• Journal of Nutrition and Health
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• 제48권3호
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• pp.228-235
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• 2015

본 연구는 만 15~19세 남자 고등학생 902명을 대상으로 식행동, 간식 섭취, 식환경, 생활 습관에 대해 설문 조사를 실시하였고, 비만 판정을 위해 신장과 체중을 측정하여 식행동 패턴에 따른 간식 섭취 및 기타 요인과 비만과의 연관성을 분석하였다. 1) 청소년 식생활 지침의 항목을 이용하여 식행동을 평가한 결과 대상자들은 3가지 패턴으로 분류되었다. 식생활과 생활 습관 모두 건강한 패턴과 식생활과 생활 습관 면에서 건강한 요소와 비건강한 요소가 혼재되어있는 혼합 패턴, 그리고 마지막으로 식생활과 생활 습관이 전반적으로 바람직하지 않은 비건강 패턴이었다. 2) 각 패턴 별로 간식 섭취가 차이를 보였는데, 건강한 패턴은 과일, 우유, 호상요구르트의 섭취 빈도가 높았고, 혼합 패턴은 과일이나 호상요구르트 섭취 빈도가 높은 반면 라면, 아이스크림, 탄산음료, 사탕 섭취 빈도도 같이 높았으며, 비건강 패턴은 과일, 우유, 호상요구르트의 섭취 빈도가 다른 두 패턴에 비해 가장 낮은 반면 라면, 단 간식, 탄산음료 등의 간식 섭취 빈도는 가장 높았다. 3) 식환경을 포함한 생활 습관 요인도 패턴마다 상이했는데, 가정 내 식품 비치율은 각 식품 섭취 빈도와 유사하게 건강한 패턴과 혼합 패턴의 가정 내 과일 비치율이 비건강 패턴에 비해 높았다. 또한 혼합 패턴이 건강기능성 식품이나 식이 보충제 복용 비율이 가장 높았고, 비건강 패턴은 식사 속도, 수면 시간은 짧고 스크린 시간이 하루 2시간 이상인 비율이 가장 높았다. 4) 패턴 별 비만과의 연관성은 건강한 패턴을 기준으로 하였을 때, 혼합 패턴의 교차비는 1.11이었으나 유의하지 않았고, 비건강 패턴의 교차비는 1.88로 유의적이었다. 이상의 결과로부터 청소년의 식행동 패턴에 따라 간식 섭취 뿐 아니라 식습관, 생활 습관 요인이 차이를 보였으며 이러한 요인들이 비만과의 연관성에도 영향을 주었다. 식행동은 식품 섭취나 생활 습관 요인들과 서로 상호작용을 하므로 앞으로 청소년 비만 예방 및 관리를 위한 전략으로 적절한 영양 교육과 함께 식행동과 생활 습관을 함께 개선해 나갈 수 있는 방안을 모색하는 것이 필요하다.

• 보존과학연구
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• 통권26호
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• pp.103-126
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• 2005

Coprostanol is a metabolic product of cholesterol, formed by microbial action in the mammalian gut. This chemical compound is the major sterol in human and has been routinely studied as a biomarker of sewage pollution in marine and lacustrinesediments. This has led to the search for coprostanol as a biomarker in archaeologicalsoils, in order to detect the presence of fecal material. In this study, five samples of archaeological soils excavated at Wanggung ri, Iksancity, were used to assess the possibility of using coprostanol as indicators of ancient human activity in archaeological areas. The sampled soils were analyzed MXRD,EDXRF for their physical and chemical properties. And coprostanol was analysed byGC/MSD, using SIM method to detect and quantify specific compound. The results showed the soils were composed of quartz and feldspars, inorganicelement such as $SiO_2$, $Al_2O_3$, $Fe_2O_3$ etc. Moreover, the result from the analysis wasindicated that the specific compound is coprostanol. The coprostanol was determined at $0.16~1.01\mug$/g in the range of concentrations. This finding indicate that clear promise exists for the exploitation of coprostanol as biomarker of ancient human activity inarchaeological survey. Therefore such studies can serve to increase the confidence we place on biomarker-based methodologies for assessing fecal pollution. The application of this methodology has proved a simple and effective way of searching for that pattern in successively more aged deposits either known or suspected to contain fecal material. And the more scientific analysis of the soils will be showed to utility of the area ancient dietary life style, ancient environment.

