• Title/Summary/Keyword: Human ART center

검색결과 109건 처리시간 0.024초

Integrity of human sperm DNA assessed by the neutral comet assay and its relationship to semen parameters and clinical outcomes for the IVF-ET program

  • Chi, Hee-Jun;Chung, Da-Yeon;Choi, Soon-Young;Kim, Jong-Hyun;Kim, Gi-Young;Lee, Jae-Seok;Lee, Hee-Sun;Kim, Myung-Hee;Roh, Sung-Il
    • Clinical and Experimental Reproductive Medicine
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    • 제38권1호
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    • pp.10-17
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    • 2011
  • Objective: To explore potential relationships between sperm DNA integrity and both semen parameters and clinical outcomes. Methods: Semen analysis of 498 samples was performed according to the 2010 criteria of the World Health Organization. The sperm DNA fragmentation Index (DFI) of the semen samples was assessed using a neutral comet assay. Results: Sperm DFI showed a significant correlation with semen parameters, including the patient's age, sperm viability, motility, morphology, and number of leukocytes (p<0.05). The sperm DFI values for asthenozoospermic (15.2%), oligoteratozoospermic (18.3%), asthenoteratozoospermic (17.5%), and oligoasthenoteratozoospermic semen samples (21.3%) were significantly higher than that observed in normozoospermic semen samples (10.5%, p<0.05). A sperm DFI value of 14% was used as a threshold of sperm DFI in assessing whether DNA was highly damaged. In 114 IVF-ET cycles, the fertilization rate of the sperm DFI <14% group (70 cycles, 61.7%) was significantly higher than that observed for the ${\geq}14%$ group (44 cycles, 55.3%), but there was no difference in the other clinical outcomes between the two groups. In the ${\geq}14%$ group, the pregnancy rates of the ICSI cycles (40.0%) and half-ICSI (44.0%) were higher than conventional IVF cycles (30.7%), but the difference was not statistically significant. Conclusion: Along with the conventional semen analysis, the sperm DFI assessed using the comet assay was shown to improve the quality of the semen evaluation. To evaluate the precise effect of ICSI on pregnancy rates in the patients who demonstrate high sperm DFI values, further study is necessary.

3차원 입체영상 센싱, 이미징 및 디스플레이를 위한 집적영상 및 디지털 홀로그래피 기술 (Integral Imaging and Digital Holography Techniques for Three-dimensional Sensing, Imaging and Display (Invited Paper))

  • 김승철;신동학;김은수
    • 한국광학회지
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    • 제25권4호
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    • pp.169-192
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    • 2014
  • 본 논문에서는 기존 스테레오 3D 방식의 문제를 해결할 수 있는 집적영상 및 디지털 홀로그래피 방식을 포함하는 공간영상방식의 3차원 영상기술에 대해 소개하고 최근의 국내외 연구개발 동향에 대해 알아본다. 또한 이를 기반으로 향후 연구개발 방향을 전망한다.

중국 신문학 초기의 프로이드 정신분석학 수용 - 루쉰(魯迅)과 궈모뤄(郭沫若)의 프로이드 정신분석학 이론 수용을 중심으로 (The modern Chinese literature and Freudian psychoanalysis - Focuing acceptance of the Freud's theory psychoanalysis on Lu xun and Guo Mo ruo)

  • 고혜경
    • 비교문화연구
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    • 제37권
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    • pp.81-107
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    • 2014
  • Freud's' discovery of the unconscious' is a great revolution. Because of this, human beings were able to get the opportunity to look into their inner than honest. Lu Xun and Guo Mo Ruo a pair of realism and romanticism literature representative of Chinese modern literature writers. They then absorb the psychoanalytic theory o Freud along with other Western zeitgeist, introduce them widely inliterary theory and creative practice, which was again. Lu Xun was reflected in the human world of the unconscious Freud uncovered on the basis of strict realism literary spirit, Guo Mo Ruo is in accordance with the romantic literary time for the purpose of 'art for art' depicting a man's inner psychological well did. Although Freud spirit they claim to social and literary artistry of literature based on different yarns in the Acceptance of analytical theory, has the characteristics of a common sentiment analysis method to express and describe the unconscious human potential. 5.4 When Lu Xun and Guo Mo Ruo China Journalism writers, including Freud's psychoanalytic theory has embraced the Enlightenment and the old feudal society was a major contribution to want to read exactly what the human inner hearing. Chinese modern literature writers have to accept sometimes positive, sometimes accepting the psychoanalytic theory was intellectually sharp criticism, which could be a great instrument in time to the 5.4 Enlightenment and psychological fiction novel further development.

