• Journal of Nutrition and Health
• /
• 제31권4호
• /
• pp.799-808
• /
• 1998

To investigate the antitumor activity of mugwort (Artemisia princeps Pampan), petroleum ether extract of mugwork was partially purified by a silica gel chromatography. Among several fractions, the fraction which was obtained under the elution with acetone, showed potent cytotoxicity against mouse leukemia cell line(Ll210), human colon cancer cell line (HCT-48) and human hepatoma cell line (Hep G2) , but was less effective with normal cell line(mouse embryo cell). Acetone fraction appeared to be glycolipid by Benedict test and the major fatty acids of the lipid were C16 ; 0 , C 18: 3by GC/MS analysis.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
• /
• pp.79-79
• /
• 2000

In this Paper, the PC input device using MEMS gyros and accelerometer is newly developed, so that it can measure rotation rate and linear acceleration of the human body in space. In General, the human motion has 6 degree of freedom but 2 degree of freedom is enough PC monitor with 2D display. Therefore the simple method is proposed to achieve minimum degree of freedom. It is also applied to the PC mouse. This method can be expanded to the input device for internet set-top box or internet TV.

• Journal of Gastric Cancer
• /
• 제14권2호
• /
• pp.67-86
• /
• 2014

Gastric cancer is one of the most common cancers in the world. Animal models have been used to elucidate the details of the molecular mechanisms of various cancers. However, most inbred strains of mice have resistance to gastric carcinogenesis. Helicobacter infection and carcinogen treatment have been used to establish mouse models that exhibit phenotypes similar to those of human gastric cancer. A large number of transgenic and knockout mouse models of gastric cancer have been developed using genetic engineering. A combination of carcinogens and gene manipulation has been applied to facilitate development of advanced gastric cancer; however, it is rare for mouse models of gastric cancer to show aggressive, metastatic phenotypes required for preclinical studies. Here, we review current mouse models of gastric carcinogenesis and provide our perspectives on future developments in this field.

• 한국HCI학회:학술대회논문집
• /
• 한국HCI학회 2009년도 학술대회
• /
• pp.1-5
• /
• 2009

본 연구에서는 몰입형 인간 컴퓨터 상호작용을 위한 햅틱 마우스 시스템을 제작한다. 제작한 햅틱 마우스는 진동감각 뿐 아니라 온열감도 제공하기 때문에 사용자는 더욱 몰입감 있게 가상의 공간을 경험할 수 있다. 진동 감각과 온열감을 제공하기 위하여 편심모터들과 솔레노이드, 그리고 펠티어 엑츄에이터를 이용하였으며 제작한 시스템의 평가를 위하여 레이싱 게임 프로토타입을 구현하여 테스트를 수행한다. 실험결과로부터 본 햅틱 마우스는 가상 공간에서 사용자에게 사실감을 전달함을 알 수 있다.

• 정보과학회 컴퓨팅의 실제 논문지
• /
• 제21권8호
• /
• pp.531-536
• /
• 2015

음성인식은 HCI(Human Computer Interface)분야에서 가장 활발히 연구되고 있는 분야로 음성을 이용하여 디지털 디바이스를 제어하는 것을 목적으로 하고 있으며 마우스는 GUI 컴퓨터 환경에서 가장 널리 사용하는 장치로서 높은 보급률을 자랑하는 컴퓨터 주변기기 중의 하나이다. 본 논문은 스마트폰 환경에서 실시간 모음 음성 인식을 이용한 마우스 제어 방법에 관하여 제안한다. 구현 방법은 스마트폰에서 실시간으로 일정크기의 음성 신호를 입력 받아 핵심 음성 신호를 추출하고 MFCC(Mel Frequency Cepstral Coefficient)를 이용하여 특징을 추출하여 학습되어 있는 코드 북을 이용하여 양자화를 진행하고 HMM(Hidden Markov Model)을 이용하여 해당 모음 단어를 인식한다. 그리고 각 모음에 해당하는 마우스 명령어로 변환하여 화면상의 가상의 마우스를 제어한다. 최종적으로, 우리는 구현된 스마트폰의 앱을 가지고 데스크톱 PC의 화면상에서 다양한 마우스의 동작을 보여준다.

• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
• /
• pp.121-121
• /
• 2003

The standard protocol for the production of transgenic mouse from ES-injected embryo has to process via chimera producing and several times breeding steps, In contrast, tetraploid-ES cell complementation method allows the immediate generation of targeted murine mutants from genetically modified ES cell clones. The advantage of this advanced technique is a simple and efficient without chimeric intermediates. Recently, this method has been significantly improved through the discovery that ES cells derived from hybrid strains support the development of viable ES mice more efficiently than inbred ES cells do. Therefore, the objective of this study was to generate transgenic mice overexpressing human resistin gene by using tetrapioid-ES cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR and cloned into pCR 2.1 TOPO T-vector and constructed in pCMV-Tag4C vector. Human resistin mammalian expression plasmid was transfected into D3-GL ES cells by lipofectamine 2000, and then after 8~10 days of transfection, the human resistin-expressing cells were selected with G418. In order to produce tetraploid embryos, blastomeres of diploid embryos at the two-cell stage were fused with two times of electric pulse using 60 V 30 $\mu$sec. (fusion rate : 93.5%) and cultured upto the blastocyst stage (development rate : 94.6%). The 15~20 previously G418-selected ES cells were injected into tetraploid blastocysts, and then transferred into the uterus of E2.5d pseudopregnant recipient mice. To investigate the gestation progress, two El9.5d fetus were recovered by Casarean section and one fetus was confirmed to contain human resistin gene by genomic DNA-PCR. Therefore, this finding demonstrates that tetraploid-ES mouse technology can be considered as a useful tool to produce transgenic mouse for the rapid analysis of gene function in vivo.