• 창의정보문화연구
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• 제7권4호
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• pp.235-242
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• 2021

스마트폰은 다양한 고성능의 센서가 포함되어 있으며 센서에서 발생하는 데이터를 이용하여 인간의 활동을 분석하는 연구가 진행되어왔다. 이러한 인간 활동 인식은 생활 패턴 분석, 운동량 측정, 위험 상황 감지 등 다양한 분야에서 활용될 수 있다. 그러나 기존 연구의 경우 인간의 기본 행동의 인식에 초점을 두거나 효율적인 배터리 사용을 위해 최적의 인식 결과를 내는 방법을 연구하는 경우가 많았다. 본 논문에서는 기본 행동에 건강 관리 목적으로 실내 및 실외에서 행해지는 운동 동작을 총 10가지로 정의하여 인식하도록 하였다. 이를 위해 가속도, 자이로 및 위치 센서의 값을 수집하고 데이터 전처리 과정을 거치고, 활동을 인식하기 위해서 SVM 모델 외에 안정적인 성능을 가진 앙상블 기반의 랜덤 포레스트, 그라디언트 부스팅 모델을 결합하여 투표 기반으로 인식 결과를 결정하였다. 그 결과 높은 정확도로 정의된 활동의 인식이 가능하였으며 특히 유사한 종류의 실내 및 실외 운동 활동의 분류가 가능하였다.

• 한국시스템다이내믹스연구
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• 제8권2호
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• pp.51-82
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• 2007

The purpose of this paper is exploring changes in land use pattern when considering environmental carrying capacity. A sustainable development requires a society to define sustainability constraints, environmental carrying capacity. Environmental carrying capacity can be defined as a level of human activity a region can sustain at a desired level of quality of environment. This concept of environmental carrying capacity can be applied to land use to explore sustainable land use pattern. Since land use pattern can affect environment in an important way, exploring sustainable land use pattern within the limit of environmental carrying capacity can suggest useful implications for a sustainable regional management and planning. For this purpose, this paper built the environmental carrying capacity land use model and applied it to the Metropolitan Area, Korea. System dynamics modeling methods was used to build the model. The model developed in this paper consisted of 6sectors; population, housing, industry, land, environment, and traffic sector. The model limits its main focus on the NO2 level as an indicator of quality of environment in Metropolitan Area. Box model was translated into system dynamics model and combined to urban dynamics model to estimate NO2 level, the maximum number of population, industry structure, housing and maximum amount of land use for industrial, housing, and green space that can sustain desirable NO2 level. Metropolitan area was divided into 16 areas and the model was applied to each area. Since NO2 is flowing in and out from each area, model was built to allow this transboundering nature of air pollutants. Based on the model estimation, several policy implications for a sustainable land use pattern was discussed.

• Molecules and Cells
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• 제37권7호
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• pp.526-531
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• 2014

Human PARP family consists of 17 members of which PARP-1 is a prominent member and plays a key role in DNA repair pathways. It has an N-terminal DNA-binding domain (DBD) encompassing the nuclear localisation signal (NLS), central automodification domain and C-terminal catalytic domain. PARP-1 accounts for majority of poly-(ADP-ribose) polymer synthesis that upon binding to numerous proteins including PARP itself modulates their activity. Reduced PARP-1 activity in ageing human samples and its deficiency leading to telomere shortening has been reported. Hence for cell survival, maintenance of genomic integrity and longevity presence of intact PARP-1 in the nucleus is paramount. Although localisation of full-length and truncated PARP-1 in PARP-1 proficient cells is well documented, subcellular distribution of PARP-1 fragments in the absence of endogenous PARP-1 is not known. Here we report the differential localisation of PARP-1 Nterminal fragment encompassing NLS in PARP-$1^{+/+}$ and PARP-$1^{-/-}$ mouse embryo fibroblasts by live imaging of cells transiently expressing EGFP tagged fragment. In PARP-$1^{+/+}$ cells the fragment localises to the nuclei presenting a granular pattern. Furthermore, it is densely packaged in the midsections of the nucleus. In contrast, the fragment localises exclusively to the cytoplasm in PARP-$1^{-/-}$ cells. Flourescence intensity analysis further confirmed this observation indicating that the N-terminal fragment requires endogenous PARP-1 for its nuclear transport. Our study illustrates the trafficking role of PARP-1 independently of its enzymatic activity and highlights the possibility that full-length PARP-1 may play a key role in the nuclear transport of its siblings and other molecules.

• 대한지리학회지
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• 제40권3호
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• pp.310-320
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• 2005

이 연구는 GPS 수신기를 통해 수집된 개인통행자료를 이용하여 통행패턴의 시공간적 특성을 다양한 그래픽으로 요약하고 탐색적 분석을 가능케하는 지리적 시각화 환경을 제안하고 이를 구현 및 평가하는 것을 목적으로 한다 이 연구에서는 시공간입방체(space-time cube)를 바탕으로 시간지리학 개념을 시각적으로 표현하였으며, 시간산포기둥(temporal dispersion cylinder), 평행평면그림(parallel plane plot)과 같은 부가적인 시각화 도구의 구현을 통해 개인통행패턴의 시공간적 속성 및 이동경로 주위지역의 사회경제적$\cdot$자연적 속성을 함께 탐색할 수 있도록 하였다. 또한 사용자와의 상호작용을 통해 개인통행자료에 포함되어 있는 "언제", "어디에서", "무엇을"이라는 세 가지 정보요소들을 동적으로 탐색 및 질의할 수 있는 환경을 제공한다.

