• Journal of Power Electronics
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• v.17 no.5
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• pp.1327-1337
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• 2017

Photovoltaic (PV) systems are influenced by disproportionate impacts on energy production caused by frequent mismatch cases. The occurrence of multiple maximum power points (MPPs) adds complexity to the tracking process in various PV systems. However, current maximum-power point tracking (MPPT) techniques exhibit limited performance. This paper introduces an enhanced simulated annealing (ESA)-based GMPPT technique against multiple MPP issues in P-V curve with different PV system structures. The proposed technique not only distinguishes global and local MPPs but also performs rapid convergence speed and high tracking accuracy of irradiance changing and restart capability detection. Moreover, the proposed global maximum power tracking algorithm can be applied in the central converter of DMPPT and hybrid PV system to meet various application scenarios. Its effectiveness is verified by simulation and test results.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.643-643
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• 2012

Increasing energy prices and growing concerns about global warming address the need to improve energy self-sufficiency in many industrial and municipal sectors. Wastewater treatment plants (WWTPs) are representative of energy-consuming facilities in Korea, accounting for 5% of national energy consumption. We present renewable energy technologies and energy self-sufficiency scenarios in a municipal WWTP ($30,000m^3d^{-1}$) located in Yongin, South Korea. By employing photovoltaics (PV, 135 kW), small hydropower turbine (10 kW), and thermal energy from treated effluent (25 RT: refrigeration ton) within the WWTP, a total of 142 tonne of oil equivalent (toe) of energy was estimated to be generated, accounting for $365ton\;CO_2\;yr^{-1}$ of greenhouse gas emission reduction. Core renewable technologies under consideration include 1) hybrid solar PV system consisting of fixed PV, dual-axis PV, and building integrated PV, 2) low-head small hydropower plant specifically designed for treated effluent, 3) effluent heat recovery system for heating and air conditioning. In addition to these core technologies, smart operation and management scheme will be presented for enhancing overall energy savings and distribution within the WWTP.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.2
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• pp.828-834
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• 2013

In this paper, TMC (Tracker Motion Controller) as one of the many research methods for condensing efficiency improvements can be condensed into efficient solar system configuration to improve the power generation efficiency of the castle with Concentrated solar silicon and photovoltaic systems (CPV)experiments using PV systems. Microprocessor used on the solar system, tracing the development of solar altitude and latitude of each is calculated in real time. Also accept the value from the sensor, motor control and communication with the central control system by calculating the value of the current position of the sun, there is a growing burden on the applicability. Through the way the program is appropriate for solar power systems and sensors hybrid-type algorithm was implemented in the ARM core with built-in TMC, Concentrated CPV system compared to the existing PV systems, through the implementation of the TMC in the country's power generation efficiency compared and analyzed. Sensor method using existing experimental results Concentrated solar power systems to communicate the value of GPS location tracking method hybrid solar horizons in the coordinate system of the sun's azimuth and elevation angles calculated by the program in the calculations of astronomy through experimental resultslook clear day at high solar irradiation were shown to have a large difference. Stopped after a certain period of time, the sun appears in the blind spot of the sensor, the sensor error that can occur from climate change, however, do not have a cloudy and clear day solar radiation sensor does not keep track of the position of the sun, rather than the sensor of excellence could be found. It is expected that research is constantly needed for the system with ongoing research for development of solar cell efficiency increases to reduce the production cost of power generation, high efficiency condensing type according to the change of climate with the optimal development of the ability TMC.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2002.10a
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• pp.18-25
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• 2002

