• Journal of the Korean Society for information Management
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• v.25 no.4
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• pp.283-308
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• 2008

In this study, we testify that network structural attributes of a citation network can explain other aspects of journal citation behaviors and the importances of journals. And we also testify various citation impact indicators of journals including JIF and h-index to verify the difference among them especially focused on their ability to explain an institution's local features of citation behaviors. An institutional citation network is derived using the articles published in 2006-2007 by biotechnology faculties of Y University. And various journal citation impact indicators including JIF, SJR, h-index, EigenFactor, JII are gathered from different service sites such as Web of Science, SCImago, EigenFactor.com, Journal-Ranking.com. As a results, we can explain the institution's 5 research domains with inter-citation network. And we find that the co-citation network structural features can show explanations on the patterns of institutional journal citation behavior different from the simple cited frequency of the institution or patterns based on general citation indicators. Also We find that journal ranks with various citation indicators have differences and it implies that total-based indices, average-based indices, and hybrid index(h-index) explain different aspects of journal citation pattern. We also reveal that the coverage of citation DB doesn't be a matter in the journal ranking. Analyzing the citation networks derived from an institution's research outputs can be a useful and effective method in developing several library services.

• The Transactions of the Korea Information Processing Society
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• v.4 no.6
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• pp.1519-1530
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• 1997

Object-oriented software, reusability enables to specialize the software techniques by leading the user friendly thought and meets the user's need only concentrating on convenience of development environment. Reuse effect can be maximized through retrieval system. In this paper, we development the MT-Views(Multiple-Template Views) to realize the reuse effect as integrated reuse system including retrieval, understanding and rebuilding tools based on class library. We used the hybrid retrieval model for easy and efficient retrieval of similar software components. With using these tools, users can retrieve the reusable components and reuse the components for constructing therir own applications. Also, we can acquire many informations about retrieved components, so can rebuild components conforming our original intend.

• Journal of Korean Society of Forest Science
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• v.94 no.4 s.161
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• pp.236-242
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• 2005

Phytochrome B (PhyB) gene, which is a photoreceptor that controls plant growth under various light conditions, was cloned from Chinese hybrid poplar 'Soohang 1'. Nucleotide sequence and deduced amino acid sequences PhyB cDNA of 'Soohang' is consisted with 3,456 nucleotides and 1,156 amino acids. The cloned PhyB fragment showed 98% homology of amino acid sequences with Populus balsamifera PhyB1. According to Northern blot analysis. PhyB was up-regulated by light, while PhyB transcript was not detected under dark condition. According to this study, the cloned PhyB is induced by light and functions as photoreceptor.

• Journal of Microbiology
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• v.34 no.4
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• pp.327-333
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• 1996

Yeast, like many other microbes, encounters large variations in ambient pH in their natural environments. Microorganisms capable of growing over a wide pH range require a versatile, efficient pH homeostatic mechanism protecting intracellular processes against extremes of pH. In several organisms, fusions to the bacterial lacZ gene have been extremely useful for the identification of genes expressed at different time during the life cycle or under different growth conditions. In this study, using the lacZ gene screening system, we surveyed a large number of yeast strains with lacZ insertion to identify genes regulated by pH. A yeast genomic library was constructed and inserted with lacZ by a shuttle mutagenesis procedure. The yeast transformants were individually picked up with a toothpick, replica-plated, and grown in alkaline pH medium. Among the 35,000 colonies screened, 10 candidate strains were identified initially by the $\beta$-gal assay. We finally confirmed two yeast strains carrying the genes whose expression are strictly dependent on pH of growth medium. One of the fusions showing a 10-fold induction in expression level in response to alkali pH was selected and further characterized. The pH-regulated gene was cloned by inverse PCR and a partial sequence of the gene was determined. Identification and characterization of the gene is currently under investigation.

• Journal of Information Management
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• v.26 no.2
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• pp.61-79
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• 1995

The purpose of this paper is to investigate search efficiency of controlled vocabulary versus uncontrolled vocabulary subject access in online catalog systems. The question of the effectiveness of controlled versus uncontrolled vocabulary in information retrieval has been raised in many literatures. A debate continues in the Library and Information Science Professions over the relative merit, appropriateness, and efficiency of uncontrolled vocabulary subject access in online catalog systems. Actually users used to combine uncontrolled vocabulary subject searching with controlled vocabulary subject searching. But the success of user's subject search depends on his choice of search terms. Also the technical developments that facilitate cooperation among information services in general make it increasingly possible for such cooperation to take place on an international level. In this study, several common types of vocabularies on online catalog systems are described and compared, especially usages of vocabularies in multilingual environment are analyzed.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.346-353
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• 2005