• 동아시아식생활학회지
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• 제17권2호
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• pp.192-197
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• 2007

The purpose of this study was to evaluate the energy intake and energy expenditure of obese children. The body weight and height were measured, and the daily energy intake and activity pattern assessed for one day, using the 24 hour recall method and interviews in 83 elementary school children (59 boys and 24 girls). The average age, height, weight, ideal body weight and obesity index in boys and girls were 11.8 years, 144.8 cm, 54.2 kg, 39.9 kg and 35.9% and 11.5 years, 140.8 cm, 48.9 kg, 36.9 kg and 33.7%, respectively. The mean daily energy intakes of boys and girls were 1,670.5 and 1,656.5 kcal; these being 76.4 and 88.5% of estimated energy requirement for Koreans, respectively. The energy intake ratio from carbohydrates, proteins and fats in boys and girls were 61.5:14.7:23.6 and 62.0:14.7:23.7, respectively. The resting energy expenditure, as estimated by the Harris-Benedict, WHO/NAO/FAO and Korean DRI formulae in boys and girls were 1,452.7, 1,239.2 and 1,629.2 kcal and 1,323.9, 1,156.0 and 1,252.4 kcal, respectively. The activity coefficient for both the boys and girls was 1.39. The daily energy expenditure of boys (1,990.7 kcal) was significantly higher than that of girls (1712.1 kcal). No significant correlations were found between the obesity index, energy intake and energy expenditure. These results suggested that a nutrition and exercise program is required to increase the energy expenditure of elementary school children. Further research will be needed to assess multiple dietary factors and dove]lop a standard method for assessing the energy expenditure of obese children.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2002년도 창립10주년기념 및 국립독성연구원 의약품동등성평가부서 신설기념 국재학술대회:생물학적 동등성과 의약품 개발 전략을 위한 국제심포지움
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• pp.113-113
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• 2002

Phylogenetically conserved Bcl-2 family proteins play a pivotal role in the regulation of apoptosis from virus to human. Members of the Bcl-2 family consist of antiapoptotic proteins such as Bcl-2, Bcl-xL, and Bcl-w, and proapoptotic proteins such as BAD, Bax, BOD, and Bok. It has been proposed that anti- and proapoptotic Bcl-2 proteins regulate cell death by binding to each other and forming heterodimers. A delicate balance between anti- and proapoptotic Bcl-2 family members exists in each cell and the relative concentration of these two groups of proteins determines whether the cell survives or undergoes apoptosis. Mcl-1 (Myeloid cell :leukemia-1) is a member of the Bcl-2 family proteins and was originally cloned as a differentiation-induced early gene that was activated in the human myeloblastic leukemia cell line, ML-1 . Mcl-1 is expressed in a wide variety of tissues and cells including neoplastic ones. We recently identified a short splicing variant of Mcl-1 short (Mcl-IS) and designated the known Mcl-1 as Mcl-1 long (Mcl-lL). Mcl-lL protein exhibits antiapoptotic activity and possesses the BH (Bcl-2 homology) 1, BH2, BH3, and transmembrane (TM) domains found in related Bcl-2 proteins. In contrast, Mcl-1 S is a BH3 domain-only proapoptotic protein that heterodimerizes with Mcl-lL. Although both Mc1-lL and Mcl-lS proteins contain BH domains fecund in other Bcl-2 family proteins, they are distinguished by their unusually long N-terminal sequences containing PEST (proline, glutamic acid, serine, and threonine) motifs, four pairs of arginine residues, and alanine- and glycine-rich regions. In addition, the expression pattern of Mcl-1 protein is different from that of Bcl-2 suggesting a unique role (or Mcl-1 in apoptosis regulation. Tankyrasel (TRF1-interacting, ankyrin-related ADP-related polymerasel) was originally isolated based on its binding to TRF 1 (telomeric repeat binding factor-1) and contains the sterile alpha motif (SAM) module, 24 ankyrin (ANK) repeats, and the catalytic domain of poly(adenosine diphosphate-ribose) polymerase (PARP). Previous studies showed that tankyrasel promotes telomere elongation in human cells presumably by inhibiting TRFI though its poly(ADP-ribosyl)action by tankyrasel . In addition, tankyrasel poly(ADP-ribosyl)ates Insulin-responsive amino peptidase (IRAP), a resident protein of GLUT4 vesicles, and insulin stimulates the PARP activity of tankyrase1 through its phosphorylation by mitogen-activated protein kinase (MAPK). ADP-ribosylation is a posttranslational modification that usually results in a loss of protein activity presumably by enhancing protein turnover. However, little information is available regarding the physiological function(s) of tankyrase1 other than as a PARP enzyme. In the present study, we found tankyrasel as a specific-binding protein of Mcl-1 Overexpression of tankyrasel led to the inhibition of both the apoptotic activity of Mel-lS and the survival action of Mcl-lL in mammalian cells. Unlike other known tankyrasel-interacting proteins, tankyrasel did not poly(ADP-ribosyl)ate either of the Mcl-1 proteins despite its ability to decrease Mcl-1 proteins expression following coexpression. Therefore, this study provides a novel mechanism to regulate Mcl-1-modulated apoptosis in which tankyrasel downregulates the expression of Mcl-1 proteins without the involvement of its ADP-ribosylation activity.

