• Parasites, Hosts and Diseases
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• 제58권4호
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• pp.457-460
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• 2020

The Pacific tapeworm Adenocephalus pacifcus (syn. Diphyllobothrium pacificum) is a causative agent of diphyllobothriosis occurred in Pacific coast of South America, mainly in Peru. Source of infections are traditional meal from raw or undercooked marine fish such as "cebiche". We confirmed 3 new cases, one including scolex and the other two headless. A strobila 46 cm long without scolex was discharged from an 8-year-old boy before treatment. Specimens were confirmed morphologically by presence of tegumental protuberances on proglottids and small sized eggs. Partial sequence of cytochrome c oxidase subunit 1 gene was congruent with A. pacificus sequences.

• Journal of Nutrition and Health
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• 제30권4호
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• pp.442-450
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• 1997

Conjugated dienoic derivatives of linoleic acid(CLA) are a mixture of positional and geometric isomers of linoleic acid(LA). We previously found that CLA changes the fatty acid profile in chicken eggs and serum by decreasing monounsaturated fatty acids. Studies were conducted to explore the effects of CLA on fatty acid composition. Rabbits were fed a semisynthetic diet with or without CLA(0.5g CLA/rabbit/day) for 22 weeks. Compared to the control, rabbits fed CLA had significantly lower monounsaturated fatty acid levels(palmitoleic acid Cl6 : 1 by 50% and oleic acid Cl8 : 1, by 20%) in plasma lipids. We found similar differences in fatty acid composition in the liver and the aorta. The inhibitory effect of CLA on $\Delta$9 desaturation was confirmed in a human hepatoma cell line, Hep G2. CLA significantly decreased $\Delta$9 desaturation in 4-5 hours as shown by an increase in the ratio of Cl6 : 0 to C 16 1, This is apparently due to a decrease in $\Delta$9 desaturase(stearoyl-CoA desaturase, SCD) activity ; it was decreased more than 50%. These results, along with our previous findings, indicate that CLA is an inhibitor of $\Delta$9 desaturase in the liver.

• 한국어류학회지
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• 제35권2호
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• pp.91-100
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• 2023

본 연구는 자호천에 서식하는 미유기 Silurus microdorsalis의 난 발생 및 자치어의 형태발달을 관찰하여 초기생활사를 연구하였다. 2018년 6월에 친어를 포획하였으며, 수온 18℃, 광주기 14L: 10D에 조건에서 순환여과식으로 사육하였다. 인공 산란 유도를 위해 성숙한 친어 암컷에 Ovaprim (Syndel, Canada) 어체중 1 kg당 0.5 mL, 수컷에 HCG 어체중 1 kg당 10,000 IU를 주사하였고, 15시간 후에 건식법으로 인공 수정하였다. 성숙란은 연한 담황색의 분리 침성란이었다. 수정란의 난경은 2.16±0.06 mm (n=8)였고, 수정 후 181시간 만에 완전히 부화하였다. 수온별 난 발생은 수온 18℃에서 부화율 63.1±2.2%, 기형률 10.0±3.7%로 나타나 18℃가 부화에 적합한 수온이었다. 수온별 발생속도는 18℃에서 181시간, 21℃에서 109시간, 24℃에서 76시간으로 나타났으며, 초기 난 발생이 진행되지 않는 생물학적 영도는 평균 12.2℃로 추정되었다. 부화 직후 자어의 크기는 4.64±0.22 mm (n=8)였고, 부화 후 1일에 빛의 자극에 회피 반응(negative phototaxis)을 보였다. 부화 7일째는 전장 12.47±0.53 mm로 뒷지느러미 기조는 25~30개, 꼬리지느러미 기조는 14~16개가 관찰되었으며 난황이 모두 흡수되어 전기 자어기로 이행하였다. 부화 9일째는 전장 14.13±0.51 mm로 머리와 몸에 흑색 소포체가 90%가량 침착되었고 등지느러미가 생성되어 기조가 1개 관찰되었으며, 척색 말단이 위로 휘어지기 시작하여 중기 자어기로 이행하였다. 부화 15일째는 전장 16.69±0.31 mm로 꼬리지느러미 기조(18개)가 정수로 나타났으며 등지느러미 기조는 2개, 뒷지느러미 기조는 60~63개가 관찰되었으며, 척색 말단은 45℃로 완전히 휘어져 후기 자어기로 이행하였다. 부화 50일째는 전장 28.96±1.10 mm로 꼬리지느러미(18개), 등지느러미(3개), 가슴지느러미(11개), 뒷지느러미 기조(67~73개)가 정수로 나타나 치어기로 이행하였다.

