• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.299-302
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• 2014

A freesia (Freesia hybrida Hort.) 'Shiny Bell' was developed for the cut flower in the National Institute of Horticultural Herbal Science in 2012. This hybrid was selected from a crossing between cn. 'Teresa' and 'Rossini', which is white semi-double flowering and red double flowering, respectively. in 2004. Morphological characteristics of the selected hybrid were investigated for 3 years from 2009 to 2011, and then it was named 'Shiny Bell'. 'Shiny Bell' had red color of RHS W155C, and double flower. The growth of the plant was vigorous and the average height was 109.3 cm. The average flower width was 5.8 mm, number of floret per stalk was 11.3, and stalk length was 8.5 cm. The average yield, 7.3 Branches per plant, was 3 stems per plant more than the control cultivar. The average days to first flowering of 'Shiny Bell', was 143.3 days, and it was approximately 7 days later than the control cultivar. The average yield of 'Shiny Bell' was 4.0 cormlets per plant.

• Transactions of the Korean hydrogen and new energy society
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• v.25 no.6
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• pp.561-569
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• 2014

Ceria ($CeO_2$) was used to increase the durability of the membrane in the polymer electrolyte membrane water electrolysis (PEMWE) circumstance. The sulfonated polyether ether ketone (SPEEK) as polymer matrix was prepared in the sulfonation reaction of polyether ether ketone (PEEK) to improve electrochemical characteristics. After sulfonation reaction, the organic-inorganic blended composite membranes were prepared by means of sol-gel casting method with loading the highly dispersed $CeO_2$ and Cs-substituted molybdophosphoric acid (Cs-MoPA) with cross-linking agent (tetrapropyl orthosilicate). Consequently, the composite membrane CL-SPEEK/Silane 4wt%/Cs-MoPA/Ceria(1%) showed the improved characteristics such as 82% of water content, 0.11136 S/cm of proton conductivity at $80^{\circ}C$, 55.50 MPa of tensile strength and 4.37% of breeding out of MoPA.

• International Journal of Industrial Entomology and Biomaterials
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• v.13 no.1
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• pp.7-14
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• 2006

A breeding programme was initiated during 2001 to introduce sex-limited larval markings to a productive multivoltine breed - BL67 from an inbred sex-limited line, MY1 (SL) maintained at Central Sericultural Research and Training Institute, Mysore. Introgressive hybridization, recurrent backcrossing for six generations followed by sib-mating resulted in synthesis of a new multivoltine silkworm breed BL67 (SL) with sex-limited larval markings. The new breed was studied for combining ability by crossing with eight bivoltine breeds viz., $NB_4D_2,\;CSR_2,\;CSR_2 (SL),\;CSR_2,\;CSR_4,\;CSR_8,\;CSR_{18}\;and\;CSR_{19}$. General combining ability effects of the new breed showed its superiority over the popular Pure Mysore by expressing significant GCA effects for six out of twelve characters whereas the results are on par with the original multivoltine breed. The hybrid $BL67(SL){\times}CSR2(SL)$ excelled in several quantitative characters such as pupation rate (90.2%), cocoon weight (1.97 g), cocoon shell weight (40 cg), cocoon shell ratio (20.3%), filament length (918 m), denier (2.96), raw silk percentage (14.96%) and neatness (90 p). Studies on cocoon size variability revealed that the cocoons of $BL67(SL){\times}CSR2(SL)$ were found comparatively uniform showing less standard deviation of 6.55 and co-efficient of variation of 3.91 %. The suitability of newly developed breed for easy grain age operation and commercial exploitation with promising hybrid have been discussed.