여대생의 라이프스타일과 네일 아트 서비스에 관한 연구 (A Study on Nail Art Service by College Woman's Life Style)

  • 박주현;박옥련;정윤지
    • 한국생활과학회지
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    • 제16권4호
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    • pp.877-884
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    • 2007
  • Today the number of economically independent women are increasing in Korea, they have a tendency to pay more attention to their appearance in their social life. Female university students show a high tendency of that kind, especially when they prepare for their job interviews. They purchase beauty products, visit beauty salons and skin care centers to improve their looks. Above all, their interest in nail art is amazingly increasing for maintaining their self-image. This study thus aims at surveying the relationship between college woman's life style and their interest in nail care, by analysing life styles of college women pursuing up-to-date style. The results show that an 'egoistic' type has a high interest in and a high frequency of using a nail care center, and 'egoistic' type's demand for the nail service is equal to or quite higher than 'appearance-oriented' type's. That means that the 'egoistic' type seems to pay much attention to how they look, because they think their neat appearance help to accomplish their ego.

집단미술치료가 결손가정아동의 정서에 미치는 영향 -우울, 불안, 충동성 중심으로- (Group Art Therapy Program of Low-income Broken; Effects, on Depression, Anxiety and Impulsivity)

  • 김귀복
    • 한국생활과학회지
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    • 제17권6호
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    • pp.1237-1248
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    • 2008
  • This is to study the effects of the program of group art therapeuties for the low-income bracket children's melancholy and uneasiness. The experimental group is 19 children at their option of all 38 children in P child center at J city, Jeonbuk. The program has been going on after-school hours program at the facilities at 16times from May. 4 to August. 17, 2007. I have made the before-medium-after examination the COD, RCMAS and K-MFF through Repeated Measures ANOVA. The result is as follows, The melancholy of experimental group in comparison with that of control group is reduced by control group 0.42 and experimental group 2.67. And in lower rank group, the melancholy of experimental group for control group is dropped in the same way. In uneasiness, by the decrease of control group 0.10, experimental group 0.33, the uneasiness of experimental group for that of control group is decreased. In all drive, the drive of experimental group for that of control group is decreased by control group 0.12 and experimental group 0.33. And similarly, in all lower field, the drive of experimental group is reduced for that of control group. It is through our help to learn the proper way that they express their experience before the group members, listen to another person's story with attention, recognize their own and other's emotion, and are aware of feeling and action.

Behind and Beyond the Archaeology of the Silk Road: Laboratory Analyses in Eurasia, Some Results, Discussions, and Interpretations for Protohistory and Antiquity

  • Henri-Paul FRANCFORT
    • Acta Via Serica
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    • 제8권2호
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    • pp.53-78
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    • 2023
  • The paper presents some new results illustrating some developments related to the concept of the Silk Road and subsequent methodological reflections. New laboratory results of scientific analyses of plants, minerals, and human remains in combination with more conventional methods of research contribute to a better understanding of the multidirectionality of exchanges in Pre- and Protohistory. Unsuspected long-distance transfers of items, especially of metals (tin) and biological materials (plants, pathogens, etc.) are discovered. Adding ancient DNA and petroglyphs to the vexed question of the Indo-European migrations across Eurasia complexifies the familiar linguistic, historical, and archaeological research landscape. Recent excavations show the impact of the adoption of artistic elements adapted from the Achaemenid arts, far in the steppe world, and up to China. Multidirectional (including North-South lanes) and multidisciplinary approaches leave space and hope for more rigorous scientific modelizations for the archaeology of Eurasia and the Silk Road.

보조생식술 센터에서 ISO 9001 : 2000 품질경영시스템의 도입 및 시행의 효용성 (Efficacy of ISO 9001 : 2000 Quality Management System in Human Assisted Reproductive Technology Center)

  • 전진현;박용석;이형송;김순덕;황선희;한수경;김재호;송인옥;강인수;궁미경
    • Clinical and Experimental Reproductive Medicine
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    • 제34권2호
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    • pp.107-115
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    • 2007
  • 목 적: ISO 9001:2000 품질경영시스템은 국제적인 인중 기준에 의해 제품 및 서비스의 품질을 적절하게 판정하고 이를 향상시키기 위해 시행되고 있다. 본 연구에서는 이러한 품질경영시스템을 보조생식술 센터에 성공적으로 도입하고 적용하는 과정에서 확인된 효용성에 대해 기술하고자 한다. 연구방법: 제일병원 아이소망센터는 2004년 1월부터 ISO 9001:2000 인증을 위한 활동을 시작하였으며, 고객만족도조사와 주기적인 내부심사와 사후심사를 실시하였다. 심사과정에서 확인된 부적합 사항에 대한 시정 및 예방 조치와 이에 따른 효과성을 검증하였으며, 고객불만사항에 대한 지속적인 관리와 개선을 위한 프로젝트를 진행하였다. 결 과: 본 센터는 초일류 불임센터로 성장하고 최적의 품질경영시스템을 확립하기 위한 품질방침을 설정하여, 2004년 6월에 한국품질재단 (Korean Foundation for Quality)으로부터 "체외수정 및 배아이식 시술에 관련된 연구"에 대하여 ISO 9001:2000 인증을 받았다. 이러한 품질방침을 실현하기 위해 품질경영시스템에 적합한 품질매뉴얼, 프로세스, 절차서, 지침서 등에 대한 문서화를 완료하였다. 삼 년 동안의 내부심사와 사후심사에서 각각 140건과 7건의 부적합이 확인되었으며, 이에 대한 시정조치를 실시하였다. 결 론: 본 센터에서는 ISO 품질경영시스템의 도입과 운영을 통해 고객만족도의 향상, 체계적인 문서화를 통해 업무의 투명화와 효율화가 가능하였다. 보조생식술 센터에서의 ISO 품질경영시스템은 보조생식술에 대한 국가적인 관리시스템을 효율적으로 운영하기 위한 기본적인 기관별 경영시스템으로서 활용이 가능할 것으로 생각된다.

