• Animal cells and systems
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• v.4 no.1
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• pp.95-100
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• 2000

The regional distribution and relative frequencies of endocrine cells were studied immunogistochemically (PAP methods) in the alimentary tract of the tree frog, Hyla areorea japonica, using specific antisera against serotonin, somatostatin, bovine Sp-1/chromogranin (BCG), cholecystokinin (CCK)-8, vasoactive intestinal polypeptide (VIP), gastrin, bombesin, secretin and pancreatic polypeptide (PP). Six kinds of endocrine cells were identified in this study, These immunoreactive cells were located in the gastric glands of stomach regions and in the basal portion of the epithelium of the intestinal tract or esophagus with variable frequencies. They were spherical or spindle-shaped. Serotonin-immunoreactive cells were observed in the whole alimentary tract including the esophagus. Somatostatin-immunoreactive cells were also detected throughout the alimentary tract except the rectum. CCK-8-immunoreactive cells were observed from the pylorus to ileum. Vip-immunoreactive cells were restricted to the rectum. Bombesin-immunoreactive cells were restricted to the fundic gastric regions and gastrin-immunoreactive cells were restricted to the pylorus. However, no BCG-, secretin and PP-immunoreactive cells were demonstrated in this study. In conclusion, the regional distribution and relative frequency of the endocrine cells in the alimentary tract of the tree frog were similar to other anuran species, but some differences which may be caused by feeding habits and species specification were also observed.

• Animal cells and systems
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• v.3 no.2
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• pp.193-197
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• 1999

A peroxidase-antiperoxidase method was used to detect the cells showing immunoreactivities to six hormone antibodies in the alimentary tracts of six frog species, Rana nigromaculata, R. rugosa, R. amurensis coreana, R. catesbeiana, Bombina orientalis, and Hyla arborea japonica, inhabiting Korea. The cells immunoreactive to gastrin and cholecystokinin-8 were observed in the pylorus of the stomachs and in the small intestines of all frog species examined. In contrast, these somatostatin-immunoreactive cells were identified in the esophagus and the whole gastrointestinal tracts, but were absent from the large intestines in R. rugosa, R. catesbeiana, B. orientalis and H. arborea japonica. The pancreatic polypeptide (PP)-immunoreactive cells represented their distribution limited to the small intestines of R. amurensis coreana and H. arborea japonica, and they were additionally identified in the pylorus of the stomachs in the other four species. Serotonin- and glucagon- Immunoreactive cells revealed different regional distributions in which the former were observed throughout the whole alimentary tracts in all frog species investigated, whereas the latter were not found in these regions at all. Endocrine cells were relatively abundant in the pyloric portion of the stomach compared to other organs. The present study showed that all endocrine cells except for PP had a similar distribution in the alimentary tracts of all frog species used.

• The Korean Journal of Zoology
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• v.5 no.1
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• pp.35-38
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• 1962

Two subspecies are known to Family Hylidae(Order Anura) in Korea : namely , Hylaarborea japonica GUENTHER and H.a.stepheni BOULENGER, and they have been hitherto distinguished as different subspecies from four characteristics : the rate of interorbital to incternasal length, the rate of diameter of tympanum to diameter of 3 rd finger disc, the rate of the length of inner metatarsal tubercle to diameter of 3 rd finger disc, and the rate of inner metatarsal tubercle to the length of lst toe. The author has compared the above to subspecies for the characteristics with 123 individuals collected from ten different localities in Korea and has found that their fluctation curves overlap each other. The author considers, therefore, that the four characteristics could not be assumed as keys for the classification of the two subspecies and has reached the conclusion that these two subspecies should be regarded as one subspecies and Hyla arborea japonica GUENTHER should be given to both of them as the subspecies name.

• The Korean Journal of Pharmacology
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• v.11 no.1 s.17
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• pp.15-18
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• 1975

The effect of skin extracts of Korean amphibian, poisonous snake and fresh-water fish were determined for their caerulein-like action on rabbit gall bladder strips. The isolated gall bladder strips were prepared according to the technique described by Amer and Becvar(1969). The strips were placed in a bath containing 100ml of Locke-Ringer solution maintained at $38^{\circ}C$. Oxygen was continuously bubbled through the solution. The tension of the muscle strip was initially adjusted to 0.7g. The contractile response was measured isometrically by a force-displacement transducer connected to a polygraph. In this rabbit gall bladder strip caerulein produced contraction of CCK-PZ type. The skin extract of Korean amphibian also elicited similar contraction as caerulein, which extracted from Australian amphibian, Hyla caerulea, by Erspamer et al. The calculated amount was approximately $2{\mu}g$ caerulein per gram of skin tissue in Korean amphibian and the potency was about 1/200 of that seen in Australian amphibian. The contraction of gall bladder strip by our amphibians occurs in decreasing order; Rana Nigromaculata coreana Okada, Rana nigromaculata Hallowell, Hyla arborea japonica Gunther and Bombina orientalis Boulenger. The skin extracts of poisonous snake and fresh-water fish produced no caerulein-like activity.

• The Korean Journal of Zoology
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• v.3 no.2
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• pp.13-18
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• 1960

For the study on the sex ratio in natural populations of the different localities, the amphibian species were collected as the materials at the seven localities in Korea, and were obtained the following results. The sex ratios of each amphibian species suggest that each has it's own specific sex rations the following list. Rana nigromaculata H. : 109-39, Rana rugosa SCH. : 67.44, Rana temporaria coreana O. : 158.45, Hyla arborea japonica G. : 137.70. In Rana nigromaculata H. the average sex ratio was 109.39 as the above list, and its was reached statistically significant level according to the different localiies. The sex ratio was generally higher in the eastern area than that in the western area in this country. The sex ratio of the adult frogs of Rana nigromaculata H. was 135.18 , and it has a statistically significant level by each locality but the sex ratio of the younger frogs of the same species was 100.97 which was a little lower than that in the adult, and it was not laid at a significant level according to the localities. There is no doubt that the difference of sex ratios according to the localities was mainly due to the different ratios of the adult frogs which were effected on the constitution of sex by environmental factors in each locality.