• Membrane and Water Treatment
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• v.10 no.3
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• pp.191-199
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• 2019

In this study, we investigated the cultivation possibility using Hydrodictyon reticulatum in a continuous raceway pond as a tertiary sewage treatment plant. The cultivation possibility was evaluated by varying the light quantity, wavelength, and hydraulic retention time (HRT). Experimental results showed that the growth rates of algae and the removal efficiencies of nutrients increased as the light quantity increased, and the maximum photosynthetic rate was maintained at $100{\mu}mol/m^2{\cdot}s$ or higher. When wavelength was varied, nutrient removal efficiency and growth rate increased in the following order: green light, red light, white light, and blue light. The nutrient removal efficiencies and algae productivity in HRT 4 d were better than in HRT 8 d. We conclude that if Hydrodictyon reticulatum is cultivated in a raceway pond and used as a tertiary treatment facility in a sewage treatment plant, nutrients can be effectively removed, and production costs can be reduced.

• Korean Journal of Weed Science
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• v.13 no.1
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• pp.1-6
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• 1993

The experiments were conducted in order to find out the response of algae as affected by fertilizer and salinity, and to determine the chemical control method of algae in 1991. The higher the nitrogen fertilizer level, the more the occurrence of a blue-green alga Oscillatoria concerning with soil flakes. An increase in phosphorus fertilizer level stimulated the growth of green alga Hydrodictyon, whereas an increase in nitrogen level caused its abrupt reduction due to the harmful effect at high concentration of nitrogen, The high level of nitrogen fertilizer also reduced the growth of Euglena, although the detrimental effect was less compared with that of Hydrodictyon. A blue-green alga Oscillatoria, a green alga Cladophora and a diatom Navicula were tolerant to salinity. Piperophos/dimethametryn was highly effective in controlling various algae such as a green alga Hydrodictyon, suspended unicellular green algae and Euglena species. A fungicide propineb and copper sulfate also effectively controlled Hydrodictyon.

• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1112-1119
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• 2017

The aim of this study was to develop a potential process for bioethanol production from Hydrodictyon reticulatum (HR), a filamentous freshwater alga, using Saccharomyces cerevisiae (KCTC7017). From the sugar solutions prepared by the four different hydrolysis methods, bioethanol production ranged from 11.0 g/100 g dried material (acid hydrolysis) to 22.3 g/100 g dried material (enzymatic hydrolysis, EH). Bioethanol was fermented from a highly concentrated sugar solution obtained by a decompression-mediated (vacuum) enrichment method (VE). As the results, ethanol was more efficiently produced from HR when sugar solutions were concentrated by VE following EH (EH/VE). Using multiple feeding of the sugar solution prepared by EH/VE from HR, ethanol reached up to a concentration of 54.3 g/l, corresponding to 24.9 g/100 g dried material, which attained the economic level of product concentration (approximately 5%). The results indicate that by using HR, it is feasible to establish a bioethanol production process, which is effective for using microalgae as the raw material for ethanol production.

• Korean Journal of Weed Science
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• v.12 no.4
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• pp.335-351
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• 1992

The identification of green algae which were collected from paddy rice fields of the whole nation was conducted in 1991, and also the reproduction processes of three important green algae were investigated. The green algae identified were 46 species in 16 families including 4 species in the Volvocaceae, 4 species in the Palmellaceae, 3 species in the Ulotrichaceae, 3 species in the Oocystaceae and 6 species in the Scenedesmaceae. It was recognized that the algae which have caused a large injury to the flooded direct-seeded rice in Seosan reclaimed saline land was the genus Cladophora. The green water blooms on paddy water were induced mostly by the concentrated populations of suspended green algae such as the genera, Chlamydomonas and Stichococcus. A rapid propagation of Hydrodictyon was resulted from the asexual reproduction forming autocolony in each mother cell. The sexual and asexual reproduction processes of Oedogonium and the sexual reproduction process of Spirogyra were observed.

• ALGAE
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• v.22 no.3
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• pp.167-183
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• 2007

Fifty-six taxa of chlorococcales were recorded from different water bodies of eastern and north-eastern states of India. These belong to 21 genera, e.g. Chlorococcum (1), Truebaria (1), Pediastrum (9), Hydrodictyon (1), Botrycoccus (1), Coenochloris (1), Radiococcus (1), Coenocystis (1), Oocystis (1), Glaucocystis (1), Chlorella (1), Kirchneria (2), Kirchnereilla (1) Ankistrodesmus (10), Coelastrum (3), Actinastrum (2), Tetrastrum (1), Crucigenia (1), Crucigeniella (1) Desmodesmus (6) and Scenedesmus (9). All these species were recorded first time from this region and out of these 16 species reported first from India.

