• 인지과학
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• 제1권2호
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• pp.317-346
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• 1989

물체의 외곽형태로부터 개념적 인식을 도출학기 위하여 사람의 직관과 부합하는 인지적 형태분할 방법을 제안하였다. 의미있는 부분과 그들의 관계로써 형태의 구조를 기술한느 표현은 인지능력을 포함하며 보다 인간적인 방법이다. 일반적으로 인고물은 많은 동직선들과 규칙성을 갖는다. 이러한 인공물의 인지적 분할을 위해서 인지실험에서 의거한 많은 규칙들을 동직선성과 규칙성의 맥락에서 적용하였다. 자연물에 관한 인지적 분할은 하나의 오목점에 대하여 선과 점들의 가능한 배치를 분석하여 이루어졌다. 분할선의 길이, 오목점에서 분할선의 수효, 오목점의 근접성, 오목점의 첨예성, 점들의 대응성과 같은 5가지의 평가기준을 가중합으로 하여 각 배치에 대한 비용함수를 계산한다. 이들 기준은 근접성, 대칭성, 단순성과 같은 사람의 지각속성을 반영한다. 가장 적합한 점은 비용함수를 계산하여 3가지 점들의 집합에서 선택된다. 상이한 종류의 형태에 대하여 수행된 실험은 사람의 직관과 호응하는 결과를 보여 주었다.

• 한국자기공명학회논문지
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• 제7권1호
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• pp.37-45
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• 2003

Human CD99 is a ubiquitous 32-kDa transmembrane protein encoded by mic2 gene. Recently it has been reported that expression of a splice variant of CD99 transmembrane protein (Type I and Type II) increases invasive ability of human breast cancer cells. To understand structural basis for cellular functions of CD99 Type II, we have initiated studies on hCD99$\^$TMcytoI/ using circular dichroism (CD) and multi-dimensional NMR spectroscopy. CD spectrum of hCD99$\^$TMytoI/ in the presence of 200mM DPC and CHAPS displayed an existence ${\alpha}$-helical conformation, showing that it could form an ${\alpha}$-helix under membrane environments. In addition, we have found that the cytoplasmic domain of CD99 would form symmetric dimmer in the presence of transmembrane domain. Although it has been rarely figured out the correlation between structure and functional mechanism of hCD99$\^$TMcytoI/, the dimerization or oligomerization would play an important role in its biological function.

• Genomics & Informatics
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• 제13권1호
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• pp.15-24
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• 2015

Fatty acid synthase (FASN, EC 2.3.1.85), is a multi-enzyme dimer complex that plays a critical role in lipogenesis. This lipogenic enzyme has gained importance beyond its physiological role due to its implications in several clinical conditions-cancers, obesity, and diabetes. This has made FASN an attractive pharmacological target. Here, we have attempted to predict the theoretical models for the human enoyl reductase (ER) and ${\beta}$-ketoacyl reductase (KR) domains based on the porcine FASN crystal structure, which was the structurally closest template available at the time of this study. Comparative modeling methods were used for studying the structure-function relationships. Different validation studies revealed the predicted structures to be highly plausible. The respective substrates of ER and KR domains-namely, trans-butenoyl and ${\beta}$-ketobutyryl-were computationally docked into active sites using Glide in order to understand the probable binding mode. The molecular dynamics simulations of the apo and holo states of ER and KR showed stable backbone root mean square deviation trajectories with minimal deviation. Ramachandran plot analysis showed 96.0% of residues in the most favorable region for ER and 90.3% for the KR domain, respectively. Thus, the predicted models yielded significant insights into the substrate binding modes of the ER and KR catalytic domains and will aid in identifying novel chemical inhibitors of human FASN that target these domains.

• BMB Reports
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• 제34권4호
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• pp.355-364
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• 2001

Major human Nucleoside diphosphate kinases (NDPKs) exist as hetero-oligomers, consisting of NDPK-A and NDPK-B, rather than homo-oligomer. To investigate their biological function depending on the oligomeric structure in vivo, we characterized the biochemical properties of cellular NDPK. Cellular NDPKs, which are made up of a unique combination of isoforms, were purified from human erythrocyte and placenta. We found that cellular NDPK and recombinant isoforms NDPKs have their own distinct biochemical properties in autophosphorylation, stability toward heat or urea, and DNA binding. Cellular NDPK was found to have unique characteristics rather than the expected additive properties of recombinant isoforms. The mutations in the dimeric interface of NDPK-B (R34G, N69H or K135L) caused defective DNA binding and simultaneously reduced the enzymatic stability These results suggest that the oligomeric interaction could play a major role in the stability of catalytic domain and might be related to the regulation of various cellular functions of NDPK.

