• Archives of Pharmacal Research
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• v.27 no.12
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• pp.1253-1257
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• 2004

6-Methoxydihydrosanguinarine (6ME), a benzophenanthridine alkaloid derived from the methanol extracts of Hylomecon hylomeconoides, showed a dose-dependent effect at 1-10 ${\mu}M$ on causing apoptotic cell death in HT29 colon carcinoma cells $(IC_{50} = 5.0{\pm}0.2 {\mu}M)$. Treatment of HT-29 cells with 6ME resulted in the formation of internucleosomal DNA fragmentation. Treatment of the cells with 6ME caused activation of caspase-3, -8 and 9 protease and subsequent proteolytic cleavage of poly(ADP-ribose)polymerase. 6ME increased the expression of p53 and Bax and decreased the expression of Bid. These results indicate that p53 and proapoptotic Bcl-2 family proteins might participate in the antiproliferative activity of 6ME in HT29 cells.

• Archives of Pharmacal Research
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• v.26 no.2
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• pp.114-119
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• 2003

A high performance liquid chromatographic (HPLC) method was developed for the qualitative and quantitative determination of benzophenanthridine alkaloids from the methanol extracts of Hylomecon hylomeconoides and H. vernale (Papaveraceae). Achiral and chiral methods were adapted for the separation of 6-methoxydihydrosanguinarine (1), 6-acetonyldihydrosanguinarine (2) and dihydrosanguinarine (3). The achiral reversed phase HPLC method made it possible the simultaneous separation and determination of 1, 2 and 3 within 20 min on ODS column using acetonitrile-phosphate buffer (50 mM, pH 7.0) (50 : 50, v/v). The separation and determination of 1 and 2 enantiomers was available using chiral columns. The same amount of (+) and (-)-enantiomers of 1 was found from the methanol extract of specimen, indicated that 1 could be the artifact produced by the reaction of sanguinarine with methanol. H. hylomeconoides showed higher level of 1 and 3 in compared with H. vernale, especially in root samples permitting the possibility of chemical discrimination between two species.

• Korean Journal of Environment and Ecology
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• v.17 no.2
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• pp.112-122
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• 2003

This study was carried out to offer the raw data for the conservation of plant ecosystem in Chungcheongbuk-do with systematic grasping the vascular plant in Mt. Midong and to establish the accurate countermeasure of conservation on the rare species as well as to restore their habitats. The vascular plant species in Mt. Midong were summarized as 303 taxa; 81 families, 215 genus, 262 species, 38 varieties and 3 forms. The rare plants designated by Korea Forestry Service were two taxa; Polygonatum stenophyllum, Hylomecon hylomeconoides. The nine taxa were recorded in the surveyed sites as the endemic plants; Melandryum seoulense, Hylomecon hylomeconoides, Spiraea prunifolia for. simpliciflora, Filipendula palmata var. glabra, Ajuga spectabilis, Weigela subsessilis, Lonicera coreana, Cirsium setidens and Saussurea seoulensis. And the naturalized plants were twelve taxa; Rumex crispus, Lepidium apetalum, Robinia pseudo-acacia, Tyifolium pratense, Trifolium repens, Hibiscus trionum, Oenothera odorata, Ambrosia artemisifolia var. elatior, Erigeron annuus, Erigeron canadensis, Taraxacum officinale and Sonchus oleraceus.

• Korean Journal of Environment and Ecology
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• v.17 no.3
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• pp.210-223
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• 2003

This study was carried out to investigate the flora of Mt. Baekwoon. The flora of Mt. Baekwoon was surveyed from October, 2000 to August, 2001. In the results of survey, the vascular plant species in Mt. Baekwoon were summarized as 468 taxa; 90 families, 272 genera, 411 species, 1 subspecies 49 varieties and 7 forms. The rare plants designated by Korea Forestry Service were nine species; Lilium distichum, Gastrodia elata, Viola diamantica, Hylomecon hylomeconoides, Aristolochia contorta, Asarum maculatum, Rodgersia podophyllu, Viola albida, Scopolia japonica and Scrophularia koraiensis. And the endemic plants were nineteen species; Asarum maculatum, Melandryum seoulense, Clematis trichotoma, Aconitum pseudo-laeve var. erectum, Hylomecon hylomeconoides, Chrysosplenium barbatum, Spiraea prunifolia for. simpliciflora, Robus hongnoensis, Vicia venosa var. cuspidata, Tilia taquetii, Viola seoulensis, Viola diamantica, Ajuga spectubilis, Scrophularia koraiensis, Weigela subsessilis, Adenophora remotiflora, Cirsium setidens, Saussurea macrolepis and Saussurea seoulensis.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.122-134
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• 2004

This study was carried out to investigate the resource plants of Mt. Midong. The resource plant of Mt. Midong was surveyed from March, 2002 to October, 2003. In the result of survey, the vascular plant species in Mt. Midong were summarized as 400 taxa; 85 families, 266 genera, 349 species, 48 varieties and 3 forms. And in the results of survey on resource plants among 400 taxa, we confirmed 162 taxa of ornamental plants(40.5％), 250 taxa of edible plants(62.5 ％), 257 taxa of medicinal plants(64.3％) and 167 taxa of the others(41.8 ％). The six taxa were recorded in the surveyed sites as the Korean endemic plants; Hytomecon hylomeconoides, Filipendula palmata var. glabra, Ajuga spectabilis, Weigela subsessilis, Cirsium setidens and Saussurea seoulensis. The rare and endangered plants designated by Korea Forestry Service were two taxa; Hylomecon hylomeconoides and Polygonatum stenophyllum.

