• 대한의생명과학회지
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• 제9권4호
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• pp.231-240
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• 2003

Deciphering the molecular interactions of proteins forming Z-lines is pivotal for understanding the regulation of myofibril assembly, sarcomeric organization, and mechanical properties of striated muscle. The purpose of this study is to searched for potential novel ligands of the Z-line portion of nebulin. In this study interacting proteins with intra-Z-line region of nebulin were screened using a yeast two-hybrid approach. The interaction was conformed by GST pull-down assay. We identified 269 residues within villin/gelsolin homology domain of supervillin that intreacts with the serine rich region of nebulin. The specific interactions of nebulin and supervillin were confirmed in vitro by GST pull-down experiments. Our data suggest that supervillin attaches directly to the Z-line through its interaction with the serine rich domain of nebulin in skeletal muscles. This interaction may link myofibrillar Z-discs to the membrane-associated complexes, costameres.

• Industrial Engineering and Management Systems
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• 제16권1호
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• pp.129-140
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• 2017

This research was conducted in order to solve the problem of selecting an operational strategy for projects in project-based organizations by designing a fuzzy expert system. In the current research, we first determined the contributing parameters in operational strategy of project-based organizations based on existing research literature and experts' opinion. Next, we divided them into two groups of model inputs and outputs and the rules governing them were determined by referring to research literature and educational instances. In order to integrate rules, the revised Ternary Grid (revised TG) and expert opinions were applied according to a hybrid algorithm. The Ultimate rules were provided in Fuzzy Inference System format (FIS). In this FIS, proper manufacturing decisions are recommended to the user based on selected competitive priority and also project properties. This paper is the first study in which rules and relations governing the parameters contributing operational strategy in project-based organizations are acquired in a guided integrated process and in the shape of an expert system. Using the decision support system presented in this research, managers of project-based organizations can easily become informed of proper manufacturing decisions in proportion with selected competitive priority and project properties; and also be ensured that theoretical background and past experiences are considered.

• 한국실내디자인학회논문집
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• 제19권5호
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• pp.83-94
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• 2010

Conventionally, ornament has developed around linear thinking based on Euclidean geometry, and been explained as simple and lucid natural Euclidean geometrical phenomena. The modular arrangement with vertical, horizontal and diagonal grids has been an organizing principle of classical ornament, but in digital era ornament is found not to be explained only with the principle of traditional arrangement due to the seemingly irregular complex forms. In that sense, this study presents the concept of digital ornament and examined the backgrounds of ornament in digital age, that are complex system and non-Euclidean geometry. Accordingly, the present study takes an approach by dividing new formal types of ornament into algorithmic form, hybrid form and dynamic form to find out a principle of pattern organization. Lately, architects who actively use computer for their architectural designs take the algorithmic strategies in nature and create various and complex patterns by simple rules. The patterns are not the repetition of the same, but the production of singularities. In addition, hybrid form by morphing shows a topologically flexible evolutionary transformation, and is used to create in-between transitional shapes from the source to target. Finally, the patterns by the interaction between the system components which are corresponded to the embedded forces emerge from dynamic simulation of the natural environment. Rather than objects itself, focus is given to the process of generating forms, and the ornamental patterns as the revelation of such implicit order provide not just the formal beauty but also spatial pathways for lights and air, maximizing the effects of lights.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2011년도 한국컴퓨터종합학술대회논문집 Vol.38 No.1(A)
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• pp.112-115
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• 2011

Healthcare is one of the biggest industries that consume IT Infrastructure as a necessity. A reliable reach to secure private information is the essential goal. Most of the healthcare organizations either have their own custom solutions developed or third part CRM software tailored to their needs. This culture satisfies the usability internal to the organization but lacks the ability to provide public access to the users or even to take a step ahead and collaborate with like minded institutes for research and potential growth. Emergence of cloud computing has provided us the opportunity to take a step ahead and build applications available to users on a secure platform, with ability to scale the resources depending on the requirements. The effective use of hybrid and community cloud can create rock solid foundations for healthcare IT Infrastructure. These cloud deployment models not only caters the present needs of healthcare IT Infrastructure but also opens the possibilities to build applications and provide secure and reliable access beyond the limits of organizational boundaries.

