• 대한의생명과학회지
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• 제11권1호
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• pp.51-56
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• 2005

The detection of high-risk human papilloma virus (HPV) allows us to predict the presence and future development of cervical intraepitheliallesion. In this study, we compared Hybrid Capture II and DNA chip methods for detection of HPV in cervical swab samples. And we evaluated the clinical efficacy and diagnostic performance of HPV DNA chip and Hybrid Capture II for detecting HPV in cervical neoplastic lesions. Seventy four patients were classified into three groups according to their histologic diagnosis: Group I (nonspecific chronic cervicitis), Group II (low-grade squamous intraepithelial lesion (SIL); koilocytosis, and mild dysplasia), and Group III (high-grade SIL;, moderate, severe dysplasia and in situ carcinoma). Cytologic diagnosis were based on the Bethesda System. Hybrid Capture II and DNA chip methods were performed to detect HPV. In 41 of the 74 cervical samples $(55.4\%)$, HPV DNAs were detected by Hybrid Capture II. In Group III, HPV-positive cases were detected in 15 $(20.3\%)$ of 74 patients by Hybrid Capture II. 25 patients with ASCUS cytology were histopathologically examined: 9 cases $(36\%)$ were Group II. In 18 patients with low-grade SIL cytology, 13 cases $(72.2\%)$ were Group II and 3 cases $(16.7\%)$ were Group III. 12 cases $(92.3\%)$ were Group ill of 13 patients with high-grade SIL cytology. The sensitivity of each test was $82\%$ in Hybrid Capture II and $53.9\%$ in DNA chip test. And the specificity was $74.3\%,\;85.7\%$ in Hybrid Capture II and DNA chip. In conclusion, Hybrid Capture II test is more sensitive than DNA chip in detecting women with cervical neoplastic lesions. Especially, in diagnosing of ASCUS, Hybrid Capture II test is more sensitive. Therefore, Hybrid Capture II test for cancer-associated HPV DNA is a viable option in the management of women with ASCUS.

• 한국콘텐츠학회논문지
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• 제13권8호
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• pp.308-313
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• 2013

세포선별검사에서 ASCUS로 진단된 검체 중 F-UP이 진행되지 않은 검체 179건을 대상으로 HPV high risk factor, low risk factor를 확인하기 위해서 Hybrid Capture II Test를 하였다. 시험 결과 high risk factor 양성 및 low risk factor 양성은 전체 179 cases 중 26 cases로 나타났으며, 전체 검체 중 약 15%에 해당되었다. 또한 전체 15%의 양성검체 중 high risk factor 양성은 96%에 해당되는 것을 확인하였다. 결론적으로 효과적인 검진 체계를 갖춘 나라에서도 자궁경부암의 예방이 완전히 해결되지 않고 있는 문제점이 있는 여건을 고려해 볼 때 추적 관찰이 용이하지 않은 경우를 대비해 자궁경부암으로 진행될 수 있는 precancerous lesion의 원인 인자인 HPV 존재 유무를 세포선별검사와 HPV Hybrid Capture II Test를 동시에 시행하는 것이 효과적이라고 사료된다.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2012년도 춘계 종합학술대회 논문집
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• pp.175-176
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• 2012

세포선별검사에서 ASCUS로 진단된 검체 중 F-UP이 진행되지 않은 검체 179건을 대상으로 HPV High risk factor, Low risk factor를 확인하기 위해서 Hybrid Capture II Test를 하였다. 결과 High risk factor 양성 및 Low risk factor 양성은 전체 179 cases 중 25 cases로 나타났으며, 전체 검체 중 약 15%에 해당되었다. 또한 전체 15%의 양성검체 중 High risk factor 양성은 87%에 해당되는 것을 확인하였다. 결론적으로 효과적인 검진 체계를 갖춘 나라에서도 자궁경부암의 예방이 완전히 해결되지 않고 있는 문제점이 있는 여건을 고려해 볼 때 추적 관찰이 용이하지 않은 경우를 대비해 자궁경부암으로 진행될 수 있는 precancerous lesion의 원인 인자인 HPV 존재 유무를 세포선별검사와 HPV Hybrid Capture II Test를 동시에 시행하는 것이 효과적이라고 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4465-4466
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• 2014

