• Title/Summary/Keyword: Hwang woo-suk

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Paternity test in dogs by microsatellite allele analysis (Microsatellite 대립유전자 분석을 통한 개에서의 친자감별)

  • Chae, Young-jin;Kim, Dong-keon;Kim, Hana;Lee, Moon-han;Hwang, Woo-suk;Lee, Byoung-chun;Youn, Hwa-young;Lee, Hang
    • Korean Journal of Veterinary Research
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    • v.39 no.1
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    • pp.213-219
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    • 1999
  • Microsatellite allele analysis has been used for individual identification and paternity test. In the present study, the biological father of three puppies was determined by using microsatellite allele amplification analysis. The mother bitch of the litter was a Poongsan dog. The three stud dogs that could have inseminated the bitch, by being in the same residence, were a white Poosan dog, a mixed breed, and a white Jindo dog. DNA was obtained from all the relevant dogs by buccal swabbing. Four loci of tetranucleotide repeat microsatellite were PCR-amplified, and analyzed by polyacrylamide gel electrophoresis and silver staining. The results of genotyping unambigously assigned the Poongsan dog as the biological father. There was no evidence of superfecundation. Therefore, the present study demonstrated the usefulness of microsatellite allele analysis as a simple, efficient method of paternity test in dogs.

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Influence of Curing and Heating on Formation of N-Nitrosamines from Biogenic Amines in Food Model System using Korean Traditional Fermented Fish Product

  • Mah, Jae-Hyung;Yoon, Mi-Young;Cha, Gyu-Suk;Byun, Myung-Woo;Hwang, Han-Joon
    • Food Science and Biotechnology
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    • v.14 no.1
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    • pp.168-170
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    • 2005
  • The myeolchi-jeot samples were divided into different groups with or without the supplementation with biogenic amines. Subsequently, the samples were placed in an oven at $80^{\circ}C$ for 1 hr to allow the chemical reaction to proceed, and then were analyzed for N-nitrosamine contents using GC-TEA. N-nitrosamine was not detected in any of the myeolchi-jeot samples which had been treated with/without sodium nitrite. On the other hand, the yield of N-nitrosopyrrolidine from 1,000 mg/kg of putrescine and spermidine in the myeolchi-jeot samples (treated with 5 mg/kg of sodium nitrite) was 0.002 and 0.014%, respectively. N-nitrosamine was not produced from any other biogenic amines like, histamine, tyramine, cadaverine and spermine. In addition, curing and heating were the factors which influenced the formation of N-nitrosamine during the nitrosation of biogenic polyamines. For the formation of N-nitrosamine in the food systems, treatment with sodium nitrite and heating at appropriate temperature along with the satisfied supplementation of biogenic polyamines are required.

Fabrication and Characterization of a Wrist Wearable Cuffless Pulsimeter by Using the Hall Effect Device

  • Lee, Sang-Suk;Choi, Jong-Gu;Son, Il-Ho;Kim, Keun-Ho;Nam, Dong-Hyun;Hong, You-Sik;Lee, Woo-Beom;Hwang, Do-Guwn;Rhee, Jang-Roh
    • Journal of Magnetics
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    • v.16 no.4
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    • pp.449-452
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    • 2011
  • A wrist wearable cuffless pulsimeter with a portable and small size apparatus using Hall effect is fabricated. The analysis of the pulse wave measured by the testing product of pulsimeter is done to measure the pulse rate and blood pressure. The blood pressure obtained by the puslimeter is compared with the practical values measured by electronic or mercury liquid blood pressure meters. The detail analysis of a pulse wave measured by a wrist wearable cuffless pulsimeter detecting the changes of the magnetic field can be used to develop a new diagnostic algorithm of blood pressure applying for oriental medical apparatus.

The Effect of Particle Size Distribution on the Physical and Optical Properties of Cenosphere (세노스피어(Cenosphere)의 입도 분포에 따른 물리적 특성 및 광학적 특성 평가)

  • Lee, Won-Jun;Hwang, Hae-Jin;Han, Kyu-Sung;Hwnag, Kwang-Taek;Cho, Woo-Suk;Kim, Jin-Ho
    • Korean Journal of Materials Research
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    • v.27 no.7
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    • pp.353-358
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    • 2017
  • Recycled cenosphere, which is a hollow shaped particle from fly ash, has become attractive as a building material due to its light weight and excellent heat insulation and soundproof properties. In this paper, we investigated the effect of cenosphere size on the physical and optical properties. High brightness of cenosphere as raw material is required for a wide range of ceramics applications, particularly in fields of building materials and industrial ceramic tiles. Cenospheres were sorted by particle size; the microstructure was analyzed according to the cenosphere size distribution. Cenospheres were generally composed of quartz, mullite, and amorphous phase. Colour measurement corresponding to chemical composition revealed that the contents of iron oxide and carbon in the cenospheres were the major factors determining the brightness of the cenospheres.