• The Korean Journal of Ecology
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• v.16 no.1
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• pp.93-114
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• 1993

The vegetation of Mt. Mudeung was investigated from April, 1991 to September, 1992. The units of vegetation were classified 10 units by the Braun-Blanquet's phytosociological method. The forest vegetation was classified into 10 communities, Pinus densiflora, Pinus vigida, Chamaecyparis obtusa afforestation, Quercus mongolica, Q. variabilis, Q. serrata, Q. acutissima, Miscanthus sinensis var. purpurascens, Hylomecon hylomeconoides and Drosera rotundifolia community. Based on the classification, the actual vegetation map and degree of green naturality were drawn in 1:50,000 scale. The vertical distribution of the main component species was investigated based on the vegetation data of the EN slope and SW slope of Mt. Mudeung from altitude 200m to top.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.169-182
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• 2004

The purpose of this study is carried out accurately to grasp the vascular plants in Mt. Bakdal (825 m of sea level), Goesan-gun, Chungcheongbuk-do. In the results of survey from October, 2000 to August, 2001, the vascular plant species in Mt. Bakdal were summarized as 458 taxa; 91 families, 275 genera, 391 species, 62 varieties and 5 forms. The rare plants designated by Korea Forestry Service were six species; Lilium distichum, Iris uniflora var. carisina, Hylomecon hylomeconoides, Viola albida, Scopolia japonicus and Scrophularia koraiensis. And the endemic plants were seventeen species; Melandryum seoulense, Aconitum pseudo-laeve var. erectum, Hylomecon hylomeconoides, Spiraea prunifolia for. simpliciflora, Rubus hongnoensis, Gleditsia japonica var. koraiensis, Lespedeza ${\times}$ tomentella, Vicia venosa var. cuspidata, Vicia venosissima, Tilia taquetii, Ajuga spectabilis, Paulownia coreana, Scrophularia koraiensis, Weigela subsessilis, Lonicera subsessilis, Cirsium setidens and Saussurea seoulensis. The sixteen species were recorded in the surveyed sites as the naturalized plants; Rumex acetocella, Rumex crispus, Fallopia dumetora, Chenopodium glaucum, Amaranthus retroflexus, Robinia pseudo-acacia, Amorpha fruticosa, Trifolium repens, Abutilon avicennae, Hibiscus trionum, Oenothera odorata, Oenothera lamarckiana, Erigeron annuus, Erigeron canadensis, Bidens frondosa and Carduus crispus.

• Korean Journal of Environmental Biology
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• v.31 no.2
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• pp.121-153
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• 2013

We investigated the flora of Mudeung-san (Mt.) located in Gwangju-gwangyeoksi, Hwasun-gun and Damyang-gun and discussed the vascular plants found there. 22 days separate fild trips were completed from March of 2009 to November 2011. As a result, we identified the distribution of 816 taxa in Mudeung-san (Mt.), comprising 123 families, 441 genera, 668 species, 5 subspecies, 121 varieties and 18 forms. There are a total 111 taxa of floristic regional indicator plants. The floristic regional indicator plants are as follows; nine taxa of level V such as Gastrodia elata Blume, six taxa of level IV such as Hylomecon hylomeconoides T. Lee, 21 taxa of level III such as Stewartia korena Nakai, 18 taxa of level II, and 66 taxa of level I. In addition, two critically endangered species (CR), Galeola septentrionalis Rchb.f. and Paeonia obovata Maxim., two endangered species (EN), Dryopteris cycadina (Franch. & Sav.) C.Chr. and Jeffersonia dubia Benth., and eight vulnerable species (VU), such as Lilium callosum Siebold & Zucc, were found in the investigated area. Also, 14 endermic plants of Korea, such as Salix koriyanagi Kimura and Aconitium chiisanense Nakai, were found.

• Korean Journal of Plant Taxonomy
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• v.50 no.1
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• pp.27-55
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• 2020

We investigated the flora of Ibamsan Mt. and Baekamsan Mt., and discuss here important vascular plants found on these mountains. We created a plant list using voucher specimens collected from Ibamsan Mt. and Baekamsan deposited at CNU. There are 769 taxa in Ibamsan Mt. and Baekamsan Mt., consisting of 116 families, 403 genera, 687 species, 16 subspecies, 58 varieties, and 8 forms. Twenty-four endemic plants of Korea were found, including Coreanomecon hylomeconoides Nakai and Eranthis byunsanensis B. Y. Sun. There are 15 rare plants on Ibamsan Mt. and Baekamsan as designated by the Korea Forest Service, including Iris koreana Nakai and Chamaegastrodia shikokiana Makino & F. Maek., as well as 14 red-list plants as designated by the Ministry of the Environment, including Lycoris chinensis var. sinuolata K. Tae & S. C. Ko ex K. Tae & S. C. Ko and Cymbidium macrorhizon Lindl. In total, there are 133 taxa of floristic regional indicator plants, including four level V taxa, such as Iris koreana Nakai; ten level IV taxa, such as Lindera sericea (Siebold & Zucc.) Blume; 28 level III taxa, such as Botrychium japonicum (Prantl) Underw.; 22 level II taxa, including Hylomecon vernalis Maxim.; and 69 level I taxa, including Dryopteris erythrosora (D. C. Eaton) Kuntze. There are 37 invasive plants, including Rumex acetosella L. and Symphyotrichum pilosum (Willd.) G. L. Nesom.