Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Detection of herpesviral hematopoietic necrosis virus (Cyprinid herpesvirus 2, CyHV-2) from goldfish, Carassius auratus (L.) in Korea

  • Jung, Myung-Hwa (Department of Marine Bio and Medical Sciences, Hanseo University) ;
  • Ryu, Je-Won (Department of Aqualife Medicine, Chonnam National University) ;
  • Nikapitiya, Chamilani (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Jung, Sung-Ju (Department of Aqualife Medicine, Chonnam National University)
  • Received : 2022.03.06
  • Accepted : 2022.06.30
  • Published : 2022.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

In April and June 2014, mortalities of goldfish occurred at Korea. The principal signs included pale gills, severely enlarged and softened spleen and kidney and red liver. Moreover, the histopathological characteristics observed mainly in hematopoietic tissues of kidney, gill lamellae and intestine. The predominant histopathological changes were severe necrosis of hematopoietic tissue. In addition, nucleus exhibiting peripherally displaced chromatin were particularly abundant in the kidney of affected fish. The histological examination led to hypothesize the implication of a virus in the mortality. The hematopoietic necrosis herpesvirus (Cyprinid herpesvirus 2, CyHV-2) DNA polymerase gene successfully detected in DNA extracted from kidney and spleen of affected fish using PCR assay and showed 100% identity with already deposited CyHV-2 DNA polymerase gene in NCBI. Artificial infection trials using affected tissues filtrates gave cumulative mortalities of 30% for virus injected goldfish. In the present study, CyHV-2 was detected and identified as the causative pathogen of the epizootic in goldfish in Korea.

Keywords

References

  1. Boitard PM, Baud M, Labrut S, de Boisseson C, Jamin M, Bigarre L. First detection of Cyprinid herpesvirus 2 (CyHV-2) in goldfish (Carassius auratus) in France. J Fish Dis. 2016;39:673-80. https://doi.org/10.1111/jfd.12400
  2. Chang PH, Lee SH, Chiang HC, Jong MH. Epizootic of herpes-like virus infection in goldfish, Carassius Auratus in Taiwan. Fish Pathol. 1999;34:209-10. https://doi.org/10.3147/jsfp.34.209
  3. Claudio C, Pastorino P, Prato R, Burioli EAV, Peletto S, Righetti M, et al. CyHV-2 outbreak associated with Aeromonas spp. in crucian carp (Carassius carassius) in Piedmont (Italy). In: Berillis P, editor. Trends in fisheries and aquatic animal health. Sharjah: Bentham Science; 2017. p. 307-14.
  4. Danek T, Kalous L, Vesely T, Krasova E, Reschova S, Rylkova K, et al. Massive mortality of Prussian carp Carassius gibelio in the upper Elbe basin associated with herpesviral hematopoietic necrosis (CyHV-2). Dis Aquat Organ. 2012;102:87-95. https://doi.org/10.3354/dao02535
  5. Davison AJ, Kurobe T, Gatherer D, Cunningham C, Korf I, Fukuda H, et al. Comparative genomics of carp herpesviruses. J Virol. 2013;87:2908-22. https://doi.org/10.1128/JVI.03206-12
  6. Doszpoly A, Benko M, Csaba G, Dan A, Lang M, Harrach B. Introduction of the family Alloherpesviridae: the first molecular detection of herpesviruses of cyprinid fish in Hungary. Magy Allatorvosok Lapja. 2011;133:174-81.
  7. Fichi G, Cardeti G, Cocumelli C, Vendramin N, Toffan A, Eleni C, et al. Detection of Cyprinid herpesvirus 2 in association with an Aeromonas sobria infection of Carassius carassius (L.), in Italy. J Fish Dis. 2013;36:823-30. https://doi.org/10.1111/jfd.12048
  8. Goodwin AE, Khoo L, LaPatra SE, Bonar C, Key DW, Garner M, et al. Goldfish hematopoietic necrosis herpesvirus (Cyprinid herpesvirus 2) in the USA: molecular confirmation of isolates from diseased fish. J Aquat Anim Health. 2006;18:11-8. https://doi.org/10.1577/H05-007.1
  9. Groff JM, LaPatra SE, Munn RJ, Zinkl JG. A viral epizootic in cultured populations of juvenile goldfish due to a putative herpesvirus etiology. J Vet Diagn Invest. 1998;10:375-8. https://doi.org/10.1177/104063879801000415
  10. Iqbal MM, Tajima K, Ezura Y. Pathogenicity of motile Aeromonas species isolated from fishes with epizootic ulcerative syndrome (EUS) in Southeast Asian countries. Bull Fish Sci Hokkaido Univ. 1999;50:93-100.
  11. Jeffery KR, Bateman K, Bayley A, Feist SW, Hulland J, Longshaw C, et al. Isolation of a Cyprinid herpesvirus 2 from goldfish, Carassius auratus (L.), in the UK. J Fish Dis. 2007;30:649-56. https://doi.org/10.1111/j.1365-2761.2007.00847.x
  12. Jung SJ, Miyazaki T. Herpesviral haematopoietic necrosis of goldfish, Carassius auratus (L.). J Fish Dis. 1995;18:211-20. https://doi.org/10.1111/j.1365-2761.1995.tb00296.x
  13. Luo YZ, Lin L, Liu Y, Wu ZX, Gu ZM, Li LJ, et al. Haematopoietic necrosis of cultured Prussian carp, Carassius gibelio (Bloch), associated with Cyprinid herpesvirus 2. J Fish Dis. 2013;36:1035-9. https://doi.org/10.1111/jfd.12110
  14. Sahoo PK, Swaminathan TR, Abraham TJ, Kumar R, Pattanayak S, Mohapatra A, et al. Detection of goldfish haematopoietic necrosis herpes virus (Cyprinid herpesvirus-2) with multi-drug resistant Aeromonas hydrophila infection in goldfish: first evidence of any viral disease outbreak in ornamental freshwater aquaculture farms in India. Acta Trop. 2016;161:8-17. https://doi.org/10.1016/j.actatropica.2016.05.004
  15. Song HD, Park JS, Kwon SR. Molecular evidence of Cyprinid herpesvirus 2 (CyHV-2) in domestic goldfish Carassius auratus and imported pearlscale goldfish Carassius auratus. Korean J Fish Aquat Sci. 2018;51:383-8. https://doi.org/10.5657/KFAS.2018.0383
  16. Stephens FJ, Raidal SR, Jones B. Haematopoietic necrosis in a goldfish (Carassius auratus) associated with an agent morphologically similar to herpesvirus. Aust Vet J. 2004;82:167-9. https://doi.org/10.1111/j.1751-0813.2004.tb12650.x
  17. Waltzek TB, Kelley GO, Stone DM, Way K, Hanson L, Fukuda H, et al. Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae. J Gen Virol. 2005;86:1659-67. https://doi.org/10.1099/vir.0.80982-0
  18. Wang L, He J, Liang L, Zheng X, Jia P, Shi X, et al. Mass mortality caused by Cyprinid herpesvirus 2 (CyHV-2) in Prussian carp (Carassius gibelio) in China. Bull Eur Assoc Fish Pathol. 2012;32:164-73.
  19. Wiklund T, Dalsgaard I. Occurrence and significance of atypical Aeromonas salmonicida in non-salmonid and salmonid fish species: a review. Dis Aquat Organ. 1998;32:49-69. https://doi.org/10.3354/dao032049