• Korean Journal of Agricultural Science
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• v.42 no.3
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• pp.159-165
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• 2015

The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops are also increasing. One of the major concern in risk assessment is the possible development of hybrids through interspecific and intergeneric crosses with related species. This study was conducted to investigate the pollinator have an influence on insect-mediated gene transfer from GM soybeans. Hybrid was induced from GM soybeans by honeybee and western flower thrips, and non-GM soybeans were used as pollen receptor. The analysis for gene-flow was conducted by herbicide selection, immunostrip test, and PCR analysis. In the result of the analysis, three hybrids were detected on the distance 15, 75, 105 cm from pollen source in western flower thrips treatment. In honeybee treatment, one hybrid was detected in the farthest distance (300 cm). These results suggested honeybee and western flower thrips have a possibility they can transfer the introduced gene from GM soybeans to non-GM soybeans.

• Journal of Crop Science and Biotechnology
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• v.10 no.3
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• pp.175-184
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• 2007

Two subspecies, japonica and indica, have been reported in rice, which differ in several ecotypic traits. However, reproductive barriers in hybrid progenies between subspecies have been major obstacles in breeding programs using inter-subspecific hybridization. As the first step to elucidate the reproductive barriers, we developed subspecies-specific(SS) STS markers in this study. A total of 765 STS primers were designed through comparing DNA sequences at every $2{\sim}3$cM interval between japonica and indica rices, which are available at Web DBs such as IRGSP, NCBI, TIGR, and GRAMENE, and tested for subspecies-specificity using 15 indica and 15 japonica varieties of diverse origin. Of them, 67 STS markers were identified as SS STS markers and their subspecies-specificity scores were estimated. The SS markers were dispersed throughout the genome along chromosomes. Of them, 64 SS markers were mapped on an RIL population derived from a Dasanbyeo(indica)/TR22183(japonica) cross. Genomic inclination of RILs was evaluated based on the genotyping with different types of markers. Association test between markers and segregation distortion revealed that segregation distortion might not be the cause of generating SS markers. The SS markers will be applicable to estimate the genomic inclination of varieties or lines and to study the differentiation of indica and japonica, and ultimately to breed true hybrid rice varieties in which desirable characters from both subspecies are recombined.

• Reproductive and Developmental Biology
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• v.30 no.4
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• pp.293-299
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• 2006

In order to generate transgenic goats expressing human growth hormone (hGH) in their mammary glands, goat ${\beta}-Casein/hGH$ hybrid gene was introduced into goat zygotes by pronuclear microinjection. DNA-injected embryos were transferred to the oviduct of recipients at 2-cell stage or to the uterus at morula/blastocyst stage after cultivation in glutathione-supplemented mSOF medium in vitro. Pregnancy and survival rate were not significantly different between 2-cell embryos and morula/blastocysts transferred to oviduct and uterus, respectively. One transgenic female goat was generated from 153 embryos survived from DNA injection. Southern blot analysis revealed that the transgenic goat harbored single-copy transgene with a partial deletion in its sequences. Despite of the partial sequence deletion, the transgene was successfully expressed hGH at the level of $72.1{\pm}15.1{\mu}g/ml$ in milk throughout lactation period, suggesting that the sequence deletion had occurred in non-essential part of the transgene for the transgene expression. Unfortunately, however, the transgene was not transmitted to her offspring during three successive breeding seasons. These results demonstrated that goat ${\beta}-casein/hGH$ gene was integrated into the transgenic goat genome in a mosaic fashion with a partial sequence deletion, which could result in a low level expression of hGH and a failure of transgene transmission.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.11
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• pp.1753-1762
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• 2019