• 한국생활과학회지
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• 제22권5호
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• pp.491-506
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• 2013

The allergenic food labeling system for 12 foods has been operated in Korean school food service since 2012. This study was conducted to evaluate the food allergy status of Korean elementary school children's and their parents' perceptions of allergenic food labeling system. The parents of school aged(6-9yr olds) children in Gyeongbuk area were recruited to assist in this survey. Surveys were conducted by 404 parents. The prevalence of food allergy was 18.1%(73 students) and about one-half of the food allergic children had a family history of allergies, in particular, maternal family history. The major symptoms were related to skin and the major allergenic foods were mackerel, eggs, milk, wheat, crab and tomatoes. The parents eliminated the allergenic food from diet of 43.8% of the children with food allergy. Participants had an average knowledge score of 68% correct. The average knowledge score was higher for parents with food allergic children than for parents with intact children(p<.05). Over 61% of the respondents were not satisfied with allergenic food labeling system operated in school food service. The requirements for the allergenic food labeling system were the front-of-package labeling, conspicuous description and insert of warning sign. The parents estimated that the most important effect of the allergenic food labeling system was the improvement of psychological and physical health in children with food allergy. Because the only prevention method for food allergy is the restriction of allergenic food, the institutional device to expand the food labeling to unpackaged food as well as packaged food and to make people trust the food labeling should be prepared.

• Parasites, Hosts and Diseases
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• 제22권1호
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• pp.43-50
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• 1984

이형흡충류의 하나인 Stellantchasmus falcatu에 의한 인체기생 2례가 praziquantel투약후 성충을 수집함으로써 화인되었다. 환자는 서울에 거주하는 24세 (제 1례) 및 55세 (제 2례) 남여로 제 1페는 설사, 복통 등 소화기증상과 불정맥(arrhythmia) 등 심식증상을 호소하면서 대편내에 광절렬류조충란이 양성이었고 제 2례는 소화불량과 조충류 편절 배출을 호소하였다. 조충감염 치료를 위해 praziquantel 15∼20mg/kg를 투여하고 MgSO4 30g으로 충체배출을 유도한 바 배출된 조충을 각각 확인하였다. 그러나 나머지 설사편을 입체해부현미경으로 세밀히 조사한 결과 제 1례에서는 188마리의 S. falcatus와 다른 3종의 이형흡충류가, 제 2례에서는 5마리의 S. falcatus가 수집되었다. 환자들은 숭어(Mugil cephalus) 등 반염수9을 회로 먹었다고 하며 이들이 감염원일 것으로 추측되었다.

• Journal of Nutrition and Health
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• 제39권8호
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• pp.832-840
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• 2006

This study was performed to investigate the relationship between diet quality and food cost and identify the effects of food cost on healthy diet among Korean adult. Among the subjects of 2001 National Health and Nutrition Survey 1,641 men and 1,765 women aged from 30 to 49 years were selected and their information of dietary intakes, socio-demo-graphic information, and anthropometric data were analyzed. For the analysis, subjects were classified to quartile based on their daily food cost. Food guidelines provided by Korean Nutrition Society was regarded as a model of healthy diet. Subjects in the lowest quartile of food cost consumed inadequate amount of food from all food categories of the guidelines. Many subjects in the highest quartile not only satisfied the recommended amount of the Food Guide but also seemed to overeat high energy density foods. Even in the highest quartile, about 90% of subjects did not satisfied recommended amount of dairy products. According to the calculated cost of healthy diet, the average cost did not seem to be more expensive than the current mean food cost of the subjects, and most cost-sensitive food intake was observed in the meat fish beans eggs category. The implications of study results were: 1) all the high cost diets did not indicate the high quality of diet, 2) most practical matter of healthy diet in low income group seemed whether they could afford the expense of meat and fish, 3) nutrition education was required to most subjects for healthy diet.