• 동남아시아연구
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• 제23권1호
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• pp.89-126
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• 2013

The Red shirts came to attract attention of the international community during April to May in 2010 by successfully organizing explosive popular demonstrations. The momentum was the military coup on September 9, 2006. The Red color was chosen amid movements against the new constitution instituted under the military junta. In discourse struggles, the Red shirts compared their resistance against the Democratic Party government lead by Abhisit Vejjajiva to that of phrai (commoner or serfs) against ammart (aristocrats or bureaucrats) under the pre-modern reign of sakdina. The Red shirts strongly accused Prem Tinsulanonda, the chief of the Privy Council, of being a mastermind of 2006 military coup, who symbolically represents the cohesion between the palace and the military. It has constituted an unprecedented defiance towards national taboo where the trinity of Nation, Religion, and King has been consecrated. The objective of this article is to review the Red Shirts' ideology, organizations and activities in terms of the modernized phrai's struggles for expanding counter-hegemony. While Antonio Gramsci focused on why socialist revolution had failed to materialize in capitalist Western Europe, I pay attention to why political liberalism has failed to wash away pre-modernity and take root in capitalist Thailand, applying the Gramscian concept of hegemony by contrasting 'hybrid ammart' with 'modernized phrai'.

• 컴퓨터교육학회논문지
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• 제3권2호
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• pp.67-75
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• 2000

인터넷의 출현으로 많은 교육자들은 인터넷을 교육용 도구로 사용하고 있고, 학교내의 많은 업무가 전산화되면서 인터넷을 활용한 사례가 증가하고 있다. 하지만, 인터넷의 개방성으로 인해 학교망은 외부망에 그대로 노출되어 있기 때문에 교육자료의 파손이나 온라인 성적처리 시 데이터 변경 등의 문제점을 가지고 있다. 외부망으로부터 보호에 대한 요구가 증가하고 있으나, 아직 방화벽을 구축하고 있는 중 고등학교가 전무하며 설치 및 운영 시에도 어려움이 따른다. 본 논문에서는 이와 같은 문제점을 해결하기 위해 학교망을 위한 혼합방화벽을 용이하게 구축 관리할 수 있는 방안을 제시하고, 외부로부터 접근제어를 위해 접근제어 규칙을 용이하게 기술할 수 있고 로그 분석, 실시간 네트워크 트래픽 감시, 날짜별 트래픽 통계 정보 등을 제공하는 그래픽적인 사용자 인터페이스를 구현한다.

• 정보처리학회논문지A
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• 제12A권2호
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• pp.171-180
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• 2005

분산 시스템에서 가용성을 높이고 전체 시스템의 성능을 향상시키기 위해 데이터는 여러 노드에 중복하여 저장된다. 여기서는 전역적 접근제어를 위해서 읽기/쓰기 동작을 수행하는데 필요한 노드의 집합을 정의하는 Quorum 프로토콜이 존재한다. Quorum 프로토콜을 사용하는 대표적인 복제 프로토콜인 Tree Quorum 프로토콜은 트리의 높이가 증가할수록 노드의 수가 기하급수적으로 증가하고, Grid 프로토콜은 노드에 장애가 발생하지 않아도 언제나 같은 읽기/쓰기 비용을 갖는다는 단점을 갖고 있다. 따라서, 본 논문에서는 기존 프로토콜의 장점을 가지면서 단점을 해결할 수 있는 새로운 하이브리드 프로토콜을 제안한다. 제안된 하이브리드 프로토콜은 전체적으로는 트리 구조를 가지면서 각 레벨에서는 그리드의 열과 같은 구조를 가짐으로써 노드에 장애가 없을 때에는 Tree Quorum 프로토콜과 같이 적은 동작 비용을 요구하며, 노드에 장애가 존재할 경우에도 기존 프로토콜에 비해 상대적으로 적은 동자 비용과 높은 가용성을 보인다. 그러므로 높은 데이터 가용성이 요구되는 서바이벌 스토리지 시스템에 효율적으로 적용 가능하다. 본 논문에서는 수학적 모델링을 통하여 제안된 프로토콜의 비용과 가용성을 평가하고, 시뮬레이션을 통해 응답시간과 처리율을 기존의 Tree quorum프로토콜과 비교한다.