Objective: The study was aimed to compare carcass traits, physicochemical and textural properties of meat in two different genotypes of Pekin ducks with regard to sex effect. Methods: The study involved 120 Pekin ducks: 30 males and 30 females of strain P33 (Polish native Pekin ducks) and 30 males and 30 females of Star 53 HY (commercial hybrid Pekin ducks). At 49 d of age, 48 birds (12 males and 12 females of each genotype) were selected for dissection. After the dissection, meat samples were collected to determine meat quality traits. Results: The studied Pekin ducks of different genotype showed significant differences in body weight, carcass weight, dressing percentage, as well as percentages of breast muscles, skin with subcutaneous fat, abdominal fat, neck, and remainders of eviscerated carcass with neck. Duck genotype influenced the content of crude protein, crude fat, Na, K, P, Zn, $pH_{24}$, electric conductivity ($EC_{24}$), cooking loss, $L^{\star}$, $a^{\star}$, most textural traits of breast muscle, and also Na, Mg and Fe content, $EC_{24}$, drip loss, cooking loss and $L^{\star}$, $a^{\star}$, and $b^{\star}$ colour coordinates of leg muscles. Regardless of genetic origin, males exhibited higher BW, carcass weight and carcass neck percentage, as well as lower redness, hardness, chewiness and gumminess of breast muscle compared to females. The genotype${\times}$sex interaction was significant for the crude fat content and cooking loss of breast muscle, and for the yellowness of leg muscle. Conclusion: Star 53 HY ducks are more suited for broiler production due to their higher body weight and dressing percentage. Their breast and leg meat are characterized by more beneficial chemical composition but has poorer sensory and textural properties compared to the meat of P33 ducks.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.405-412
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• 2021

Wolfiporia cocos is an important medicinal fungus that has been used in regions of Northeast Asia including Korea, Japan, and China. W. cocos is classified in Korea into two types (red bokryeong and white bokryeong) based on the internal colors (yellow orange-pale pink and white) of the sclerotium. Generally, the W. cocos type cultivated on farms produces white sclerotium. In this study, we endeavored to select strains that form sclerotium in sawdust medium using 2-way cross-breeding among two cultivated strains and three wild strains. Monospores were isolated from the fruiting bodies of cultivated and wild strains on potato dextrose agar. Thirty-nine strains of 338 hybrid strains isolated formed sclerotia with white or yellow colors upon culture for 3 months in Pinus densiflora sawdust medium. Selection for sclerotium forming strains using sawdust culture follows a very simple and easy procedure that is presented for the first time in this paper. We plan to test selected strains in the field to aid in developing new varieties for the future.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.231-235
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• 2010

'Jopung' (Avena sativa L.), a winter oat for forage use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2009. It was derived from an original cross between the $F_1$ hybrid of 'Early80' and 'PA202-210-S' and 'Walken' in 1999. Subsequent generations followed by the cross were handled in bulk and pedigree selection programs at Suwon and Yeoncheon, respectively. A line, 'SO99006-B-YB-29-Y1', was selected for earliness and good agronomic characteristics and designated as a line name of 'Gwiri65'. The line 'Gwiri65' was subsequently evaluated for earliness and forage yield in four different locations, Yesan, Iksan, Kimjae, and Jeju, from 2007 to 2009 and finally named as 'Jopung'. Over 3 years, the average forage dry matter yield of cultivar 'Jopung' harvested at milk-ripe stage was $17.3MT\;ha^{-1}$, compared with $13.6MT\;ha^{-1}$ of check cultivar 'Swan'. The silage quality of 'Jopung' was superior to the check cultivar 'Swan' in crude protein content, and was similar to the check cultivar in percent total digestible nutrients (TDN). Cultivar 'Jopung' is suitable primarily for winter planting in the southern regions of Korean peninsula.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.659-663
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• 2010

An Asiatic lily cultivar 'Yesol' was developed in 2005 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Korea. The cross was made in 1992 between Asiatic lily 'White Bird', a white colored cultivar, and 'Connecticut King', yellow colored cultivar. Preliminarily selection was done as 'A94-11' in 1994. Multiplication and bulb formation, and characteristic tests were conducted from 1996 to 2004. The evaluation of this line was performed and named as 'Wongyo C1-27' in 2005 that was registered as 'Yesol' to the registration office of Korea Seed & Variety Service. 'Yesol' flowers at the end of June and grows average 32.4 cm. It flowers upward-facing, yellow (RHS, Y7D) with the size of flower 14.7 cm. The average petal length and width are 8.2 cm and 2.6 cm, respectively. Leaves are 4.8 cm long and 2 cm wide. The weight and size of bulb are 18.9 g and 11.8 cm, respectively. Year-round flowering can be obtained by storing the bulb under -1.5$^{\circ}C$ conditions.