• IMMUNE NETWORK
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• 제1권3호
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• pp.221-229
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• 2001

Background: Inactivation of tumor suppressor genes is believed to be important in the development of many human malignancies. Recently, several lines of evidence have indicated that the wild type p53 gene located at 17p13.3, may function as a tumor suppressor gene and that a mutant p53 gene could promote transformation by inactivating normal p53 function in a dominant negative fashion. These broad spectrum of p53 mutation in human cancers provide that mutant p53 and their protein may be potential targets of tumor diagnostic and therapeutic interventions. Method: Colony formation was performed to investigate growth suppressional ability. p53 expression pattern was examined by western blot and p53-mediated transactivation ability was assessed by CAT activity. SNU C2A cells were observed in apoptotic aspects induced by etoposide and $H_2O_2$ treatment, detecting sensitivity on agent, DNA fragmentation through agarose gel, chromatin condensation by fluorescence microscope, and cell cycle distribution by FACS. Result: 1) p53 mutant his179arg ($histidine{\rightarrow}arginine$) detected in SNU C2A cells lost transcriptional activity and growth suppression ability, showing dominant negative effect on its wild type p53. 2) Etoposide-treated SNU C2A cells induced apoptosis, exhibiting dramatic reduction of cell growth, DNA fragmentation, nuclear condensation formation of apoptotic body and increment of sub-G1 cell fraction. 3) Etoposide and $H_2O_2$-treated SNU C2A cells have no high increase of p53 expression and overexpressed p53 protein changed localization, from cytoplasm to nucleus. Also, p53-mediated transcriptional activity was increased by agents-treatment. Conclusion: SNU C2A cells coexpress wild-type and mutant p53 protein induced apoptosis in the condition on DNA damage, through localizational shift from cytoplasm to nucleus of p53 protein rather than the induction of p53 protein. SNU C2A cells derived mutant p53 his179arg abrogated both the growth supression ability and transactivational activity, showing inhibition effect on transcriptional activity of wild type p53, but did not repress the activity of wild type p53 in SNU C2A cells owing to dominant activity of wild type. These cell condition may provide new gene therapeutic implications leading effective antiproliferation of cell when mutant and wild-type p53 protein were co-expressed in cell.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7053-7060
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• 2015

In the present work, novel orange peel was extracted with 100%EtOH (ethanol) and fractionated into four fractions namely F1, F2, F3, F4 which were eluted from paper chromatographs using 100%EtOH, 80%EtOH, 50%EtOH and pure water respectively. The crude extract and its four fractions were evaluated for their total polyphenol content (TPC), total flavonoid content (TFC) and radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Their cytotoxic activity using WST assay and DNA damage by agarose gel electrophoresis were also evaluated in a human leukemia HL-60 cell line. The findings revealed that F4 had the highest TPC followed by crude extract, F2, F3 and F1. However, the crude extract had the highest TFC followed by F4, F3, F2, and F1. Depending on the values of $EC_{50}$ and trolox equivalent antioxidant capacity, F4 possessed the strongest antioxidant activity while F1 and F2 displayed weak antioxidant activity. Further, incubation HL-60 cells with extract/fractions for 24h caused an inhibition of cell viability in a concentration-dependent manner. F3 and F4 exhibited a high antiproliferative activity with a narrow range of $IC_{50}$ values ($45.9-48.9{\mu}g/ml$). Crude extract exhibited the weakest antiproliferative activity with an $IC_{50}$ value of $314.89{\mu}g/ml$. Analysis of DNA fragmentation displayed DNA degradation in the form of a smear-type pattern upon agarose gel after incubation of HL-60 cells with F3 and F4 for 6 h. Overall, F3 and F4 appear to be good sources of phytochemicals with antioxidant and potential anticancer activities.