Improvement of Menopausal Signs by Isoflavones Derived from Sophorae fructus in Ovariectomized Female Rats and the Antioxidant Potentials in BV2 Cells

  • Joo, Seong-Soo;Kwon, Suk-Hyung;Hwang, Kwang-Woo;Lee, Do-Ik
    • Archives of Pharmacal Research
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    • v.28 no.5
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    • pp.566-572
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    • 2005
  • The aim of this study was to determine if the isoflavones from Sophorae fructus (SISO) have potential clinical benefit in hormone replacement therapy (HRT) for the treat ment of menopausal signs, such as the levels of total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and follicle stimulating hormone (FSH). An additional aim was to present the potential antioxidant effect of SISO in a microglial cell line. For the animal model, the ovaries were removed from adult rats and the indicators of menopause were measured at the pre- and post-administration time points. Although no statistically significant correlation was found, SISO tended to decrease the TC level (p=0.15) and the FSH level (p=0.36), but to increase the HDL level (p=0.303).SISO (< 5${\mu}g$/mL) also exerted antioxidant activity on BV-2 microglial cells by inhibiting lipopolysaccharide-induced nitric oxide. This cytoprotective effect was confirmed by trypan blue staining, which was used to test for cellular damage from H$_2O_2$. In conclusion, this study highlights the anti-menopausal and antioxidant effect of SISO in an ovariectomized rat model, as well as in microglial cells, and provides new clinical targets for the screening of phytoestrogens as potential candidates for HRT in menopausal women.

Uterine Leiomyosarcoma in a Dog (개에서 자궁 평활근육종의 발생례)

  • Kwon, Jean;Cho, Jong-Ki;Ahn, Gook-Jun;Goo, Ja-Min;Kim, Dae-Yong;Lee, Byeong-Chun;Hwang, Woo-Suk
    • Journal of Veterinary Clinics
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    • v.17 no.2
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    • pp.495-498
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    • 2000
  • Leiomyosarcoma is defined as a malignant smooth muscle tumor or a tumor that arises from smooth muscle and it is found as firm. white and lobulated mass. Canine uterine Ieiomyosa- rcomas are rarely associated with clinical signs. We surveyed(one case which was treated at veter- inary medical leaching hospital of Seoul National University. Through this report we studied the meshed fur diagnosis of uterine leiomyosarcoma by symptom, hematological method and ultrahonog- raphy. To determine uterine leiomysarcoma is difficult due to similarity of clinical signs to pyometra. Especially symptoms could not be noticed even by dogs owners until dogs have an elevation of the number of white blood cells. We conclude ultrasonographic observation and experimental laparotomy are of great value to diagnose the uterine liomyosarcoma.

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Induction of twinning in Korean native cattle by transfer of nuclear transplanted embryos I. Embryo cloning using ovum pick-up(OPU), electric cell fusion and in vitro culture system (핵이식 기법을 이용한 한우 쌍태생산에 관한 연구 I. Ovum pick-up(OPU), 전기적 세포융합 및 체외배양 기법을 이용한 복제수정란 생산)

  • Hwang, Woo-suk;Shin, Tae-young;Roh, Sang-ho;Lee, Byeong-chun
    • Korean Journal of Veterinary Research
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    • v.38 no.1
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    • pp.209-216
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    • 1998
  • The purpose of present study is to improve the efficiency of fusion and the developmental rates of nuclear transplanted embryos to produce genetically identical twins from Korean native cattle. The diameter of aspirated follicles had no significant effect on the recovery rates of oocytes collected by ovum pick-up technique. The fusion rates of nuclear transplanted embryos were significantly higher in 50 and $100{\mu}s$ DC duration groups(73.3 and 72.0% ; respectively) than that in $30{\mu}s$ group(55.6% ; p<0.05). The cleavage rates of nuclear transplanted embryos appeared to be significantly higher in donor nuclei derived from in vivo (65.0%) than in those from in vitro (50.5% ; p<0.01), but the developmental rates to morulae and blastocysts were not significantly different between them(13.7 vs 10.9%, respectively).