• Parasites, Hosts and Diseases
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• 제32권2호
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• pp.117-120
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• 1994

광절열두조충(DiphvIobothrium latum)의 parvum형 [syn. DiphvLlobothrium pnnlum (Stephens, 1908)]에 의한 희귀한 인체 감염 2례를 발견하여 보고하고자 한다. 첫 환자는 46세 된 가정주부로서 대변검사에서 의엽조충류의 충란이 발견되어 프라지콴텔과 하제를 투 여하여 치료한 결과 두절이 있는 길이 120 cm인 열두조충의 완전한 충체 1마리를 회수하였 다. 환자는 충주호 부근의 횟집에서 송어회를 먹은 적이 있다고 하였다. 또 한 명의 환자는 평소 각종 생선회를 즐겨 먹는 22세 남자 의과대학생으로, 대변에서 길이 15 cm 정도의 조 충류 편절이 자연 배출되어 동정을 의뢰해 왔다 자세한 형태학적 검토 결과 충체들은 D. parvum으로 과거 문헌에 기록된 충체와 동일하였으나 D. parvum의 분류학적 위치가 정립되어 있지 않으며, 충체. 충란 및 각 기관의 크기가 광절열두조충보다 작다는 점 이외에 별다른 차이점을 인정할 수 없었다. 따라서 본 충체들을 광절열두조충의 소형(parvum형)으로 동정하였고. 이 유형에 의한 국내 최초의 감염 증례들로 보고하는 바이다.

• Parasites, Hosts and Diseases
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• 제53권6호
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• pp.725-730
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• 2015

We report here an ectopic case of Fasciola hepatica infection confirmed by recovery of an adult worm in the mesocolon. A 56-year-old female was admitted to our hospital with discomfort and pain in the left lower quadrant of the abdomen. Abdominal CT showed 3 abscesses in the left upper quadrant, mesentery, and pelvic cavity. On surgical exploration, abscess pockets were found in the mesocolon of the sigmoid colon and transverse colon. A leaf-like worm found in the abscess pocket of the mesocolon of the left colon was diagnosed as an adult fluke of F. hepatica. Histologically, numerous eggs of F. hepatica were noted with acute and chronic granulomatous inflammations in the subserosa and pericolic adipose tissues. Conclusively, a rare case of ectopic fascioliasis has been confirmed in this study by the adult worm recovery of F. hepatica in the mesocolon.

• BMB Reports
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• 제46권8호
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• pp.404-409
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• 2013

Extracellular superoxide dismutase (EC-SOD) is a metallo-protein and functions as an antioxidant enzyme. In this study, we used lentiviral vectors to generate transgenic chickens that express the human EC-SOD gene. The recombinant lentiviruses were injected into the subgerminal cavity of freshly laid eggs. Subsequently, the embryos were incubated to hatch using phases II and III of the surrogate shell ex vivo culture system. Of 158 injected embryos, 16 chicks (G0) hatched and were screened for the hEC-SOD by PCR. Only 1 chick was identified as a transgenic bird containing the transgene in its germline. This founder (G0) bird was mated with wild-type hens to produce transgenic progeny, and 2 transgenic chicks (G1) were produced. In the generated transgenic hens (G2), the hEC-SOD protein was expressed in the egg white and showed antioxidant activity. These results highlight the potential of the chicken for production of biologically active proteins in egg white.

• Parasites, Hosts and Diseases
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• 제50권3호
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• pp.269-272
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• 2012

Echinostome eggs were detected in 22 (1.1%) of 2,074 people residing in riparian villages along the Mekong River in Khammouane Province, Lao PDR. In order to recover the adult flukes, 9 persons were treated with praziquantel and purged with magnesium salts. A total of 55 echinostome specimens (4 species) were recovered from the 9 persons. Echinostoma revolutum (8 specimens) was recovered from 3 persons, Artyfechinostomum malayanum (8 specimens) was from 2 persons, Echinochasmus japonicus (33 specimens) was from 7 persons, and Euparyphium sp. (6 specimens) was from 1 person. In Lao PDR, only human infections with E. japonicus were previously known. Therefore, the present study describes human infections with E. revolutum, A. malayanum, and Euparyphium sp. for the first time in Lao PDR. These results indicate that the surveyed villages of Khammouane Province, Lao PDR are low-grade endemic foci of echinostomiasis.