HPV type-specific detection may promote cervical screening program and vaccination development worldwide. We conduct a study comparing HPV Hybrid capture II (HC II) Test and Hybribio GenoArray test, a newly developed HPV type-specific assay, in patients with cervical epithelial neoplasm. Results showed a good concordance in cervical HPV detection between two tests (kappa value 0.80, p<0.05, McNemar test). Our study may promote utilization of type-specific HPV detection that is helpful for cervical cancer screening and vaccination.

• 대한세포병리학회지
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• 제19권2호
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• pp.119-125
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• 2008

This study was performed to compare the efficacy between a DNA chip method and a Hybrid-Capture II assay (HC-II) for detecting human papillomavirus in patients with intraepithelial lesions of the uterine cervix. From May, 2005, to June, 2006, 192 patients with abnormal colposcopic findings received cervical cytology, HC-II and HPV DNA chip tests, and colposcopic biopsy or conization. We compared the results of HC-II and HPV DNA chip in conjunction with liquid based cervical cytology (LBCC) and confirmed the results of biopsy or conization. The sensitivity of the HPV DNA chip test was higher than HC-II or LBCC. The HPV DNA chip in conjunction with LBCC showed higher sensitivity than any single method and higher sensitivity than HC-II with LBCC. We confirmed that the HPV DNA chip test was more sensitive for detecting HPV in cervical lesions than HC-II, and that it would provide more useful clinical information about HPV type and its multiple infections.

• 대한세포병리학회:학술대회논문집
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• 대한세포병리학회 2008년도 제22차 춘계학술대회
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• pp.15-15
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• 2008

• 보건교육건강증진학회지
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• 제27권1호
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• pp.1-8
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• 2010

Objectives: Human papillomavirus (HPV) infection is highly associated with cervical cancer. So, the modification of the risk factors of HPV infection is essential for prevention of cervical cancer. This study was performed to evaluate the risk factors of HPV infection. Methods: HPV test of 12,337 study population conducted using Hybrid-Capture II assay(HC-II) and self-administered questionnaires were collected. The study population was people who visited hospital-based medical screening center from January to December 2007 and all were female employees or employees' partner. Results: In logistic regression analysis, smoking and alcohol drinking were significant factors, with odds ratios of 1.328 (95% CI 1.010~1.746) and 1.644 (95% CI 1.309~2.066), respectively. Nutritional supplements was also significant factor, which odds ratio was 1.161 (95% CI 1.004~1.343). Oral contraceptives was positive association with HPV infection (odds ratio 2.108; 95% CI 1.217~3.652), whereas condom was negative association (odds ratio 0.851; 95% CI 0.740~0.979). Conclusion: HPV Prevalence of 12,377 study population was 11.4%. Smoking, alcohol drinking, nutritional supplements and oral contraceptives were possible risk factors of HPV infection, and condom had possible preventive effect on HPV infection. Further prospective and comprehensive studies about HPV risk factors are required.

• 대한임상검사과학회지
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• 제47권3호
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• pp.132-139
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• 2015