• Bulletin of the Korean Chemical Society
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• 제22권1호
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• pp.77-83
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• 2001

To gain further insight into the relationship between structure and function of glutathione S-transferase (GST), the four cysteine mutants, C14S, C47S, C101S and C169S, of human GST P1-1 were expressed in Escherichia coli and purified to electrophoretic homogeneity by affinity chromatography on immobilized glutathione (GSH). The catalytic activities of the four mutant enzymes were characterized with five different substrates as well as by their binding to four different inhibitors. Cys14 seems to participate in the catalytic reaction of GST by stabilizing the conformation of the active-site loop, not in the GSH binding directly. The substitution of Cys47 with serine significantly reduces the affinity of GSH binding, although it does not prevent GSH binding. On the other hand, the substitution of Cys101 with serine appears to change the binding affinity of electrophilic substrate by inducing a conformational change of the $\alpha-helix$ D. Cys169 seems to be important for maintaining the stable conformation of the enzyme. In addition, all four cysteine residues are not needed for the steroid isomerase activity of human glutathione S-transferase P1-1.

• 디지털융복합연구
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• 제14권1호
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• pp.189-194
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• 2016

최근 카메라, 캠코더 및 CCTV 등의 사용이 활발해지면서 영상 처리 기술의 수요가 급증하고 있다. 특히 키넥트 센서와 같은 깊이(Depth) 카메라를 사용한 3D 영상 기술에 대한 연구개발이 더욱더 활성화되고 있다. 키넥트 센서는 RGB, 골격(Skeleton) 및 깊이(Depth) 영상을 통해 인체의 3D 골격 구조를 실시간 프레임 단위로 획득할 수 있는 고성능 카메라이다. 본 논문에서는 키넥트 센서를 사용하여 인체의 3D 골격 구조를 모션 캡처하고 범용으로 사용되고 있는 모션 파일 포맷($^*.trc$ 및 $^*.bvh$)으로 선택하여 저장할 수 있는 시스템을 개발한다. 또한 본 시스템은 광학식 모션 캡처 파일 포맷($^*.trc$)을 자기식 모션 캡처 파일 포맷($^*.bvh$)으로 변환할 수 있도록 하는 기능을 가진다. 마지막으로 본 논문에서는 키넥트 센서를 사용하여 캡처한 모션 데이터가 제대로 캡처되어졌는지 모션 캡처 데이터 뷰어를 통하여 확인한다.

• 한국실내디자인학회논문집
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• 제13권5호
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• pp.162-172
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• 2004

Architects and designers of the 20th Century made various efforts to establish new design languages reflecting the changes of society, the times, and environment. They used furniture, especially chairs, as controversial items of aesthetic value, society and ideology. Le Corbusier and Alvar Aalto are furniture designers as well as architects, who adopted this ‘spirit of the times’ actively and have greatly contributed to modernism. This study will help us to understand the diversity of design since modernism, by comparing these two designers' furniture design. It also covers the common factors In modern furniture design, and analyses their individuality and likeness In design. The following is a comparison of furniture design by Le Corbusier and Alvar Aalto. Le Courbusier linked International design and Aalto linked Rational design and Organic concept design to their furniture, just as they did in their architecture. They were able to establish the base of modern furniture design by adapting new concepts and pursuing humanism. In structure, Le Corbusier's furniture Is simple and proportional. It demonstrates a sophisticated geometric composition, mechanical beauty. On the other hand, Aalto rationally linked nature with human requirements and his furniture is organic and in harmony with geometric structure. In function, Le Corbusier's furniture is standardized and prefabricated. He designed for the user so they could choose to use the furniture efficiently to suit their needs. In comparison with Le Corbusier, Aalto Invented the ‘Stacking Chair’ which allows a more effective use of space and reflected the structure of the human body to improve the user's comfort. In materials and techniques, Le Corbusier used new materials like metal or leather, and attempted new ways such as welding, prefabrication, and standardization for production. On the contrary, Alto mainly used birch, which is the traditional material in Finland, and tried new bent wood techniques and joining methods.