• 한국육종학회지
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• 제41권3호
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• pp.194-204
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• 2009

Cytoplasmic male sterility (CMS) and fertility restoration have been utilized as valuable tools for $F_1$-hybrid seed production in many crops despite laborious breeding processes. Molecular markers for the selection of CMS-related genes help reduce the expenses and breeding times. A previously reported genomic region containing the Ppr-B gene, which is responsible for restoration of fertility and corresponds to the Rfo locus, was used to develop gene-based or so-called "functional" markers for allelic selection of the restorer-of-fertility gene (Rfo) in $F_1$-hybrid breeding of radish (Raphanus sativus L.) Polymorphic sequences among Rfo alleles of diverse breeding lines of radish were examined by sequencing the Ppr-B alleles. However, presence of Ppr-B homolog, designated as Ppr-D, interferes on specific PCR amplification of Ppr-B in certain breeding lines. The organization of Ppr-D, resolved by genome walking, revealed extended homology with Ppr-B even in the promoter region. Interestingly, PCR amplification of Ppr-D was repeatedly unsuccessful in certain breeding lines implying the lack of Ppr-D in these radishes. Ppr-B could only be successfully amplified for analysis through designing primers based on the sequences unique to Ppr-B that exclude interference from Ppr-D gene. Four variants of Rfo alleles were identified from 20 breeding lines. A combination of three molecular markers was developed in order to genotype the Rfo locus based on polymorphisms among four different variants. These markers will be useful in facilitating $F_1$-hybrid cultivar development in radish.

• 간호행정학회지
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• 제10권1호
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• pp.49-62
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• 2004

purpose: The study was done to analyze the concept of moral distress in nurses. Method: The Hybrid Model was used in this study. For the theoretical phase, nursing and other literature were reviewed to analyze attributes and develop a working definition of the concept, moral distress in nurses. For the fieldwork phase, In-depth interviews were conducted with six nurses. Results: Four higher level concepts of moral distress in nurses were found; situational, cognitive, behavioral, and emotional, and 8 subscales, negative medical behavior, negative nursing behavior, excessive economic load, irrational organization administration, internal restraints, external restraints, negative behavioral responses, and negative emotional responses. Conclusion: it is necessary to develop programs of arbitration that will lessen the moral distress in nurses working in clinical practice.

• Journal of Microbiology
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• 제44권3호
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• pp.311-319
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• 2006

Bud6p is a component of a polarisome that controls cell polarity in Saccharomyces cerevisiae. In this study, we investigated the role of the Candide albicans Bud6 protein (CaBud6p) in cell polarity and hyphal development. CaBud6p, which consists of 703 amino acids, had 37% amino-acid sequence identity with the Bud6 protein of S. cerevisiae. The homozygous knock-out of CaBUD6 resulted in several abnormal phenotypes, such as a round and enlarged cells, widened bud necks, and a random budding pattern. In hypha-inducing media, the mutant cells had markedly swollen tips and a reduced ability to switch from yeast to hypha. In addition, a yeast two-Hybrid analysis showed a physical interaction between CaBud6p and CaAct1p, which suggests that CaBud6p may be involved in actin cable organization, like Bud6p in S. cerevisiae. Taken together, these results indicate that CaBud6 plays an important role in the polarized growth of C. albicans.