생식보조시술시 단백질원으로서 인간난포액의 적합성 및 효율성에 관한 연구;I. 인간난포액이 생쥐난포란의 체외성숙에 미치는 효과 (Studies on the Suitability and Efficiency of Human Follicular Fluid as Protein Supplement in Assisted Reproductive Technology(ART);I. Effect of Human Follicular Fluid on Meiotic Maturation of Mouse Follicular Oocytes In Vitro)

  • 지희준;김동훈;김지연;구정진;장상식;정길생
    • Clinical and Experimental Reproductive Medicine
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    • 제23권1호
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    • pp.87-94
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    • 1996
  • For evaluating the suitability of human follicular fluid(HFF) as protein supplement in ART, this preliminary study was performed to examine the maturation promoting activity of HFF on mouse follicular oocytes in vitro. Mouse follicular oocytes were collected from ovaries of 21-28 day old ICR mice by puncturing the antral follicles with fine needle at 48 hours after PMSG injection. The oocytes were rinsed and cultured in modified Whittingham's $T_6$ medium containing purines or dbcAMP to maintain meiotic arrest, and different concentrations of HFF were added into the culture medium to examine the effect of HFF on releasing the oocytes from the suppressive influence of the meiotic inhibitors. As a control for HFF, the maturation promoting activity of human fetal cord serum(HFCS) was investigated and compared with the activity of HFF. While HFF was separated, by molecular weight(M.W), into high M.W. fraction(M.W>30,000) and low M.W. fraction(M.W<30,000) and the effects of the fractions on meiotic resumption were investigated in the presence of the meiotic inhibitors. Also hormone analysis was performed to compare the content of hormones in HFF with that in HFCS. Same concentrations of HFF and HFCS induced similar germinal vesicle break down(GVBD) rates of the oocytes meiotic arrested by purines(4mM hypoxanthine+0.75mM adenosine), but the extrusion rate of 1st polar body(PB) of the oocytes cultured in HFF(65.0%, P<0.05) was significantly higher than that(51.6%) in HFCS. While, in the presence of 200 M dbcAMP, the maturation promoting activity of HFF (GVBD: 70.5%, $p<10^{-6}$; 1st PB extrusion: 67.1%, $p<10^{-3}$) was significantly greater than that of HFCS(GVBD: 35.2%; 1st PB extrusion: 41.1%). The oocytes cultured in the fraction of HFF containing high M.W. components showed higher meiotic maturation rates than the oocytes cultured in the low M.W. fraction of HFF. Gonadotropins and $E_2$ were known to improve the completion of maturation changes, and the levels of these hormones were higher in HFF than in HFCS. Therefore, HFF was more effective than HFCS to use for promoting meiotic resumption of mouse oocytes in vitro.

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비폐쇄성 무정자증 환자에서 난자내 원형정세포 주입에 의한 임신 및 분만 1례 (A Case of Pregnancy and Delivery by Round Spermatid Injection into Oocytes in Nonobstructive Azoospermia Patient)

  • 조정현;심현남;서주태;이동률;윤현수;백혜란;노성일
    • Clinical and Experimental Reproductive Medicine
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    • 제23권3호
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    • pp.327-331
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    • 1996
  • Normal fertilization and pregnancy by round spermatid was achieved from nonobstructive azoospermia patient to be believed untreatable. Therefore, it is suggested that application of round spermatid in human ART program seems to be new treatment of male infertility. Also, it will be needed further research for evaluated fertilization mechanism by round spermatid injection.

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Hierarchical Graph Based Segmentation and Consensus based Human Tracking Technique

  • Ramachandra, Sunitha Madasi;Jayanna, Haradagere Siddaramaiah;Ramegowda, Ramegowda
    • Journal of Information Processing Systems
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    • 제15권1호
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    • pp.67-90
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    • 2019
  • Accurate detection, tracking and analysis of human movement using robots and other visual surveillance systems is still a challenge. Efforts are on to make the system robust against constraints such as variation in shape, size, pose and occlusion. Traditional methods of detection used the sliding window approach which involved scanning of various sizes of windows across an image. This paper concentrates on employing a state-of-the-art, hierarchical graph based method for segmentation. It has two stages: part level segmentation for color-consistent segments and object level segmentation for category-consistent regions. The tracking phase is achieved by employing SIFT keypoint descriptor based technique in a combined matching and tracking scheme with validation phase. Localization of human region in each frame is performed by keypoints by casting votes for the center of the human detected region. As it is difficult to avoid incorrect keypoints, a consensus-based framework is used to detect voting behavior. The designed methodology is tested on the video sequences having 3 to 4 persons.