The pikromycin biosynthetic system in Streptomyces venezuleae is unique for its ability to produce two groups of antibiotics that include the 12-membered ring macrolides methymycin and neomethymycin, and the 14-membered ring macrolides narbomycin and pikromycin. The metabolic pathway also contains two post polyketide-modification enzymes, a glycosyltransferase and P450 hydroxylase that have unusually broad substrate specificities. In order to explore further the substrate flexibility of these enzymes a series of hybrid polyketide synthases were constructed and their metabolic products characterized. The plasmid-based replacement of the multifunctional protein subunits of the pikromycin PKS in S. venezuelae by the corresponding subunits from heterologous modular PKSs resulted in recombinant strains that produce both 12- and 14-membered ring macrolactones with predicted structural alterations. In all cases, novel macrolactones were produced and further modified by the DesVII glycosyltransferase and PikC hydroxylase leading to biologically active macrolide structures. These results demonstrate that hybrid PKSs in S. venezuelae can produce a multiplicity of new macrolactones that are modified further by the highly flexible DesVII glycosyltransferase and PikC hydroxylase tailoring enzymes. This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products. The polyketide backbone of rifamycin B is assembled through successive condensation and ${\beta}$-carbonyl processing of the extender units by the modular rifamycin PKS. The eighth module, in the RifD protein, contains nonfunctional DH domain and functional KR domain, which specify the reduction of the ${\beta}$-carbonyl group resulting in the C-21 bydroxyl of rifamycin B. A four amino acid substitution and one amino acid deletion were introduced in the putative NADPH binding motif in the proposed KR domain encoded by rifD. This strategy of mutation was based on the amino acid sequences of the corresponding motif of the KR domain of module 3 in the RifA protein, which is believed dysfunctional, so as to introduce a minimum alteration and retain the reading frame intact, yet ensure loss of function. The resulting strain produces linear polyketides, from tetraketide to octaketide, which are also produced by a rifD disrupted mutant as a consequence of premature termination of polyketide assembly. Much of the structural diversity within the polyketide superfamily of natural products is due to the ability of PKSs to vary the reduction level of every other alternate carbon atom in the backbone. Thus, the ability to introduce heterologous reductive segments such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER) into modules that naturally lack these activities would increase the power of the combinatorial biosynthetic toolbox. The dehydratase domain of module 7 of the rifamycin PKS, which is predicted to be nonfunctional in view of the sequence of the apparent active site, was replaced with its functional homolog from module 7 of rapamycin-producing polyketide synthase. The resulting mutant strain behaved like a rifC disrupted mutant, i.e., it accumulated the heptaketide intermediate and its precursors. This result points out a major difficulty we have encountered with all the Amycolatopsis mediterranei strain containing hybrid polyketide synthases: all the engineered strains prepared so far accumulate a plethora of products derived from the polyketide chain assembly intermediates as major products instead of just analogs of rifamycin B or its ansamycin precursors.

• Journal of Plant Biotechnology
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• v.48 no.1
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• pp.34-43
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• 2021

Targeted genome editing using the CRISPR/Cas9 system is a ground-breaking technology that is being widely used to produce plants with useful traits. However, for woody plants, only a few successful attempts have been reported. These successes have used Agrobacterium-mediated transformation, which has been reported to be very efficient at producing genetically modified trees. Nonetheless, there are unresolved problems with plasmid sequences that remain in the plant genome. In this study, we demonstrated a DNA-free genome editing technique in which purified CRISPR/Cas9 ribonucleoproteins (RNPs) are delivered directly to the protoplasts of a hybrid poplar (Populus alba × Populus glandulosa). We designed three single-guide RNAs (sgRNAs) to target the stress-associated protein 1 gene (PagSAP1) in the hybrid poplar. Deep sequencing results showed that pre-assembled RNPs had a more efficient target mutagenesis insertion and deletion (indel) frequency than did non-assembled RNPs. Moreover, the RNP of sgRNA3 had a significantly higher editing efficacy than those of sgRNA1 and sgRNA2. Our results suggest that the CRISPR/Cas9 ribonucleoprotein-mediated transfection approach is useful for the production of transgene-free genome-edited tree plants.

• The KIPS Transactions:PartB
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• v.9B no.3
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• pp.263-270
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• 2002

Most conventional systems of fire fighting control in a ship have been based on rule-based system in which expert knowledges are expressed with production rules. Renewing and adding of rules is needed continuously for the improvement of the system capability in an already build-up system and such adding and renewing procedures could hinder users from fluent utilization of a system. The author proposes an advanced fire fighting control intelligent system (A-FFIS) using rule-based and carte-based reasoning by clustering to implement conventional hybrid system (H-FFIS). Compared with H-FFIS, new approach with A-FFIS shows that the system proposed here improves fire detection rate and reduces fire detection time.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.13-13
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• 2014

