobus solfataricus (DSH 1617). In both batch and fed-batch cultivations of S. solfataricus, maximal cell density, {{{{ { NH}`_{4 } ^{ +} }}} ion production and pH change were highly dependent on the ratio of yeast extract to glucose in the medium. Variation of {{{{ { NH}`_{4 } ^{ +} }}}} ion level was identified as a major cause of pH change during cultivation, and acidification of culture broth was attributed to consumption of {{{{ { NH}`_{4 } ^{ +} }}}} ions rather than formation of acid byproducts. It was also observed that increase of {{{{ { NH}`_{4 } ^{ +} }}}} ion concentrations in the medium resulted in greater degree of growth inhibition.

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Purification and Structural Characterization of P93 Complex from Hyperthermophilic Archaeon Thermococcus profundus (초고온 archaeon인 Thermococcus profundus에서 P93 복합체의 분리 및 구조적 특성)

  • Lee, Mi-Hong;Kim, Suk-Kyoung;Yun, Young-Gun;Park, Seong-Cheol;Bahk, Jeong-Dong;Cheong, Gang-Won
    • Applied Microscopy
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    • v.30 no.2
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    • pp.185-191
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    • 2000
  • An unusually large protein complex was found in the cytosol of the hyperthmophilic archaeon. Thermococcus profundus. The purified protein was shown to be a homomultimer of 93 kDa subunit (P93 complex). The complex is extremely heat stable. During 12 hrs incubation with SDS (final concentration 1%) at $85^{\circ}C$, no changed structure could be observed. Electron image analysis of negatively stained showed that the complex has a single, stable characteristic view and a well-preserved core with threefold rotational symmetry. The periphery of the assembly is composed of a nebulose, possibly flexible, component. Based on the projected structure suggest the P93 complex from T. profundus is composed 24 homomultimer.

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Thermosome-like Protein from Hyperthermophilic Archaeon Thermococcus Profundus; Purification and Structural Analysis (초고온 Archaeon인 Thermococcus profundus로부터 Thermosome유사 단백질의 분리 및 구조 분석)

  • Kim, Suk-Kyoung;Lee, Mi-Hong;Park, Seong-Cheol;Cheong, Gang-Won
    • Applied Microscopy
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    • v.30 no.4
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    • pp.413-421
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    • 2000
  • We have isolated a large cylindrical protein complex from hyperthermophile archaeon Thermococcus profundus. Structural analysis by image processing of electron micrographs suggests that the complex is composed of two stacked rings of eight subunits each; the ring enclose a central channel. The purified protein was shown to be a homomultimer of 60 kDa subunit (P60 complex). It exhibits an extremely thermostable ATPase activity with a temperature optimum of $80^{\circ}C$. This protein complex may play an important role in the adaptation of thermophile archaeon to life at high temperature.

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Statistical Optimization of Medium for Formate-driven Bio-hydrogen Production by the Hyperthermophilic Archaeon, Thermococus onnurineus (초고온성 고세균 Thermococcus onnurineus의 개미산으로부터 바이오수소 생산을 위한 통계적 배지 최적화)

  • Lee, Sung-Mok;Kim, Tae Wan;Lee, Hyun Sook;Lee, Jung-Hyun;Kang, Sung Gyun
    • Ocean and Polar Research
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    • v.39 no.4
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    • pp.269-277
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    • 2017
  • Medium compositions for the hyperthermophilic archaeon, Thermococcus onnurineus NA1 was statistically optimized to enhance formate-driven hydrogen ($H_2$) production by using response surface methodology. From the Plackett-Burman design-based experiment, it was confirmed that among the minor components of medium such as KCl, $MgSO_4$, $NH_4Cl$, Cystein-HCl, trace elements, Fe-EDTA and $CaCl_2$, the trace elements were screened as the only positively effective components with respect to $H_2$ production. Subsequently, the optimal concentrations of the trace elements and the major components of a medium such as NaCl, yeast extract and sodium formate were determined from the five-level central composite design (CCD)-based experiment. The resulting quadratic model predicted the maximum $H_2$ production of 46.6 mmol/L in serum bottle and it was validated experimentally using the optimal medium initially supplemented with 26.70 g/L of NaCl, 9.81 g/L of sodium formate, 3.50 g/L of yeast extract and 4.59 mL/L of trace elements. From the duplicate batch cultivations in the fermentor using the optimized medium, the a maximum $H_2$ production rate up to 71.8 mmol/L/h could be obtained, which was a 65% enhanced value compared with that obtained using the control medium, showing the high efficiency of the optimized medium.

Cultivation of the Hyperthermophilic Archaeon Sulfolobus solfataricus in Low-Salt Media

  • Park, Chan-Beum;Lee, Sun-Bok
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.4 no.1
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    • pp.21-25
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    • 1999
  • Two low-salt complex media, bactopeptone and desalted yeast extract, were used for high density cultivation of the hyperthermophilic archaeon Sulfolobus solfataricus (DSM 1617). Bactopeptone, which has low mineral ion content among various complex media, was good for cell growth in batch cultures; the maximal cell density in bactopeptone was comparable to that in yeast extract. However, cell growth was rather poor when bactopeptone was added by the fed-batch procedure. Since several vitamins are deficient in abctopeptone, the effect of vitamins on cell growth was examined. Among the vitamins tested, pyridoxine was found to improve the growth rate of S. solfataricus. To reduce the growth inhibition caused by mineral ions, yeast extract was dialyzed against distilled water and then fed-batch cultures were carried out using a fed medium containing desalted yeast extract. Although the concentrations of mineral ions in yeast extract were significantly lowered by the dialysis whether low molecular weight solutes in yest extract are crucial for cell growth, we investigated the effect of trehalose, a most abundant compatible solute in yeast extract, on the growth pattern. Cell densities were increased and the length of the lag phase was markedly shortened by the presence of trehalose, indicating that trehalose plays an important role in the growth of S. solfataricus.

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