• Asian-Australasian Journal of Animal Sciences
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• v.28 no.8
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• pp.1202-1208
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• 2015

The effects of daily dietary Bacillus subtilis (Bs), and adding L-tryptophan, fructan, or casein to fecal fermentation broths were investigated as means to reduce the production of noxious gas during manure fermentation caused by ammonia, hydrogen sulfide ($H_2S$), and 3-methylindole (skatole). Eighty swine ($50.0{\pm}0.5kg$) were equally apportioned to an experimental group given Bs in daily feed, or a control group without Bs. After 6 weeks, fresh manure was collected from both groups for fermentation studies using a $3{\times}3$ orthogonal array, in which tryptophan, casein, and fructan were added at various concentrations. After fermentation, the ammonia, $H_2S$, L-tryptophan, skatole, and microflora were measured. In both groups, L-tryptophan was the principle additive increasing skatole production, with significant correlation (r = 0.9992). L-tryptophan had no effect on the production of ammonia, $H_2S$, or skatole in animals fed Bs. In both groups, fructan was the principle additive that reduced $H_2S$ production (r = 0.9981). Fructan and Bs significantly interacted in $H_2S$ production (p = 0.014). Casein was the principle additive affecting the concentration of ammonia, only in the control group. Casein and Bs significantly interacted in ammonia production (p = 0.039). The predominant bacteria were Bacillus spp. CWBI B1434 (26%) in the control group, and Streptococcus alactolyticus AF201899 (36%) in the experimental group. In summary, daily dietary Bs reduced ammonia production during fecal fermentation. Lessening L-tryptophan and increasing fructan in the fermentation broth reduced skatole and $H_2S$.

• Transactions of the Korean hydrogen and new energy society
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• v.24 no.4
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• pp.275-281
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• 2013

This study was conducted to increase the amount of bio-hydrogen production from microalgae(Chlorella vulgaris) in batch reactors by thermal pre-treatment. The optimization of thermal pre-treatment was conducted using statistic experimental design of response surface methodology. Two experimental parameters of temperature and reaction time were considered. The optimization condition was founded at the coded variables of <0.52, -0.07> corresponding to the experimental of heating temperature of $95.6^{\circ}C$ and reaction time of 57.9 min, respectively. Under the optimal condition, the maximum hydrogen production was predicted to 25.3mL $H_2/g$ dry cell weight (dcw), which was 9.1 times higher value of control(2.8mL $H_2/g$ dcw).

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.5
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• pp.1049-1054
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• 2009

The effect of Sacchromyces cerevisiae-Fermented Sophorae Radix water extract (SFS) on production of hydrogen peroxide and nitric oxide (NO) from mouse macrophage Raw 264.7 Cells treated with nicotine (1 mM) was investigated through this study. SFS (0, 25, 50, 100, 200, 400 ug/mL) was simultaneously treated with nicotine (1 mM) during culture of 4, 20, 24, 44, 48, 68, and 72 hr. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. NO production after 24 hr treatement was measured with Griess reagent assay. SFS restored the production of hydrogen peroxide and NO reduced by nicotine (1 mM) in Raw 264.7 Cells. These results suggests that SFS could be supposed to have the immunological activity concerned with macrophage's oxidative burst including hydrogen peroxide and NO.

• Transactions of the Korean hydrogen and new energy society
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• v.21 no.1
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• pp.12-18
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• 2010

Photobiological hydrogen production using Rhodobacter sphaeroides KD131 was studied on the effect of light intensities and nitrogen sources. Media containing malate and glutamate were shown higher hydrogen production rate than that containing succinate and $(NH_4)_2SO_4$ at the $110\;W/m^2$ illumination by halogen lamp at $30^{\circ}C$. Media lacking glutamate as the nitrogen source exhibited higher hydrogen production than that containing glutamate. Initial cell concentration was optimized to 1.0 at the absorbance of 660 nm. Hydrogen production was increased by increasing the light intensity from 0 to $216\;W/m^2$ but the increasing rate declined over $108\;W/m^2$.