최근 $p16^{INK4a}$/Ki-67 이중면역염색은 액상세포검사에서 자궁경부 이형성증과 암을 조기에 발견하기 위한 새로운 생체 표지자로 대두되고 있다. 저자들은 자궁경부질도말의 액상세포검체 총 109례에서 CINtec$^{(R)}$ PLUS kit를 사용하여 $p16^{INK4a}$/Ki-67 이중면역염색을 시행하였고, 그 결과를 액상세포검사, HPV hybrid capture II (HC II) 검사 그리고 조직학적 진단과 서로 비교하였다. $p16^{INK4a}$/Ki-67 양성 발현은 세포학적 진단에서 저등급 편평상피내병변 이상의 증례 그리고 조직학적 진단에서 자궁경부 상피내종양 1등급 이상의 증례에서 유의미하게 높았다. 액상세포검사상 비정형 편평 상피세포 소견을 보이는 46례 중, 31례 (67.4%)가 $p16^{INK4a}$과 Ki-67 양성 소견을 보였고, 이러한 양성 증례들은 조직검사에서도 대부분 자궁경부 상피내종양 1등급 이상의 병변에 해당하였다. 자궁경부 상피내종양 1등급 이상의 병변을 발견하기 위한 $p16^{INK4a}$/Ki-67 이중면역염색의 민감도는 액상세포검사보다 높은 89.0%였고, 특이도는 73.5%로 HC II 검사보다 높게 분석되었다. 따라서, $p16^{INK4a}$/Ki-67 이중면역염색 방법은 액상세포검사법의 민감도와 HC II 검사법의 특이도를 보완하기 위한 진단적 검사로 유용하다고 할 수 있다.

• 대한세포병리학회지
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• 제16권1호
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• pp.18-24
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• 2005

The objective of this study was to ascertain whether or not the high-risk human papillomavirus (HPV) test, when coupled with Papanicolaou (Pap) smears, would prove useful in the screening and management of patients in whom abnormal Pap smear results had been obtained. Concomitant high-risk HPV detection using the hybrid capture II test and colposcopy with a Pap smear were performed with 176 patients, all of whom had been screened for both cervical carcinoma and precancerous lesions. We concomitantly performed colposcopies on these patients. Upon the follow-ups, the histologic diagnoses of these patients were confirmed via either biopsy or hysterectomy. The rate of high-risk HPV detection was correlated with cytologic diagnoses and colposcopic findings. The group composed of the high-risk HPV-positive ASCUS patients exhibited a 55.7% rate of cervical intraepithelial neoplasia (CIN), a significantly higher rate than the 7.5% result obtained in the high-risk HPV-negative ASCUS group. HPV test showed high sensitivity (87%) and low specificity (62.6%) in detection of CIN and colposcopy also showed high sensitivity (88%) and low specificity (22%). Any combination of these tests improve sensitivity, but not specificity. High-risk HPV tests, when coupled with Pap smears, constituted a useful triage approach with regard to colposcopy-directed biopsies in patients in whom a cytologic diagnosis of ASCUS had been rendered.

• 대한세포병리학회지
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• 제18권1호
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• pp.20-28
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• 2007

This study was performed to compare Surepath$^{TM}$ liquid-based smear and a conventional cervicovaginal smear with reference to a histological diagnosis. A hybrid capture test (HCII) was also performed and analyzed. We collected matched cases for cervicovaginal cytology-histology: 207 cases for conventional cytology (CC) and 199 cases for liquid-based cytology (LBC). HCII was performed in 254 patients. When a cytological diagnosis of ASCUS or above (ASCUS+) is classified as positive and a histological diagnosis of LSIL+ is classified as positive, the sensitivity and specificity for LBC was 91.7% and 75.9%, respectively and the sensitivity and specificity for CC was 62.6% and 96.1%, respectively. When a cytological and histological diagnosis of LSIL+ is classified as positive, the sensitivity and specificity for LBC was 77.5 and 96.6%, respectively and the sensitivity and specificity for CC was 49.7% and 100%, respectively. When a histological diagnosis of LSIL+ is classified as positive, the sensitivity and specificity for HCII was 78.9% and 78.1%, respectively. The concordance ratio between the cytological and histological diagnosis was 80.4% (kappa=76.0) for LBC and 56.5% (kappa=55.1) for CC. LBC is more sensitive and less specific then CC, as a cytological cutoff level of ASCUS, but more sensitive and equally specific, as a cytological cutoff level LSIL or HSIL. LBC is more reliable with a high concordance ratio between the cytological and histological diagnosis.