Two kinds of mushrooms, Gumji (金芝; Ganoderma) and Soji, were described in old book of Samguksagi (History of the three kingdoms; B.C 57~A.D 668; written by Bu Sik Kim in 1145) in Korea-dynasty. Many kinds of mushrooms were also described in more than 17 kinds of old books during Chosun-dynasty (1392~1910) in Korea. Nowadays, mushroom cultivation has been increased through out the world last decade years. Production of mushrooms has also been increased 10-20% and many varieties have been cultivated. Similar trends were also observed in Korea. Approximately two hundred commercial strains of 37 species in mushrooms were developed and distributed to cultivators. Somatic hybrid variety of oyster mushroom 'Wonhyeong-neutari' were developed by protoplast fusion, and distributed to grower in 1989. The fruiting body yield index of somatic hybrids of Pleurotus ranged between 27 and 155 compared to parental values of 100 and 138. In addition, more diverse mushroom varieties such as Phellinus baumi, Auricularia spp., Pleurotus ferulae, Hericium erinaceus, Hypsizigus marmoreus, Grifola frondosa, Agrocybe aegerita and Pleurotus cornucopiae have been attempted to cultivate in small scale cultivation. Production of mushrooms as food was 190,111 metric tons valued at 800 billion Korean Won (one trillion won if include mushroom factory products; 1dollar = 1,040 Won) in 2011. Major cultivated species are Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutipes, Lentinula edodes, Agaricus bisporus, and Ganoderma lucidum, which cover 90% of total production. Since mushroom export was initiated from 1960 to 1980, the export and import of mushrooms have been increased in Korea. Technology developed for liquid spawn production and automatic cultivation systems lead to the reduction of the production cost resulting in the increasement of mushroom export. However some species were imported because of high production cost for these mushrooms requiring the effective cultivation methods. Developing of effective post-harvest system will be also directly related to mushroom export. In academic area, RDA scientists have been conducting mushroom genome projects. One of the main results is the whole genome sequencing of Flammulina velutipes for molecular breeding. An electrophoretic karyotype of of F. velutipes was obtained using CHEF with 7 chromosomes, with a total genome size of approximately 26.7 Mb. The mususcript of the genome of F. velutipes was published in PLOS ONE this year. For medicinal mushrooms, we have been conducting the genome research on Cordyceps and its related species for developing functional foods using this mushroom. In 2013, Korea Food and Drug Administraion (KFDA) approved Cordyceps mushroom for its value as an immune booster.

• Journal of Korean Society for Atmospheric Environment
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• v.24 no.6
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• pp.662-673
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• 2008

Two hybrid receptor models, potential source contribution function (PSCF) and concentration weighted tracjectory (CWT), were compared for locating $PM_{2.5}$ sources contributing to the atmospheric $PM_{2.5}$ concentrations in Seoul. The source contribution estimates by chemical receptor model (CMB) receptor model were used to identify better source areas, Among the sources, soil, agricultural burning, marine aerosol, coal-fired power plant and Chinese aerosol were only considered for the study because these sources were more likely to be associated with the long-range transport of air pollutant. Both methods are based on combining chemical data with calculated air parcel backward trajectories. However, the PSCF analyses were performed with trajectories above the $75^{th}$ percentile criterion values, while the CWT analyses used all trajectories. This difference resulted in locating of different sources, which might be helpful to interpret locating of $PM_{2.5}$ sources, High possible source areas in source contribution of soil and agricultural burning contributing to the Seoul $PM_{2.5}$ were inland areas of Heibei and Shandong provinces (highest density areas of agricultural production and population) in China. The "Chinese aerosol" was used as a representative source for the $PM_{2.5}$ originated from urban area in China. High possible source areas for the aerosol were the cities in China where are relatively close to the receptor. This result suggests that Chinese aerosol is likely to be a useful tool in studies on source apportionment and identification in Korea.

• The Transactions of the Korea Information Processing Society
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• v.6 no.4
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• pp.959-968
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• 1999

several papers have reported that parallel heuristics or hybrid approaches combining several heuristics can get better results. However, the parallelization and hybridization of any search methods on the single CPU type computer need enormous computation time. that case, we need more elegant combination method. For this purpose, we propose Fast Model of Parallel Heuristics(FMPH). FMPH is based on the island model of parallel genetic algorithms and takes local search to the elite solution obtained form each island(sub group). In this paper we introduce how can we adapt FMPH to the job-shop scheduling problem notorious as the most difficult NP-hard problem and report the excellent results of several famous benchmark problems.

• Tunnel and Underground Space
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• v.27 no.6
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• pp.406-421
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• 2017

This study describes a precise numerical analysis process by adopting the real image of mine openings obtained by LIDAR, which can produce a point cloud data by measuring the target surface numerically. Research area is a section of underground limestone mine which is used hybrid room-and-pillar method for improving the production rate. From the application of LIDAR to this section several results were deduced, that is, the central axis of upper and lower vertical safety pillars is distorted to the direction of NW and the section area of lower vertical safety pillar is $34m^2$ smaller than the designed area of $100m^2$. The results of precise measurement in geometrical shape of mine openings and precise simulation in numerical analysis confirms that LIDAR techniques can be suggested as a valuable tool for stability analysis in underground mine by configuring the mine opening shape.