• Journal of fish pathology
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• v.20 no.2
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• pp.179-187
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• 2007

Hydrolysis compound of cactus of opuntia genus, Opuntia ficus-indica var. saboten Makino, has predominant anti-bacterial, anti-fungal effects. In this study, we wont to know a preventive effects of bacterial disease on cultured flounder using the compound (MBT-01108), and physical and physiological responses of those fishes were examined additionally. The MBT-01108 shown any side effect on physical, hematological and histological conditions of fishes. Immunologically, CL (chemiluminescense) response were appeared at MBT-01108 (0.1%) feeding group for 4 weeks. The results of comparative livability at a experimental infection with E.tarda and S.iniae were 82.5% and 13.5% at 4 weeks MBT (0.1%) feeding group.

• Journal of fish pathology
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• v.21 no.1
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• pp.29-34
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• 2008

In this study, we examined in vitro antimicrobial activity of the hydrolysis compound (MBT-01108) of cactus stem (Opuntia ficus-indica var. saboten Makino) against 18 strains of fish pathogenic bacteria, Edwardsiella tarda, Listonella anguillarum, Vibrio sp., Lactococcus garvieae, L. raffinose, Streptococcus parauberis and S. iniae. Antibiotic susceptibility of the same bacteria was also assayed to commercial discs containing various antibacterials. According to these results, MBT-01108 showed the excellent antimicrobial activity against the tested pathogenic bacteria.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.915-922
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• 2007

In order to discover physiologically active substance, we investigated a powder obtained by processing of Opuntia ficus-indica var. saboten Makino trunk. The powder was treated by sulfuric acid and then extracted by several solvents such as methanol, methylene chloride, ethanol etc. Among them, the best antimicrobial activity was showed by methylene chloride extract. To identify materials exhibiting physiological activation, the acid hydrolyzed extract was separated by 7 fractions through preparative silica gel TLC. The effective fraction exhibiting the best broad antimicrobial activity was identified, named as MBT-01108. From structural analysis of the products extracted to acid hydrolysis, a compound exhibiting the antimicrobial activities is identified to levulinic acid. Levulinic acid isolated from Opuntia ficus-indica var. saboten Makino may be applicable as a natural preservative of food or cosmetic and for prevention of bacterial diseases, an ingredient of acne, ageing and whitening cosmetics and an antimicrobial agent.