• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.333-336
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• 2001

An isolated phbC gene from Alcaligenes latus was reintroduced into the parent A. latus through the transformation process, and the effect of the amplified phbC gene on the biosynthesis of P(3-hydroxybutyrate-3-hydroxyvalerate) [P(3HB-3HV)] and P(3-hydroxybutyrate-4-hydroxybutyrate) [P(3HB-4HB)] in the transformant A. latus was investigated. The biosynthesis rate and content of the above copolymers increased up to 1.3-fold after enforcing its own phbC gene, and the molar fractions of 3HV and 4HB in P(3HB-3HV) and P(3HB-4HB) also changed remarkably from 35.0 to 48.0% and from 34.0 to 56.0%, respectively, showing a critical role of PHB synthase which catalyzes the polymerizing reactions between eiher 3HV or 4HB from precursor compounds and 3HB.

• Journal of Microbiology and Biotechnology
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• 제7권6호
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• pp.391-396
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• 1997

Two series of carbon sources, linear primary $C_1$~$C_9$ alcohols and linear $C_2$~$C_{10}$ monocarboxylic acids were tested for PHA synthesis in Paracoccus denitrificans. The results showed that the growth-associated synthesis of PHA could be referred only to the carbon sources with odd number of carbon except methanol. For all carbon sources with even number of carbon, nitrogen limitation was required to induce PHA synthesis in P. denitrificans. Poly(3-hydroxyvalerate)[P(3HV)] homopolymer was synthesized from $C_5$, $C_7$, and $C_9$ while growing in the presence of nitrogen, but the nitrogen depletion in the later growth period incorporated 3-hydroxybutyrate(3HB) unit into the polymer chain. The optimum C/N ratio for P(3HV) homopolymer production was found to be 10 when the strain was grown on 10 ml/l of valeric acid for 96 h. P. denitrificans synthesized P(3HB-co-3HV) copolymers from n-hexanoic and n-octanoic acid. The microstructural characterics of the P(3HB-co-3HV) copolymer from n-propanol was investigated using $^13C$-nuclear magnetic resonance spectroscopy, showing a structural heterogeneity.

• 대한수의학회지
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• 제44권1호
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• pp.99-103
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• 2004

The aim of the present study was to develop a sensitive and specific assay for the diagnosis of alcelaphine herpesvirus 1 (AlHV-1) which is a cause agent of malignant catarrhal fever in ruminants. A1HV-1 is a gamma herpesvirus, which is frequent latent, and it is often difficult to detect its antigens or specific nucleic acids because of its low genomic copies in the infected tissues. In this study, polymerase chain reaction (PCR)-dot blot hybridization (DBH) assay for detecting AlHV-1 DNA was developed and evaluated for its sensitivity and specificity as comparison with PCR and DBH alone. The developed PCR-DBH was more sensitive than PCR or DBH alone and also very specific. The results showed that the sensitivity of PCR-DBH were higher and stronger than those of PCR and DBH alone. This PCR-DBH assay can be applied efficiently to confirm the presence of AlHV-1 virus on clinical samples and to differentiate specifically between AlHV-1 infection and other viral infections.

• 한국자동차공학회논문집
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• 제13권1호
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• pp.45-50
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• 2005

Stick type ignition coil(called a Cigar coil) is a kind of transformer for an automobile modulaized of distributer and electric cable. A material of cigar coil is PBT $GF30\%$ resin. This is an excellent engineering plastic with both mechanical and electrical properties. When we insert an HV terminal into the PBT $GF30\%$ resin jacket, it breaks the jacket, because the HV terminal is bigger than the jacket. It is a fatal on durability of a part. In this study, We used ANSYS FEM tool in order to stress analysis by contact. In order to automatically estimate possible maximum diameter of an HV terminal, we used an APDL. In the contact part, we considered the relation of the HV terminal's diameter with the amount of stress that occurred. This relation is able to be applied, in part, to the dimensions of the part design.

• 한국동물위생학회지
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• 제37권2호
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• pp.97-100
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• 2014

This study was performed in Korea to get serological information for bovine herpesvirus 1 (BoHV-1), most commonly found in cattle. Antibodies against BoHV-1 were examined by targeting infectious bovine rhinotracheitis (IBR) in unvaccinated and vaccinated cattle, using viral neutralization (VN) test. In 2013, among 261 sera collected from IBR-unvaccinated herds, 7 sera (2.7%) were found seropositive and their VN titers were ranging from 1:4 to 1:32. Among 315 sera collected from IBR-vaccinated herds in large capacity farms, 303 sera (96.2%) were found to be seropositive for BoHV-1 and their VN titers were in the range of 1:4 to 1:2048. It was found that the IBR-vaccinated herds had higher levels of VN titer than IBR-unvaccinated herds. The results indicated that it may be due to heavy vaccination in vaccinated herds and no or a little infection in unvaccinated herds. At the end of the study it was concluded that although the seropositivity in IBR-unvaccinated herds was low, the monitoring of IBR should be continuously practiced to control and prevent the disease because of exportation of living cattle causing its nationwide outbreaks.

• Fisheries and Aquatic Sciences
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• 제25권7호
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• pp.403-408
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• 2022

In April and June 2014, mortalities of goldfish occurred at Korea. The principal signs included pale gills, severely enlarged and softened spleen and kidney and red liver. Moreover, the histopathological characteristics observed mainly in hematopoietic tissues of kidney, gill lamellae and intestine. The predominant histopathological changes were severe necrosis of hematopoietic tissue. In addition, nucleus exhibiting peripherally displaced chromatin were particularly abundant in the kidney of affected fish. The histological examination led to hypothesize the implication of a virus in the mortality. The hematopoietic necrosis herpesvirus (Cyprinid herpesvirus 2, CyHV-2) DNA polymerase gene successfully detected in DNA extracted from kidney and spleen of affected fish using PCR assay and showed 100% identity with already deposited CyHV-2 DNA polymerase gene in NCBI. Artificial infection trials using affected tissues filtrates gave cumulative mortalities of 30% for virus injected goldfish. In the present study, CyHV-2 was detected and identified as the causative pathogen of the epizootic in goldfish in Korea.