• IMMUNE NETWORK
• /
• 제2권4호
• /
• pp.233-241
• /
• 2002

Background: Monoclonal antibodies (mAb) of rodent origin are produced with ease by hybridoma fusion technique, and have been successfully used as therapeutic reagents for humans after humanization by genetic engineering. However, utilization of these antibodies for therapeutic purpose has been limited by the fact that they act as immunogens in human body causing undesired side effects. So far, there have been several attempts to produce human mAbs for effective in vivo diagnostic or therapeutic reagents including the use of humanized xenomouse that is generated by mating knockout mice which lost Ig heavy and light chain genes by homologous recombination and transgenic mice having both human Ig heavy and light gene loci in their genome. Methods: Genomic DNA fragments of mouse Ig heavy and light chain were obtained from a mouse brain ${\lambda}$ genomic library by PCR screening and cloned into a targeting vector with ultimate goal of generating Ig knockout mouse. Results: Through PCR screening of the genomic library, three heavy chain and three light chain Ig gene fragments were identified, and restriction map of one of the heavy chain gene fragments was determined. Then heavy chain Ig gene fragments were subcloned into a targeting vector. The resulting construct was introduced into embryonic stem cells. Antibiotic selection of transfected cells is under the progress. Conclusion: Generation of xenomouse is particularly important in medical biotechnology. However, this goal is not easily achieved due to the technical difficulties as well as huge financial expenses. Although we are in the early stage of a long-term project, our results, at least, partially contribute the successful generation of humanized xenomouse in Korea.

• 한국가축번식학회지
• /
• 제22권2호
• /
• pp.195-202
• /
• 1998

본 연구에서는 돼지 난자 내에 돼지, 사람, 소 및 생쥐의 정자를 미세 주입한 후 전핵형성과ㅏ 전핵의 이동을 관찰하였다. 핵과 미세소관은 정자 주입 후 간접면역 형광염색을 실시한 후 공초점주사현미경으로 관찰하였다. 돼지 난자 내에 돼지정자를 직접 주입하였을 경우 일반적인 수정과정과 동일하게 정자중편부에서 성상체가 형성되었고, 이 성상체에 의해서 웅성 및 자성 전핵의 이동(44%), 유사분열(3%) 및 2-세포기(13%)까지 정상적인 수정이 이루어지는 것을 관찰할 수가 있었다. 반면에 이종(사람, 소 및 생쥐)의 정자를 돼지난자에 직접 주입하였을 경우 단위발생시 난 활성이 유도된 난자와 같이 난자자체에서 형성된 미세소관에 의해 전핵이 이동(47, 30 및 17%)하는 것을 볼 수가 있었다. 하지만, 접합체 형성 및 2-세포기로의 분리되는 과정은 관찰할 수 없었다. 이러한 결과로 돼지 난자 내에 이종의 정자가 주입되었을 때 정자의 핵은 비록이적으로 전핵으로 발달되고 난자 중심부로 이동된다는 것을 보여주는 것인데, 이때 전핵을 움직이는 것은 정자에서 유래된 중심체에 의한 것이 아니라 난자세포질 자체의 미세소관에 의한 것으로 관찰되었다.

• 동의생리병리학회지
• /
• 제23권6호
• /
• pp.1314-1319
• /
• 2009

In previous study, we have shown that continentalic acid (CA) isolated from Aralia continentalis induced the growth inhibition and apoptosis in HepG2 cells. In this study, we examine the effects of CA from A. continentalis on growth inhibition and apoptosis induction in human leukemia HL-60 and mouse fibroblast NIH 3T3 cell lines. The results demonstrated that CA decreased cell growth of leukemia HL-60 cells but not human HaCaT keratinocytes, assessed with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and LDH (lactate dehydrogenase) assay. Flow cytometric analysis of mouse fibroblast cell lines exposed to CA showed that apoptotic cells increased in a time- and dose-dependent manner. Treatment with CA decreased the number of normal cells and increased the number of early apoptotic and late apoptotic cells in a dose-dependent manner. The induction of apoptosis in mouse cell lines by CA was mediated through the activation of caspase-3, Bak, and Bax and the down-regulation of Bcl-2. Our results suggest that CA efficiently induces apoptosis in human leukemia cells.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권3호
• /
• pp.445-454
• /
• 2003

Concept and establishment of essential amino acids in animals and human beings rendered immeasurable contributions to animal production and human health. In ruminant animals, however, essential amino acids have never been completely established. The present review proposes a hypothesis that histidine may not be an essential amino acid for normal growing cattle (Japanese black) at least at the growing stage after about 450 kg of body weight on the basis of the experimental results of histidinol dehydrogenase activities in some tissues of the cattle together with hints from which the hypothesis was derived. At the same time, histidinol dehydrogenase activities in liver, kidney and muscle of swine, mouse, fowl and wild duck will be shown and the essentiality of histidine in these animals will be discussed. Finally, the essentiality of histidine for adult human will briefly be discussed.