• Journal of the Society of Cosmetic Scientists of Korea
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• v.36 no.2
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• pp.121-128
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• 2010

We investigated the effects of skin hydration and composition analysis of proteoglycan (chia seed polymer) produced from chia (Salvia hispanica) seed. The result showed that proteoglycan of chia seeds is composed of galactose (46.8 %), glucuronic acid (27.1 %), rhamnose (8.7 %), xylose (7.6 %), glucose (4.9 %), fructose (2.3 %), mannose (1.8 %), arabinose (0.9 %) and the amount of proteins contained is 31.3 mg/g with the constituent amino acid compositions (mg/g) of Asp (1.9), Glu (3.6), Ser (0.9), Gly (3.6), Thr (0.8), Arg (1.0), Ala (2.0), Tyr (0.4), Cys (4.8), Val (1.1), Phe (0.5), Ile (0.6), Leu (0.9). The molecular weight of the proteoglycan measured by GPC (Gel Permeation Chromatography) is the range of 100,000~250,000 Da and the average molecular weight is 170,000 Da. The moisturizing effects and trans-epidermal water loss (TEWL) of chia seed polymer in cosmetic products (O/W emulsion) were studied in vivo. Chia seed polymer showed good skin hydration effects when compared with sodium hyaluronate which is a common moisturizer. Taken all together, chia seed polymer should be a very useful cosmetic ingredient as a moisturizer and a protecting agent from various skin irritations.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.35-41
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• 2005

We investigated the effects of skin hydration and anti-irritation of exopolysaceharide (GF-glucan) produced from submerged culture of Grifola frondosa. The moisturizing effects and trans-epidermal water loss (TEWL) of GF-glucan in cosmetic products (O/W emulsion) were clinically studied. GF-glucan showed good skin hydration effects when compared with sodium hyaluronate which is a commonly using moisturizer. The skin protective effects of GF-glucan against sodium lauryl sulfate (SLS) were also found. Twice a day application of the CF-glucan to skin reduced local inflammation remarkably. Irritant reactions were assessed by various aspects of skin function, erythema (skin color reflectance), hydration (electrical capacitance) and TEWL. In the human skin test, 5-day treatment of the GF-glucan was found to reduce SLS-induced skin erythema and Improve bier regeneration. Taken all together, GF-glucan should be a very useful cosmetic ingredient, as a moisturizer, and a protecting agent for various skin irritations.

• Applied Chemistry for Engineering
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• v.33 no.1
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• pp.83-89
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• 2022

Since the pandemic of COVID-19, active investigation to develop immunity to infectious disease by delivering nucleic acids has been proceeded. Particularly, many studies have been conducted on non-viral vector as several vital side-effects which were found on nucleic acid delivery system using viral vectors. In this study, we have developed plasmid DNA (pDNA) loaded-hyaluronic acid derivative (HA) coated-polyethyleneimine (PEI) based polyplex for enhanced nucleic acid delivery efficiency. We have optimized the ratio of pDNA : PEI : HA by measuring size and protein transcription efficiency. The final product, polyplex-HA, was characterized through measuring size, zeta-potential and TEM image. Intracellular uptake and protein transcription efficiency were compared to commercially available transfection reagent, lipofectamine, through fluorescence image and flow cytometry. In conclusion, polyplex-HA presents a novel gene delivery system for efficient and stable protein transcription since it is available for delivering various genetic materials and has less immunoreactivity.