• Korean Journal of Weed Science
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• v.32 no.2
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• pp.85-97
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• 2012

To investigate the usefulness of freshwater alga Water-net (Hydrodictyon reticulatum, HR) as resources for production of fermentable sugars, the easiness of enzymatic saccharification was evaluated at first. When 6 plant materials (HR, Spirulina, Chlorella, Scenedesmus, Cladophora, Corn stover) were enzymatically hydrolyzed with 2% solid loading at the same condition, HR showed the highest ratio of saccharification based on glucose production. No milled HR was also completely saccharified at the amounts of optimal enzyme mixture. Glucose yield was not changed though the citrate buffer strength for saccharification was decreased from 0.1 M to 0.1 mM. Only about 10% yield reduction was observed compared to that of $120^{\circ}C$ treatment when HR was enzymatically hydrolyzed at room temperature. The saccharification was normally occurred at $37^{\circ}C$ and pH 6.5 which is general growth condition of fermentable microrganisms, suggesting that HR have a biomass characteristics applicable for the simultaneous saccharification and fermentation. The saccharification was occurred by more than 70~80% of one of the best condition although the supplied enzyme amounts was reduced to 1/10 volume. And the glucose yield by enzymatic hydrolysis was not decreased by 10% HR solid loading and began to decrease at more than 15% solid contents. Above these results show that HR is an interesting algal biomass which is relatively easy to be saccharified by hydrolyzing enzymes. In addition, HR is a flilamentous alga and very easy to be collected. Therefore, HR seems to be an useful and valuable resources in the economical production of fermentable sugars for manufacture of bio-chemical products.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.176-183
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• 2013

To explore hydrolysis methods for the efficient manufacture of sugar solutions from the freshwater alga Water-net (Hydrodictyon reticulatum, HR), acid hydrolysis, enzymatic hydrolysis, and combined hydrolysis (acid followed by enzymatic hydrolysis) were investigated. In the one-step acid hydrolysis, the reaction of 8% solids content using 2% sulfuric acid at $120^{\circ}C$ for 1 hour was desirable. In this case, glucose 27.44 g 100 g $DM^{-1}$ could be obtained from the HR-d13 samples. In the two-step acid hydrolysis, the primary hydrolysis (HR powder : 72% sulfuric acid = 1 g : 1.5 mL) was carried out for 1 hour at $60^{\circ}C$, and then the secondary hydrolysis was done for 1 hour at $120^{\circ}C$ after addition of distilled water 23.5 mL. In this case, glucose 35.11 g/100 g DM could be obtained from the HR-d13 samples. In the combined hydrolysis, 25% solids content using 2% hydrochloric acid were reacted for 1 hour at $120^{\circ}C$, and then citrate buffer and hydrolysis enzyme complexes (E1 1.0 mL+E2 0.2 mL $g^{-1}$ dried matter) were added and reacted for 1 - 2 days at $50^{\circ}C$. In this case, glucose 33.5 g 100 g $DM^{-1}$ could be obtained from the HR-d23+26 samples. In conclusion, combined hydrolysis was likely to be more useful saccharification method of HR biomass at a practical level, considering the glucose productivity, generation of fermentation-inhibiting substances (hydroxyl methyl furfural, furfural), and limited use of strong acid.

• Korean Journal of Weed Science
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• v.12 no.2
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• pp.158-165
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• 1992

A survey on nation-wide distribution of fresh-water algae occurring in paddy rice fields was conducted in 1991. The algae which were collected from the whole nation were classified into a total of 54 genera including 14 genera in the blue-green algae. 29 genera in the green algae, 1 genus in the stoneworts. 3 genera in the euglenoids and 7 genera in the diatoms. The green algae and diatoms occurring in plain regions were diverse in terms of the number of genera distributed, whereas there was no regional difference in diversity of the blue-green algae and the euglenoids. Among the green algae, the suspended unicellular algae such as the genera, Chlamydomonas, Pandorina and Gonium, were widespread in plain regions, but the multicellular algae such as the genera, Spirogyra, Oedogonium, Ulothrix and Hydordictyon, were major in mountainous and attitudinal regions. The filamentous green algae such as the genera. Cladophora and Rhizoclonium, were dominant in reclaimed saline fields. The blue-green algae Oscillatoria spp. and the diatoms Navicula spp. were abundant in soil flakes.