A 50-kb chromosome DNA region was isolated from Streptomyces kanamyceticus by screening the fosmid genomic library, using the 16S rRNA methylase gene (kmr) as a probe. Sequence analysis of this region revealed 42 putative open reading frames (ORFs), which included biosynthetic genes such as genes responsible for 2-deoxystreptamine (2­DOS) biosynthesis as well as genes for resistance and regulatory function. Also, the kanamycin acetyltransferase gene (kac) was characterized by in vitro enzyme assay, which conferred E. coli BL21 (DE3) with 10, 50, and 80-times higher resistance to kanamycin A, tobramycin, and amikacin, respectively, than the control strain had, thus strongly indicating that the isolated gene cluster is very likely involved in kanamycin biosynthesis. This work provides a solid basis for further elucidation of the kanamycin biosynthesis pathway as well as the productivity improvement and construction of new hybrid antibiotics.

• Journal of Microbiology
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• v.37 no.1
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• pp.35-40
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• 1999

Sporulation-specific glucoamylase (SGA) gene was isolated from genomic library of Saccharomyces diastaticus 5114-9A by using glucoamylase non-producing mutant of S. diastaticus as a recipient. When the glucoamylase activities of culture supernatant, periplasmic, and intracellular fraction of cells transformed with hybrid plasmid containing SGA gene were measured, the highest activity was detected in culture supernatant. SGA produced by transformant and extracellular glucoamylase produced by S. diastaticus 5114-9A differed in enzyme characteristics such as optimum temperature, thermostability, and resistance to SDS and urea. But the characteristics of SGA produced by sporulating yeast cells and vegetatively growing transformants were identical.

• Journal of Microbiology
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• v.41 no.4
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• pp.295-299
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• 2003

Adenovirus (Ad) precursor to the terminal protein (pTP) plays an essential roles in the viral DNA replication. Ad pTP serves as a primer for the synthesis of a new DNA strand during the initiation step of replication. In addition, Ad pTP forms organized spherical replication foci on the nuclear matrix (NM) and anchors the viral genome to the NM. Here we identified the interferon inducible gene product 1-8D (Inid) as a pTP binding protein by using a two-hybrid screen of a HeLa cDNA library. Of the clones obtained in this assay, nine were identical to the Inid, a 13-kDa polypeptide that shares homology with genes 1-8U and Leu-13/9-27, most of which have little known functions. The entire open reading frame (ORF) of Inid was cloned into the tetracycline inducible expression vector in order to determine the biological functions related with adenoviral infection. When Inid was introduced to the cells along with adenoviruses, fifty to sixty percent of Ad-infected cells expressing Inid had rounded morphology, which was suggestive of apoptosis. Results from the terminal deoxynucleotidyl transferase (TdT) and DNA fragmentation assays confirmed that Inid induces apoptosis in Ad-infected or in uninfected cells. The Inid binding to pTP may target the cell for apoptotic destruction as a host defense mechanism against the viral infection.

• Journal of Information Science Theory and Practice
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• v.8 no.1
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• pp.6-19
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• 2020

When information professionals deal with other disciplines in the course of digital humanities projects, they often assume that they are dealing with 'needful users' who have an 'information gap' to fill. This paper argues that the traditional view that information/knowledge is transferred from an information specialist donor to a domain specialist receiver is no longer appropriate in the digital humanities context, where the gap-and-search (or gap-and-filler) approach to information has given way to more direct, explorative engagement with information. The paper asks whether information science and the practising profession are ready for this paradigm shift and examines information science conservatism in two common collaboration scenarios, library support and digital development. It is shown that information science theory still assumes a traditional donor role in both scenarios. How information scientists deal with conservatism in practice is discussed in the example of the Prior project, in which the information science team exerted an ambiguous, hybrid approach with both conservative and non-conservative elements. Finally, two rather hypothetical answers are offered to the question of how information professionals should approach scholarly collaboration in the digital humanities context, where users have ceased to be supplicants. From a purely pragmatic perspective, information scientists need to shift their focus from information needs to research practices and the implications of these practices for digital information systems. More fundamentally, the emergence of digital humanities challenges information professionals to transform information systems designed for searching into digital objects that can be explored more freely by the digital humanities community.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.913-916
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• 2009

This paper describes a hardware design of hash processor which implements HAS-160 algorithm adopted as a Korean standard. To achieve a high-speed operation with small-area, the arithmetic operation is implemented using a hybrid structure of 5:3 and 3:2 carry-save adders and a carry-select adder. The HAS-160 processor synthesized with $0.35-{\mu}m$ CMOS cell library has 17,600 gates. It computes a 160-bit hash code from a message block of 512 bits in 82 clock cycles, and has 312 Mbps throughput at 50 MHz@3.3-V clock frequency.