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Prediction of Suitable Site to Measure Abdominal Skin Fold Thickness and Correlation among Obesity Indicators in Patients with Type 2 Diabetes Mellitus (제2형 당뇨병 환자에서 피부두겹두께의 측정부위 예측 및 비만지표들 간의 관련성)

  • Hwang, Moon Sook
    • Journal of Korean Biological Nursing Science
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    • v.22 no.1
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    • pp.36-44
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    • 2020
  • Purpose: The purpose of this study was to predict measuring site suited for abdominal skin fold thickness (ASFT) by measuring the distribution of abdominal subcutaneous fat thickness (AScFT) and ascertain the correlations among obesity indicators. Methods: The size of analysis materials was 124 secondary data measured by ultrasonic device, bioelectrical impedance analyzer and caliper. Data were analyzed using t-test, and Pearson's correlation. Results: The average of AScFT was 10.63± 6.79mm with its range 1.39-36.16 mm, and AScFT of female and of central parts were thicker than those of male and outer parts in the abdomen. The average of ASFT was 29.26±12.59 mm. Site 5 on Figure 1 was most similar to the average of AScFT in both sexes. Body mass index (BMI) and waist hip ratio (WHR) were 23.65±3.98 and 0.88±0.05 respectively. The body weight, BMI, WHR, visceral fat, ASFT vs AScFT revealed in significant correlation (r= .29, r= .55, r= .39, r= .33. r= .29). Conclusion: BMI and WHR seem more useful than other obesity indicators, when obesity control is necessary for Type 2 diabetes patients. Site 5 on Figure 1 is most suitable site to measure ASFT.

The Effect of Temporomandibular Joint Chuna Manipulation and Warm Needle Acupuncture at SI19 on Chronic Tinnitus; a Case Report (측두하악관절 추나요법과 청궁혈 온침으로 호전된 만성 이명 환자 1례 보고)

  • Hwang, Eui-Hyoung;Sul, Jae-Uk;Shin, Byung-Cheul;Shin, Mi-Suk;Cho, Hyun-Woo
    • Journal of Korean Medicine Rehabilitation
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    • v.21 no.3
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    • pp.71-78
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    • 2011
  • Objectives : The purpose of this study is to report the combination effect of Chuna manipulation and warm needle acupuncture for a case of obstinate chronic tinnitus. Methods : We applied Danmuji Anchu Tracton technique, lateral pterygoid Chuna and warm needle acupuncture at SI19 once a day for 2 weeks. Visual analogue scale(VAS) was used for the intensity of tinnitus and tinnitus handicap inventory(THI) was evaluated. Results : VAS was improved from 6 to 2 in right ear, 4 to 1 in left ear after the treatment. THI was also improved from 54 to 34 after the treatmemt. Conclusions : The combined therapy of Chuna manipulation and warm needle acupuncture can be used for chronic tinnitus.

Long-term load cycle test of the HTS cable system (초전도케이블의 장기 부하변동시험 및 성능평가)

  • Lim, Ji-Hyun;Sohn, Song-Ho;Yim, Seong-Woo;Sung, Tae-Hyun;Hwang, Si-Dole;Choi, Ha-Ok;Yang, Hyung-Suk;Ma, Yong-Hu;Ryu, K.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.300-300
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    • 2008
  • 우리나라의 전기에너지 수요가 지속적으로 증가함에 따라 새로운 송배전 선로의 구축이 필요하다. 하지만 현재 도심지의 전력구에는 더 이상의 지중선의 증설은 불가능하며 가공선 설치의 경우 환경 민원의 부담을 떠안게 된다. 그래서 기존 동도체의 회선당 송전 한계를 극복하고 대용량 송전 및 손실을 크게 줄일 수 있는 초전도 케이블의 연구가 세계적으로 활발히 진행되고 있다. 한국전력 전력연구원은 신 전력시스템으로서의 22.9 kV급 초전도케이블 시스템을 고창전력시험센터에 설치하고 운영 및 유지 보수 과정을 통하여 실제 계통 적용을 위한 최적 운전조건을 연구하고 있다. 이 논문에서는 실제 부하 상황을 모의하기 위하여 초전도케이블의 장기 부하변동 시험에 따른 케이블 송전 능력 확인 및 냉각시스템의 반응성을 살펴보고 부하변동 시험 후 초전도케이블 도체 열화 여부를 확인하기 위하여 직류 임계전류를 측정하였다. 그 결과 부하변동 시험 전의 임계전류 값과의 변화율이 삼상 모두 ${\pm}1$% 내외로서 오차범위를 벗어나지 않으므로 건전한 것으로 판단되었다.

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