• Journal of Veterinary Science
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• 제21권5호
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• pp.72.1-72.10
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• 2020

Background: Fenbendazole, a dewormer drug, is used widely in the clinical treatment of parasite infections in animals. Recent studies have shown that fenbendazole has substantial effects on tumor growth, immune responses, and inflammatory responses, suggesting that fenbendazole is a pluripotent drug. Nevertheless, the antiviral effects have not been reported. Fenbendazole can disrupt microtubules, which are essential for multiple viruses infections, suggesting that fenbendazole might have antiviral effects. Objectives: This study examined whether fenbendazole could inhibit bovine herpesvirus 1 (BoHV-1) productive infection in cell cultures. Methods: The effects of fenbendazole on viral production, transcription of the immediate early (IE) genes, viron-associated protein expression, and the cellular signaling PLC-γ1/Akt pathway were assessed using distinct methods. Results: Fenbendazole could inhibit BoHV-1 productive infections significantly in MDBK cells in a dose-dependent manner. A time-of-addition assay indicated that fenbendazole affected both the early and late stages in the virus replication cycles. The transcription of IE genes, including BoHV-1 infected cell protein 0 (bICP0), bICP4, and bICP22, as well as the synthesis of viron-associated proteins, were disrupted differentially by the fenbendazole treatment. The treatment did not affect the cellular signaling pathway of PLC-γ1/Akt, a known cascade playing important roles in virus infection. Conclusions: Overall, fenbendazole has antiviral effects on BoHV-1 replication.

• 한국어병학회지
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• 제35권2호
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• pp.231-239
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• 2022

In this study, imported ornamental goldfish (Oranda and black moor) with high mortality was investigated with histopathological analysis. Fishes showed clinical symptoms characterized by gill necrosis and cumulative mortality ranging from 60% to 90% within a month after import from Thailand and China in 2021. CyHV-2 was detected in gill, kidney, spleen and liver of goldfishes. Histopathological analysis revealed epithelial necrosis, intranuclear inclusion bodies associated with viral symptoms in epithelial cell, macrophage infiltration and vacuolation in secondary lamella. In addition, disseminated granuloma formation was observed in various organs such as kidney, heart and mesenchymal membranes. These lesions refer to coinfections with CyHV-2 and other pathogens that cause chronic inflammation, which leads to mortality. Therefore, early disease monitoring needs to reduce the mortality when ornamental goldfish is imported.

• 한국작물학회지
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• 제46권3호
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• pp.189-194
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• 2001

추파연맥-옥수수 이모작 작부체계에서 헤어리벳치 녹비 이용의 가능성 및 그 효과를 검정하기 위해 1999년 및 2000년의 2년간 작물시험장 발포장에서 옥수수 수확 후 9월 초순 연맥과 헤어리벳치를 혼파하여 11월 초순 일차적으로 사초를 수확하고 난 후 월동한 벳치를 옥수수의 녹비로 이용하였을 때의 효과를 조사하였는데 다음과 같은 결과를 얻었다. 옥수수 단작에서 벳치단파에 따른 옥수수에 대한 2년 평균 벳치녹비량 6.7cm/ha의 효과는 연맥이모작의 옥수수 질소표준비와 거의 동일하였다. 연맥과 혼파시의 벳치의 녹비 및 녹비질소량은 벳치 단파시의 40%에 불과하여 옥수수 6엽기에 조사한 토양질산태 질소량도 벳치-녹비구 및 연맥-표준질소구보다 반 이하로 감소하였고 식물체 질소함량이 많이 감소하여 출사기 옥수수 질소흡수량이 40-50kg/ha 감소하였다. 그러나 옥수수 6엽기에서의 토양질산태 질소가 무질소비료구의 2배 정도였으며 시험 2년차에는 추비질소가 필요없이 토양 질산태 질소수준인 25mg/kg에 거의 접근하였으며, 출사기에서의 옥수수 질소흡수량이 무질소비료구보다 약 30-40kg/ha 증가하였다. 시험 1년차 연맥+베치-녹비구의 옥수수 전건물중은 벳치-녹비구 및 연맥-표준질소구보다 종실중이 다소 감소하여 약 2 ton/ha 정도의 수량 감소가 있었지만 시험 2년차는 동일하였다. 옥수수 1년차에 약 60kg/ha, 2년차에 20kg/ha 감소하였으며, 연맥-무질소구에 비해서는 1년차 30kg/ha, 2년차 45kg/ha 정도 증가하여 질소화학비료 50-100kg/ha 대체효과를 얻을 수 있었으며, 뚜렷한 녹비의 2년 누적효과를 볼 수 있었다. 따라서 옥수수의 단작시의 헤어리벳치녹비보다는 효과가 적지만 연맥 이모작시에도 연맥과의 혼파에 의한 헤어리벳치의 녹비 및 질소비료 절감효과도 충분히 크며 녹비의 누적효과도 기대할 수 있을 것으로 판단되었다.

• Journal of applied mathematics & informatics
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• 제7권2호
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• pp.553-573
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• 2000

In this paper, we consider hyperstructures (H,·) when H={e,a,b}. We put a condition on (H,·) where e is a unit. We obtain minimal and maximal Hv -groups , semigroups and quasigroups , using Mathematical 3.0 computer programs.