• KSBB Journal
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• v.6 no.4
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• pp.351-361
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• 1991

This study is on the investigation of hydrogen production and substrate removal by photosynthetic bacteria. After using of Rhodospillum rubrum KS-301 and IFO 3986, which are photosynthetic bacteria as strains, R. rubrum KS-301 was turned out a better strain. And result of experiment in which glucose and sodium lactate, components of wastewater, were used limiting substrates, showed that the productivity of hydrogen was indifferent with the kind of substrates. In batch experiments using free cells and immobilized whole cells, the decrease in hydrogen productivity was observed in the latter case. From the results of these experiments, specific growth rate of cells, specific utilization rate of glucose, and specific production rate of hydrogen were calculated. And each rate was expressed in the form of Monod equation of which parameters were estimated. Also the optimum condition of hydrogen production for free cells was $30^{\circ}C$, pH 7, and 12,000 Lux, and the optimum immobilized condition was as follows: initial immobilized cell concentration 1.0g/L, sodium alginate concentration 2% and light intensity 12,000 Lux.

• Journal of Microbiology
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• v.38 no.2
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• pp.99-104
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• 2000

Hydrogen peroxide has been used in cleaning the piping of an advanced high-purity water system that supplies ultra-high purity water (UHPW) for 16 megabyte DRAM semiconductor manufacturing. The level of hydrogen peroxide-resistant bacteria in UHPW water was monitored prior to and after disinfecting the piping with hydrogen peroxide. Most of the bacteria isolated after hydrogen peroxide disinfection were highly resistant to hydrogen peroxide. However, the percentage of resistant bacteria decreased with time. The hydrogen peroxide-resistant bacteria were identified as Micrococcus luteus, Bacillus cereus, Alcaligenes latus, Xanthomonas sp. and Flavobacterium indologenes. The susceptibility of the bacteria to hydrogen peroxide was tested as either planktonic cells or attached cells on glass. Attached bacteria as the biofilm on glass exhibited increased hydrogen peroxide resistnace, with the resistance increasing with respect to the age of the biofilm regrowth on piping after hydrogen peroxide treatment. In order to optimize the cleaning strategy for piping of the high-purity water system, the disinfecting effect of hydrogen preoxide and peracetic acid on the bacteria was evaluated. The combined use of hydrogen peroxide and peracetic acid was very effective in killing attached bacteria as well as planktonic bacteria.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.2
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• pp.438-442
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• 2009

The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Lactobacillus pentosus (AFL) on hydrogen peroxide production within mouse macrophage Raw 264.7 Cells treated with gallic acid, EtOH, Nicotine, Acetaminophen, and Acetaldehyde. AFL (0${\sim}$400 ug/mL) was treated with gallic acid, EtOH, Nicotine, Acetaminophen, and Acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFL showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, Nicotine, Acetaminophen in Raw 264.7 Cells. AFL could be supposed to have the immunological activity related with macrophage's oxidative burst.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.419-422
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• 2002

Fermentative hydrogen production by Citrobacter sp. Y 19 was investigated in batch culture. Optimal hydrogen production activity was observed at pH 6 - 7 and temperature of $36^{\circ}C$, and hydrogen yield and maximal hydrogen production rate were 1.12 mmol/mmol glucose and 32.3 mmol/g cell${\cdot}$h, respectively. With glucose as a substrate, the bacterium produced ethanol, acetate, and carbon dioxide as major glucose fermentation by-products. Y19 could utilize various sugars such as galactose, fructose, lactose, sucrose, and starch for cell growth and hydrogen production.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.3
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• pp.608-612
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• 2009

The effect of Sacchromyces cerevisiae-Fermented Artemisiae Argi Folium Water extract (AFS) on hydrogen peroxide production within mouse macrophage Raw 264.7 Cells treated with EtOH, gallic acid, Nicotine, Acetaminophen, and Acetaldehyde was investigated through this study. AFS (0-400 ug/mL) was simultaneously treated with EtOH, gallic acid, Nicotine, Acetaminophen, and Acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFS restorated the intracellular productions of hydrogen peroxide reduced by EtOH, gallic acid, Nicotine, Acetaminophen within Raw 264.7 Cells. AFS could be supposed to have the immunological activity concerned with macrophage's oxidative burst.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.1
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• pp.96-101
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• 2010

The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Sacchromyces cerevisiae (AFS) on hydrogen peroxide production within human hepatocyte HepG2 cells treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. AFS (0~400 ug/mL) was treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFS showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde in HepG2 Cells. AFS could be supposed to have the hepatoprotective effect related with hepatocytologic signaling activity against gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.