• 지역연구
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• 제14권2호
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• pp.65-75
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• 1998

Education is widely recognized one of main sources for growth. This paper attempts to incorporate the general recognition into formal regional growth model. The model structure is largely neoclassical. It produces a single good with the two factors, educated labor and non-educated labor, via a constant return production migrating to the region with the higher real wage. The educated labor in a region is accumulated by two sources, migration and physical education capital, while the non-educated labor is by only migration. The paper shows that regional growth equilibrium is characterized as a saddle point. This indicates the presence of the minimum threshold size that must be overcome before a region may grow. It contrasts sharply with results obtained in regional growth models. The paper suggests that regional growth is determined less by the technical characteristics of regional production function characteristics of regional production function but by the stock combination of educated function but by the stock combination of education labor and non-educated labor. Based on this result, the impact of agglomeration economies on regional growth is explored. It is by phase diagram demonstrated that the presence of agglomeration economies do not always lead a region to growth since there still exists the minimum threshold even in the presence of agglomeration economies.

• 한국실내디자인학회논문집
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• 제13호
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• pp.187-196
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• 1997

A Study on the Characteristics of the Modern Chair Design The features of the modern chair design results from the diverse life style unfolded as per the 20th century social structure and the change of the residential space, and new design has been being developed by the scientists and the artists in various fields through their cooperated work in a way. The field of the scientific or technical study and creation of arts can be developed where they can evoke the sympathy in the circumstances under which the various styles of both arts and science meet across, complement crash together, and grow up. The operation which can control in the network of such pluralistic sphere has been needed for a long period and it has been facing the needs from a time and society. The engineering furniture as the counterplan of the above has brought the value of materials into relief to the forms of human life by making the properties of matter the maximum value or changing it. It can be summarized as following characteristics in its materials and forms along as well as the 3 types (handycrafty, mechanical, and ecological expression) in process through the 20th century. 1. Characteristics in Materials elasticity of the chair as per the changes of the flexibility in the steel pipe development of systematic chair using the plasticity of the plywood, plastic, and wire lightweight due to the materials such as aluminum. 2. Formative characteristics formative simplicity for the increase of the function and the quality improvement such as sociality, productivity, environmental and aesthetic nature emphasis on the structure as per the architectural environment and tecniques pursuit of the forms as a container of the human body seeking for the formative values as the cultural symbols coping with the needs from thepluralistic social structure Furniture is not the makeup for convenience sake but most importantexpression as necessaries of our environments. It should identify itself always as per these kind of needs and also it should be able to used to keep the relation of such mutual division, otherwise the purchase of new furniture should be necessary according to every change of the existing situatiov. Our residence doesn't need the specific style but expresses only the properties of the dwellers.

• Applied Microscopy
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• 제26권1호
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• pp.79-93
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• 1996

The differentiation of nail matrix and fine structure of matrix cells were studied with light and electron microscope using specimens from nails of thumb finger in Korean fetuses 14 to 24 weeks old. Fetal nail matrix consisted of two horizontal layers, thicker ventral and thinner dorsal matrices, originating from invagination of epidermis in proximal nail field. Matrix being generally thicker in its distal region than the apex became gradually thickened with increase of the fetal age. Each matrix consisted of single layer of basal cells and multiple layers of squamous cells which are arranged close to and parallel to the central axis of the nail mairix. The process of keratinization of fetal nail matrix was noted to be occured concurrently in the ventral and dorsal matrices along the central axis of matrix toward distal and dorsal direction. Squamous cells became matured with accumulation of tonofilaments, increase of keratohyalin granules, discharge of membrane coating granules, and narrowing of intercellular spaces, thickening of plasma membrane and finally being transformed into horny cells of nail plate. Horny cells of nail plate filled with fibrous elements in the electron dense amorphous substance. These findings of keratinization process of fetal nail matrix appeared to be similar to those of keratinization in epidermis and inner root sheath of the hair. In the nail matrix, however, corresponding region to the keratogenous zone of growing hair follicle was not observed. Vacuolated squamous cells of nail matrix seen on light microscopy was considered to be artefactual product, but squamous cells with condensed small nuclei rarely found adjacent nail plate was considered to be one of the squamous cells with unknown function. Proximal end of nail plate was observed on dorsal surface of nail field distal to the proximal nail fold at 14 and 16 weeks old human embryos. Proximal prolongation of the proximal end of nail plate was occured with advancing fetal age and afterward 21 weeks nail plate invaded into nail matrix. Melanin granule containing cells and Merkel cells were present only on the basal layer of dorsal nail matirx.